LOCUS       Exported               12000 bp ds-DNA     circular SYN 02-JAN-2023
DEFINITION  IncP-based replicating vector expressing dTomato under tac 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    pAWPSG-Po-nCas9-BE-Ptac-sgRNA(mmoX_1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Puri AW, Owen S, Chu F, Chavkin T, Beck DA, Kalyuzhnaya MG, Lidstrom
            ME
  TITLE     Genetic tools for the industrially promising methanotroph 
            Methylomicrobium buryatense.
  JOURNAL   Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
  PUBMED    25548049
REFERENCE   2  (bases 1 to 12000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 2, 2023 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12000
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1011..3132
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8275..8561
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8275..8353
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8275..8353
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8275..8353
                     /organism="Cloning vector pSEVA221"
                     /mol_type="other DNA"
                     /label=/vntifkey=98
                     /note="/vntifkey=98"
                     /db_xref="taxon:1246698"
     source          8295..8349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8295..8349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8354..8428
                     /organism="Cloning vector pSEVA221"
                     /mol_type="other DNA"
                     /label=/vntifkey=98
                     /note="/vntifkey=98"
                     /db_xref="taxon:1246698"
     source          8354..8428
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8429..8448
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    complement(902..1004)
                     /label=T0 terminator
     misc_feature    986..1009
                     /label=Primer-F
     misc_feature    complement(1035..2726)
                     /label=DmpR
                     /note="/vntifkey=21"
     mRNA            2848
                     /label=+1
                     /note="/vntifkey=54"
     protein_bind    3033..3073
                     /label=IHF
                     /note="/vntifkey=31"
     misc_feature    3175..3909
                     /label=APOBEC1
                     /label=N-terminal(BE)
                     /note="/vntifkey=21"
     misc_feature    3910..8010
                     /label=nCas9(D10A)
                     /label=dCas9
                     /note="/vntifkey=21"
     misc_feature    8011..8274
                     /label=UGI
                     /label=C-terminal(BE)
                     /note="/vntifkey=21"
     promoter        8315..8343
                     /label=Ptac
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_feature    8354..8428
                     /label=Ribozyme
     misc_feature    8429..8448
                     /label=sgRNA(mmoX_1)
     misc_feature    8449..8530
                     /label=sgRNA backbone
     misc_feature    8562..8666
                     /label=T1 terminator
     CDS             complement(8806..9621)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9925..11073)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11345..11716)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            11749..11858
                     /label=incP origin of transfer
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 acccgctagg gacgtgaagt cgattccttc gatggttagc aatcaaagac tcagagtgct
      781 gtctggagcg tgaatctaac ggtacgtatc tcgattgctc ggtcgctatt cgcactctgc
      841 gaaagttcgt accgctcatt cactaggttg cgaaaattta aatcgtaatt attggggacc
      901 cctggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      961 tggagttctg aggtcattac tggatctatc aacaggagtc caagactagt agcggataac
     1021 aatttcacac aggattagcc ttcgatgccg attttcttca gccgataggc cagtgccggt
     1081 cggcttaggc cgagcaagcg cgcggcaccg gagacgtttt ggttggcctg ttgcatggct
     1141 ttgcgcatca ggctttcctc gatctcgtcg aggctcaggc cgctgctcaa caactggctg
     1201 atccaactgc cctggccatt gcctggctga atcagcacgc cgtccgacga cacccgctcg
     1261 ctggcggtct gcggctcttc cggcgcccgt gggaacagcg cctgcacgct gatgctttcg
     1321 ttcggatcgg tgaggatgat gccgcgctcg atgacgttct ccagctcacg gatattgccc
     1381 ggccaactgt aatgcaggca ggcctccagg gctttgtctg aaaggccgag ggttctcttg
     1441 ccgtactcct ggtggaagcg ctgaaggaag tgctcaacca gcagtggaat gtcctcgcgg
     1501 cgttcgcgca acgccgggat cgccaccggg aaaacgttca gccggtagta caggtcggcg
     1561 cgaaaacgcc cggccttgac cgcttcggcc aggtcctcgt gggtggctgc gataaccctt
     1621 acgtcgatct tgcgcgtgcg gttgtcgcca actcgctcca gctcgccttc ttgcagcacg
     1681 cgcagcagac tggcctgagc gcgcgggctg agttcgatca cctcgtcaag gaagatggtg
     1741 cccttgtcgg cccgctcgaa gcggcccatg cgtgactggg tggcgccggt gaaggcgcct
     1801 ttttccacgc cgaacaattc ggactcgatc aggtccggcg ggatcgccgc acagttcacc
     1861 gcgacaaagg gctcggcggc gcgtttgctg cgcaggtgca cgctacgcgc gatgacctcc
     1921 ttgccgaccc cggtctcgcc aagcagcagc accgagactt tgccctgtgc ggccttgtcc
     1981 atcatattgc gcacggtctg gtaggccggg gtctgaccga tgccgtagta ctggccttcc
     2041 tgtttgtcga ggttggtacg cagcgacacc agttgcgatt gcaactcgta gagttcctcg
     2101 atgatggggt cgttcttgaa atactgtttg aagctggcaa cgtcgtccca ctcttcggcc
     2161 ggcttgccaa tgacccggca cttgtcgccg ccgcagccgc ggcagctgac ttccttgaag
     2221 atgatttccc ggcccatgaa cgccgaggaa taggcgcagg cgtagccgag cagagtccag
     2281 cacaccgggt cttgcatctg ccccaggtcg gtctggcaga tttccacctc gaaggagtcg
     2341 atccactcca tctcggcata gaagcgcccg cattccttgt cgatatcgac ctcggtgggg
     2401 cggaccttga ccagaccctt gagcgaatgc agctgcgggc cggcgaggaa catgtcgtac
     2461 tcgctggcat tcggtctaag cttcctggcc agttcggcat ccttcaggcc ggactggtaa
     2521 ccctggcgca ggaagaagcc cttggcgcgt tcgatgccca gggtattgac catttcccgg
