LOCUS       L0T1_in_pBLUNT_for_addg 5677 bp ds-DNA     circular     07-SEP-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(2002..2334)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
     misc_feature    complement(2189..2217)
                     /label=">pNL_oligo_2085"
     misc_feature    complement(2189..2217)
                     /label=">pNL_oligo_2085"
     primer_bind     2198..2226
                     /label=">pNL_oligo_553"
     primer_bind     2198..2226
                     /label=">pNL_oligo_553"
     primer_bind     2206..2222
                     /label=">pNL_oligo_38"
     primer_bind     2206..2222
                     /label=">pNL_oligo_38"
     misc_feature    2335..2349
                     /label="BsaI linker"
     misc_feature    2335..3664
                     /label="Twist block1 check"
     source          2335..4499
                     /label="source"
     misc_feature    2350..2353
                     /label="GG1"
     misc_feature    2354..2406
                     /label="attP C31"
     misc_feature    2407..4432
                     /label="pUBQ10"
     misc_feature    3400..4499
                     /label="Twist part2"
     misc_feature    complement(4433..4487)
                     /label="attB C31"
     misc_feature    4488..4490
                     /label="GG2"
     misc_feature    4491..4499
                     /label="BsaI linker2"
     misc_feature    complement(4500..2001)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
     misc_feature    complement(4527..4541)
                     /label=">pNL_oligo_2447"
     misc_feature    complement(4527..4541)
                     /label=">pNL_oligo_2447"
     misc_feature    complement(4533..4554)
                     /label=">pNL_oligo_1301"
     misc_feature    complement(4533..4554)
                     /label=">pNL_oligo_1301"
     misc_feature    complement(4567..4585)
                     /label=">pNL_oligo_3104"
     misc_feature    complement(4567..4585)
                     /label=">pNL_oligo_3104"
     misc_feature    complement(4568..4584)
                     /label=">pNL_oligo_16"
     misc_feature    complement(4568..4584)
                     /label=">pNL_oligo_16"
     misc_feature    complement(4574..4599)
                     /label=">pNL_oligo_1548"
     misc_feature    complement(4574..4599)
                     /label=">pNL_oligo_1548"
     misc_feature    complement(4592..4614)
                     /label=">pNL_oligo_552"
     misc_feature    complement(4592..4614)
                     /label=">pNL_oligo_552"
     misc_feature    complement(4593..4608)
                     /label=">pNL_oligo_37"
     misc_feature    complement(4593..4608)
                     /label=">pNL_oligo_37"
     primer_bind     4612..4633
                     /label=">pNL_oligo_3070"
     primer_bind     4612..4633
                     /label=">pNL_oligo_3070"
     primer_bind     4612..4635
                     /label=">pNL_oligo_2216"
     primer_bind     4612..4635
                     /label=">pNL_oligo_2216"
     misc_feature    complement(4612..4635)
                     /label=">pNL_oligo_2215"
     misc_feature    complement(4612..4635)
                     /label=">pNL_oligo_2215"
     misc_feature    complement(4615..4635)
                     /label=">pNL_oligo_2699"
     misc_feature    complement(4615..4635)
                     /label=">pNL_oligo_2699"
ORIGIN
        1 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
       61 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
      121 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
      181 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
      241 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
      301 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
      361 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
      421 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
      481 cgccttctat cgccttcttg acgagttctt ctgaattatt aacgcttaca atttcctgat
      541 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc
      601 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      661 cgctcatgag acaataaccc tgataaatgc ttcaataata gcacgtgagg agggccacca
      721 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
      781 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
      841 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
      901 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
      961 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     1021 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     1081 aggagcagga ctgacacgtg ctaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagcta tttaggtgac gcgttagaat actcaagcta tgcatcaagc
     2281 ttggtaccga gctcggatcc actagtaacg gccgccagtg tgctggaatt caggcactct
     2341 gtggtctcac tatagtagtg ccccaactgg ggtaaccttt gagttctctc agttgggggc
     2401 gtagggctca acaacaaact ttccattcac ccatttatgg atacacagtc tagctcaaca
     2461 gagcttttaa cccaaattgg tacaatagaa tacaacttta gatcataatt ctcaaaagaa
     2521 agagattcct tagctattct atctgccact ccatttcctt ctcggcttgt atgcacaagc
     2581 ataaaatcct caaacttgct aagtagatac tttatgtctt ggataattgg attgagactt
     2641 gacaagcata actttcatgt aaccaaagac acaagttgct gagaatccac ctcaaaaatg
     2701 atcttcctat aattgaatcg ggataatgac agcacagccc atctaagagc ctccacttct
     2761 acttccagca cgcttcttac ttttaccaca gctcttgcac ctaaccataa caccttccct
     2821 gtatgatcgc gaagcaccca ccctaagcca cattttaatc cttctgttgg ccatgcccca
     2881 tcaaagttgc acttaaccca agattgtggt ggagcttccc atgtttctcg tctgtcccga
     2941 cggtgttgtg gttggtgctt tccttacatt ctgagcctct ttccttctaa tccactcatc
     3001 tgcatcttct tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac
     3061 cagctcgttt ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt
     3121 cttcagatca ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga
     3181 tgatctagga ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg
     3241 tatcattctt gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg
     3301 agtgttaaat atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag
     3361 aataaatcga gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata
     3421 caaaagtcat tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa
     3481 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     3541 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     3601 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     3661 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     3721 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     3781 gttagaaatt gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg
     3841 cacacacgag tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa
     3901 ggcaattagc caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt
     3961 attagctatt gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc
     4021 ttcttcttct ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt
     4081 tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt
     4141 gttccttatt ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt
     4201 tctttggttt agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag
     4261 atatcatctt aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt
     4321 gagttttgtc gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt
     4381 ctagtttgtg cgatcgaatt tgtcgattaa tctgagtttt tctgattaac agaggtggag
     4441 tacgcgcccg gggagcccaa gggcacgccc tggcacccgc accgcggacc ttgagacctc
     4501 ctgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccca
     4561 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
     4621 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     4681 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctatacg
     4741 tacggcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     4801 tacagagtga tattattgac acgccggggc gacggatggt gatccccctg gccagtgcac
     4861 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     4921 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     4981 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     5041 gaatataaat gtcaggcatg agattatcaa aaaggatctt cacctagatc cttttcacgt
     5101 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct
     5161 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
     5221 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
     5281 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga
     5341 tctgatggcg caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga
     5401 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5461 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5521 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     5581 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5641 acgttgtcac tgaagcggga agggactggc tgctatt
//