LOCUS       T1T_pUBQ10_mTurq        9124 bp ds-DNA     circular     21-MAR-2023
DEFINITION  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(4197..4843)
                     /locus_tag="rbcS terminator_48839"
                     /label="rbcS terminator_48839"
                     /ApEinfo_label="rbcS terminator_48839"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(4844..5578)
                     /locus_tag="VP40-mScarlet CDS"
                     /label="VP40-mScarlet CDS"
                     /ApEinfo_label="VP40-mScarlet CDS"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          5535..5554
                     /note="sequence: ctcacaccactaccacttcc"
                     /locus_tag="5600"
                     /label="5600"
                     /ApEinfo_label="5600"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5579..5584)
                     /locus_tag="Short enhancer for gene expression"
                     /label="Short enhancer for gene expression"
                     /ApEinfo_label="Short enhancer for gene expression"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5589..5638
                     /locus_tag="attPTp901"
                     /label="attPTp901"
                     /ApEinfo_label="attPTp901"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          5612..5642
                     /note="sequence: CAATTAAGGTAACTAAAAAACTCCTTTggag"
                     /locus_tag="attPTp901_fwd"
                     /label="attPTp901_fwd"
                     /ApEinfo_label="attPTp901_fwd"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5639..5642
                     /locus_tag="GG0"
                     /label="GG0"
                     /ApEinfo_label="GG0"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5643..7668
                     /locus_tag="pUBQ10"
                     /label="pUBQ10"
                     /ApEinfo_label="pUBQ10"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          complement(6114..6135)
                     /note="sequence: gggttaagtgcaactttgatgg"
                     /locus_tag="pUBQ10_rev"
                     /label="pUBQ10_rev"
                     /ApEinfo_label="pUBQ10_rev"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          7340..7364
                     /note="sequence: ctcaattctctctaccgtgatcaag"
                     /locus_tag="5595"
                     /label="5595"
                     /ApEinfo_label="5595"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7669..7672
                     /locus_tag="GG"
                     /label="GG"
                     /ApEinfo_label="GG"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(7673..7725)
                     /locus_tag="attB Tp901"
                     /label="attB Tp901"
                     /ApEinfo_label="attB Tp901"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7726..7728
                     /locus_tag="GG2"
                     /label="GG2"
                     /ApEinfo_label="GG2"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7735..7773
                     /locus_tag="SV40 plus linker"
                     /label="SV40 plus linker"
                     /ApEinfo_label="SV40 plus linker"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7774..8490
                     /locus_tag="mTurquoise from PIN sequence CDS"
                     /label="mTurquoise from PIN sequence CDS"
                     /ApEinfo_label="mTurquoise from PIN sequence CDS"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer          complement(7887..7904)
                     /note="sequence: gtcagcttgccgtaggtg"
                     /locus_tag="5596"
                     /label="5596"
                     /ApEinfo_label="5596"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8491..8496
                     /locus_tag="Stop codon"
                     /label="Stop codon"
                     /ApEinfo_label="Stop codon"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8497..9124
                     /locus_tag="UBQ10 terminator_48841"
                     /label="UBQ10 terminator_48841"
                     /ApEinfo_label="UBQ10 terminator_48841"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1..4196
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /locus_tag="source:synthetic DNA construct"
                     /label="source:synthetic DNA construct"
                     /ApEinfo_label="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    82..106
                     /note="right border repeat from nopaline C58 T-DNA"
                     /locus_tag="RB T-DNA repeat"
                     /label="RB T-DNA repeat"
                     /ApEinfo_label="RB T-DNA repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      257..692
