LOCUS       Exported                9711 bp ds-DNA     circular UNA 10-JUN-2008
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
REFERENCE   2  (bases 1 to 9711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     302.p3E-polyA, Tol2kit v1.0 ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..9711
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     1..22
                     /label=M13R
     primer_bind     1..22
                     /label=M13R(1)
     misc_recomb     37..57
                     /label=attB4
     promoter        93..2595
                     /label=desmin promoter region
     5'UTR           2552..2595
                     /label=desmin 5' UTR
     misc_recomb     2618..2638
                     /label=attB1
     promoter        2664..2798
                     /label=mouse beta-globin minimal promoter
     CDS             2811..3518
                     /label=mApple
                     /note="/cds_type=ORF"
     misc_recomb     complement(3547..3567)
                     /label=attB2
     polyA_signal    3570..3765
                     /label=SV40 late polyA (from pCS2+)
                     /note="/vntifkey=25"
     misc_recomb     complement(3767..3787)
                     /label=attB3
     primer_bind     complement(3796..3811)
                     /label=M13F
     promoter        3818..4872
                     /label=exorh promoter
     CDS             4873..5592
                     /label=EGFP
     polyA_signal    5608..5805
                     /label=SV40 late polyA (from pCS2+)
                     /note="/vntifkey=25"
     misc_structure  complement(5864..6198)
                     /label=Tol2 exon1 bp1-335 of 461
                     /note="/vntifkey=88"
     LTR             complement(6362..6380)
                     /label=Tol2 5' TIR
                     /note="/vntifkey=19"
     promoter        complement(6466..6485)
                     /label=T3
                     /note="/vntifkey=30"
     primer_bind     complement(6501..6522)
                     /label=M13R
     primer_bind     complement(6501..6522)
                     /label=M13R(1)
     rep_origin      complement(6849..7522)
                     /direction=LEFT
                     /label=pUC ori
                     /note="/vntifkey=33"
     rep_origin      complement(6890..7572)
                     /direction=LEFT
                     /label=colE1 ori
                     /note="/vntifkey=33"
     CDS             complement(7670..8527)
                     /label=ampR
                     /note="/vntifkey=4"
     LTR             complement(9165..9181)
                     /label=Tol2 3' TIR
                     /note="/vntifkey=19"
     misc_structure  complement(9354..9700)
                     /label=Tol2 exon4 136-482
                     /note="/vntifkey=88"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttggct
       61 ccgaattcgc ccttgatatc actgatgcta gctccttgag gcactttcgg tgtccttaca
      121 aacacagatc ccatacatta cattcacaga tacacaaaag atttaacacc ttatcaattt
      181 atattgtgta taaacacttc ttaataaagt gttttgtgtg tgtgtgtgtg tgtgtgtgtg
      241 tgtgtgtgtg tgtgtgtgtt tctgtctgtc tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
      301 tgtgtgtgtg tgtctgtgtg tgtgatagtc gagtctctgg cctatcaaga ctccattatt
      361 tttccaggag gccttcagga cagtagactg gtgatggggt tcagacggga agaatattaa
      421 tagccatgac agcagcagct gtggagcaac atcacagctt ctcggttact atggtcagcc
      481 tgaggaatgg gccggactga tgggggttga gcgacaggga tcttatttgg gatcgcattt
      541 atccgatgac acacttacat taatcggatt tcccatgtgt aatgtaagag acaaactaat
      601 taagaatatt ccacatgtga aaaaatgatg tggtactact aaaatattag gccttactca
      661 tatggtggtg caatattttg tatgggagga gatgcaagtc acacagcagt gcttgatttc
      721 tgtttcaaca tcctagtaca ttttttttaa tcataattca catattttaa tgcaaccata
      781 tgcacattac tgatgtgata tgtccaaaaa aacaaacaaa ctgcaaatta gtagcacaac
      841 agacacttag agtaagccag gggcagacca ggactaaaaa tcagccctgg cactgtagcc
      901 acatcagccc acattatcag accgacacag ccccaccctc ggacatgcat attcactact
      961 tttattggtg tacagatggt aaaacaatat aagcagtacc atatgtaata gatatttaat
     1021 agatatttat tttgtgtttg gacttgtgag ggaaaccagg aagaaaccca cacgaacatg
     1081 caaactccaa aatgaaacac ctaatgaccc agccgaggct cgaaccattg acctttttgc
     1141 tgtgaggcgg cagcgctacc cattgtgctg cccccttttg agactatttc agttaatttt
     1201 atgcatttgg cagcgtctga ttttagagca atttttatgt ttctctgagc tttttggcac
     1261 cgcctttcct tttttttaca gtccatctct gacaattagc ttgtgttgca cctaatgttt
     1321 atttgacatt cttgccagat tttgattatg tccgcataac ctctgattgg gttggcccat
     1381 ctcgaaacca gctggccatt gccgattggg ccaatgctta acatttatta ttattactat
