LOCUS       Exported               16693 bp ds-DNA     circular SYN 20-DEC-2022
DEFINITION  attB1-attB2.
ACCESSION   .
VERSION     .
KEYWORDS    pIF3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..16693
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          15..730
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          979..2926
                     /organism="PCR product"
                     /mol_type="other DNA"
     source          2927..2929
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2930..2932
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2936..2938
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2939..2941
                     /organism="recombinant plasmidffffff"
     source          2939..2941
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2942..2944
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2948..2950
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2948..2950
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2951..2953
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2960..2962
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2963..2965
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2969..2971
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          2972..2974
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          11789..11794
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      15..730
                     /label=Terminator_1
                     /note="Terminator_1"
                     /note="Trpc terminator color: #99ccff"
     misc_feature    363..364
                     /label=Misc_Feature_10
                     /note="Misc_Feature_10"
                     /note="BsaI? color: #a6acb3"
     misc_feature    882..905
                     /label=M13R(-48)
                     /note="M13R"
                     /note="M13R color: #3366cc"
     promoter        979..1344
                     /note="MoU3p"
     gene            979
                     /locus_tag="MGG_06216"
                     /label=MGG_06216
                     /note="MGG_06216"
                     /note="MGG_06216 color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:2684344"
     mRNA            979
                     /locus_tag="MGG_06216"
                     /product="hypothetical protein"
                     /label=mRNA_1
                     /note="mRNA_1"
                     /note="MGG_06216 mRNA color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:2684344"
     gene            1343..1344
                     /note="Fungi_U3 color: #00ff00; direction: RIGHT"
     promoter        1421..1787
                     /note="gpd promoter"
     CDS             1799..2926
                     /codon_start=1
                     /label=HSVtk
                     /note="HSVtk"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     misc_feature    2927..2986
                     /label=Misc_Feature_2
                     /note="Misc_Feature_2"
                     /note="T2A color: #00ff00"
     misc_feature    2987..5059
                     /label=HPTBOX
                     /note="HPTBOX"
     misc_feature    5060..5066
                     /label=nt.bbvci
                     /note="nt.bbvci"
                     /note="nt.bbvci color: #3366cc"
     misc_feature    5065..5071
                     /label=dn-s
                     /note="dn-s"
                     /note="dn-s color: #3366cc"
     misc_feature    5076..5092
                     /label=M13F(-20)
                     /note="M13F"
     misc_feature    5076..5092
                     /label=M13F(-20)
                     /note="M13F"
                     /note="M13F color: #3366cc"
     misc_feature    5314..5339
                     /label=Misc_Feature_2
                     /note="right border T-DNA repeat"
     misc_feature    5314..5339
                     /label=Misc_Feature_2
                     /note="Misc_Feature_2"
                     /note="right border T-DNA repeat color: #3366cc"
     misc_feature    complement(6380..7380)
                     /label=Misc_Feature_3
                     /note="STA region from pVS1 plasmid"
     misc_feature    complement(6380..7380)
                     /label=Misc_Feature_3
                     /note="Misc_Feature_3"
                     /note="STA region from pVS1 plasmid color: #66cc99; 
                     direction: LEFT"
     rep_origin      complement(7973..8973)
                     /direction=LEFT
                     /label=Rep_Origin_1
                     /note="Rep_Origin_1"
                     /note="pVS1-REP; replication origin from pVS1 color: 
                     #ff9966"
     misc_feature    complement(9383..9643)
                     /label=Misc_Feature_4
                     /note="bom site from pBR322"
     rep_origin      complement(9783..10063)
                     /direction=LEFT
                     /label=Rep_Origin_2
                     /note="Rep_Origin_2"
                     /note="pBR322 origin of replication color: #ff9966"
     CDS             complement(10354..11148)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=CDS_2
                     /note="Kan resistance gene"
                     /note="aadA (kanamycin resistance) gene amplified from 
                     pIG121Hm"
                     /db_xref="GI:7638067"
                     /protein_id="AAF65338.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11573..11598
                     /label=Misc_Feature_5
                     /note="left border repeat from C58 T-DNA"
     misc_feature    11665..11788
                     /label=Misc_Feature_6
                     /note="CaMV 3'UTR (polyA signal)"
     misc_feature    11789
                     /label=Misc_Feature_8
                     /note="Misc_Feature_8"
                     /note="MCS color: #99ccff"
     CDS             11789
                     /codon_start=1
                     /label=CDS_3
                     /note="CDS_3"
                     /note="lacZ-alpha color: #993366"
                     /translation=""
     exon            11795..12272
                     /label=RP27
                     /note="RP27"
     misc_feature    12274..12294
                     /label=attB1
                     /note="attB1"
     misc_feature    12300..12304
                     /label=EcoRIHS
                     /note="EcoRIHS"
                     /note="EcoRIHS color: #a6acb3"
     misc_feature    12321..12347
                     /label=HA-Tag
                     /note="HA-Tag"
