LOCUS       Exported                3179 bp ds-DNA     circular SYN 06-JAN-2004
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..16
                     /label=M13 (-20) forward primer
     misc_recomb     33..132
                     /label=attL1
                     /note="/invitrogen=2670000"
                     /note="/vntifkey=86"
     protein_bind    join(147..754,144..145)
                     /label=directional TOPO(R) overhang
                     /note="/invitrogen=2410000"
                     /note="/vntifkey=31"
     CDS             167..754
                     /codon_start=1
                     /label=kalta4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSSRSTPSPADALDDGDL
                     DMLPADALDDFDLDMLPADALDDYDLDILPGN"
     protein_bind    complement(755..759)
                     /label=TOPO(R) binding site
                     /note="/invitrogen=2460000"
                     /note="/vntifkey=31"
     misc_recomb     complement(771..870)
                     /label=attL2
                     /note="/invitrogen=2680000"
                     /note="/vntifkey=86"
     promoter        complement(890..906)
                     /label=T7 promoter
                     /note="/invitrogen=2370000"
                     /note="/vntifkey=30"
     primer_bind     complement(911..927)
                     /label=M13 reverse primer
     CDS             1040..1846
                     /codon_start=1
                     /label=Kan(R)
                     /note="/invitrogen=1210000"
                     /note="/vntifkey=4"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1967..2640
                     /label=pUC origin
                     /note="/invitrogen=2540000"
                     /note="/vntifkey=33"
     terminator      2911..2938
                     /label=rrnB T2 transcription terminator
                     /note="/invitrogen=2590000"
                     /note="/vntifkey=43"
     terminator      3070..3113
                     /label=rrnB T1 transcription terminator
                     /note="/invitrogen=2580000"
                     /note="/vntifkey=43"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccgcggcc gcccatgggc ggccgccccc ttcaccatga aactgctctc
      181 atccatcgag caagcctgcg acatttgtcg gcttaagaag ctgaaatgct ccaaggaaaa
      241 gccgaaatgt gccaaatgcc tgaagaacaa ttgggaatgt cgttactctc ccaaaaccaa
      301 gcgaagtcca ctcacaaggg ctcatctgac cgaagtggag agcaggctag agagactgga
      361 acaactcttt ttgctcatct tccctagaga ggaccttgac atgatcctca agatggattc
      421 tctccaggat attaaagccc ttttgactgg cttattcgtc caggacaatg tgaacaaaga
      481 cgctgtgaca gaccgattgg caagtgtcga gaccgatatg cctctgacac tgagacagca
      541 cagaatcagc gctacttcct caagcgaaga gtcttctaac aagggacaga gacagctgac
      601 tgtttcgagc aggtcgaccc cgtccccggc cgacgccctg gacgacggcg acctggacat
      661 gctgcctgct gatgctctcg atgatttcga tctggatatg ctcccggccg acgccctgga
      721 cgactacgac ctggacatcc tcccgggtaa ctaaaagggt gggcgcgccg acccagcttt
      781 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
      841 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
      901 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
      961 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     1021 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     1081 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     1141 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     1201 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     1261 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     1321 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     1381 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     1441 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     1501 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     1561 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     1621 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     1681 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     1741 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     1801 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctcag aattggttaa
     1861 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     1921 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     1981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2101 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2341 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     2401 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2461 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2521 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2581 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2641 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2701 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2761 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2821 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
     2881 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     2941 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     3001 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     3061 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
     3121 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgtt
//
