LOCUS       Exported                6785 bp ds-DNA     circular UNA 10-JUN-2008
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     394.pDestTol2pA2, Tol2kit v1.0 ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     1..22
                     /label=M13R
     primer_bind     1..22
                     /label=M13R(1)
     misc_recomb     37..57
                     /label=attB4
     promoter        75..1129
                     /label=exorh
     misc_recomb     1146..1166
                     /label=attB1
     CDS             1175..1891
                     /label=EGFP
                     /note="/vntifkey=4"
     CDS             1892..1951
                     /label=trailer
     misc_recomb     complement(1956..1976)
                     /label=attB2
     3'UTR           2023..2202
                     /label=ubi 3' UTR
     CDS             2023..2025
                     /label=ubi STOP codon
                     /note="/cds_type=ORF"
     misc_feature    2203..2520
                     /label=ubi 3' locus
                     /note="/feature_type=Genomic Region"
     misc_recomb     complement(2622..2642)
                     /label=attB3
     primer_bind     complement(2651..2666)
                     /label=M13F
     polyA_signal    2682..2879
                     /label=SV40 late polyA (from pCS2+)
                     /note="/vntifkey=25"
     misc_structure  complement(2938..3272)
                     /label=Tol2 exon1 bp1-335 of 461
                     /note="/vntifkey=88"
     LTR             complement(3436..3454)
                     /label=Tol2 5' TIR
                     /note="/vntifkey=19"
     promoter        complement(3540..3559)
                     /label=T3
                     /note="/vntifkey=30"
     primer_bind     complement(3575..3596)
                     /label=M13R
     primer_bind     complement(3575..3596)
                     /label=M13R(1)
     rep_origin      complement(3923..4596)
                     /direction=LEFT
                     /label=pUC ori
                     /note="/vntifkey=33"
     rep_origin      complement(3964..4646)
                     /direction=LEFT
                     /label=colE1 ori
                     /note="/vntifkey=33"
     CDS             complement(4744..5601)
                     /label=ampR
                     /note="/vntifkey=4"
     LTR             complement(6239..6255)
                     /label=Tol2 3' TIR
                     /note="/vntifkey=19"
     misc_structure  complement(6428..6774)
                     /label=Tol2 exon4 136-482
                     /note="/vntifkey=88"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttggct
       61 ccgaattcgc ccttgctcag ctggcagtac taccgctcac tcctgcccga cgtcagcatc
      121 accatgtgcc catcctgctt ccaggtgcgc tcatctgtgt gtgtgtgtgt gtgtgtctcc
      181 tctgttattt gtctgtttga ctctatatgc tttgttgtgt gtctgatggt tgtgtgtgtg
      241 tgtgtgtgtg tgtgtttctc agatgttcca cagtgaggac tatgagctgt tggttcttca
      301 gcacagctgc tgcccgttct gccgcagacc catcgatgag tcctgctaac acacactcat
      361 gtttaccgcg gtgtgatcat cagtcctgct aacacacact catgtttacc gcggtgtgat
      421 ccaccctgag ccgctgtaaa gccgtcagta aatgccagct cagcacagct ctgataggat
      481 tacacagatc aacacaaagc agattgcagc gatgctggac acacacacac acacacacac
      541 acacactcac agctcaacac actcctgcgc atccacacat ccctcatgtt ggtgttttta
      601 ataaagattt aatatttcag tctgataatc tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
      661 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgagac cgtgaagtgt cccttgtgtt
      721 tggtcttcat cttatgaggc tcacacagga cttgctgtgt gtgtgtgtgt gtgtgtgtgt
      781 gtgtgtgtgt gtgtgtgtgt gtgtgttgtc cagcaggagg gttgtgttct gcaggacacc
      841 tgtctgtctg tggatgagag atttcagctc tgtgcaggag tgtttggatg gagacgcagc
      901 ctgcacgcgc tgatgagcag cagtagtgtg tgtgtgtgtg tcgtgagtgt gtgtggtgag
      961 agcgagcgct gctgtgtctc catctctgga ttatccccct gtctgacccc aatctgacca
     1021 ctcactcctg taatctgcct aatctggact catggggttc acataagagc gcagcgccgg
     1081 ctctccgggc ttcatctgcg gaggtgtgcc gatcgtccac ttctccatca agggcgaatt
     1141 cgacccaagt ttgtacaaaa aagcaggctg gaccatggtg agcaagggcg aggagctgtt
     1201 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1261 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1321 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     1381 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1441 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1501 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1561 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1621 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1681 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1741 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1801 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1861 gatcactctc ggcatggacg agctgtacaa gggcggtgga agatctggga attcaaggcc