                     /note="HA-Tag color: #a6acb3"
     misc_feature    12351..12371
                     /label=NLS
                     /note="NLS"
                     /note="NLS color: #a6acb3"
     misc_feature    join(12381..14894,14898..16481)
                     /label=hSpCsn1
                     /note="hSpCsn1"
                     /note="hSpCsn1 This feature has 2 segments: 
                     ???1:??752?..?3265?/?#a6acb3 ???2:?3269?..?4852?/?#a6acb3"
     misc_feature    12405..12407
                     /label=pUC ori
                     /note="pUC ori"
                     /note="pUC ori D10A color: #a6acb3"
     misc_feature    12405..12407
                     /label=(GAC)D10A(GCC)
     misc_feature    14895..14897
                     /label=(CAT)H840A(GCC)
     misc_feature    16524
                     /label=Misc_Feature_1
                     /note="Misc_Feature_1"
     CDS             16530..16652
                     /codon_start=1
                     /label=CDS_1
                     /note="CDS_1"
                     /note="Mxi1 color: #808000"
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     misc_feature    16661..16664
                     /label=Misc_Feature_2
                     /note="Misc_Feature_2"
     misc_feature    join(16686..16693,1..13)
                     /label=attB2
                     /note="attB2"
ORIGIN
        1 ttgtacaaag tggtacttaa cgttactgaa atcatcaaac agcttgacga atctggatat
       61 aagatcgttg gtgtcgatgt cagctccgga gttgagacaa atggtgttca ggatctcgat
      121 aagatacgtt catttgtcca agcagcaaag agtgccttct agtgatttaa tagctccatg
      181 tcaacaagaa taaaacgcgt ttcgggttta cctcttccag atacagctca tctgcaatgc
      241 attaatgcat tggacctcgc aaccctagta cgcccttcag gctccggcga agcagaagaa
      301 tagcttagca gagtctattt tcattttcgg gagacgagat caagcagatc aacggtcgtc
      361 aatggaccta cgagactgag gaatccgctc ttggctccac gcgactatat atttgtctct
      421 aattgtactt tgacatgctc ctcttcttta ctctgatagc ttgactatga aaattccgtc
      481 accagcccct gggttcgcaa agataattgc actgtttctt ccttgaactc tcaagcctac
      541 aggacacaca ttcatcgtag gtataaacct cgaaaatcat tcctactaag atgggtatac
      601 aatagtaacc atggttgcct agtgaatgct ccgtaacacc caatacgccg gccgaaactt
      661 ttttacaact ctcctatgag tcgtttaccc agaatgcaca ggtacacttg tttagaggta
      721 atccttcttt ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      781 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      841 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      901 caggaaacag ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctctagag
      961 tcgacctgca ggcatgcaca ctacgcgaat catgtctctg agagtattct agctgattgg
     1021 cgaatgtcta agcaaatgga tgcagagaac cactcgatct tcgtgtctca atcataggcg
     1081 gaattagatt cccatataaa gagaatgctg ccccacaatg ggcccgccca acggcaaagg
     1141 atgtgggtgt gtattagtca gctgcaaact tttcttgcac aggccagaca gttaccccac
     1201 ttgtagagag tataagaata cctgctcgcc ctctccgaat cgtttttgtg tttctctttc
     1261 taccatgtcg atcttacttc aaagtgagtc tcccatacac agcctcttca cctctccagc
     1321 ctttccaagc aacaccatca ctaaggagac cgaggtctcg gttttgaatt gggtactcaa
     1381 attggttccc gcttgacgac attccgaaac ccccaatttc gcggcttcga atcgtggcta
     1441 cccttggccg aggttgcgat ttctctgccg tatctgacaa tgatccaacc ggccggatgt
     1501 gtgggagggc gcaaagggtg taattgggtc taggcgtgag ctgcatgtcc gatggcagaa
     1561 aaagaactac taaaactctg tgacgtcctc agggctggta gctatttagc ttgcccctcc
     1621 ctcaccccac ctagctcaaa cccaacaaaa cacagcaccg accacaaaaa tctgcctctt
     1681 accacctgct catcaccttt tctcacatat aaagcagtga gctcagcccg atttccattc
     1741 ctcaattcaa gtctattaac tctctcaaag aggaacccaa tcttcaaagg atcgatccat
     1801 ggcttcgtac ccctgccatc aacacgcgtc tgcgttcgac caggctgcgc gttctcgcgg
     1861 ccatagcaac cgacgtacgg cgttgcgccc tcgccggcag caagaagcca cggaagtccg
     1921 cctggagcag aaaatgccca cgctactgcg ggtttatata gacggtcctc acgggatggg
     1981 gaaaaccacc accacgcaac tgctggtggc cctgggttcg cgcgacgaca tcgtctacgt
     2041 acccgagccg atgacttact ggcaggtgct gggggcttcc gagacaatcg cgaacatcta
     2101 caccacacaa caccgcctcg accagggtga gatttcggcc ggggacgcgg cggtggtaat
     2161 gacaagcgcc cagataacaa tgggcatgcc ttatgccgtg accgacgccg ttctggctcc
     2221 tcatatcggg ggggaggctg gcagctcaca tgccccgccc ccggccctca ccctcatctt
     2281 cgaccgccat cccatcgccg ccctcctgtg ctacccggcc gcgcgatacc ttatgggcag
     2341 catgaccccc caggccgtgc tggcgttcgt ggccctcatc ccgccgacct tgcccggcac
     2401 aaacatcgtg ttgggggccc ttccggagga cagacacatc gaccgcctgg ccaaacgcca
     2461 gcgccccggc gagcggcttg acctggctat gctggccgcg attcgccgcg tttacgggct
     2521 gcttgccaat acggtgcggt atctccaggg cggcgggtcg tggcgggagg attggggaca
     2581 gctttcgggg acggccgtgc cgccccaggg tgccgagccc cagagcaacg cgggcccacg
     2641 accccatatc ggggacacgt tatttaccct gtttcgggcc cccgagttgc tggcccccaa
     2701 cggcgacctg tacaacgtgt ttgcctgggc cttggacgtc ttggccaaac gcctccgtcc
     2761 catgcacgtc tttatcctgg attacgacca atcgcccgcc ggctgccggg acgccctgct
     2821 gcaacttacc tccgggatgg tccagaccca cgtcaccacc cccggctcca taccgacgat
     2881 ctgcgacctg gcgcgcacgt ttgcccgcga gatgggggag gctaaccgtg ccgagggtcg
     2941 tggttcgctc ctcacctgtg gtgacgtcga ggagaaccct ggtcctatga aaaagcctga
     3001 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     3061 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     3121 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     3181 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     3241 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     3301 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     3361 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3421 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     3481 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     3541 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     3601 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     3661 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     3721 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     3781 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     3841 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     3901 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     3961 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg
     4021 ccgaccggga tccacttaac gttactgaaa tcatcaaaca gcttgacgaa tctggatata
     4081 agatcgttgg tgtcgatgtc agctccggag ttgagacaaa tggtgttcag gatctcgata
     4141 agatacgttc atttgtccaa gcagcaaaga gtgccttcta gtgatttaat agctccatgt
     4201 caacaagaat aaaacgcgtt tcgggtttac ctcttccaga tacagctcat ctgcaatgca
