LOCUS       Exported               10095 bp DNA     circular SYN 23-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   zack
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2022 from SnapGene 6.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10095
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     1..48
                     /label=ZEPCR#17
     misc_feature    1..30
                     /label=pAF
     misc_feature    1..30
                     /label=Site #1
     misc_feature    1..30
                     /label=pAF
     promoter        31..294
                     /standard_name="hU6"
                     /label=hU6 promoter
     primer_bind     complement(273..322)
                     /label=ZEPCR#18
     primer_bind     278..288
                     /label=LV5_R
     primer_bind     296..322
                     /label=ZEPCR#19
     misc_feature    296..301
                     /label=BsmBI
     terminator      302..337
                     /standard_name="bidirectional terminator, weaker in 
                     forwarddirection"
     CDS             complement(352..1029)
                     /standard_name="mRFP"
                     /label=mRFP
     misc_feature    complement(1030..1054)
                     /standard_name="RBS"
                     /label=RBS
     misc_feature    complement(1055..1108)
                     /standard_name="pTet"
                     /label=pTet
     primer_bind     complement(1087..1152)
                     /label=ZEPCR#20
     misc_feature    1109..1114
                     /label=BsmBI
     terminator      1116..1122
                     /standard_name="U6 terminator"
                     /label=U6 terminator
     primer_bind     1123..1164
                     /label=ZEPCR_22
     misc_feature    1123..1152
                     /label=gAR/pBF
     misc_feature    1123..1152
                     /label=Site #3
     misc_feature    1123..1152
                     /label=gAR/pBF
     promoter        1196..1407
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    1409..1438
                     /label=Site #4
     misc_feature    1409..1438
                     /label=Gibson gB-F
                     /note="Gibson gB-F"
     CDS             1445..1465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1466..2494
                     /codon_start=1
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    1466..2491
                     /label=Cre (BsmBI mutated)
     misc_feature    2140..2145
                     /label=mutated BsmBI site (originally CGTCTC)
     misc_feature    2495..2524
                     /label=Site #5
     misc_feature    2495..2524
                     /label=3' vect (Gibson gB-R)
     misc_feature    2580..3168
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2979..2998)
                     /label=LV5_R
     primer_bind     2979..2998
                     /label=LV6_F
     CDS             complement(3051..3062)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3240..3473
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3240..3294
                     /label=DeltaU3 (Trono)
     misc_feature    3240..3266
                     /label=attL (HIV U3 attachment site)
     misc_feature    3274..3275
                     /label=Insert heterologous sequences between these!
     misc_feature    3295..3390
                     /label=R
     misc_feature    3366..3371
                     /label=PolyA signal in R
     misc_feature    3391..3473
                     /label=U5
     misc_feature    3441..3473
                     /label=attR (HIV U5 attachment site)
     primer_bind     complement(3499..3516)
                     /label=QB0008
     polyA_signal    3505..3729
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3775..4203
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4217..4546
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4397..4532
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4594..4641
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4660..5034
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5164..5285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5334..5350)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5358..5374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5382..5412)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5427..5448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5736..6324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5974..5992
                     /label=LV8_F
     primer_bind     complement(5974..5992)
                     /label=LV7_R
     CDS             complement(6495..7355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6576..6599
                     /label=LV9_F
     primer_bind     complement(6576..6599)
                     /label=LV8_R
     primer_bind     7176..7196
                     /label=LV10_F
     primer_bind     complement(7176..7196)
                     /label=LV9_R
     promoter        complement(7356..7460)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7465..7488)
                     /label=LV10_R
     primer_bind     7490..7508
                     /label=LV1_F
     primer_bind     complement(7503..7516)
                     /label=LV1_F
     enhancer        7726..8105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(7955..7965)
                     /label=LV1_R
     primer_bind     complement(8068..8089)
                     /label=LV1_R
     primer_bind     8090..8113
                     /label=LV2_F
     promoter        8107..8305
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8119..8129)
                     /label=LV1_R
     LTR             8323..8503
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8325..8420
                     /label=R
     misc_feature    8421..8503
                     /label=U5
     misc_feature    8471..8503
                     /label=attR (HIV U5 attachment site)
     misc_feature    8505..8522
                     /label=PBS
     misc_feature    8550..8675
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8659..9023
                     /label=gag
                     /note="gag"
     primer_bind     8765..8791
                     /label=LV3_F
     primer_bind     complement(8765..8791)
                     /label=LV2_R
     misc_feature    9168..9401
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     9359..9382
                     /label=LV4_F
     primer_bind     complement(9359..9382)
                     /label=LV3_R
     misc_feature    9928..10045
                     /label=CPPT; central polypurine tract
                     /note="CPPT; central polypurine tract"
     primer_bind     9959..9980
                     /label=LV5_F
     primer_bind     complement(9959..9980)
                     /label=LV4_R
     primer_bind     complement(join(10086..10095,1..30))
                     /label=ZEPCR_21
ORIGIN
        1 gatcagtgtg agggagtgta aagctggttt aaggtcgggc aggaagaggg cctatttccc
       61 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
      121 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
      181 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
      241 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa cacccgagac
      301 ggtagtcaaa agcctccggt cggaggcttt tgacttggct gaggagtgcc attaagcacc
      361 ggtggagtga cgaccttcag cacgttcgta ctgttcaacg atggtgtagt cttcgttgtg
      421 ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc ggcagctgaa ccggtttttt
      481 agccatgtag gtggttttaa cttcagcgtc gtagtgacca ccgtctttca gtttcagacg
      541 cattttgatt tcacctttca gagcaccgtc ttccgggtac atacgttcgg tggaagcttc
      601 ccaacccatg gtttttttct gcataaccgg accgtcggac gggaagttgg taccacgcag
      661 tttaactttg tagatgaact caccgtcttg cagggaggag tcctgggtaa cggtaacaac
      721 accaccgtct tcgaagttca taacacgttc ccatttgaaa ccttccggga aggacagttt
      781 caggtagtcc gggatgtcag ccgggtgttt aacgtaagct ttggaaccgt actggaactg
      841 cggggacagg atgtcccaag cgaacggcag cggaccacct ttggtaactt tcagtttagc
      901 ggtctgggta ccttcgtacg gacgaccttc accttcacct tcgatttcga actcgtgacc
