LOCUS       Exported                3446 bp ds-DNA     circular SYN 06-JAN-2004
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..16
                     /label=M13 (-20) forward primer
     misc_recomb     33..132
                     /label=attL1
                     /note="/invitrogen=2670000"
                     /note="/vntifkey=86"
     promoter        155..289
                     /label=mouse beta-globin minimal promoter
     protein_bind    join(290..1021,155..289)
                     /label=directional TOPO(R) overhang
                     /note="/invitrogen=2410000"
                     /note="/vntifkey=31"
     CDS             302..1012
                     /codon_start=1
                     /label=Cerulean
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDEL"
     protein_bind    complement(1022..1026)
                     /label=TOPO(R) binding site
                     /note="/invitrogen=2460000"
                     /note="/vntifkey=31"
     misc_recomb     complement(1038..1137)
                     /label=attL2
                     /note="/invitrogen=2680000"
                     /note="/vntifkey=86"
     promoter        complement(1157..1173)
                     /label=T7 promoter
                     /note="/invitrogen=2370000"
                     /note="/vntifkey=30"
     primer_bind     complement(1178..1194)
                     /label=M13 reverse primer
     CDS             1307..2113
                     /codon_start=1
                     /label=Kan(R)
                     /note="/invitrogen=1210000"
                     /note="/vntifkey=4"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2234..2907
                     /label=pUC origin
                     /note="/invitrogen=2540000"
                     /note="/vntifkey=33"
     terminator      3178..3205
                     /label=rrnB T2 transcription terminator
                     /note="/invitrogen=2590000"
                     /note="/vntifkey=43"
     terminator      3337..3380
                     /label=rrnB T1 transcription terminator
                     /note="/invitrogen=2580000"
                     /note="/vntifkey=43"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccgcggcc gcccccttag atctcccttc caatctgctc agagaggaca
      181 gagtgggcag gagccagcat tgggtatata aagctgagca gggtcagttg cttcttacgt
      241 ttgcttctga gtctgttgtg ttgacttgca acctcagaaa cagacatccg gatccgccac
      301 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      361 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      421 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      481 cctcgtgacc accctgacct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
      541 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      601 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      661 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      721 caagctggag tacaacgcca tcagcgacaa cgtctatatc accgccgaca agcagaagaa
      781 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      841 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      901 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
      961 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tggccctcga
     1021 gaagggtggg cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
     1081 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     1141 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     1201 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     1261 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     1321 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     1381 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     1441 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     1501 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     1561 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     1621 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     1681 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     1741 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     1801 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     1861 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     1921 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     1981 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     2041 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     2101 cgatgagttt ttctcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     2161 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     2221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2341 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2401 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2461 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2521 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2581 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2641 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2701 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2761 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2821 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2881 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2941 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3001 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     3061 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3121 tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa
     3181 aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt
     3241 cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt
     3301 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc
     3361 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc
     3421 atggatgttt tcccagtcac gacgtt
//