                     /note="origin of replication from bacterial plasmid pSa"
                     /locus_tag="pSa ori"
                     /label="pSa ori"
                     /ApEinfo_label="pSa ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(972..991)
                     /note="pENTR vectors, reverse primer"
                     /locus_tag="pENTR-R"
                     /label="pENTR-R"
                     /ApEinfo_label="pENTR-R"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             983..1798
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to kanamycin in bacteria or G418
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     G
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     K
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     A
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     I ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1056..1075)
                     /note="Kanamycin resistance gene, reverse primer"
                     /locus_tag="Kan-R"
                     /label="Kan-R"
                     /ApEinfo_label="Kan-R"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1899..2487
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2388..2407
                     /note="pBR322 origin, forward primer"
                     /locus_tag="pBR322ori-F"
                     /label="pBR322ori-F"
                     /ApEinfo_label="pBR322ori-F"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2554..2576
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
                     /locus_tag="LB T-DNA repeat"
                     /label="LB T-DNA repeat"
                     /ApEinfo_label="LB T-DNA repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2600..2852
                     /note="nopaline synthase terminator and poly(A) signal"
                     /locus_tag="NOS terminator"
                     /label="NOS terminator"
                     /ApEinfo_label="NOS terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2892..3686)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     P
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     S
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     Q
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     A LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /locus_tag="NeoR/KanR"
                     /label="NeoR/KanR"
                     /ApEinfo_label="NeoR/KanR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3003..3022)
                     /note="Neomycin resistance gene, forward primer"
                     /locus_tag="Neo-F"
                     /label="Neo-F"
                     /ApEinfo_label="Neo-F"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3613..3632
                     /note="Neomycin resistance gene, reverse primer"
                     /locus_tag="Neo-R"
                     /label="Neo-R"
                     /ApEinfo_label="Neo-R"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(3720..3899)
                     /note="nopaline synthase promoter"
                     /locus_tag="NOS promoter"
                     /label="NOS promoter"
                     /ApEinfo_label="NOS promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4131..4153
                     /note="In lacZ gene"
                     /locus_tag="M13/pUC Forward"
                     /label="M13/pUC Forward"
                     /ApEinfo_label="M13/pUC Forward"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4145..4162
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
                     /locus_tag="M13 Forward"
                     /label="M13 Forward"
                     /ApEinfo_label="M13 Forward"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4146..4162
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 fwd"
                     /label="M13 fwd"
                     /ApEinfo_label="M13 fwd"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4172..4191
                     /note="T7 promoter, forward primer"
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        4172..4190
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 aagtaaattg atgatcacaa gtgaaagtgt atgttgctgc cacagaatca ggggataacg
       61 caggaaagaa catgaaggcc ttgacaggat atattggcgg gtaaactaag tcgctgtatg
      121 tgtttgtttg agatctaatt ccggggatcg gaaatccaga agcccgagag gttgccgcct
      181 ttcgggcttt ttctttttca aaaaaaaaaa tttataaaac gatctgttgc ggccggccgc
      241 cgggttgtgg gcaaaggcgc tcgacggtgg gcaaccgctt gcggttgtcc acgggcggag
      301 ccggtgcgcg tagcgcattg tccacaagcc aagggcgacc aataattgat atatatattc
      361 ataattgaaa agctaattga acatactact tgctgtaact acttgccgga gcgaggggtg
      421 tttgcaagct gttgatctga aagggctatt agcgttctca cgtgcctttt tgattagcga
      481 tttcacgtga ccttattagc gatttcacgt actccgatta gcgatttcac gtaccctgat