     1921 tctcgagcct ctagattctg cagccctata gcacccagct ttcttgtaca aagtgggcgg
     1981 ccgctctaga actagtggat cttctagaaa gattgagtaa ggtagaaccg acagtcttag
     2041 ggatggacat taaattctca gtatcccctg cttatgcact caccactttt tcccattatt
     2101 ttattcatat tgtgttgcta aatccaacta cattcgtatt accatgttct tttattagtt
     2161 tttttttatc tcagctgtca aaataaaagc attgaaatac aacattgttc tatttttcat
     2221 tgtggttttg catgcccggt aaataaaaaa acaaggtaat gaaaataaga tgcgaaatga
     2281 gaagtatctc attatgctgt aagaagcata attagaatat gcaagtagct ttccaaattt
     2341 taggtgtctt gtctttctgg gcttttaaag tgtttattca tgtatattta tagatgggaa
     2401 tttttctgct tactgcttta aaatgttata gcagctgctg aactttggtg gttctcttta
     2461 ttgtgttgta tgaacacaga aaatggctaa aaatcgctaa cacttgatgg caatgaattc
     2521 atcttgctga aaaactcgag ccatccggaa gatcccccgg gctgcaggaa ttcgatatca
     2581 agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaactttat tatacatagt
     2641 tgataattca ctggccgtcg ttttacggta ccatcgatga tgatccagac atgataagat
     2701 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2761 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2821 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     2881 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatctgcg
     2941 aagatacggc cacgggtgct cttgatcctg tggctgattt tggactgtgc tgctcgcagc
     3001 tgctgatgaa tcacatactt cctccatttt cttccactga ttgactgtta taatttccct
     3061 aatttccagg tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg tcacttccaa
     3121 aggaccaatg aacatgtctg accaatttca tataatgtga aaacgatttt cataggcaga
     3181 ataaataaca tttaaattaa actgggcatc agcgcaattc aattggtttg gtaatagcaa
     3241 gggaaaatag aatgaagtga tctccaaaaa ataagtactt tttgactgta aataaaattg
     3301 taaggagtaa aaagtacttt tttttctaaa aaaatgtaat taagtaaaag taaaagtatt
     3361 gatttttaat tgtactcaag taaagtaaaa atccccaaaa ataatactta agtacagtaa
     3421 tcaagtaaaa ttactcaagt actttacacc tctggttctt gaccccctac cttcagcaag
     3481 cccagcagat ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc
     3541 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3721 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3961 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4381 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4501 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4561 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4621 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4681 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4741 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4801 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4861 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4921 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4981 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5041 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5101 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5161 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5221 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5281 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5341 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5401 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5461 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5521 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5581 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5641 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5701 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     5761 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     5821 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     5881 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     5941 ggcgatggcc cacgctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc
     6001 aatgcaccaa ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg
     6061 aatggagact acctcctgtc caacaaggct cttggatacg agtacgccta cctgttggac
     6121 ccaggtcatt gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt
     6181 cagacaccca tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca
     6241 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
     6301 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
     6361 tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac
     6421 tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt
     6481 ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg
     6541 ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct
     6601 caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga
     6661 aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc
     6721 aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggccc
     6781 tcgag
//