     1441 tatcatcatc attatcatca tcatcatcat catcatcatc atcatcatca tcatcatcat
     1501 aatattcaca tcttgcaaga aacaaaaact gcctgtatgt aagcaaaaaa taaaaaaaaa
     1561 ttataaaaaa cataacataa aacataaaaa gtatgtaaaa aaaataataa aaaatagcat
     1621 acccgcccac atttaaaaaa tggtccagcc cttttggcat ttgccagaat tgccagatgg
     1681 ccagtccgcc cctggactag gcaaccctga tgccaatatg cttatttcac gcggtcgcca
     1741 ttttaaagaa ccaaagcgaa ggctgcggtg ggaagaaagc tggaattata ggaccagcac
     1801 tgtaaacatt gcagtaaaat gctgtggact acaaacctcc agctgttgcc gagatttgga
     1861 ggtttgtagt ccacagcatg ttactgcaat gtatacagtg ctggtcctat acttatatat
     1921 atatatatac tgaagaaaga tggaaacttg gaacttggta aaacaatgta aactaatgta
     1981 aactttccag cattcttcaa aatatcattt ttgatgttca acagaagaaa aaaaaaggtt
     2041 ttaaacaact tgagggtgac taaattgtga attaattttc atttttgggt gaactatccc
     2101 tttaggatag gaattagata actttttcaa actttgtagt ccttttatat tcataaaaat
     2161 ctaaaaagaa acaatattaa atgaaatatt tatgacagct caatgtggcc acttttaata
     2221 gaaaaccctt ttgctttttc aaacacacac acaaactcat cagtctaata tggtgtttat
     2281 tgggccacaa gcactcttcc tggtaagcag aagtgacagc acagtttggc atggcgtgac
     2341 ctgtgccacc tcatgccttg tccagttgct ccacccagct gctggaaacc agacctcatg
     2401 acagctgagg aagcaaggtt tagaaaagag agagggagag atagaagtgg gcgccaatgt
     2461 gtgtgggttg agaacagtga ctaaaaaaat gtgggtgggg gcaaacggga gtggtctcta
     2521 tatatcccct cttctgctct gcttaaccct cattcttcca cacagtcctc agagacccag
     2581 cattcacaca gcgtagtcga caagggcgaa ttcgacccaa gtttgtacaa aaaagcaggc
     2641 tccgcggccg cccccttaga tctcccttcc aatctgctca gagaggacag agtgggcagg
     2701 agccagcatt gggtatataa agctgagcag ggtcagttgc ttcttacgtt tgcttctgag
     2761 tctgttgtgt tgacttgcaa cctcagaaac agacatccgg atccgccacc atggtgagca
     2821 agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     2881 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     2941 aggcctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     3001 acatcctgtc ccctcagttc atgtacggct ccaaggtcta cattaagcac ccagccgaca
     3061 tccccgacta cttcaagctg tccttccccg agggcttcag gtgggagcgc gtgatgaact
     3121 tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac ggcgtgttca
     3181 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     3241 agaccatggg ctgggaggcc tccgaggagc ggatgtaccc cgaggacggc gccctgaaga
     3301 gcgagatcaa gaagaggctg aagctgaagg acggcggcca ctacgccgcc gaggtcaaga
     3361 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt
     3421 tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     3481 gccgccactc caccggcggc atggacgagc tgtacaaggc cctcgagaag ggtgggcgcg
     3541 ccgacccagc tttcttgtac aaagtggggg atccagacat gataagatac attgatgagt
     3601 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3661 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3721 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttccaac tttattatac
     3781 atagttgata attcactggc cgtcgtttta cggtaccgct cagctggcag tactaccgct
     3841 cactcctgcc cgacgtcagc atcaccatgt gcccatcctg cttccaggtg cgctcatctg
     3901 tgtgtgtgtg tgtgtgtgtc tcctctgtta tttgtctgtt tgactctata tgctttgttg
     3961 tgtgtctgat ggttgtgtgt gtgtgtgtgt gtgtgtgttt ctcagatgtt ccacagtgag
     4021 gactatgagc tgttggttct tcagcacagc tgctgcccgt tctgccgcag acccatcgat
     4081 gagtcctgct aacacacact catgtttacc gcggtgtgat catcagtcct gctaacacac
     4141 actcatgttt accgcggtgt gatccaccct gagccgctgt aaagccgtca gtaaatgcca
     4201 gctcagcaca gctctgatag gattacacag atcaacacaa agcagattgc agcgatgctg
     4261 gacacacaca cacacacaca cacacacact cacagctcaa cacactcctg cgcatccaca
     4321 catccctcat gttggtgttt ttaataaaga tttaatattt cagtctgata atctgtgtgt
     4381 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtga
     4441 gaccgtgaag tgtcccttgt gtttggtctt catcttatga ggctcacaca ggacttgctg
     4501 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtt gtccagcagg
     4561 agggttgtgt tctgcaggac acctgtctgt ctgtggatga gagatttcag ctctgtgcag
     4621 gagtgtttgg atggagacgc agcctgcacg cgctgatgag cagcagtagt gtgtgtgtgt
     4681 gtgtcgtgag tgtgtgtggt gagagcgagc gctgctgtgt ctccatctct ggattatccc
     4741 cctgtctgac cccaatctga ccactcactc ctgtaatctg cctaatctgg actcatgggg
     4801 ttcacataag agcgcagcgc cggctctccg ggcttcatct gcggaggtgt gccgatcgtc
     4861 cacttctcca tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     4921 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     4981 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     5041 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     5101 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     5161 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     5221 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     5281 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     5341 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     5401 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     5461 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     5521 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     5581 ctgtacaagt aaaggtacct cgatgatgat ccagacatga taagatacat tgatgagttt
