LOCUS       Exported               16817 bp ds-DNA     circular SYN 20-DEC-2022
DEFINITION  attB1-attB2.
ACCESSION   .
VERSION     .
KEYWORDS    pIF4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..16817
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          15..730
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          979..1464
                     /organism="PCR product"
                     /mol_type="other DNA"
     source          1469..3050
                     /organism="PCR product"
                     /mol_type="other DNA"
     source          3051..3053
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          3054..3056
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          3060..3062
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          3063..3065
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          join(3066..16817,1)
     source          3066..3068
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          3072..3074
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          3072..3074
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          3075..3077
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          3084..3086
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          3087..3089
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          3093..3095
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          3096..3098
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          11913..11918
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1
                     /label=T2A color: #00ff00
                     /note="T2A color: #00ff00"
     terminator      15..730
                     /label=Terminator_1
                     /note="Terminator_1"
                     /note="""""""""Trpc terminator colo;Terminator_1;Trpc 
                     terminator color: #99ccff"
     misc_feature    363..364
                     /label=Misc_Feature_14
                     /note="Misc_Feature_14"
                     /note="BsaI? color: #a6acb3"
     promoter        831..885
                     /label=Promoter_P_2
                     /note="Promoter_P_2"
                     /note="LacZ promoter PlacZ; lacZ promoter??color: #800000; 
                     direction: RIGHT"
     misc_feature    882..905
                     /label=M13R(-48)
                     /note="M13R"
                     /note="M13R color: #3366cc"
     promoter        979..1464
                     /label=Promoter_P_2
                     /note="U6 promoter"
                     /note="U6 promoter color: #00ffff; direction: RIGHT"
     misc_feature    1328..1329
                     /label=Misc_Feature_2
                     /note="Misc_Feature_2"
                     /note="TC-at color: #a6acb3"
     misc_feature    1465..1468
                     /label=Feature 64
                     /note="Feature 64"
     promoter        1545..1911
                     /label=Promoter_P_3
                     /note="GPD promoter"
                     /note="Promoter_P_3;gpd promoter color: #99cc00; direction:
                     RIGHT"
     misc_feature    1797
                     /label=Misc_Feature_3
                     /note="Misc_Feature_3"
                     /note="pEASY ?-lambda- color: #a6acb3"
     CDS             1923..3050
                     /codon_start=1
                     /label=HSVtk
                     /note="HSVtk"
                     /note="HSVtk ????????color: #ff9966"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     misc_feature    2229
                     /label=Misc_Feature_4
                     /note="Misc_Feature_4"
                     /note="pEASY ? ?-lambda- color: #a6acb3"
     misc_feature    2549
                     /label=Misc_Feature_5
                     /note="Misc_Feature_5"
                     /note="pEASY-blunt ?? g-a color: #ff0000"
     misc_feature    3051..3110
                     /label=Misc_Feature_6
                     /note="Misc_Feature_6"
                     /note="T2A color: #00ff00"
     misc_feature    3111..5183
                     /label=HPTBOX
                     /note="HPTBOX"
                     /note="HPTBOX color: #66cc99; direction: RIGHT"
     misc_feature    3111..4136
                     /label=HPT CDS
                     /note="HPT CDS"
                     /note="HPT CDS color: #66cc99; direction: RIGHT"
     misc_feature    5184..5190
                     /label=nt.bbvci
                     /note="nt.bbvci"
                     /note="nt.bbvci color: #3366cc"
     misc_feature    5189..5195
                     /label=dn-s
                     /note="dn-s"
                     /note="dn-s color: #3366cc"
     misc_feature    5200..5216
                     /label=M13F(-20)
                     /note="M13F"
                     /note="M13F color: #3366cc"
     misc_feature    5438..5463
                     /label=Misc_Feature_2
                     /note="Misc_Feature_2"
                     /note="right border T-DNA repeat color: #3366cc"
     misc_feature    complement(6504..7504)
                     /label=Misc_Feature_3
                     /note="Misc_Feature_3"
                     /note="STA region from pVS1 plasmid color: #66cc99; 
                     direction: LEFT"
     rep_origin      complement(8097..9097)
                     /direction=LEFT
                     /label=Rep_Origin_1
                     /note="Rep_Origin_1"
                     /note="pVS1-REP; replication origin from pVS1 color: 
                     #ff9966"
     misc_feature    complement(9507..9767)
