LOCUS       L0-I2_pBLUNT-Corrected_ 4182 bp ds-DNA     circular     19-NOV-2019
DEFINITION  .
ACCESSION   New Construct
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     Imported using the Genbank importer. File name: MutatedpPP2AA3
            G0-G1 level0.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..15
                     /label="BsaI linker"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     source          1..19
                     /label="source:Synthetic"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1..19
                     /label="P_fwd"
                     /note="sequence: cactctgtggtctcaggag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    16..19
                     /label="GG0"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    20..651
                     /label="Mutated pPP2AA3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(628..670)
                     /label="Correct_P_GG1"
                     /note="sequence: acgttgatggtctcacatTcctcaccaaaactcaaatcctttg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    653..653
                     /label="GG1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     source          653..670
                     /label="source:Synthetic"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(656..670)
                     /label="BsaI linker3"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    671..1003
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(779..807)
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(779..807)
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     complement(783..799)
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(783..799)
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    788..816
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    788..816
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1004..4182
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4047..4067
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4047..4067
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4047..4070
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4047..4070
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(4047..4070)
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(4047..4070)
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(4049..4070)
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(4049..4070)
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4068..4090
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4068..4090
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4074..4089
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4074..4089
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4083..4108
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4083..4108
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4097..4115
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4097..4115
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4098..4114
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4098..4114
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4128..4149
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4128..4149
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4141..4155
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4141..4155
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 cactctgtgg tctcaggagg aggaagaaga agagagttac tgagaagctt cctttatacg
       61 agagagatat taaacgaagt cgtatttgtt ggatatatgg gcctttatgt attatacata
      121 agcccattta gttcttatct ttgacaggcc caattatatg gacacagctt gaagatggtt
      181 tagctgctgc gaaagacgag ctccggtctg atttctcgtt cttctgattg atagatcgct
      241 cggaacttgg aaagcagcgt aatcggtaaa ttctcgatca tttcttcaat ttcgaaacat
      301 tttccaattg tatattgaga atctcaaatc gaattattgt ttagatcttc tctctttatt
      361 agtctaagaa tggtaatagt ttctctctag attgggacgg cacaaagttt cagttctcca
      421 ttctaatcat tttttagagg ctaatgggtc acctttgaat tatctcagag gcggaattga
      481 gtttaaagtt tgtaacttta tcgtcttttg tattgatggt gtctatggca aaggtgatta
      541 actgcaaagg tatgcattgt gttttattct gactttgtgt acatttgtgt ttgattgaag
      601 gtagggagtg gcaaaggtga ttaactgcaa aggatttgag ttttggtgag gAatgtgaga
      661 ccatcaacgt cctgaattcc agcacactgg cggccgttac tagtggatcc gagctcggta
      721 ccaagcttga tgcatagctt gagtattcta acgcgtcacc taaatagctt ggcgtaatca
      781 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      841 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      901 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      961 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1021 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1081 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1141 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1201 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1261 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1321 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1381 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1441 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1501 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1561 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1621 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1681 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1741 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1801 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1861 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttagcacgtg tcagtcctgc
     1921 tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc
     1981 cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac
     2041 acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg
     2101 gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg
     2161 accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag
     2221 aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg
     2281 gccatggtgg ccctcctcac gtgctattat tgaagcattt atcagggtta ttgtctcatg
     2341 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2401 ccccgaaaag tgccacctga tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2461 ccgcatcagg aaattgtaag cgttaataat tcagaagaac tcgtcaagaa ggcgatagaa
     2521 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     2581 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     2641 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     2701 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc
     2761 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     2821 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     2881 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     2941 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     3001 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     3061 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc
     3121 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     3181 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     3241 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     3301 tcctgtctct tgatcagagc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     3361 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     3421 cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca
     3481 agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     3541 attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgaaaa ggatctaggt
     3601 gaagatcctt tttgataatc tcatgcctga catttatatt ccccagaaca tcaggttaat
     3661 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
     3721 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
     3781 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
     3841 caccatccgt cgccccggcg tgtcaataat atcactctgt acatccacaa acagacgata
     3901 acggctctct cttttatagg tgtaaacctt aaactgccgt acgtataggc tgcgcaactg
     3961 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     4021 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     4081 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggccctc tagatgcatg
     4141 ctcgagcggc cgccagtgtg atggatatct gcagaattca gg
//