LOCUS       L0-I15_pBLUNTrev-tUBQ1_ 4050 bp ds-DNA     circular     17-SEP-2019
DEFINITION  .
ACCESSION   New Construct
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     Imported using the Genbank importer. File name: tUBQ1 GG4-GG6
            level0.gb
FEATURES             Location/Qualifiers
     misc_feature    1..15
                     /label="BsaI linker"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1..49
                     /label="tUBQ1 GG4 fwd"
                     /note="sequence: cactctgtggtctcagcttagagactcttatcaagaatcccatctcttg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     source          1..538
                     /label="source"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    16..19
                     /label="GG4"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    20..519
                     /label="tUBQ1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(494..538)
                     /label="tUBQ1 GG6 rev"
                     /note="sequence: ggatcggaggtctcaagcgcatttgtttgtctttaacaacggctc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    520..523
                     /label="GG6"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(524..538)
                     /label="BsaI linker2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(539..3717)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(566..580)
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(566..580)
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(572..593)
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(572..593)
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(606..624)
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(606..624)
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(607..623)
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(607..623)
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(613..638)
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(613..638)
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(631..653)
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(631..653)
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(632..647)
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(632..647)
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     651..672
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     651..672
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     651..674
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     651..674
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(651..674)
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(651..674)
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(654..674)
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(654..674)
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(3718..4050)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(3905..3933)
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(3905..3933)
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     3914..3942
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     3914..3942
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     3922..3938
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     3922..3938
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 cactctgtgg tctcagctta gagactctta tcaagaatcc catctcttgc ttgctttttt
       61 ttgttgtctt ccctttgata gggtttgttt ttcttgtttc agtgactttc tatgttaaaa
      121 gataatgtca gtaaaaggat ttggttttct attattctga atcgattacg gaagattctt
      181 gcttaattcc aatctataca agtatcgtga aataatgacc gtttatgtga ttaggagacg
      241 tgtttcatta ataaaatata agatcaatac attgttagta gtgataaact atgtacaaat
      301 tgtattgatt gtaaaagaaa cacaataggt tccttttttc tacaatatat tgtgacagac
      361 tctctgtttt aacgaatgaa ttaaatttgt caacgaaaaa attaaagaat gatgtgatca
      421 ctttgcacct tggttcatct tcagaggctt ctgcatgttc catgcattga ggctctctac
      481 ggtcacaggt tgtgagccgt tgttaaagac aaacaaatgc gcttgagacc tccgatcccc
      541 tgaattctgc agatatccat cacactggcg gccgctcgag catgcatcta gagggcccaa
      601 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
      661 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      721 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctatacgt
      781 acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
      841 acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg ccagtgcacg
      901 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
      961 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     1021 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     1081 aatataaatg tcaggcatga gattatcaaa aaggatcttc acctagatcc ttttcacgta
     1141 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     1201 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     1261 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     1321 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     1381 ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgcatgat
     1441 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     1501 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     1561 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     1621 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     1681 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     1741 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     1801 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     1861 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     1921 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     1981 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     2041 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     2101 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     2161 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     2221 gttcttctga attattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     2281 gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2341 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2401 aaatgcttca ataatagcac gtgaggaggg ccaccatggc caagttgacc agtgccgttc
     2461 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     2521 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     2581 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     2641 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     2701 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     2761 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctaa
     2821 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3061 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3601 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3661 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3721 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3781 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     3841 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     3901 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctattta
     3961 ggtgacgcgt tagaatactc aagctatgca tcaagcttgg taccgagctc ggatccacta
     4021 gtaacggccg ccagtgtgct ggaattcagg
//