LOCUS       pUC57mini_v2-HF24_F2_        2075 bp ds-DNA     circular SYN 12-DEC-2022
DEFINITION  Fragment#2 of a 24-fragment split in lacI/lacZ cassette.
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Na Ke
  TITLE     Direct Submission
  JOURNAL   Exported Nov 22, 2019 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            9..248
                     /standard_name="HF24_F2"
     rep_origin      complement(320..908)
     CDS             complement(1079..1939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="AmpR"
     regulatory      complement(1940..2044)
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 ccaatgatgg tctcggatat tcagccatgt gccttcttcc gcgtgcagca gatggcgatg
       61 gctggtttcc atcagttgct gttgactgta gcggctgatg ttgaactgga agtcgccgcg
      121 ccactggtgt gggccataat tcaattcgcg cgtcccgcag cgcagaccgt tttcgctcgg
      181 gaagacgtac ggggtataca tgtctgacaa tggcagatcc cagcggtcaa aacaggcggc
      241 acgagaccat cggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      301 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      481 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      661 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      721 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      781 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      841 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1021 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1081 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1141 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1201 gtgctgcaat gataccgcga gatccacgct caccggctcc agatttatca gcaataaacc
     1261 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1321 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1381 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1441 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1501 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1561 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1621 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1681 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1741 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1801 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1861 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1921 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1981 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2041 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
