LOCUS       Exported                5207 bp ds-DNA     circular SYN 23-JUN-2008
DEFINITION  pENTR5 TOPO Sequence sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     See Addgene Vectorpedia pENTR5 TOPO Sequence sequence at 
            www.addgene.org
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13_forward20_primer
     misc_recomb     complement(56..139)
                     /label=attL4
                     /note="/vntifkey=21"
     primer_bind     170..205
                     /label=desmin MCS fw
     promoter        188..2690
                     /label=desmin promoter region
     5'UTR           2647..2690
                     /label=desmin 5' UTR
     primer_bind     complement(2672..2696)
                     /label=desmin MCS rev
     misc_recomb     complement(2736..2819)
                     /label=attR1
                     /note="/vntifkey=21"
     promoter        complement(2913..2931)
                     /label=T7_promoter
                     /note="/vntifkey=30"
     misc_feature    complement(2932..2950)
                     /label=M13_reverse_primer
                     /note="/vntifkey=21"
     CDS             3065..3874
                     /codon_start=1
                     /gene="KanR2"
                     /label=KanR2
                     /note="Orf frame 3"
                     /note="/vntifkey=4"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4005..4624
                     /label=pBR322_origin
                     /note="/vntifkey=33"
     terminator      complement(4939..4966)
                     /label=rrnB_T2_terminator
                     /note="/vntifkey=43"
     terminator      complement(5098..5141)
                     /label=rrnB_T1_terminator
                     /note="/vntifkey=43"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc ccgagttaac gctaccatgg agctccaaat
       61 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
      121 ttttataatg ccaactttgt atagaaaagt tggctccgaa ttcgcccttg atatcactga
      181 tgctagctcc ttgaggcact ttcggtgtcc ttacaaacac agatcccata cattacattc
      241 acagatacac aaaagattta acaccttatc aatttatatt gtgtataaac acttcttaat
      301 aaagtgtttt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtttctgt
      361 ctgtctgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtct gtgtgtgtga
      421 tagtcgagtc tctggcctat caagactcca ttatttttcc aggaggcctt caggacagta
      481 gactggtgat ggggttcaga cgggaagaat attaatagcc atgacagcag cagctgtgga
      541 gcaacatcac agcttctcgg ttactatggt cagcctgagg aatgggccgg actgatgggg
      601 gttgagcgac agggatctta tttgggatcg catttatccg atgacacact tacattaatc
      661 ggatttccca tgtgtaatgt aagagacaaa ctaattaaga atattccaca tgtgaaaaaa
      721 tgatgtggta ctactaaaat attaggcctt actcatatgg tggtgcaata ttttgtatgg
      781 gaggagatgc aagtcacaca gcagtgcttg atttctgttt caacatccta gtacattttt
      841 tttaatcata attcacatat tttaatgcaa ccatatgcac attactgatg tgatatgtcc
      901 aaaaaaacaa acaaactgca aattagtagc acaacagaca cttagagtaa gccaggggca
      961 gaccaggact aaaaatcagc cctggcactg tagccacatc agcccacatt atcagaccga
     1021 cacagcccca ccctcggaca tgcatattca ctacttttat tggtgtacag atggtaaaac
     1081 aatataagca gtaccatatg taatagatat ttaatagata tttattttgt gtttggactt
     1141 gtgagggaaa ccaggaagaa acccacacga acatgcaaac tccaaaatga aacacctaat
     1201 gacccagccg aggctcgaac cattgacctt tttgctgtga ggcggcagcg ctacccattg
     1261 tgctgccccc ttttgagact atttcagtta attttatgca tttggcagcg tctgatttta
     1321 gagcaatttt tatgtttctc tgagcttttt ggcaccgcct ttcctttttt ttacagtcca
     1381 tctctgacaa ttagcttgtg ttgcacctaa tgtttatttg acattcttgc cagattttga
     1441 ttatgtccgc ataacctctg attgggttgg cccatctcga aaccagctgg ccattgccga
     1501 ttgggccaat gcttaacatt tattattatt actattatca tcatcattat catcatcatc
     1561 atcatcatca tcatcatcat catcatcatc atcataatat tcacatcttg caagaaacaa
     1621 aaactgcctg tatgtaagca aaaaataaaa aaaaattata aaaaacataa cataaaacat
     1681 aaaaagtatg taaaaaaaat aataaaaaat agcatacccg cccacattta aaaaatggtc
     1741 cagccctttt ggcatttgcc agaattgcca gatggccagt ccgcccctgg actaggcaac
     1801 cctgatgcca atatgcttat ttcacgcggt cgccatttta aagaaccaaa gcgaaggctg
     1861 cggtgggaag aaagctggaa ttataggacc agcactgtaa acattgcagt aaaatgctgt
     1921 ggactacaaa cctccagctg ttgccgagat ttggaggttt gtagtccaca gcatgttact
     1981 gcaatgtata cagtgctggt cctatactta tatatatata tatactgaag aaagatggaa
     2041 acttggaact tggtaaaaca atgtaaacta atgtaaactt tccagcattc ttcaaaatat
     2101 catttttgat gttcaacaga agaaaaaaaa aggttttaaa caacttgagg gtgactaaat
     2161 tgtgaattaa ttttcatttt tgggtgaact atccctttag gataggaatt agataacttt
     2221 ttcaaacttt gtagtccttt tatattcata aaaatctaaa aagaaacaat attaaatgaa
     2281 atatttatga cagctcaatg tggccacttt taatagaaaa cccttttgct ttttcaaaca
     2341 cacacacaaa ctcatcagtc taatatggtg tttattgggc cacaagcact cttcctggta
     2401 agcagaagtg acagcacagt ttggcatggc gtgacctgtg ccacctcatg ccttgtccag
     2461 ttgctccacc cagctgctgg aaaccagacc tcatgacagc tgaggaagca aggtttagaa
     2521 aagagagagg gagagataga agtgggcgcc aatgtgtgtg ggttgagaac agtgactaaa
     2581 aaaatgtggg tgggggcaaa cgggagtggt ctctatatat cccctcttct gctctgctta
     2641 accctcattc ttccacacag tcctcagaga cccagcattc acacagcgta gtcgacaagg
     2701 gcgaattcga cccaagtttg tacaaaaaag ttgaacgaga aacgtaaaat gatataaata
     2761 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     2821 atatgcagtc actatgaacc aactacttag atggtattag tgacctgtag aattaattcg
     2881 agctctagag ctgcagggcg gccgcgatat cccctatagt gagtcgtatt acatggtcat
     2941 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3001 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     3061 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     3121 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     3181 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     3241 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     3301 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     3361 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     3421 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     3481 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     3541 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     3601 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     3661 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     3721 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     3781 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     3841 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     3901 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     3961 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4021 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4081 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4141 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4201 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4261 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4321 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4381 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4441 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4501 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4561 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4621 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4681 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4741 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4801 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4861 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
     4921 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
     4981 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     5041 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     5101 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     5161 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgtt
//