      961 gttaacggaa ccttccatac gaactttgaa acgcatgaac tctttgataa cgtcttcgct
     1021 actcgccata gatcctttct cctctttcag atccgtgctc agtatctcta tcactgatag
     1081 ggatgtcaat ctctatcact gatagggacg tctcgttttt ttaaaccgct gttcctagga
     1141 atcccgaggc cttaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
     1201 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt
     1261 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     1321 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     1381 cgcaacgggt ttgccgccag aacacaggga cccgacatta gcgctacagc ttaagcggac
     1441 catgcccaag aagaagagga aggtgtccaa tttactgacc gtacaccaaa atttgcctgc
     1501 attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga
     1561 tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc
     1621 ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga
     1681 ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg
     1741 ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt
     1801 ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca
     1861 ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga
     1921 tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg
     1981 tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat
     2041 gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact
     2101 tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtatctggtg tagctgatga
     2161 tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac
     2221 cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta
     2281 cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca gtgcccgtgt
     2341 cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg
     2401 tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg
     2461 ggcaatggtg cgcctgctgg aagatggcga ttagaaacgt tgttgtttgg ggttgaatta
     2521 ctctacatgt ttaagggttc cggttccact aggtacaatt cgatatcaag cttatcgata
     2581 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2641 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2701 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2761 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2821 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2881 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2941 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3001 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3061 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3121 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag
     3181 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     3241 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     3301 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     3361 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     3421 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     3481 gcgaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3541 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3601 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3661 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3721 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     3781 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3841 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3901 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3961 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4021 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4081 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4141 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4201 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4321 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4381 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4441 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4501 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     4561 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     4621 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     4681 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     4741 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     4801 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     4861 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     4921 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     4981 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     5041 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     5101 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     5161 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5221 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5281 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     5341 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5401 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5461 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5521 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5581 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5641 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6361 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6541 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6781 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6901 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7021 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7081 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7261 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7321 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     7501 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7561 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     7621 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     7681 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     7741 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     7801 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     7861 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     7921 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     7981 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     8041 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     8101 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     8161 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     8221 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     8281 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
     8341 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8401 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8461 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8521 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8581 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8641 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8701 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8761 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8821 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8881 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8941 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     9001 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     9061 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     9121 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     9181 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     9241 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     9301 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     9361 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     9421 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     9481 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     9541 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     9601 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     9661 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     9721 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     9781 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     9841 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     9901 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     9961 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
    10021 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
    10081 gttaattaag gtacc
//