                     /label=Misc_Feature_4
                     /note="Misc_Feature_4"
                     /note="bom site from pBR322 color: #66cc99; direction: 
                     LEFT"" pBR322 color: #66cc99; direction: LEFT"
     rep_origin      complement(9907..10187)
                     /direction=LEFT
                     /label=Rep_Origin_2
                     /note="Rep_Origin_2"
                     /note="pBR322 origin of replication color: #ff9966"
     CDS             complement(10478..11272)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=CDS_2
                     /note="CDS_2"
                     /note="Kan resistance gene aadA (kanamycin resistance) gene
                     amplified from??pIG121Hm color: #ff9966"
                     /db_xref="GI:7638067"
                     /protein_id="AAF65338.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11697..11722
                     /label=Misc_Feature_5
                     /note="Misc_Feature_5"
                     /note="left border repeat from C58 T-DNA color: #3366cc"
     misc_feature    11789..11912
                     /label=Misc_Feature_6
                     /note="Misc_Feature_6"
                     /note="CaMV 3'UTR (polyA signal) color: #66cc99; direction:
                     RIGHT"
     CDS             11913
                     /codon_start=1
                     /label=CDS_3
                     /note="CDS_3"
                     /note="lacZ-alpha color: #993366"
                     /translation=""
     misc_feature    11913
                     /label=Misc_Feature_12
                     /note="Misc_Feature_12"
                     /note="MCS color: #99ccff"
     exon            11919..12396
                     /label=RP27
                     /note="RP27"
                     /note="RP27 color: #ff6600; direction: RIGHT"
     misc_feature    12398..12418
                     /label=attB1
                     /note="attB1"
     misc_feature    12424..12428
                     /label=EcoRIHS
                     /note="EcoRIHS"
                     /note="EcoRIHS color: #a6acb3"
     misc_feature    12445..12471
                     /label=HA-Tag
                     /note="HA-Tag"
                     /note="HA-Tag color: #a6acb3"
     misc_feature    12475..12495
                     /label=NLS
                     /note="NLS"
                     /note="NLS color: #a6acb3"
     misc_feature    join(12505..15018,15022..16605)
                     /label=hSpCsn1
                     /note="hSpCsn1"
                     /note="hSpCsn1 This feature has 2 segments: 
                     ???1:??752?..?3265?/?#a6acb3 ???2:?3269?..?4852?/?#a6acb3"
     misc_feature    12529..12531
                     /label=(GAC)D10A(GCC)
     misc_feature    12529..12531
                     /label=pUC ori
                     /note="pUC ori"
                     /note="pUC ori D10A??color: #a6acb3"
     misc_feature    15019..15021
                     /label=(CAT)H840A(GCC)
     misc_feature    16648
                     /label=VP160
                     /note="VP160"
                     /note="VP160 color: #a6acb3"
     misc_feature    16648
                     /label=Misc_Feature_1
                     /note="Misc_Feature_1"
     CDS             16654..16776
                     /codon_start=1
                     /label=CDS_1
                     /note="Mxi1"
                     /note="Mxi1 color: #808000"
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     misc_feature    16785..16788
                     /label=VP160
                     /note="VP160"
                     /note="VP160 color: #a6acb3"
     misc_feature    16785..16788
                     /label=Misc_Feature_2
                     /note="Misc_Feature_2"
     misc_feature    join(16810..16817,1..13)
                     /label=attB2
                     /note="attB2"
ORIGIN
        1 ttgtacaaag tggtacttaa cgttactgaa atcatcaaac agcttgacga atctggatat
       61 aagatcgttg gtgtcgatgt cagctccgga gttgagacaa atggtgttca ggatctcgat
      121 aagatacgtt catttgtcca agcagcaaag agtgccttct agtgatttaa tagctccatg
      181 tcaacaagaa taaaacgcgt ttcgggttta cctcttccag atacagctca tctgcaatgc
      241 attaatgcat tggacctcgc aaccctagta cgcccttcag gctccggcga agcagaagaa
      301 tagcttagca gagtctattt tcattttcgg gagacgagat caagcagatc aacggtcgtc
      361 aatggaccta cgagactgag gaatccgctc ttggctccac gcgactatat atttgtctct
      421 aattgtactt tgacatgctc ctcttcttta ctctgatagc ttgactatga aaattccgtc
      481 accagcccct gggttcgcaa agataattgc actgtttctt ccttgaactc tcaagcctac
      541 aggacacaca ttcatcgtag gtataaacct cgaaaatcat tcctactaag atgggtatac
      601 aatagtaacc atggttgcct agtgaatgct ccgtaacacc caatacgccg gccgaaactt
      661 ttttacaact ctcctatgag tcgtttaccc agaatgcaca ggtacacttg tttagaggta
      721 atccttcttt ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      781 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      841 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      901 caggaaacag ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctctagag
      961 tcgacctgca ggcatgcata ggctgaggct cacgccgttg agcctgcgcg ataacaacaa
     1021 cacgaacgaa ggtcgggcca tgtcggaata tgtgacgccg ccgatgatgg cgggtgaaag
     1081 agattctgcg gcttgagggg tcgcgacatg ggagttttcg tcgggtcatc ctttggttta
     1141 cagcagcaaa ccattccact ttatgaagtg tattttgaag gttcaagagg gcttcttaat
     1201 ctccggtcgg gagagttatt atgacactca ctgcgaaagg atgccaaggt atctatttgt
     1261 ggcgatttcc caactcacca tacccttggg ttgctaatgt gcctggaaag gaagggtcca
     1321 gggatggatt cgcaatcatc ggttgacagc tcaggatcag caagaaagcc agtgctgcca
