LOCUS       Exported                3314 bp ds-DNA     circular SYN 06-JAN-2004
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..16
                     /label=M13 (-20) forward primer
     misc_recomb     33..132
                     /label=attL1
                     /note="/invitrogen=2670000"
                     /note="/vntifkey=86"
     promoter        144..278
                     /label=mouse beta-globin minimal promoter
     protein_bind    join(282..889,144..278)
                     /label=directional TOPO(R) overhang
                     /note="/invitrogen=2410000"
                     /note="/vntifkey=31"
     CDS             302..889
                     /codon_start=1
                     /label=kalta4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSSRSTPSPADALDDGDL
                     DMLPADALDDFDLDMLPADALDDYDLDILPGN"
     protein_bind    complement(890..894)
                     /label=TOPO(R) binding site
                     /note="/invitrogen=2460000"
                     /note="/vntifkey=31"
     misc_recomb     complement(906..1005)
                     /label=attL2
                     /note="/invitrogen=2680000"
                     /note="/vntifkey=86"
     promoter        complement(1025..1041)
                     /label=T7 promoter
                     /note="/invitrogen=2370000"
                     /note="/vntifkey=30"
     primer_bind     complement(1046..1062)
                     /label=M13 reverse primer
     CDS             1175..1981
                     /codon_start=1
                     /label=Kan(R)
                     /note="/invitrogen=1210000"
                     /note="/vntifkey=4"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2102..2775
                     /label=pUC origin
                     /note="/invitrogen=2540000"
                     /note="/vntifkey=33"
     terminator      3046..3073
                     /label=rrnB T2 transcription terminator
                     /note="/invitrogen=2590000"
                     /note="/vntifkey=43"
     terminator      3205..3248
                     /label=rrnB T1 transcription terminator
                     /note="/invitrogen=2580000"
                     /note="/vntifkey=43"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccgcggcc gcccatggcc aatctgctca gagaggacag agtgggcagg
      181 agccagcatt gggtatataa agctgagcag ggtcagttgc ttcttacgtt tgcttctgag
      241 tctgttgtgt tgacttgcaa cctcagaaac agacatccca tgggcggccg cccccttcac
      301 catgaaactg ctctcatcca tcgagcaagc ctgcgacatt tgtcggctta agaagctgaa
      361 atgctccaag gaaaagccga aatgtgccaa atgcctgaag aacaattggg aatgtcgtta
      421 ctctcccaaa accaagcgaa gtccactcac aagggctcat ctgaccgaag tggagagcag
      481 gctagagaga ctggaacaac tctttttgct catcttccct agagaggacc ttgacatgat
      541 cctcaagatg gattctctcc aggatattaa agcccttttg actggcttat tcgtccagga
      601 caatgtgaac aaagacgctg tgacagaccg attggcaagt gtcgagaccg atatgcctct
      661 gacactgaga cagcacagaa tcagcgctac ttcctcaagc gaagagtctt ctaacaaggg
      721 acagagacag ctgactgttt cgagcaggtc gaccccgtcc ccggccgacg ccctggacga
      781 cggcgacctg gacatgctgc ctgctgatgc tctcgatgat ttcgatctgg atatgctccc
      841 ggccgacgcc ctggacgact acgacctgga catcctcccg ggtaactaaa agggtgggcg
      901 cgccgaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
      961 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     1021 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     1081 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     1141 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     1201 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1261 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     1321 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1381 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1441 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     1501 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1561 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     1621 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     1681 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     1741 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     1801 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     1861 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     1921 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     1981 ctcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     2041 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     2101 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2161 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2221 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2281 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2341 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2401 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2461 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     2521 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2581 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2641 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2701 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2761 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2821 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2881 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     2941 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     3001 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
     3061 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
     3121 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
     3181 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
     3241 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
     3301 ccagtcacga cgtt
//
