LOCUS       L1P33_(pLBD16-PhiC31-NL 8016 bp ds-DNA     circular     26-SEP-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     source          3..4661
                     /label="source:synthetic DNA construct"
     misc_feature    complement(23..42)
                     /label=">pNL_oligo_513"
     misc_feature    complement(31..50)
                     /label=">pNL_oligo_514"
     misc_feature    44..68
                     /label="RB T-DNA repeat"
     misc_feature    complement(44..68)
                     /label=">pNL_oligo_3103"
     misc_feature    complement(63..113)
                     /label=">pNL_oligo_1763"
     primer_bind     84..119
                     /label=">pNL_oligo_1764"
     rep_origin      complement(159..747)
                     /label="ori"
     CDS             complement(918..1733)
                     /label="aminoglycoside phosphotransferase"
     primer_bind     1319..1346
                     /label=">pNL_oligo_1293"
     misc_feature    complement(1319..1346)
                     /label=">pNL_oligo_1294"
     primer_bind     1324..1346
                     /label=">pNL_oligo_1590"
     primer_bind     1327..1347
                     /label=">pNL_oligo_1587"
     misc_feature    complement(1367..1403)
                     /label=">pNL_oligo_2197"
     misc_feature    1830..1838
                     /label="DraIII"
     primer_bind     1902..1921
                     /label=">pNL_oligo_1275"
     misc_feature    complement(1902..1921)
                     /label=">pNL_oligo_1276"
     rep_origin      2024..2459
                     /label="pSa ori"
     misc_feature    2594..2616
                     /label="LB T-DNA repeat"
     primer_bind     2621..2646
                     /label=">pNL_oligo_248"
     polyA_signal    2624..2800
                     /label="CaMV poly(A) signal"
     primer_bind     2733..2754
                     /label=">pNL_oligo_1355"
     misc_feature    complement(2830..3005)
                     /label="CaMV terminator"
     misc_feature    complement(2830..4397)
                     /label="Hyg resistance cassette"
     CDS             complement(3010..4035)
                     /label="hygromycin B phosphotransferase"
     primer_bind     3340..3359
                     /label=">pNL_oligo_2766"
     misc_feature    3451..3459
                     /label="DraIII"
     misc_feature    3744..3752
                     /label="DraIII"
     misc_feature    complement(3788..3807)
                     /label=">pNL_oligo_2767"
     primer_bind     3967..3990
                     /label=">pNL_oligo_2672"
     promoter        complement(4052..4397)
                     /label="CaMV 35S promoter"
     primer_bind     4083..4101
                     /label=">pNL_oligo_3106"
     misc_feature    complement(4145..4167)
                     /label=">pNL_oligo_1354"
     misc_feature    4167..4172
                     /label="BpiI"
     misc_feature    complement(4184..4189)
                     /label="BpiI"
     CDS             complement(4395..4661)
                     /label="LacZ-alpha fragment of beta-galactosidase"
     misc_feature    complement(4409..4414)
                     /label="BpiI"
     CDS             complement(4479..4547)
                     /label="LacZ alpha"
     primer_bind     4592..4612
                     /label=">pNL_oligo_2699"
     primer_bind     4592..4615
                     /label="M13F(-47)"
     primer_bind     4592..4615
                     /label=">pNL_oligo_2215"
     misc_feature    complement(4592..4615)
                     /label=">pNL_oligo_2216"
     misc_feature    complement(4594..4615)
                     /label=">pNL_oligo_3070"
     primer_bind     4598..4615
                     /label="M13F(-41)"
     primer_bind     4613..4635
                     /label=">pNL_oligo_552"
     primer_bind     4618..4635
                     /label="M13F(-21)"
     primer_bind     4619..4634
                     /label=">pNL_oligo_37"
     primer_bind     4619..4635
                     /label="M13 fwd"
     primer_bind     4628..4653
                     /label=">pNL_oligo_1548"
     primer_bind     4642..4660
                     /label=">pNL_oligo_3104"
     promoter        4642..4660
                     /label="T7 promoter"
     primer_bind     4642..4661
                     /label="T7"
     primer_bind     4643..4659
                     /label=">pNL_oligo_16"
     misc_feature    complement(4648..4661)
                     /label=">pNL_oligo_1583"
     misc_feature    complement(4648..4661)
                     /label=">pNL_oligo_2086"
     misc_feature    4669..5668
                     /label="promLBD16"
