LOCUS       Exported                7530 bp ds-DNA     circular SYN 28-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..22
                     /label=M13R
     misc_recomb     41..160
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=97.5"
                     /note="/match_length=96.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     misc_feature    complement(201..506)
                     /label=ccdB
                     /label=nonstandard type:  Resistance
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /note="/codon_start=1"
                     /note="/translation=MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASA
                     RLLSD 
                     KVPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(826..1506)
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     misc_recomb     complement(1615..1738)
                     /label=attR3
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b; direction: LEFT"
     polyA_signal    complement(1778..1912)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        2047..2666
                     /label=crybb1 promoter (D. rerio)
                     /label=crybb1 promoter
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        2718..2874
                     /label=fragment of mimimal CMV promoter
                     /note="color: #a6acb3"
     CDS             2875..3573
                     /codon_start=1
                     /label=mKate2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_structure  complement(3580..3914)
                     /label=Tol2 exon1 bp1-335 of 461
                     /note="color: #a6acb3; direction: LEFT"
     LTR             complement(4077..4095)
                     /label=Tol2 5' TIR
     promoter        complement(4182..4200)
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(4216..4237)
                     /label=M13R
     rep_origin      complement(4623..5211)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             complement(5382..6242)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     LTR             complement(6984..7000)
                     /label=Tol2 3' TIR
     misc_structure  complement(7173..7519)
                     /label=Tol2 exon4 136-482
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgaac
       61 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      121 ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc agactggctg
      181 tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg
      241 tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac cggcacactg
      301 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt
      361 tcacggggga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc
      421 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt
      481 ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcgg caaaggagcc
      541 gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg
      601 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccgaac cggagatatt
      661 gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc
      721 tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta tatattctta
      781 taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc
      841 ctctagatta cgccccgcct gccactcatc gcagtactgt tgtaattcat taagcattct
      901 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
      961 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1021 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1081 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1141 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1201 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1261 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     1321 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1381 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1441 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1501 ctccatttta gcttccttag ctcctgaaaa tctcgacgga tcctaactca aaatccacac
     1561 attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg ccgccatagt
     1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1681 aatatattga tatttatatc attttacgtt tctcgttcaa ctttattata catagttgat
     1741 aattcactgg ccgtcgtttt acggtaccat cgatgatgat ccagacatga taagatacat
     1801 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1861 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1921 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     1981 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga tctcctcgag
     2041 caattggtgc tatcatcatg acaaagagag aagaaatcag ttattagaaa tgagttatta
     2101 aaactattat gattagaaat atgtttcttt ctcttaaaca ggatttaagg ggaaaatata
     2161 cagggagcga aaaattttga cttcatctgt atatacatat aataaataat acatcaataa
     2221 attccatcta aatacgctac catatatata tatatatgta tatacacaca cacacacaca
     2281 ccatctaata tatgtcatgc tacattatta acttcagtat gaaatctact ctgctctgga
     2341 gtgtatcagc gagcgactcc tctgctcgga ttatcatcac gtgacctctg acctgaacac
     2401 agacctctct tagccatagg tatacagcct atagcataga gctcgggctg agagggaaag
     2461 tgccgccgtc agggctttcc attgacagta aaatgcttac ggccttctgg gcgatgcgat
     2521 gaccctgcgc ttcggcacgg ctgaactctg atgacggcgt gtttctattg tccgccgcgc
     2581 gccgctgtgc tgcccttgcc agcgtataaa agctgcggca gtggtgagga aggccagagc
     2641 gagccagcct tcccagcaca gctgtagttt aaacgcgttg ccataacttc gtataggata
     2701 ctttatacga agttatctgc atgctaggtt gccgggaacg gtgcattgga acgcgcattc
     2761 cccgtgttaa ttaacaggta agtgtcttcc tcctgtttcc ttcccctgct attctgctca
     2821 accttcctat cagaaactgc agtatctgta tttttgctag gggatcccgc caccatggtg
     2881 agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac
     2941 caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
     3001 agaatcaagg cggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc
     3061 ttcatgtacg gcagcaaaac cttcatcaac cacacccagg gcatccccga cttctttaag
     3121 cagtccttcc ccgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
     3181 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
     3241 agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag
     3301 gcctccaccg agaccctgta ccccgctgac ggcggcctgg aaggcagagc cgacatggcc
     3361 ctgaagctcg tgggcggggg ccacctgatc tgcaacttga agaccacata cagatccaag
     3421 aaacccgcta agaacctcaa gatgcccggc gtctactatg tggacagaag actggaaaga
     3481 atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac
     3541 tgcgacctcc ctagcaaact ggggcacaga tgaagatctg cgaagatacg gccacgggtg
     3601 ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg aatcacatac
     3661 ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca ggtcaaggtg
     3721 ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc
     3781 tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa catttaaatt
     3841 aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat agaatgaagt
     3901 gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact
     3961 ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa
     4021 gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag
     4081 tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt
     4141 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     4201 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4441 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4501 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4561 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4621 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4681 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4741 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4801 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4861 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4921 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4981 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5041 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5101 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5161 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5221 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta aggcattttg
     5281 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5341 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5401 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5461 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5521 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5581 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5641 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5701 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5761 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5821 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5881 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5941 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6001 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6061 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6121 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6181 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6241 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6301 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6361 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     6421 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     6481 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     6541 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     6601 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     6661 ctaaagggag cccccgattt agagcttgac ggggaaaggc tggctaagaa ctcatcagcc
     6721 tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg gctactacat
     6781 ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca atgctcttgg
     6841 atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa tgccctctga
     6901 tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc acacaggtca
     6961 gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt
     7021 gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat ctggctagaa
     7081 tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt attaaggaag
     7141 taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa tctcaaaaaa
     7201 cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta tttaattatc
     7261 aaactataag acagacaatc taatgccagt acacgctact caaagttgta aaacctcaga
     7321 tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc tcttttgggg
     7381 ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc gggaagaggt
     7441 gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga ctccctggtg
     7501 tctgaaacac aggccagatg ggccctcgag
//
