LOCUS       Exported                7655 bp ds-DNA     circular UNA 12-APR-2010
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     394.pDestTol2pA2, Tol2kit v1.0 ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     1..22
                     /label=M13R
     primer_bind     1..22
                     /label=M13R(1)
     misc_recomb     37..161
                     /label=attR4
                     /note="/vntifkey=86"
     misc_feature    complement(201..506)
                     /label=ccdB
                     /label=nonstandard type:  Resistance
                     /note="/vntifkey=22"
     CDS             complement(848..1507)
                     /label=cmR
                     /note="/vntifkey=4"
     misc_recomb     1616..1740
                     /label=attR3
                     /note="/vntifkey=86"
     primer_bind     complement(1749..1764)
                     /label=M13F
     promoter        1771..2825
                     /label=exorh promoter
     CDS             2826..3536
                     /label=cerulean
     polyA_signal    3552..3749
                     /label=SV40 late polyA (from pCS2+)
                     /note="/vntifkey=25"
     misc_structure  complement(3808..4142)
                     /label=Tol2 exon1 bp1-335 of 461
                     /note="/vntifkey=88"
     LTR             complement(4306..4324)
                     /label=Tol2 5' TIR
                     /note="/vntifkey=19"
     promoter        complement(4410..4429)
                     /label=T3
                     /note="/vntifkey=30"
     primer_bind     complement(4445..4466)
                     /label=M13R
     primer_bind     complement(4445..4466)
                     /label=M13R(1)
     rep_origin      complement(4793..5466)
                     /direction=LEFT
                     /label=pUC ori
                     /note="/vntifkey=33"
     rep_origin      complement(4834..5516)
                     /direction=LEFT
                     /label=colE1 ori
                     /note="/vntifkey=33"
     CDS             complement(5614..6471)
                     /label=ampR
                     /note="/vntifkey=4"
     LTR             complement(7109..7125)
                     /label=Tol2 3' TIR
                     /note="/vntifkey=19"
     misc_structure  complement(7298..7644)
                     /label=Tol2 exon4 136-482
                     /note="/vntifkey=88"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgaac
       61 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      121 ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc agactggctg
      181 tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg
      241 tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac cggcacactg
      301 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt
      361 tcacggggga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc
      421 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt
      481 ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcgg caaaggagcc
      541 gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg
      601 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccgaac cggagatatt
      661 gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc
      721 tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta tatattctta
      781 taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc
      841 ctctagatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
      901 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
      961 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1021 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1081 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1141 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1201 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1261 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
     1321 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1381 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1441 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1501 tctccatttt agcttcctta gctcctgaaa atctcgacgg atcctaactc aaaatccaca
     1561 cattatacga gccggaagca taaagtgtaa agcctggggg tgcctaatgc ggccgccata
     1621 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     1681 ttaatatatt gatatttata tcattttacg tttctcgttc aactttatta tacatagttg
     1741 ataattcact ggccgtcgtt ttacggtacc gctcagctgg cagtactacc gctcactcct
     1801 gcccgacgtc agcatcacca tgtgcccatc ctgcttccag gtgcgctcat ctgtgtgtgt
     1861 gtgtgtgtgt gtctcctctg ttatttgtct gtttgactct atatgctttg ttgtgtgtct
     1921 gatggttgtg tgtgtgtgtg tgtgtgtgtg tttctcagat gttccacagt gaggactatg
     1981 agctgttggt tcttcagcac agctgctgcc cgttctgccg cagacccatc gatgagtcct
     2041 gctaacacac actcatgttt accgcggtgt gatcatcagt cctgctaaca cacactcatg
     2101 tttaccgcgg tgtgatccac cctgagccgc tgtaaagccg tcagtaaatg ccagctcagc
     2161 acagctctga taggattaca cagatcaaca caaagcagat tgcagcgatg ctggacacac
     2221 acacacacac acacacacac actcacagct caacacactc ctgcgcatcc acacatccct
     2281 catgttggtg tttttaataa agatttaata tttcagtctg ataatctgtg tgtgtgtgtg
     2341 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgagaccgtg
     2401 aagtgtccct tgtgtttggt cttcatctta tgaggctcac acaggacttg ctgtgtgtgt
     2461 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gttgtccagc aggagggttg
     2521 tgttctgcag gacacctgtc tgtctgtgga tgagagattt cagctctgtg caggagtgtt
     2581 tggatggaga cgcagcctgc acgcgctgat gagcagcagt agtgtgtgtg tgtgtgtcgt
     2641 gagtgtgtgt ggtgagagcg agcgctgctg tgtctccatc tctggattat ccccctgtct
     2701 gaccccaatc tgaccactca ctcctgtaat ctgcctaatc tggactcatg gggttcacat
     2761 aagagcgcag cgccggctct ccgggcttca tctgcggagg tgtgccgatc gtccacttct
     2821 ccatcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2881 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2941 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3001 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca
     3061 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3121 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3181 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3241 ggcacaagct ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga
     3301 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3361 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3421 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     3481 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgaggt
     3541 acctcgatga tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     3601 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     3661 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     3721 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     3781 ctgattatga tcctctagat cagatctgcg aagatacggc cacgggtgct cttgatcctg
     3841 tggctgattt tggactgtgc tgctcgcagc tgctgatgaa tcacatactt cctccatttt
     3901 cttccactga ttgactgtta taatttccct aatttccagg tcaaggtgct gtgcattgtg
     3961 gtaatagatg tgacatgacg tcacttccaa aggaccaatg aacatgtctg accaatttca
     4021 tataatgtga aaacgatttt cataggcaga ataaataaca tttaaattaa actgggcatc
     4081 agcgcaattc aattggtttg gtaatagcaa gggaaaatag aatgaagtga tctccaaaaa
     4141 ataagtactt tttgactgta aataaaattg taaggagtaa aaagtacttt tttttctaaa
     4201 aaaatgtaat taagtaaaag taaaagtatt gatttttaat tgtactcaag taaagtaaaa
     4261 atccccaaaa ataatactta agtacagtaa tcaagtaaaa ttactcaagt actttacacc
     4321 tctggttctt gaccccctac cttcagcaag cccagcagat ccactagttc tagagcggcc
     4381 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     4441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4621 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4741 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4801 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4861 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4921 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4981 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5041 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5101 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5161 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5221 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5281 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5341 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5401 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5461 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5521 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5581 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5641 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5701 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5761 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5821 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5881 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5941 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6001 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6061 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6121 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6181 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6241 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6301 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6361 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6421 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6481 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6541 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6601 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     6661 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     6721 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     6781 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cacgctggct aagaactcat
     6841 cagcctcccc ggtccatcta cccacgtacc aatgcaccaa ttggccacaa tgacggctac
     6901 tacatggtgc cattccttcc tctttatagg aatggagact acctcctgtc caacaaggct
     6961 cttggatacg agtacgccta cctgttggac ccaggtcatt gcacaacacc agaaatgccc
     7021 tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct gttccacaca
     7081 ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa aaattttact
     7141 caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc
     7201 tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa
     7261 ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct tttaatctca
     7321 aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt ttgtatttaa
     7381 ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag ttgtaaaacc
     7441 tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc ctagctcttt
     7501 tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc gatgcgggaa
     7561 gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc agagactccc
     7621 tggtgtctga aacacaggcc agatgggccc tcgag
//