     misc_feature    5670..5670
                     /label="GG1"
     CDS             5673..7508
                     /label="PhiC31plant-NLS stop without start CDS"
     misc_feature    7513..8012
                     /label="tUBQ1"
ORIGIN
        1 accacagaat caggggataa cgcaggaaag aacatgaagg ccttgacagg atatattggc
       61 gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa
      121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaagaag agttggtagc
      661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
      781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
      841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgtg
      901 taacattggt ctagtgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
      961 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     1021 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     1081 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     1141 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     1201 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     1261 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     1321 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     1381 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg gatcgcagtg
     1441 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     1501 aattccgtca gccagtttag tctgaccatc tcatctgtaa caacattggc aacgctacct
     1561 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg gtagattgtc
     1621 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     1681 ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct cataacaccc
     1741 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     1801 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     1861 tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca
     1921 aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc
     1981 tccctcactt tctggctgga tgatggggcg attcaggcga tccccatcca acagcccgcc
     2041 gtcgagcggg cttttttatc cccggaagcc tgtggataga gggtagttat ccacgtgaaa
     2101 ccgctaatgc cccgcaaagc cttgattcac ggggctttcc ggcccgctcc aaaaactatc
     2161 cacgtgaaat cgctaatcag ggtacgtgaa atcgctaatc ggagtacgtg aaatcgctaa
     2221 taaggtcacg tgaaatcgct aatcaaaaag gcacgtgaga acgctaatag ccctttcaga
     2281 tcaacagctt gcaaacaccc ctcgctccgg caagtagtta cagcaagtag tatgttcaat
     2341 tagcttttca attatgaata tatatatcaa ttattggtcg cccttggctt gtggacaatg
     2401 cgctacgcgc accggctccg cccgtggaca accgcaagcg gttgcccacc gtcgagcgcc
     2461 agcgcctttg cccacaaccc ggcggccggc cgcaacagat cgttttataa attttttttt
     2521 ttgaaaaaga aaaagcccga aaggcggcaa cctctcgggc ttctggattt ccgatccccg
     2581 gaattagaga tcttggcagg atatattgtg gtgtaacgtt atcgatctgg attttagtac
     2641 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
     2701 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctataaga accctaattt
     2761 cccttatcgg gaaactactc acacattatt tatggagaaa atagagagag atagatttgt
     2821 agagagagac tggtgatttc agcgtaccga attaattctc ccgcgaccag ccgagcgagc
     2881 ttagcgaact gtggacgaga actgtgccac caagcgtaag gccgttctct cgcatttgcc
     2941 ttgctaggct cgcgcgagtt gctggctgag gcgttctcga aatcagctct tgttcggtcg
     3001 gcatctactc tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc
     3061 gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg
     3121 cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc
     3181 atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat
     3241 atacgcccgg aggcgtggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt
     3301 ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg
     3361 ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt
     3421 caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc
     3481 ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat
     3541 cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt
     3601 agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc
     3661 ggccgcagcg atcgcatcca tagcctccgc gaccggctga agaacagcgg gcagttcggt
     3721 ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct
     3781 ctcgctgaac tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg
     3841 ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata
     3901 tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat
     3961 caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag
     4021 ttcaggcttt ttcataggga tcagcttggg ctgtcctctc caaatgaaat gaacttcctt
     4081 atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
     4141 agatgtcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
     4201 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaatga
     4261 tagcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt tctactgtcc
