LOCUS       Exported                3735 bp ds-DNA     circular SYN 23-JUN-2008
DEFINITION  pENTR5 TOPO Sequence sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     See Addgene Vectorpedia pENTR5 TOPO Sequence sequence at 
            www.addgene.org
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13_forward20_primer
     misc_recomb     complement(56..139)
                     /label=attL4
                     /note="/vntifkey=21"
     promoter        170..1224
                     /label=exorh
     primer_bind     170..189
                     /label=exorh fw
     primer_bind     complement(1205..1224)
                     /label=exorh plasmid rev
     misc_feature    1226
                     /label=TOPO Insert
                     /label=nonstandard type:  insertion_seq
                     /note="Insertion site between two As."
                     /note="/vntifkey=14"
     misc_recomb     complement(1264..1347)
                     /label=attR1
                     /note="/vntifkey=21"
     promoter        complement(1441..1459)
                     /label=T7_promoter
                     /note="/vntifkey=30"
     primer_bind     complement(1460..1478)
                     /label=M13_reverse_primer
     CDS             1593..2402
                     /codon_start=1
                     /gene="KanR2"
                     /label=KanR2
                     /note="Orf frame 3"
                     /note="/vntifkey=4"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2533..3152
                     /label=pBR322_origin
                     /note="/vntifkey=33"
     terminator      complement(3467..3494)
                     /label=rrnB_T2_terminator
                     /note="/vntifkey=43"
     terminator      complement(3626..3669)
                     /label=rrnB_T1_terminator
                     /note="/vntifkey=43"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc ccgagttaac gctaccatgg agctccaaat
       61 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
      121 ttttataatg ccaactttgt atagaaaagt tggctccgaa ttcgcccttg ctcagctggc
      181 agtactaccg ctcactcctg cccgacgtca gcatcaccat gtgcccatcc tgcttccagg
      241 tgcgctcatc tgtgtgtgtg tgtgtgtgtg tctcctctgt tatttgtctg tttgactcta
      301 tatgctttgt tgtgtgtctg atggttgtgt gtgtgtgtgt gtgtgtgtgt ttctcagatg
      361 ttccacagtg aggactatga gctgttggtt cttcagcaca gctgctgccc gttctgccgc
      421 agacccatcg atgagtcctg ctaacacaca ctcatgttta ccgcggtgtg atcatcagtc
      481 ctgctaacac acactcatgt ttaccgcggt gtgatccacc ctgagccgct gtaaagccgt
      541 cagtaaatgc cagctcagca cagctctgat aggattacac agatcaacac aaagcagatt
      601 gcagcgatgc tggacacaca cacacacaca cacacacaca ctcacagctc aacacactcc
      661 tgcgcatcca cacatccctc atgttggtgt ttttaataaa gatttaatat ttcagtctga
      721 taatctgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
      781 gtgtgtgtgt gagaccgtga agtgtccctt gtgtttggtc ttcatcttat gaggctcaca
      841 caggacttgc tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
      901 ttgtccagca ggagggttgt gttctgcagg acacctgtct gtctgtggat gagagatttc
      961 agctctgtgc aggagtgttt ggatggagac gcagcctgca cgcgctgatg agcagcagta
     1021 gtgtgtgtgt gtgtgtcgtg agtgtgtgtg gtgagagcga gcgctgctgt gtctccatct
     1081 ctggattatc cccctgtctg accccaatct gaccactcac tcctgtaatc tgcctaatct
     1141 ggactcatgg ggttcacata agagcgcagc gccggctctc cgggcttcat ctgcggaggt
     1201 gtgccgatcg tccacttctc catcaagggc gaattcgacc caagtttgta caaaaaagtt
     1261 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     1321 cagactacat aatactgtaa aacacaacat atgcagtcac tatgaaccaa ctacttagat
     1381 ggtattagtg acctgtagaa ttaattcgag ctctagagct gcagggcggc cgcgatatcc
     1441 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1501 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     1561 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     1621 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1681 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     1741 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     1801 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     1861 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     1921 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     1981 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     2041 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     2101 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     2161 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2221 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2281 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2341 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     2401 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     2461 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2521 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2581 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2641 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2701 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2761 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2821 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2881 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2941 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3001 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3061 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3121 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3181 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3241 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3301 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3361 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3421 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3481 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3541 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     3601 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     3661 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
     3721 cccagtcacg acgtt
//