     1381 gaggtctgtg ctgttggcaa atgtgcagag gagagggaca cggccgtcca aggtacataa
     1441 gtcactttgt tttggacttc tttcctaagg agaccgaggt ctcggttttg aattgggtac
     1501 tcaaattggt tcccgcttga cgacattccg aaacccccaa tttcgcggct tcgaatcgtg
     1561 gctacccttg gccgaggttg cgatttctct gccgtatctg acaatgatcc aaccggccgg
     1621 atgtgtggga gggcgcaaag ggtgtaattg ggtctaggcg tgagctgcat gtccgatggc
     1681 agaaaaagaa ctactaaaac tctgtgacgt cctcagggct ggtagctatt tagcttgccc
     1741 ctccctcacc ccacctagct caaacccaac aaaacacagc accgaccaca aaaatctgcc
     1801 tcttaccacc tgctcatcac cttttctcac atataaagca gtgagctcag cccgatttcc
     1861 attcctcaat tcaagtctat taactctctc aaagaggaac ccaatcttca aaggatcgat
     1921 ccatggcttc gtacccctgc catcaacacg cgtctgcgtt cgaccaggct gcgcgttctc
     1981 gcggccatag caaccgacgt acggcgttgc gccctcgccg gcagcaagaa gccacggaag
     2041 tccgcctgga gcagaaaatg cccacgctac tgcgggttta tatagacggt cctcacggga
     2101 tggggaaaac caccaccacg caactgctgg tggccctggg ttcgcgcgac gacatcgtct
     2161 acgtacccga gccgatgact tactggcagg tgctgggggc ttccgagaca atcgcgaaca
     2221 tctacaccac acaacaccgc ctcgaccagg gtgagatttc ggccggggac gcggcggtgg
     2281 taatgacaag cgcccagata acaatgggca tgccttatgc cgtgaccgac gccgttctgg
     2341 ctcctcatat cgggggggag gctggcagct cacatgcccc gcccccggcc ctcaccctca
     2401 tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc ggccgcgcga taccttatgg
     2461 gcagcatgac cccccaggcc gtgctggcgt tcgtggccct catcccgccg accttgcccg
     2521 gcacaaacat cgtgttgggg gcccttccgg aggacagaca catcgaccgc ctggccaaac
     2581 gccagcgccc cggcgagcgg cttgacctgg ctatgctggc cgcgattcgc cgcgtttacg
     2641 ggctgcttgc caatacggtg cggtatctcc agggcggcgg gtcgtggcgg gaggattggg
     2701 gacagctttc ggggacggcc gtgccgcccc agggtgccga gccccagagc aacgcgggcc
     2761 cacgacccca tatcggggac acgttattta ccctgtttcg ggcccccgag ttgctggccc
     2821 ccaacggcga cctgtacaac gtgtttgcct gggccttgga cgtcttggcc aaacgcctcc
     2881 gtcccatgca cgtctttatc ctggattacg accaatcgcc cgccggctgc cgggacgccc
     2941 tgctgcaact tacctccggg atggtccaga cccacgtcac cacccccggc tccataccga
     3001 cgatctgcga cctggcgcgc acgtttgccc gcgagatggg ggaggctaac cgtgccgagg
     3061 gtcgtggttc gctcctcacc tgtggtgacg tcgaggagaa ccctggtcct atgaaaaagc
     3121 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     3181 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     3241 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     3301 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     3361 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     3421 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     3481 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     3541 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     3601 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     3661 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     3721 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     3781 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     3841 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     3901 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     3961 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     4021 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     4081 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta
     4141 gatgccgacc gggatccact taacgttact gaaatcatca aacagcttga cgaatctgga
     4201 tataagatcg ttggtgtcga tgtcagctcc ggagttgaga caaatggtgt tcaggatctc
     4261 gataagatac gttcatttgt ccaagcagca aagagtgcct tctagtgatt taatagctcc
     4321 atgtcaacaa gaataaaacg cgtttcgggt ttacctcttc cagatacagc tcatctgcaa
     4381 tgcattaatg cattggacct cgcaacccta gtacgccctt caggctccgg cgaagcagaa
     4441 gaatagctta gcagagtcta ttttcatttt cgggagacga gatcaagcag atcaacggtc
     4501 gtcaatggac ctacgagact gaggaatccg ctcttggctc cacgcgacta tatatttgtc
     4561 tctaattgta ctttgacatg ctcctcttct ttactctgat agcttgacta tgaaaattcc
     4621 gtcaccagcc cctgggttcg caaagataat tgcactgttt cttccttgaa ctctcaagcc
     4681 tacaggacac acattcatcg taggtataaa cctcgaaaat cattcctact aagatgggta
     4741 tacaatagta accatggttg cctagtgaat gctccgtaac acccaatacg ccggccgaaa
     4801 cttttttaca actctcctat gagtcgttta cccagaatgc acaggtacac ttgtttagag
     4861 gtaatccttc tttctagagg atcctctacg ccggacgcat cgtggccggc atcaccggcg
     4921 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     4981 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     5041 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     5101 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag
     5161 atctagagga tcccccgact agtgctgagg cattatggca ctggccgtcg ttttacaacg
     5221 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5281 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5341 cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca
     5401 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     5461 ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     5521 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
     5581 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
     5641 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     5701 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     5761 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     5821 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     5881 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     5941 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     6001 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     6061 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     6121 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
