LOCUS       Exported                3721 bp ds-DNA     circular UNA 12-MAR-2008
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     1..17
                     /label=M13F
     misc_recomb     55..211
                     /label=attR2
     region          214..279
                     /label=PTV1-2A peptide
                     /note="/region_type=Peptide"
     CDS             280..996
                     /label=EGFP
     misc_feature    997..1056
                     /label=pCS2 MCS fragment
     polyA_signal    1097..1292
                     /label=SV40 late polyA (from pCS2+)
     misc_recomb     1294..1389
                     /label=attL3
     promoter        complement(1423..1442)
                     /label=T7
     primer_bind     complement(1446..1464)
                     /label=M13R
     CDS             1579..2388
                     /label=kanR
     rep_origin      2509..3182
                     /label=pUC ori
     terminator      3453..3480
                     /label=T2
     terminator      3612..3655
                     /label=T1
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cctgcagctc tagagctcga attctacagg
       61 tcactaatac catctaagta gttgattcat agtgactgca tatgttgtgt tttacagtat
      121 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
      181 gtttctcgtt caactttctt gtacaaagtg gggggatccg gagccacgaa cttctctctg
      241 ttaaagcaag caggagacgt ggaagaaaac cccggtccta tggtgagcaa gggcgaggag
      301 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      361 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      421 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      481 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      541 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      601 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      661 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      721 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      781 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      841 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
      901 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      961 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtggaagatc tgggaattca
     1021 aggcctctcg agcctctaga ttctgcagcc ctatagtgag gcgcgcctct agaactatag
     1081 tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     1141 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     1201 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     1261 tcaggttcag ggggaggtgt gggaggtttt ttccaacttt attatacaaa gttggcatta
     1321 taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1381 tcattatttg gagctccatg gtagcgttaa cgcggccgcg atatccccta tagtgagtcg
     1441 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     1501 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     1561 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     1621 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1681 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     1741 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     1801 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     1861 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     1921 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     1981 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2041 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2101 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2161 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2221 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2281 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2341 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     2401 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     2461 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     2521 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2581 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2641 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2701 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2761 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2821 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2881 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     2941 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3001 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3061 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3121 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3181 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3241 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3301 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3361 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     3421 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     3481 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     3541 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     3601 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     3661 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     3721 t
//
