LOCUS       Exported                4885 bp ds-DNA     circular SYN 12-AUG-2009
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..16
                     /label=M13 (-20) forward primer
     misc_recomb     33..132
                     /label=attL1
                     /note="/invitrogen=2670000"
                     /note="/vntifkey=86"
     promoter        144..278
                     /label=mouse beta globin minimal promoter
     protein_bind    290..294
                     /label=TOPO binding site
                     /note="/invitrogen=2470000"
                     /note="/vntifkey=31"
     protein_bind    295..298
                     /label=directional TOPO overhang
                     /note="/invitrogen=2410000"
                     /note="/vntifkey=31"
     CDS             299..2458
                     /codon_start=1
                     /label=Cre-ERT2
                     /note="/ApEinfo_fwdcolor=#ff3235"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=4"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGDLEPSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPT
                     RPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVW
                     RSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSII
                     LLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLIL
                     SHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLAT
                     AGSTSSHSLQKYYITGEAEGFPATA**RSELPGGIRILLKQNLFIAAYNGYK*SNSITN
                     FTNKAFFSLHSSCGLSKLINVSYHV"
     protein_bind    complement(2459..2462)
                     /label=TOPO binding site
                     /note="/invitrogen=2460000"
                     /note="/vntifkey=31"
     misc_recomb     complement(2474..2573)
                     /label=attL2
                     /note="/invitrogen=2680000"
                     /note="/vntifkey=86"
     promoter        complement(2593..2609)
                     /label=T7 promoter
                     /note="/invitrogen=2370000"
                     /note="/vntifkey=30"
     primer_bind     complement(2614..2630)
                     /label=M13 reverse primer
     CDS             2743..3552
                     /codon_start=1
                     /label=Kan(R)
                     /note="/invitrogen=1210000"
                     /note="/vntifkey=4"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3673..4346
                     /label=pUC origin
                     /note="/invitrogen=2540000"
                     /note="/vntifkey=33"
     terminator      4617..4644
                     /label=rrnB T2 transcription terminator
                     /note="/invitrogen=2590000"
                     /note="/vntifkey=43"
     terminator      4776..4819
                     /label=rrnB T1 transcription terminator
                     /note="/invitrogen=2580000"
                     /note="/vntifkey=43"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccgcggcc gcccccttcc aatctgctca gagaggacag agtgggcagg
      181 agccagcatt gtgtatataa agctgagcag ggtcagttgc ttcttacgtt tgcttctgag
      241 tctgttgtgt tgacttgcaa cctcagaaac agacatccca tgggccgccc ccttcaccat
      301 gtccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg caacgagtga
      361 tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt ctgagcatac
      421 ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt tgaataaccg
      481 gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg
      541 cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg
      601 gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc ggcggatccg
      661 aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga
      721 tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc tgccaggata tacgtaatct
      781 ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg ccaggatcag
      841 ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg gcagaacgaa
      901 aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt
      961 cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc tgttttgccg
     1021 ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct
     1081 ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg actctggtca
     1141 gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg
     1201 cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg taaatattgt
     1261 catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc tgctggaaga
     1321 tggcgatctc gagccatctg ctggagacat gagagctgcc aacctttggc caagcccgct
     1381 catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg accagatggt
     1441 cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc ctaccagacc
     1501 cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg agctggttca
     1561 catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc atgatcaggt
     1621 ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct ggcgctccat
     1681 ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga accagggaaa
     1741 atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat ctcggttccg
     1801 catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt tgcttaattc
     1861 tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg accatatcca
     1921 ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg caggcctgac
     1981 cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc acatcaggca
     2041 catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg tggtgcccct
     2101 ctatgacctg ctgctggagg cggcggacgc ccaccgccta catgcgccca ctagccgtgg
     2161 aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct ctacttcatc
     2221 gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg ccacagcttg
     2281 atgaagatct gagctccctg gcggaattcg gatcttatta aagcagaact tgtttattgc
     2341 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     2401 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgaag
     2461 ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     2521 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     2581 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     2641 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     2701 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     2761 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     2821 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     2881 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     2941 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     3001 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     3061 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     3121 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     3181 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     3241 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     3301 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     3361 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     3421 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     3481 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     3541 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     3601 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     3661 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3721 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3781 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3841 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3901 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3961 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4021 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4081 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4141 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4201 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4261 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4321 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4381 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4441 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4501 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     4561 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     4621 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     4681 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     4741 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     4801 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     4861 tggatgtttt cccagtcacg acgtt
//
