LOCUS       pUC57mini_v2-HF24_F19_        1988 bp ds-DNA     circular SYN 12-DEC-2022
DEFINITION  Fragment#19 of a 24-fragment split in lacI/lacZ cassette.
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Na Ke
  TITLE     Direct Submission
  JOURNAL   Exported Nov 22, 2019 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            9..161
                     /standard_name="HF24_F19"
     rep_origin      complement(233..821)
     CDS             complement(992..1852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="AmpR"
     regulatory      complement(1853..1957)
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 ccaatgatgg tctcggcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
       61 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
      121 atttcttgat gtctctgacc agacacccat caaccgagac catcggaaag aacatgtgag
      181 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      241 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      301 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      361 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      421 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      481 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      541 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      601 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      661 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      721 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      781 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      841 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      901 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      961 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1021 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1081 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagatccac
     1141 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1201 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1261 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1321 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1381 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1441 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1501 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1561 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1621 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1681 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1741 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1801 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     1861 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     1921 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     1981 ctgacgtc
//
