LOCUS       Exported               10918 bp ds-DNA     circular SYN 19-DEC-2022
DEFINITION  IncP-based replicating vector expressing dTomato under tac 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    pAWPSG-Ptet-nCas9-BE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Puri AW, Owen S, Chu F, Chavkin T, Beck DA, Kalyuzhnaya MG, Lidstrom
            ME
  TITLE     Genetic tools for the industrially promising methanotroph 
            Methylomicrobium buryatense.
  JOURNAL   Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
  PUBMED    25548049
REFERENCE   2  (bases 1 to 10918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 2, 2023 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    complement(902..1004)
                     /label=T0 terminator
     misc_feature    986..1009
                     /label=Primer-F
     misc_feature    complement(1034..1657)
                     /label=tetR
                     /note="/vntifkey=21"
     promoter        1658..1730
                     /label=Ptet
                     /note="/vntifkey=30"
     misc_feature    1774..2508
                     /label=APOBEC1
                     /label=N-terminal(BE)
                     /note="/vntifkey=21"
     misc_feature    2509..6609
                     /label=nCas9(D10A)
                     /label=dCas9
                     /note="/vntifkey=21"
     misc_feature    6610..6873
                     /label=UGI
                     /label=C-terminal(BE)
                     /note="/vntifkey=21"
     misc_feature    7066..7433
                     /label=terminator
                     /note="/vntifkey=21"
     misc_feature    7480..7584
                     /label=T1 terminator
     CDS             complement(7724..8539)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8843..9991)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10263..10634)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            10667..10776
                     /label=incP origin of transfer
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 acccgctagg gacgtgaagt cgattccttc gatggttagc aatcaaagac tcagagtgct
      781 gtctggagcg tgaatctaac ggtacgtatc tcgattgctc ggtcgctatt cgcactctgc
      841 gaaagttcgt accgctcatt cactaggttg cgaaaattta aatcgtaatt attggggacc
      901 cctggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      961 tggagttctg aggtcattac tggatctatc aacaggagtc caagactagt cgccagggtt
     1021 ttcccagtca ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca
     1081 attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt
     1141 gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt
     1201 gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat
     1261 ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag
     1321 tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac
     1381 ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc
     1441 cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga
     1501 gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct
     1561 gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc
     1621 tgttaatcac tttactttta tctaatctag acatcattaa ttcctaattt ttgttgacac
     1681 tctatcgttg atagagttat tttaccactc cctatcagtg atagagaaaa gtctagaaat
     1741 aattttgttt aactttaaga aggagatata catatgagct cagagactgg cccagtggct
     1801 gtggacccca cattgagacg gcggatcgag ccccatgagt ttgaggtatt cttcgatccg
     1861 agagagctcc gcaaggagac ctgcctgctt tacgaaatta attggggggg ccggcactcc
     1921 atttggcgac atacatcaca gaacactaac aagcacgtcg aagtcaactt catcgagaag
     1981 ttcacgacag aaagatattt ctgtccgaac acaaggtgca gcattacctg gtttctcagc
     2041 tggagcccat gcggcgaatg tagtagggcc atcactgaat tcctgtcaag gtatccccac
     2101 gtcactctgt ttatttacat cgcaaggctg taccaccacg ctgacccccg caatcgacaa
     2161 ggcctgcggg atttgatctc ttcaggtgtg actatccaaa ttatgactga gcaggagtca
     2221 ggatactgct ggagaaactt tgtgaattat agcccgagta atgaagccca ctggcctagg
     2281 tatccccatc tgtgggtacg actgtacgtt cttgaactgt actgcatcat actgggcctg
     2341 cctccttgtc tcaacattct gagaaggaag cagccacagc tgacattctt taccatcgct
     2401 cttcagtctt gtcattacca gcgactgccc ccacacattc tctgggccac cgggttgaaa
     2461 agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga taagaaatac
     2521 tcaataggct tagctatcgg cacaaatagc gtcggatggg cggtgatcac tgatgaatat
     2581 aaggttccgt ctaaaaagtt caaggttctg ggaaatacag accgccacag tatcaaaaaa
     2641 aatcttatag gggctctttt atttgacagt ggagagacag cggaagcgac tcgtctcaaa
     2701 cggacagctc gtagaaggta tacacgtcgg aagaatcgta tttgttatct acaggagatt
     2761 ttttcaaatg agatggcgaa agtagatgat agtttctttc atcgacttga agagtctttt
     2821 ttggtggaag aagacaagaa gcatgaacgt catcctattt ttggaaatat agtagatgaa
     2881 gttgcttatc atgagaaata tccaactatc tatcatctgc gaaaaaaatt ggtagattct