     5641 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     5701 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     5761 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     5821 tacaaatgtg gtatggctga ttatgatcct ctagatcaga tctgcgaaga tacggccacg
     5881 ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac
     5941 atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa
     6001 ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca
     6061 tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta
     6121 aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg
     6181 aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag
     6241 tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta
     6301 ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac
     6361 tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac
     6421 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     6481 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6541 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6601 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6661 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6721 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6781 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6841 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6901 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6961 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7021 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7081 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7141 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7201 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7261 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7321 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     7381 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7441 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7501 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7561 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7621 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7681 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7741 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7801 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7861 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7921 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7981 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8041 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8101 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8161 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8221 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8281 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8341 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8401 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8461 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8521 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8581 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8641 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     8701 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8761 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8821 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccacg
     8881 ctggctaaga actcatcagc ctccccggtc catctaccca cgtaccaatg caccaattgg
     8941 ccacaatgac ggctactaca tggtgccatt ccttcctctt tataggaatg gagactacct
     9001 cctgtccaac aaggctcttg gatacgagta cgcctacctg ttggacccag gtcattgcac
     9061 aacaccagaa atgccctctg atctgcaaaa gacgtgaata tctgttcaga cacccatatc
     9121 cactctgttc cacacaggtc agaggtttgt ccaggagttc ttgacagagg tgtaaaaagt
     9181 actcaaaaat tttactcaag tgaaagtaca agtacttagg gaaaatttta ctcaattaaa
     9241 agtaaaagta tctggctaga atcttacttg agtaaaagta aaaaagtact ccattaaaat
     9301 tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa gaaaactaga gattcttgtt
     9361 taagctttta atctcaaaaa acattaaatg aaatgcatac aaggttttat cctgctttag
     9421 aactgtttgt atttaattat caaactataa gacagacaat ctaatgccag tacacgctac
     9481 tcaaagttgt aaaacctcag atttaacttc agtagaagct gattctcaaa attgttagtg
     9541 tcaagcctag ctcttttggg gctgaaaagc aatcctgcag tgctgaaaag cctctcacag
     9601 gcagccgatg cgggaagagg tgtattagtc ttgatagaga ggctgcaaat agcaggaaac
     9661 gtgagcagag actccctggt gtctgaaaca caggccagat gggccctcga g
//