     6181 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     6241 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     6301 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     6361 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     6421 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     6481 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     6541 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     6601 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     6661 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     6721 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     6781 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     6841 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     6901 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     6961 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     7021 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     7081 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     7141 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     7201 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     7261 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     7321 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     7381 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     7441 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     7501 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     7561 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     7621 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     7681 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     7741 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     7801 gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     7861 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     7921 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     7981 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     8041 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     8101 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     8161 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     8221 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     8281 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     8341 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     8401 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     8461 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     8521 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     8581 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     8641 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     8701 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     8761 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     8821 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     8881 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaagca ctctttcctg
     8941 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     9001 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     9061 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     9121 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     9181 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     9241 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     9301 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
     9361 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     9421 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     9481 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     9541 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     9601 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    10021 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    10081 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    10141 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    10201 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    10261 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    10321 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    10381 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    10441 actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa
    10501 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
    10561 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
    10621 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
    10681 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
    10741 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
    10801 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
    10861 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
    10921 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
    10981 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
    11041 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
    11101 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
    11161 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
    11221 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
    11281 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
    11341 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
    11401 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg
    11461 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
    11521 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
    11581 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
    11641 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc
    11701 aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg
    11761 tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat
    11821 tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac
    11881 taagggtttc ttatatgctc aacacatgag cggaattcat aaatgtaggt attacctgta
    11941 cattttattt attcgagaaa aacaaacaaa ccaaaacgat gcagcgatca ctaccggcgt
    12001 catccggaat tgaaacggcg cgcgttgagg ggggccttct tgttcggaca cacacggggc
    12061 cgccaaagtg gggcccatac ggctgcccat cccttgctct ccaataccct ggtcatgtga
    12121 ctcgaaattg gagactcaca aagatgcttt gaaaccccgc cgggaacttt ttctcacaga
    12181 cacaatcagt cgaccttatc atcgcaaaat cgacaaacct caaaaagaac tcgaagagtc
    12241 agtctccctt gcggttaaac tgattgatat ctcgtgttct ctaaaccttc aataagtgag
    12301 ttactgcgca caaagccgac tatactgagc accaacgcga attgctcacg ttgtcgccta
    12361 acagatcttg gctttcgtag gaacccaatc ttcaaaacaa gtttgtacaa aaaagcaggc
    12421 tccgaattca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
    12481 aaaaagcgca aggtcgaagc gtccgacaag aagtacagca tcggcctggc catcggcacc
    12541 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
    12601 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
    12661 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
    12721 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
    12781 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
    12841 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
    12901 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
    12961 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
    13021 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
    13081 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
    13141 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
    13201 aagaatggcc tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
    13261 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
    13321 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
    13381 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
    13441 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
    13501 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
    13561 cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga agagttctac
    13621 aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg
    13681 aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag
    13741 atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg
    13801 aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc
    13861 cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc
    13921 accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag
    13981 cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg
    14041 ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga
    14101 atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc
    14161 aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag
    14221 tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca
    14281 taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag
    14341 gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag
    14401 gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg
    14461 cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag
    14521 cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc
    14581 atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg
    14641 tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt
    14701 aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg
    14761 cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga
    14821 cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc
    14881 cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg
    14941 tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg
    15001 tccgactacg atgtggacgc catcgtgcct cagagctttc tgaaggacga ctccatcgac
    15061 aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa
    15121 gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc
    15181 cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag
    15241 gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag
    15301 atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg
    15361 aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac
    15421 aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg
    15481 ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac
    15541 aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc
    15601 gccaagtact tcttctacag caacatcatg aactttttca agaccgagat taccctggcc
    15661 aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg
    15721 tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat
    15781 atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag
    15841 aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc
    15901 ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag
    15961 tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc
    16021 ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac
    16081 ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg
    16141 ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg
    16201 aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag
    16261 cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc
    16321 agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc
    16381 tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt
    16441 accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg
    16501 aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc
    16561 ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacagccc caagaagaag
    16621 agaaaggtgg aggccagcgg gccggccgga tccatggaac gtgtgagaat gattaatgtg
    16681 caaaggctgt tagaagccgc agagttttta gaaagaagag aaagagaatg cgaacacggg
    16741 tatgccagtt ctttccctag catgccctct cccagaggct aaatcgattg attaattaag
    16801 aattcgaccc agctttc
//
