LOCUS       Exported                3455 bp ds-DNA     circular SYN 06-JAN-2004
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..16
                     /label=M13 (-20) forward primer
     misc_recomb     33..132
                     /label=attL1
                     /note="/invitrogen=2670000"
                     /note="/vntifkey=86"
     protein_bind    141
                     /label=TOPO(R) binding site
                     /note="/invitrogen=2470000"
                     /note="/vntifkey=31"
     promoter        155..289
                     /label=mouse beta-globin minimal promoter
     protein_bind    join(291..1027,142..290)
                     /label=directional TOPO(R) overhang
                     /note="/invitrogen=2410000"
                     /note="/vntifkey=31"
     CDS             302..1018
                     /codon_start=1
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(1028..1032)
                     /label=TOPO(R) binding site
                     /note="/invitrogen=2460000"
                     /note="/vntifkey=31"
     misc_recomb     complement(1044..1143)
                     /label=attL2
                     /note="/invitrogen=2680000"
                     /note="/vntifkey=86"
     promoter        complement(1163..1179)
                     /label=T7 promoter
                     /note="/invitrogen=2370000"
                     /note="/vntifkey=30"
     primer_bind     complement(1184..1200)
                     /label=M13 reverse primer
     CDS             1313..2122
                     /codon_start=1
                     /label=Kan(R)
                     /note="/invitrogen=1210000"
                     /note="/vntifkey=4"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2243..2916
                     /label=pUC origin
                     /note="/invitrogen=2540000"
                     /note="/vntifkey=33"
     terminator      3187..3214
                     /label=rrnB T2 transcription terminator
                     /note="/invitrogen=2590000"
                     /note="/vntifkey=43"
     terminator      3346..3389
                     /label=rrnB T1 transcription terminator
                     /note="/invitrogen=2580000"
                     /note="/vntifkey=43"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccgcggcc cacccttgga tctccccttc caatctgctc agagaggaca
      181 gagtgggcag gagccagcat tgggtatata aagctgagca gggtcagttg cttcttacgt
      241 ttgcttctga gtctgttgtg ttgacttgca acctcagaaa cagacatccg gatccgccac
      301 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      361 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      421 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      481 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      541 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      601 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      661 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      721 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      781 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      841 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      901 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      961 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagct
     1021 cgagtttaag ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataagaaa
     1081 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     1141 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     1201 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     1261 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     1321 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     1381 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     1441 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     1501 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     1561 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     1621 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     1681 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     1741 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     1801 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     1861 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     1921 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     1981 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     2041 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     2101 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     2161 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
     2221 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2281 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2341 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2401 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2461 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2521 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2581 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2641 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2701 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2761 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2821 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2881 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2941 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3001 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     3061 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     3121 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
     3181 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
     3241 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
     3301 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     3361 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
     3421 aacgctagca tggatgtttt cccagtcacg acgtt
//