     4261 ttaatgcatt ggacctcgca accctagtac gcccttcagg ctccggcgaa gcagaagaat
     4321 agcttagcag agtctatttt cattttcggg agacgagatc aagcagatca acggtcgtca
     4381 atggacctac gagactgagg aatccgctct tggctccacg cgactatata tttgtctcta
     4441 attgtacttt gacatgctcc tcttctttac tctgatagct tgactatgaa aattccgtca
     4501 ccagcccctg ggttcgcaaa gataattgca ctgtttcttc cttgaactct caagcctaca
     4561 ggacacacat tcatcgtagg tataaacctc gaaaatcatt cctactaaga tgggtataca
     4621 atagtaacca tggttgccta gtgaatgctc cgtaacaccc aatacgccgg ccgaaacttt
     4681 tttacaactc tcctatgagt cgtttaccca gaatgcacag gtacacttgt ttagaggtaa
     4741 tccttctttc tagaggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     4801 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     4861 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     4921 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     4981 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagatct
     5041 agaggatccc ccgactagtg ctgaggcatt atggcactgg ccgtcgtttt acaacgtcgt
     5101 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     5161 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     5221 aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt
     5281 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     5341 tagaataacg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     5401 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
     5461 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
     5521 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     5581 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     5641 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     5701 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     5761 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     5821 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     5881 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     5941 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     6001 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     6061 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     6121 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     6181 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     6241 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     6301 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     6361 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     6421 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     6481 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     6541 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     6601 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     6661 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     6721 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     6781 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     6841 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     6901 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     6961 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     7021 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     7081 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     7141 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     7201 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     7261 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     7321 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     7381 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     7441 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     7501 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     7561 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     7621 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     7681 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     7741 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     7801 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     7861 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     7921 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     7981 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     8041 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     8101 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     8161 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     8221 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     8281 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     8341 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     8401 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     8461 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     8521 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     8581 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     8641 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     8701 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     8761 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaagcactct ttcctgtgga
     8821 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     8881 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     8941 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     9001 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     9061 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     9121 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     9181 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     9241 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     9301 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     9361 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     9421 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     9481 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9541 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9601 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9841 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10081 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    10141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10201 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10261 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10321 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
    10381 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
    10441 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
    10501 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
    10561 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
    10621 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
    10681 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
