LOCUS       Exported                4464 bp ds-DNA     circular SYN 23-JUN-2008
DEFINITION  pENTR5 TOPO Sequence sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     See Addgene Vectorpedia pENTR5 TOPO Sequence sequence at 
            www.addgene.org
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13_forward20_primer
     misc_recomb     complement(56..139)
                     /label=attL4
                     /note="/vntifkey=21"
     primer_bind     170..197
                     /label=mcherry-STOP-Asp7181 rv
     CDS             complement(178..885)
                     /codon_start=1
                     /label=mcherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     promoter        complement(887..1941)
                     /label=exorh
     primer_bind     complement(1922..1953)
                     /label=ASP7181-exorh+GFP fw
     misc_feature    1955
                     /label=TOPO Insert
                     /label=nonstandard type:  insertion_seq
                     /note="Insertion site between two As."
                     /note="/vntifkey=14"
     misc_recomb     complement(1993..2076)
                     /label=attR1
                     /note="/vntifkey=21"
     promoter        complement(2170..2188)
                     /label=T7_promoter
                     /note="/vntifkey=30"
     primer_bind     complement(2189..2207)
                     /label=M13_reverse_primer
     CDS             2322..3131
                     /codon_start=1
                     /gene="KanR2"
                     /label=KanR2
                     /note="Orf frame 3"
                     /note="/vntifkey=4"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3262..3881
                     /label=pBR322_origin
                     /note="/vntifkey=33"
     terminator      complement(4196..4223)
                     /label=rrnB_T2_terminator
                     /note="/vntifkey=43"
     terminator      complement(4355..4398)
                     /label=rrnB_T1_terminator
                     /note="/vntifkey=43"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc ccgagttaac gctaccatgg agctccaaat
       61 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
      121 ttttataatg ccaactttgt atagaaaagt tggctccgaa ttcgcccttg gtacctactt
      181 gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgctcgt actgttccac
      241 gatggtgtag tcctcgttgt gggaggtgat gtccagcttg atgttgacgt tgtaggcgcc
      301 gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcggcgt cgtagtggcc
      361 gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta
      421 catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga
      481 ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga
      541 gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa
      601 gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc
      661 cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccgcc
      721 cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc
      781 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
      841 ctccttgatg atggccatgt tgtcctcctc gcccttgctc accatggatg gagaagtgga
      901 cgatcggcac acctccgcag atgaagcccg gagagccggc gctgcgctct tatgtgaacc
      961 ccatgagtcc agattaggca gattacagga gtgagtggtc agattggggt cagacagggg
     1021 gataatccag agatggagac acagcagcgc tcgctctcac cacacacact cacgacacac
     1081 acacacacac tactgctgct catcagcgcg tgcaggctgc gtctccatcc aaacactcct
     1141 gcacagagct gaaatctctc atccacagac agacaggtgt cctgcagaac acaaccctcc
     1201 tgctggacaa cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca
     1261 gcaagtcctg tgtgagcctc ataagatgaa gaccaaacac aagggacact tcacggtctc
     1321 acacacacac acacacacac acacacacac acacacacac acacacacac acacacacac
     1381 acacagatta tcagactgaa atattaaatc tttattaaaa acaccaacat gagggatgtg
     1441 tggatgcgca ggagtgtgtt gagctgtgag tgtgtgtgtg tgtgtgtgtg tgtgtgtcca
     1501 gcatcgctgc aatctgcttt gtgttgatct gtgtaatcct atcagagctg tgctgagctg
     1561 gcatttactg acggctttac agcggctcag ggtggatcac accgcggtaa acatgagtgt
     1621 gtgttagcag gactgatgat cacaccgcgg taaacatgag tgtgtgttag caggactcat
     1681 cgatgggtct gcggcagaac gggcagcagc tgtgctgaag aaccaacagc tcatagtcct
     1741 cactgtggaa catctgagaa acacacacac acacacacac acacaaccat cagacacaca
     1801 acaaagcata tagagtcaaa cagacaaata acagaggaga cacacacaca cacacacaca
     1861 gatgagcgca cctggaagca ggatgggcac atggtgatgc tgacgtcggg caggagtgag
     1921 cggtagtact gccagctgag cggtaccaga tctaagggcg aattcgaccc aagtttgtac
     1981 aaaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     2041 cataaaaaac agactacata atactgtaaa acacaacata tgcagtcact atgaaccaac
     2101 tacttagatg gtattagtga cctgtagaat taattcgagc tctagagctg cagggcggcc
     2161 gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     2221 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     2281 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     2341 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2401 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     2461 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2521 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2581 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     2641 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     2701 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     2761 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     2821 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     2881 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     2941 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     3001 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     3061 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     3121 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     3181 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3421 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3661 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3781 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3841 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3901 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3961 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4021 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4081 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4141 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4201 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4261 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4321 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4381 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     4441 ggatgttttc ccagtcacga cgtt
//