      541 tagcgatttc acgtggatag tttttggagc gggccggaaa gccccgtgaa tcaaggcttt
      601 gcggggcatt agcggtttca cgtggataac taccctctat ccacaggctt ccggggataa
      661 aaaagcccgc tcgacggcgg gctgttggat ggggatcgcc tgaatcgccc catcatccag
      721 ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat
      781 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc
      841 cttcaactca gcaaaagttc gatttattca acaaagccac gttgtggctc aaaatctctg
      901 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
      961 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcaaggcc
     1021 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     1081 cgggcaatca ggtgcgacaa tctaccgatt gtatgggaag cccgatgcgc cagagttgtt
     1141 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     1201 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     1261 tgcatggtta ctcaccactg cgatcccagg gaaaacagca ttccaggtat tagaagaata
     1321 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     1381 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     1441 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     1501 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     1561 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     1621 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     1681 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     1741 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     1801 agaattggtt aattggttgt aaccatgacc aaaatccctt aacgtgagtt ttcgttccac
     1861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2041 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2281 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2521 gccttttgct ggccttttgc tcacatgaga tcttggcagg atatattgtg gtgtaacgtt
     2581 atcagcttgc atgccggtcg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     2641 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     2701 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     2761 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     2821 aagaaacttt attgccaaat gtttgaacga tctgcttgac tctagctaga gtccgaaccc
     2881 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     2941 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     3001 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     3061 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     3121 ccgtgggtca cgacgagatc ctcgccgtcg ggcatccgcg ccttgagcct ggcgaacagt
     3181 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     3241 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     3301 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     3361 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     3421 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     3481 cacgatagcc gcgctgcctc gtcttggagt tcattcaggg caccggacag gtcggtcttg
     3541 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     3601 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     3661 gcgtgcaatc catcttgttc aatcatgcct cgatcgagtt gagagtgaat atgagactct
     3721 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     3781 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     3841 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     3901 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     3961 ccttaattct ccgctcatga tcgataacat taacgcttac aatttccatt cgccattcag
     4021 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     4081 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     4141 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcggagcgat
     4201 tgatgcatgt tgtcaatcaa ttggcaagtc ataaaatgca ttaaaaaata ttttcatact
     4261 caactacaaa tccatgagta taactataat tataaagcaa tgattagaat ctgacaagga
     4321 ttctggaaaa ttacataaag gaaagttcat aaatgtctaa aacacaagag gacatacttg
     4381 tattcagtaa catttgcagc ttttctaggt ctgaaaatat atttgttgcc tagtgaataa
     4441 gcataatggt acaactacaa gtgttttact cctcatatta acttcggtca ttagaggcca
     4501 cgatttgaca catttttact caaaacaaaa tgtttgcata tctcttataa tttcaaattc
     4561 aacacacaac aaataagaga aaaaacaaat aatattaatt tgagaatgaa caaaaggacc
     4621 atatcattca ttaactcttc tccatccatt tccatttcac agttcgatag cgaaaaccga
     4681 ataaaaaaca cagtaaatta caagcacaac aaatggtaca agaaaaacag ttttcccaat
     4741 gccataatac tcaaactcag taggattctg gtgtgtgcgc aatgaaactg atgcattgaa
     4801 cttgacgaac gttgtcgaaa ccgatgatac gaacgaaagc tttttacttg tacagctcgt
     4861 ccatgccgcc ggtggagtgg cggccctcgg agcgttcgta ctgttccacc acggtgtagt
     4921 cctcgttgtg ggaggtgatg tccaacttgc ggtcgacgtt gtaggcgccg ggcatctgca
     4981 cgggcttctt ggccttgtag gtggtcttga agtccgccag gtagcggccg ccgtccttca
     5041 ggcgcagggc catcttaatg tcgcccttca gcacgccgtc ctcggggtac aaccgctcgg
     5101 tggacgcttc ccagcccatt gtcttcttct gcattacggg gccgtcagga gggaagttgg
     5161 tgccgcggag cttcaccttg tagatcaggg tgccgtcctc cagggaggtg tcctgggtca