    10741 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
    10801 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
    10861 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
    10921 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
    10981 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
    11041 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
    11101 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
    11161 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
    11221 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
    11281 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
    11341 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
    11401 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
    11461 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
    11521 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
    11581 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
    11641 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg
    11701 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
    11761 ggtttcttat atgctcaaca catgagcgga attcataaat gtaggtatta cctgtacatt
    11821 ttatttattc gagaaaaaca aacaaaccaa aacgatgcag cgatcactac cggcgtcatc
    11881 cggaattgaa acggcgcgcg ttgagggggg ccttcttgtt cggacacaca cggggccgcc
    11941 aaagtggggc ccatacggct gcccatccct tgctctccaa taccctggtc atgtgactcg
    12001 aaattggaga ctcacaaaga tgctttgaaa ccccgccggg aactttttct cacagacaca
    12061 atcagtcgac cttatcatcg caaaatcgac aaacctcaaa aagaactcga agagtcagtc
    12121 tcccttgcgg ttaaactgat tgatatctcg tgttctctaa accttcaata agtgagttac
    12181 tgcgcacaaa gccgactata ctgagcacca acgcgaattg ctcacgttgt cgcctaacag
    12241 atcttggctt tcgtaggaac ccaatcttca aaacaagttt gtacaaaaaa gcaggctccg
    12301 aattcaccgg tgccaccatg tacccatacg atgttccaga ttacgcttcg ccgaagaaaa
    12361 agcgcaaggt cgaagcgtcc gacaagaagt acagcatcgg cctggccatc ggcaccaact
    12421 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
    12481 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
    12541 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
    12601 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
    12661 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
    12721 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
    12781 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
    12841 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
    12901 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
    12961 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
    13021 gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
    13081 atggcctgtt cggcaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
    13141 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
    13201 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
    13261 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
    13321 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
    13381 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
    13441 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
    13501 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca
    13561 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
    13621 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
    13681 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
    13741 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
    13801 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
    13861 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
    13921 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
    13981 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
    14041 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
    14101 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
    14161 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
    14221 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
    14281 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
    14341 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
    14401 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
    14461 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
    14521 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
    14581 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
    14641 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
    14701 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
    14761 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
    14821 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
    14881 actacgatgt ggacgccatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
    14941 aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
    15001 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
    15061 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
    15121 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
    15181 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
    15241 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
    15301 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
    15361 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
    15421 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
    15481 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
    15541 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
    15601 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
    15661 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
    15721 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
    15781 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
    15841 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
    15901 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
    15961 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
    16021 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
    16081 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
    16141 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
    16201 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
    16261 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
    16321 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
    16381 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
    16441 tgtacgagac acggatcgac ctgtctcagc tgggaggcga cagccccaag aagaagagaa
    16501 aggtggaggc cagcgggccg gccggatcca tggaacgtgt gagaatgatt aatgtgcaaa
    16561 ggctgttaga agccgcagag tttttagaaa gaagagaaag agaatgcgaa cacgggtatg
    16621 ccagttcttt ccctagcatg ccctctccca gaggctaaat cgattgatta attaagaatt
    16681 cgacccagct ttc
//
