LOCUS       Exported                3009 bp ds-DNA     circular SYN 03-JUL-2013
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..16
                     /label=M13F
     misc_recomb     28..184
                     /label=attR2
     region          187..252
                     /label=PTV-1 2A peptide
                     /note="/region_type=Peptide"
     misc_structure  255..310
                     /label=MCS from pCS2FA
     polyA_signal    334..531
                     /label=SV40 late polyA (from pCS2+)
     misc_recomb     587..682
                     /label=attL3
     promoter        complement(711..730)
                     /label=T7
     primer_bind     complement(734..754)
                     /label=M13R
     CDS             867..1676
                     /codon_start=1
                     /label=kanR
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1797..2470
                     /label=pUC ori
     terminator      2741..2768
                     /label=T2
     terminator      2900..2943
                     /label=T1
ORIGIN
        1 gtaaaacgac ggccagtctt aaaattctac aggtcactaa taccatctaa gtagttggtt
       61 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
      121 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      181 gtggggggat ctggagccac gaacttctct ctgttaaagc aagcaggaga cgtggaagaa
      241 aaccccggtc ctagggatcc catcgattcg aattcaaggc ctctcgagcc tctagtggcg
      301 cgcctctaga actatagtga gtcgtattac gtagatccag acatgataag atacattgat
      361 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      421 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      481 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc
      541 gcggcgccaa tgcattgggc ccggtaccga tccaccggat cttgaccaac tttattatac
      601 aaagttggca ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
      661 agtcaaaata aaatcattat ttggagctcc atggtagcgt taacgcggcc atcccctata
      721 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
      781 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
      841 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
      901 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
      961 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
     1021 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     1081 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     1141 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
     1201 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     1261 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     1321 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     1381 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     1441 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     1501 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     1561 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     1621 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     1681 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa
     1741 gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     1801 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1861 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1921 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1981 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2041 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2101 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2161 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     2221 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2281 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2341 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2401 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2461 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2521 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2581 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2641 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2701 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     2761 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     2821 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     2881 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     2941 tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt
     3001 cacgacgtt
//
