LOCUS       pUC57mini_v2-HF24_F23-corrected        2056 bp ds-DNA     circular SYN 16-DEC-2022
DEFINITION  Fragment#23 of a 24-fragment split in lacI/lacZ cassette.
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Na Ke
  TITLE     Direct Submission
  JOURNAL   Exported Nov 22, 2019 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            9..229
                     /standard_name="HF24_F23"
     misc_feature    15
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     rep_origin      complement(301..889)
     CDS             complement(1060..1920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="AmpR"
     regulatory      complement(1921..2025)
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 ccaatgatgg tctcagtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
       61 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      121 ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc
      181 gggatctcga ccgatgccct tgagagcctt caacccagtc agcgagacca tcggaaagaa
      241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1201 agatccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1621 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1681 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1741 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1801 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1861 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1921 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2041 agtgccacct gacgtc
//
