LOCUS       Exported                4466 bp ds-DNA     circular SYN 23-JUN-2008
DEFINITION  pENTR5 TOPO Sequence sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     See Addgene Vectorpedia pENTR5 TOPO Sequence sequence at 
            www.addgene.org
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13_forward20_primer
     misc_recomb     complement(56..139)
                     /label=attL4
                     /note="/vntifkey=21"
     primer_bind     170..201
                     /label=ASP7181-exorh+GFP fw
     promoter        182..1236
                     /label=exorh
     CDS             1237..1947
                     /codon_start=1
                     /label=mCerulean
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDEL"
     primer_bind     complement(1928..1955)
                     /label=mcerulean-STOP-Asp7181 rv
     misc_feature    1957
                     /label=TOPO Insert
                     /label=nonstandard type:  insertion_seq
                     /note="Insertion site between two As."
                     /note="/vntifkey=14"
     misc_recomb     complement(1995..2078)
                     /label=attR1
                     /note="/vntifkey=21"
     promoter        complement(2172..2190)
                     /label=T7_promoter
                     /note="/vntifkey=30"
     primer_bind     complement(2191..2209)
                     /label=M13_reverse_primer
     CDS             2324..3133
                     /codon_start=1
                     /gene="KanR2"
                     /label=KanR2
                     /note="Orf frame 3"
                     /note="/vntifkey=4"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3264..3883
                     /label=pBR322_origin
                     /note="/vntifkey=33"
     terminator      complement(4198..4225)
                     /label=rrnB_T2_terminator
                     /note="/vntifkey=43"
     terminator      complement(4357..4400)
                     /label=rrnB_T1_terminator
                     /note="/vntifkey=43"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc ccgagttaac gctaccatgg agctccaaat
       61 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
      121 ttttataatg ccaactttgt atagaaaagt tggctccgaa ttcgccctta gatctggtac
      181 cgctcagctg gcagtactac cgctcactcc tgcccgacgt cagcatcacc atgtgcccat
      241 cctgcttcca ggtgcgctca tctgtgtgtg tgtgtgtgtg tgtctcctct gttatttgtc
      301 tgtttgactc tatatgcttt gttgtgtgtc tgatggttgt gtgtgtgtgt gtgtgtgtgt
      361 gtttctcaga tgttccacag tgaggactat gagctgttgg ttcttcagca cagctgctgc
      421 ccgttctgcc gcagacccat cgatgagtcc tgctaacaca cactcatgtt taccgcggtg
      481 tgatcatcag tcctgctaac acacactcat gtttaccgcg gtgtgatcca ccctgagccg
      541 ctgtaaagcc gtcagtaaat gccagctcag cacagctctg ataggattac acagatcaac
      601 acaaagcaga ttgcagcgat gctggacaca cacacacaca cacacacaca cactcacagc
      661 tcaacacact cctgcgcatc cacacatccc tcatgttggt gtttttaata aagatttaat
      721 atttcagtct gataatctgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
      781 gtgtgtgtgt gtgtgtgtgt gtgagaccgt gaagtgtccc ttgtgtttgg tcttcatctt
      841 atgaggctca cacaggactt gctgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
      901 tgtgtgtgtg tgttgtccag caggagggtt gtgttctgca ggacacctgt ctgtctgtgg
      961 atgagagatt tcagctctgt gcaggagtgt ttggatggag acgcagcctg cacgcgctga
     1021 tgagcagcag tagtgtgtgt gtgtgtgtcg tgagtgtgtg tggtgagagc gagcgctgct
     1081 gtgtctccat ctctggatta tccccctgtc tgaccccaat ctgaccactc actcctgtaa
     1141 tctgcctaat ctggactcat ggggttcaca taagagcgca gcgccggctc tccgggcttc
     1201 atctgcggag gtgtgccgat cgtccacttc tccatcatgg tgagcaaggg cgaggagctg
     1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
     1441 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatcagc
     1681 gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1921 gggatcactc tcggcatgga cgagctgtag gtaccaaggg cgaattcgac ccaagtttgt
     1981 acaaaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     2041 tgcataaaaa acagactaca taatactgta aaacacaaca tatgcagtca ctatgaacca
     2101 actacttaga tggtattagt gacctgtaga attaattcga gctctagagc tgcagggcgg
     2161 ccgcgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2221 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     2281 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     2341 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     2401 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     2461 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     2521 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     2581 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     2641 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     2701 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     2761 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     2821 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     2881 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     2941 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3001 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     3061 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     3121 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     3181 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3421 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3481 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3541 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3601 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3661 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3721 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3781 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3841 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3901 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3961 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4021 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     4081 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     4141 tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa
     4201 aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt
     4261 cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt
     4321 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc
     4381 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc
     4441 atggatgttt tcccagtcac gacgtt
//