     2581 cgaaagctgg ccattgctga aacctgcagc aacagcatgc gttgttcgcc aagccagatc
     2641 ttgccttccg tgctctggaa gtggatcagg ttggtcaggt ccttgaaatc ggagtgctgg
     2701 atttcaggct tgtacttgat cggcatgtaa gcgaggcccc tatttatttt tagatgggga
     2761 aatcagggtc gccgctatag cgcaaggcag gcggcgattc cagatggggt catggggaaa
     2821 atcggcagtt ttttcacctg cgccagctcc ccatagccga cctcagtatc tggcaaaagt
     2881 caaccaaatg atcaatcgac ggcagtgact ttagtgctag agatcagcct tcagctgcgc
     2941 catgagcatt tgctcaagcg gccttgggca attgatcaaa tgcttaaaaa gcctgcgcaa
     3001 gcgcggctta atttcgctcg ctccgatcat tctaaaaatt agaaacacat tgaaaaacaa
     3061 taccttgaag tcggttttca gaccttggca cagctgttgc actttgtcct gcgcaatccg
     3121 ccaacctgga gatctagaaa taattttgtt taactttaag aaggagatat acatatgagc
     3181 tcagagactg gcccagtggc tgtggacccc acattgagac ggcggatcga gccccatgag
     3241 tttgaggtat tcttcgatcc gagagagctc cgcaaggaga cctgcctgct ttacgaaatt
     3301 aattgggggg gccggcactc catttggcga catacatcac agaacactaa caagcacgtc
     3361 gaagtcaact tcatcgagaa gttcacgaca gaaagatatt tctgtccgaa cacaaggtgc
     3421 agcattacct ggtttctcag ctggagccca tgcggcgaat gtagtagggc catcactgaa
     3481 ttcctgtcaa ggtatcccca cgtcactctg tttatttaca tcgcaaggct gtaccaccac
     3541 gctgaccccc gcaatcgaca aggcctgcgg gatttgatct cttcaggtgt gactatccaa
     3601 attatgactg agcaggagtc aggatactgc tggagaaact ttgtgaatta tagcccgagt
     3661 aatgaagccc actggcctag gtatccccat ctgtgggtac gactgtacgt tcttgaactg
     3721 tactgcatca tactgggcct gcctccttgt ctcaacattc tgagaaggaa gcagccacag
     3781 ctgacattct ttaccatcgc tcttcagtct tgtcattacc agcgactgcc cccacacatt
     3841 ctctgggcca ccgggttgaa aagcggcagc gagactcccg ggacctcaga gtccgccaca
     3901 cccgaaagtg ataagaaata ctcaataggc ttagctatcg gcacaaatag cgtcggatgg
     3961 gcggtgatca ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca
     4021 gaccgccaca gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca
     4081 gcggaagcga ctcgtctcaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt
     4141 atttgttatc tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt
     4201 catcgacttg aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt
     4261 tttggaaata tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg
     4321 cgaaaaaaat tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta
     4381 gcgcatatga ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat
     4441 agtgatgtgg acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa
     4501 aaccctatta acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa
     4561 tcaagacgat tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt
     4621 gggaatctca ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg
     4681 gcagaagatg ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta
     4741 ttggcgcaaa ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat
     4801 gctattttac tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca
     4861 gcttcaatga ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta
     4921 gttcgacaac aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga
     4981 tatgcaggtt atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca
     5041 attttagaaa aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg
     5101 ctgcgcaagc aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag
     5161 ctgcatgcta ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag
     5221 aagattgaaa aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc
     5281 aatagtcgtt ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt
     5341 gaagaagttg tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt
     5401 gataaaaatc ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt
     5461 acggtttata acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca
     5521 tttctttcag gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa
     5581 gtaaccgtta agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt
     5641 gaaatttcag gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta
     5701 aaaattatta aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat
     5761 attgttttaa cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca
     5821 tatgctcacc tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt
     5881 tggggacgtt tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca
     5941 atattagatt ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat
     6001 gatgatagtt tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat
     6061 agtttacatg aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta
     6121 cagactgtaa aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat
     6181 atcgttattg aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga
     6241 gagcgtatga aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag
     6301 catcctgttg aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat
     6361 ggaagagaca tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc
     6421 gatcacattg ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg
     6481 cgttctgata aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag
     6541 atgaaaaact attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat
     6601 aatttaacga aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa
     6661 cgccaattgg ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc
     6721 atgaatacta aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta
     6781 aaatctaaat tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt
     6841 aacaattacc atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt
     6901 aagaaatatc caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt
     6961 cgtaaaatga ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt
     7021 tactctaata tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc
     7081 aaacgccctc taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga
     7141 gattttgcca cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca
     7201 gaagtacaga caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag
     7261 cttattgctc gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg
     7321 gtagcttatt cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa
     7381 tccgttaaag agttactagg gatcacaatt atggaaagaa gttcctttga aaaaaatccg
     7441 attgactttt tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta
     7501 cctaaatata gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga
     7561 gaattacaaa aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta
     7621 gctagtcatt atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt
     7681 gtggagcagc ataagcatta tttagatgag attattgagc aaatcagtga attttctaag
     7741 cgtgttattt tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga
     7801 gacaaaccaa tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt
     7861 ggagctcccg ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct
     7921 acaaaagaag ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca
     7981 cgcattgatt tgagtcagct aggaggtgac tctggtggtt ctactaatct gtcagatatt
     8041 attgaaaagg agaccggtaa gcaactggtt atccaggaat ccatcctcat gctcccagag
     8101 gaggtggaag aagtcattgg gaacaagccg gaaagcgata tactcgtgca caccgcctac
     8161 gacgagagca ccgacgagaa tgtcatgctt ctgactagcg acgcccctga atacaagcct
     8221 tgggctctgg tcatacagga tagcaacggt gagaacaaga ttaagatgct ctaactgctg
     8281 ccaccgctga gcaaagctgc tctgaaatga gctgttgaca attaatcatc ggctcgtata
     8341 atgtgtggac tgcagctgtc accggatgtg ctttccggtc tgatgagtcc gtgaggacga
     8401 aacagcctct acaaataatt ttgtttaaat gcacaggaag tgcaccgtgt tttagagcta
     8461 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     8521 gtgctttttt tgacccgggg cggccgcctc gattaattaa aggcatcaaa taaaacgaaa
     8581 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     8641 gagtaggaca aatccgccgc cctagacagc tgggcgcgcc attgtcggga agatgcgtga
     8701 tctgatcctt ccactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt
     8761 cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc
     8821 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     8881 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     8941 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     9001 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     9061 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     9121 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     9181 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     9241 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     9301 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     9361 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     9421 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     9481 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     9541 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt
     9601 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     9661 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     9721 aacgtggctt tcgacttccg gcaagctata cgcgccctag aattgtcaat tttaatcctc
     9781 tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt
     9841 gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc
     9901 agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca
     9961 ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg
    10021 ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag
    10081 cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag
    10141 cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat
    10201 ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa
    10261 gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc
    10321 cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga
    10381 ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt
    10441 gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc
    10501 gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag
    10561 cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt
    10621 tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc
    10681 cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt
    10741 tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat
    10801 ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc
    10861 ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc
    10921 cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac
    10981 ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg
    11041 cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgac
    11101 tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct tggtgtccag
    11161 ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt ccttctcgta
    11221 cttggtattc cgaatcttgc cctgcacgaa taccagctcc gcgaagtcgc tcttcttgat
    11281 ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc cggccgtttt agcggctaaa
    11341 aaagtcatgg ctctgccctc gggcggacca cgcccatcat gaccttgcca agctcgtcct
    11401 gcttctcttc gatcttcgcc agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc
    11461 gcgggtcgtc ggtgagccag agtttcagca ggccgcccag gcggcccagg tcgccattga
    11521 tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg tagccctggc
    11581 cgacggccag caggtaggcc tacaggctca tgccggccgc cgccgccttt tcctcaatcg
    11641 ctcttcgttc gtctggaagg cagtacacct tgataggtgg gctgcccttc ctggttggct
    11701 tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc
    11761 gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac
    11821 acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc
    11881 aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat
    11941 ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa aagatccgtc
//