     4321 tttcgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gaaattaccc
     4381 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg acatttttgg
     4441 agtaggggta cgataacatt aacgtttaca atttcgcgcc attcgccatt caggctgcgc
     4501 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     4561 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     4621 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcttggagCC ATTATTGATT
     4681 GTCTCCTTAG AATCCAACAT GTGACCCTCC ATTGTCTACC ATTCACACCC TTTGGCTCAC
     4741 TCGATAAATT TGGACATTAA TTGCATTCAT TTTATTTGTA AGAACCAGAA TCTCAGTAGA
     4801 TGTTATTTGT GTTGAGTAAA CTGTTGTTAT TTATAGACTA GGCTATTCTT CGTATACACT
     4861 TTGATACTGA TTTTTAGTTC ATCATCTATC TACCGACTAA ATATAGTGTT TCTATGTGGC
     4921 TTCTCCTCAA ATGTCAAATT TATTTCTTTA ATGTTGTTTG AGTTTCAAAC TTGCAAAGTG
     4981 CAAACCAATT TTGGTTGTTA TACTAAAACC ACACATATAA TTAAAGACAC CCACTATAGC
     5041 ATAAAATTCG TCGTGTAAGC AATTTTACTA TAGTCTAAAC TCCATAAAAG GACGTCGAAA
     5101 AATATTGTGA TATTGGAATT ATAGTGTCAG TTTGTTTTTC TTTGGGCAAA TGGATTGTAG
     5161 TTTCATTATA TATTACAATT TATAAACGAA ACGATAAGTA TGGCTCATAC TTTAGTGATG
     5221 GAGAATTAAA GTATTTTATA TTCAGAGATG ACACAATATG GTAGACCAAA AATAACTAAT
     5281 TAAAATAGTA TCATATTAGA TATTTATATG TGATTGTAAA TTTGATCATT GAGTGAACAT
     5341 TTTAAATTAG TATGCCCAAG AAAGAAAAGA AAAAAAAAAA ACTCTTTCTC TCTCATGATA
     5401 ATGTTCCTTT TATAAAATCC ACCCAAAGAC ACCAAACACT TCTCCATCAT CATTTCATTT
     5461 CTTGTTCTTT TTCCACCATC ACCATTTCCT AGTTCGCTCG TGCACATCTC CGACGATCCC
     5521 AATTCTAAAA TCATCACTTC TTCCAATCTT TTCAACATCT CTTCAAAATA TCCAGTCAAC
     5581 AAAAAAAAAA GTACTTAAAA CAAGGAAACC CCTTCTTTCT TTTTGTACAT ATAACATAAA
     5641 AATCATCACT TTTTTGTTCG TTTCGCCGAa tggacacgta tgccggcgct tatgatagac
     5701 aatcaagaga aagagagaac tcttctgccg cctctccagc tacacagaga tctgccaacg
     5761 aggataaagc tgctgattta caaagagagg tagagagaga tgggggtagg tttagatttg
     5821 tgggtcattt ctctgaggct cctggtacat cagcctttgg tactgctgaa cggcctgaat
     5881 ttgaaagaat attaaatgag tgtcgggccg gtcgactaaa tatgataata gtttatgatg
     5941 tatctaggtt cagtagactc aaagttatgg atgcaattcc gatcgtgagc gagctacttg
     6001 cactcggtgt tactatcgta agtacacaag aaggtgtatt caggcagggt aatgtgatgg
     6061 accttataca ccttattatg cgactcgacg ctagccataa agagtcatca ctgaagagtg
     6121 ctaaaatctt ggacaccaag aacctgcaaa gggagcttgg aggctatgta ggaggaaaag
     6181 ctccgtatgg tttcgaactc gtgagtgaga caaaagagat taccagaaat ggtaggatgg
     6241 tgaacgtggt tattaataag ctagctcata gcacaacccc tctcacaggg ccttttgagt
     6301 tcgaacctga cgttattaga tggtggtgga gagagatcaa aactcacaaa catcttccat
     6361 ttaagccagg tagccaggct gctatccacc ctggatctat cactgggctt tgtaaaagga
     6421 tggatgctga cgcagtgcca actcgagggg aaacaatcgg taagaagact gcatcttccg
     6481 cttgggatcc cgctacagtt atgagaatcc ttcgggatcc gcgtattgct ggattcgccg
     6541 cagaagtcat ttataagaaa aaacctgatg gtactcctac tacaaaaatc gaaggttata
     6601 ggatccaaag ggatcctatt actttgcgtc cagttgaatt agactgtgga ccgatcatcg
     6661 aacctgccga gtggtacgag ttgcaggcct ggcttgatgg tagagggcgt ggaaagggtt
     6721 tatctcgggg acaggcaatt ttgtcagcaa tggataagct ttattgtgag tgtggggctg
     6781 ttatgacatc aaaacgtgga gaagagtcaa tcaaggattc ttacaggtgc agacgtcgta
     6841 aagtggtgga cccttctgct ccaggacagc atgagggtac atgcaacgtt tctatggctg
     6901 ctcttgataa atttgtggcc gaacgtattt tcaacaagat tagacacgct gaaggggatg
     6961 aggaaacttt agctcttctt tgggaggctg cccggcgttt tggtaagcta acagaggcac
     7021 cagaaaaaag tggcgaaaga gctaatttgg tagccgaaag agcagacgcc ttgaatgcat
     7081 tggaagaact ttacgaagat agggctgccg gagcatatga tggaccagtt ggtcggaagc
     7141 atttcaggaa gcaacaagct gcactaactt tgagacagca aggcgcagag gagcgactcg
     7201 cagagcttga ggccgccgag gccccaaagc ttccactcga tcaatggttt cctgaggatg
     7261 ccgacgctga tcctacaggt ccaaagagct ggtggggaag agctagcgta gacgataaga
     7321 gagtgtttgt gggtctattt gttgacaaga ttgtagttac taagtccact acgggtcgag
     7381 gacagggcac tcccatcgaa aagagagcca gtattacctg ggcaaagcct ccaaccgatg
     7441 atgatgaaga cgatgcacaa gatggtaccg aggatgtggc agcaccaaaa aaaaagagga
     7501 aggtgtaagc ttagagactc ttatcaagaa tcccatctct tgcttgcttt tttttgttgt
     7561 cttccctttg atagggtttg tttttcttgt ttcagtgact ttctatgtta aaagataatg
     7621 tcagtaaaag gatttggttt tctattattc tgaatcgatt acggaagatt cttgcttaat
     7681 tccaatctat acaagtatcg tgaaataatg accgtttatg tgattaggag acgtgtttca
     7741 ttaataaaat ataagatcaa tacattgtta gtagtgataa actatgtaca aattgtattg
     7801 attgtaaaag aaacacaata ggttcctttt ttctacaata tattgtgaca gactctctgt
     7861 tttaacgaat gaattaaatt tgtcaacgaa aaaattaaag aatgatgtga tcactttgca
     7921 ccttggttca tcttcagagg cttctgcatg ttccatgcat tgaggctctc tacggtcaca
     7981 ggttgtgagc cgttgttaaa gacaaacaaa tgcgct
//