     5221 cggtcacggc gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
     5281 aggactgctt atagtagtcg gggatgtcgg cggggtgctt ggtgaaggcc ctggagccgt
     5341 acatgaactg aggggacagg atgtcccagg agaagggcag ggggccaccc ttggtcacct
     5401 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
     5461 actcgtggcc gttcatggag ccctccatgt gcaccttgaa ccgcatgaac tccttgatca
     5521 ctgcctcgcc cttgctcaca ccactaccac ttccgacttt cctcttcttc tttggcattg
     5581 tttactatGC GAGTTTTTAT TTCGTTTATT TCAATTAAGG TAACTAAAAA ACTCCTTTgg
     5641 agctcaacaa caaactttcc attcacccat ttatggatac acagtctagc tcaacagagc
     5701 ttttaaccca aattggtaca atagaataca actttagatc ataattctca aaagaaagag
     5761 attccttagc tattctatct gccactccat ttccttctcg gcttgtatgc acaagcataa
     5821 aatcctcaaa cttgctaagt agatacttta tgtcttggat aattggattg agacttgaca
     5881 agcataactt tcatgtaacc aaagacacaa gttgctgaga atccacctca aaaatgatct
     5941 tcctataatt gaatcgggat aatgacagca cagcccatct aagagcctcc acttctactt
     6001 ccagcacgct tcttactttt accacagctc ttgcacctaa ccataacacc ttccctgtat
     6061 gatcgcgaag cacccaccct aagccacatt ttaatccttc tgttggccat gccccatcaa
     6121 agttgcactt aacccaagat tgtggtggag cttcccatgt ttctcgtctg tcccgacggt
     6181 gttgtggttg gtgctttcct tacattctga gcctctttcc ttctaatcca ctcatctgca
     6241 tcttcttgtg tccttactaa tacctcattg gttccaaatt ccctcccttt aagcaccagc
     6301 tcgtttctgt tcttccacag cctcccaagt atccaaggga ctaaagcctc cacattcttc
     6361 agatcaggat attcttgttt aagatgttga actctatgga ggtttgtatg aactgatgat
     6421 ctaggaccgg ataagttccc ttcttcatag cgaacttatt caaagaatgt tttgtgtatc
     6481 attcttgtta cattgttatt aatgaaaaaa tattattggt cattggactg aacacgagtg
     6541 ttaaatatgg accaggcccc aaataagatc cattgatata tgaattaaat aacaagaata
     6601 aatcgagtca ccaaaccact tgcctttttt aacgagactt gttcaccaac ttgatacaaa
     6661 agtcattatc ctatgcaaat caataatcat acaaaaatat ccaataacac taaaaaatta
     6721 aaagaaatgg ataatttcac aatatgttat acgataaaga agttactttt ccaagaaatt
     6781 cactgatttt ataagcccac ttgcattaga taaatggcaa aaaaaaacaa aaaggaaaag
     6841 aaataaagca cgaagaattc tagaaaatac gaaatacgct tcaatgcagt gggacccacg
     6901 gttcaattat tgccaatttt cagctccacc gtatatttaa aaaataaaac gataatgcta
     6961 aaaaaatata aatcgtaacg atcgttaaat ctcaacggct ggatcttatg acgaccgtta
     7021 gaaattgtgg ttgtcgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca
     7081 cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca
     7141 attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta
     7201 gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct
     7261 tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc
     7321 aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc
     7381 cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt
     7441 tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat
     7501 catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt
     7561 tttgtcgaat aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag
     7621 tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacagTA CTGCAAAAAA
     7681 AGCAAAAAGC ATTTACCTTG ATTGAGATGT TAATTGTGTT GGCATaccta aacaatgcca
     7741 aagaagaaga ggaaagtcgg aagtggtagt ggtatggtga gcaagggcga ggagctgttc
     7801 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     7861 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     7921 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgtc ctggggcgtg
     7981 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     8041 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     8101 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     8161 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta catcagcgac
     8221 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     8281 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     8341 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
     8401 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     8461 atcactctcg gcatggacga gctgtacaag taataaatct cgtctctgtt atgcttaaga
     8521 agttcaatgt ttcgtttcat gtaaaacttt ggtggtttgt gttttggggc cttgtataat
     8581 ccctgatgaa taagtgttct actatgtttc cgttcctgtt atctctttct ttctaatgac
     8641 aagtcgaact tcttctttat catcgcttcg tttttattat ctgtgcttct tttgtttaat
     8701 acgcctgcaa agtgactcga ctctgtttag tgcagttctg cgaaacttgt aaatagtcca
     8761 attgttggcc tctagtaata gatgtagcga aagtgttgag ctgttgggtt ctaaggatgg
     8821 cttgaacatg ttaatctttt aggttctgag tatgatgaac attcgttgtt gctaagaaat
     8881 gcctgtaatg tcccacaaat gtagaaaatg gttcgtacct ttgtccaagc attgatatgt
     8941 ctgatgagag gaaactgcaa gatactgagc ttggtttaac gaaggagagg cagtttcttc
     9001 cttccaaagc atttcatttg acaatgcctt gatcatctta agtagagttt ctgttgtgga
     9061 aagtttgaaa ctttgaagaa acgactctca agtaaattga tgatcacaag tgaaagtgta
     9121 tgtt     
//
