LOCUS       pUC57mini_v2-HF24_F21_        2037 bp ds-DNA     circular SYN 12-DEC-2022
DEFINITION  Fragment#21 of a 24-fragment split in lacI/lacZ cassette.
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Na Ke
  TITLE     Direct Submission
  JOURNAL   Exported Nov 22, 2019 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            9..210
                     /standard_name="HF24_F21"
     rep_origin      complement(282..870)
     CDS             complement(1041..1901)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="AmpR"
     regulatory      complement(1902..2006)
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 ccaatgatgg tctcgaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
       61 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
      121 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
      181 tagtgggata cgacgatacc gaacgagacc atcggaaaga acatgtgagc aaaaggccag
      241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      721 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1141 gagggcttac catctggccc cagtgctgca atgataccgc gagatccacg ctcaccggct
     1201 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1321 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