     2941 actgataaag cggatttgcg cttaatctat ttggccttag cgcatatgat taagtttcgt
     3001 ggtcattttt tgattgaggg agatttaaat cctgataata gtgatgtgga caaactattt
     3061 atccagttgg tacaaaccta caatcaatta tttgaagaaa accctattaa cgcaagtgga
     3121 gtagatgcta aagcgattct ttctgcacga ttgagtaaat caagacgatt agaaaatctc
     3181 attgctcagc tccccggtga gaagaaaaat ggcttatttg ggaatctcat tgctttgtca
     3241 ttgggtttga cccctaattt taaatcaaat tttgatttgg cagaagatgc taaattacag
     3301 ctttcaaaag atacttacga tgatgattta gataatttat tggcgcaaat tggagatcaa
     3361 tatgctgatt tgtttttggc agctaagaat ttatcagatg ctattttact ttcagatatc
     3421 ctaagagtaa atactgaaat aactaaggct cccctatcag cttcaatgat taaacgctac
     3481 gatgaacatc atcaagactt gactctttta aaagctttag ttcgacaaca acttccagaa
     3541 aagtataaag aaatcttttt tgatcaatca aaaaacggat atgcaggtta tattgatggg
     3601 ggagctagcc aagaagaatt ttataaattt atcaaaccaa ttttagaaaa aatggatggt
     3661 actgaggaat tattggtgaa actaaatcgt gaagatttgc tgcgcaagca acggaccttt
     3721 gacaacggct ctattcccca tcaaattcac ttgggtgagc tgcatgctat tttgagaaga
     3781 caagaagact tttatccatt tttaaaagac aatcgtgaga agattgaaaa aatcttgact
     3841 tttcgaattc cttattatgt tggtccattg gcgcgtggca atagtcgttt tgcatggatg
     3901 actcggaagt ctgaagaaac aattacccca tggaattttg aagaagttgt cgataaaggt
     3961 gcttcagctc aatcatttat tgaacgcatg acaaactttg ataaaaatct tccaaatgaa
     4021 aaagtactac caaaacatag tttgctttat gagtatttta cggtttataa cgaattgaca
     4081 aaggtcaaat atgttactga aggaatgcga aaaccagcat ttctttcagg tgaacagaag
     4141 aaagccattg ttgatttact cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa
     4201 gaagattatt tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg agttgaagat
     4261 agatttaatg cttcattagg tacctaccat gatttgctaa aaattattaa agataaagat
     4321 tttttggata atgaagaaaa tgaagatatc ttagaggata ttgttttaac attgacctta
     4381 tttgaagata gggagatgat tgaggaaaga cttaaaacat atgctcacct ctttgatgat
     4441 aaggtgatga aacagcttaa acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa
     4501 ttgattaatg gtattaggga taagcaatct ggcaaaacaa tattagattt tttgaaatca
     4561 gatggttttg ccaatcgcaa ttttatgcag ctgatccatg atgatagttt gacatttaaa
     4621 gaagacattc aaaaagcaca agtgtctgga caaggcgata gtttacatga acatattgca
     4681 aatttagctg gtagccctgc tattaaaaaa ggtattttac agactgtaaa agttgttgat
     4741 gaattggtca aagtaatggg gcggcataag ccagaaaata tcgttattga aatggcacgt
     4801 gaaaatcaga caactcaaaa gggccagaaa aattcgcgag agcgtatgaa acgaatcgaa
     4861 gaaggtatca aagaattagg aagtcagatt cttaaagagc atcctgttga aaatactcaa
     4921 ttgcaaaatg aaaagctcta tctctattat ctccaaaatg gaagagacat gtatgtggac
     4981 caagaattag atattaatcg tttaagtgat tatgatgtcg atcacattgt tccacaaagt
     5041 ttccttaaag acgattcaat agacaataag gtcttaacgc gttctgataa aaatcgtggt
     5101 aaatcggata acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa
     5161 cttctaaacg ccaagttaat cactcaacgt aagtttgata atttaacgaa agctgaacgt
     5221 ggaggtttga gtgaacttga taaagctggt tttatcaaac gccaattggt tgaaactcgc
     5281 caaatcacta agcatgtggc acaaattttg gatagtcgca tgaatactaa atacgatgaa
     5341 aatgataaac ttattcgaga ggttaaagtg attaccttaa aatctaaatt agtttctgac
     5401 ttccgaaaag atttccaatt ctataaagta cgtgagatta acaattacca tcatgcccat
     5461 gatgcgtatc taaatgccgt cgttggaact gctttgatta agaaatatcc aaaacttgaa
     5521 tcggagtttg tctatggtga ttataaagtt tatgatgttc gtaaaatgat tgctaagtct
     5581 gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt actctaatat catgaacttc
     5641 ttcaaaacag aaattacact tgcaaatgga gagattcgca aacgccctct aatcgaaact
     5701 aatggggaaa ctggagaaat tgtctgggat aaagggcgag attttgccac agtgcgcaaa
     5761 gtattgtcca tgccccaagt caatattgtc aagaaaacag aagtacagac aggcggattc
     5821 tccaaggagt caattttacc aaaaagaaat tcggacaagc ttattgctcg taaaaaagac
     5881 tgggatccaa aaaaatatgg tggttttgat agtccaacgg tagcttattc agtcctagtg
     5941 gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat ccgttaaaga gttactaggg
     6001 atcacaatta tggaaagaag ttcctttgaa aaaaatccga ttgacttttt agaagctaaa
     6061 ggatataagg aagttaaaaa agacttaatc attaaactac ctaaatatag tctttttgag
     6121 ttagaaaacg gtcgtaaacg gatgctggct agtgccggag aattacaaaa aggaaatgag
     6181 ctggctctgc caagcaaata tgtgaatttt ttatatttag ctagtcatta tgaaaagttg
     6241 aagggtagtc cagaagataa cgaacaaaaa caattgtttg tggagcagca taagcattat
     6301 ttagatgaga ttattgagca aatcagtgaa ttttctaagc gtgttatttt agcagatgcc
     6361 aatttagata aagttcttag tgcatataac aaacatagag acaaaccaat acgtgaacaa
     6421 gcagaaaata ttattcattt atttacgttg acgaatcttg gagctcccgc tgcttttaaa
     6481 tattttgata caacaattga tcgtaaacga tatacgtcta caaaagaagt tttagatgcc
     6541 actcttatcc atcaatccat cactggtctt tatgaaacac gcattgattt gagtcagcta
     6601 ggaggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag
     6661 caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg
     6721 aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat
     6781 gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat
     6841 agcaacggtg agaacaagat taagatgctc taactcgagt aaggatctcc aggcatcaaa
     6901 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     6961 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatacct
     7021 agggatatat tccgcttcct cggcgaccgg ggcggccgcc tcgaggaagc ttgggcccga
     7081 acaaaaactc atctcagaag aggatctgaa tagcgccgtc gaccatcatc atcatcatca
     7141 ttgagtttaa acggtctcca gcttggctgt tttggcggat gagagaagat tttcagcctg
     7201 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
     7261 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     7321 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     7381 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga actggcgccc
     7441 aatacgcaaa ccgtgaaatt gttatccgct ttaattaaag gcatcaaata aaacgaaagg
     7501 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     7561 gtaggacaaa tccgccgccc tagacagctg ggcgcgccat tgtcgggaag atgcgtgatc
     7621 tgatccttcc actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca
     7681 gcgtaatgct ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga
     7741 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     7801 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     7861 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     7921 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     7981 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     8041 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     8101 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     8161 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     8221 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     8281 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     8341 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     8401 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     8461 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt
     8521 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     8581 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
     8641 cgtggctttc gacttccggc aagctatacg cgccctagaa ttgtcaattt taatcctctg
     8701 tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc
     8761 gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct gaacccccag
     8821 ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg
     8881 cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc
     8941 acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg
     9001 ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct
     9061 tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt
     9121 cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc
     9181 ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg
     9241 tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc
     9301 agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc
     9361 gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga
     9421 cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg
     9481 cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg
     9541 gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct
     9601 tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg
     9661 ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg
     9721 tcatcgactt cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg
     9781 ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg
     9841 tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg
     9901 tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc
     9961 tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc
    10021 acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat
    10081 aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact
    10141 tggtattccg aatcttgccc tgcacgaata ccagctccgc gaagtcgctc ttcttgatgg
    10201 agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa
    10261 agtcatggct ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc
    10321 ttctcttcga tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc
    10381 gggtcgtcgg tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg
    10441 cgggccagct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
    10501 acggccagca ggtaggccta caggctcatg ccggccgccg ccgccttttc ctcaatcgct
    10561 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg
    10621 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
    10681 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
    10741 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
    10801 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
    10861 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gatccgtc
//
