LOCUS       Exported               10298 bp ds-DNA     circular SYN 13-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Kazuhiko
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 20, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..19)
                     /direction=LEFT
                     /label=Deleated f1 ori
                     /note="f1 ori;f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis;Geneious name: 
                     f1 ori rep origin"
     misc_feature    30..180
                     /label=Tolmini-L
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    327..461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        466..483
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(504..1370)
                     /codon_start=1
                     /product="zebrafish codon optimized human HO1"
                     /label=zHO1
                     /translation="MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRD
                     GFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEV
                     IPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEG
                     LAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLT
                     HDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATV
                     AVGLYAM"
     misc_feature    complement(1404..1456)
                     /label=E1b
     misc_feature    1472..1488
                     /label=UAS
     misc_feature    1491..1507
                     /label=UAS
     misc_feature    1510..1526
                     /label=UAS
     misc_feature    1529..1545
                     /label=UAS
     misc_feature    1548..1564
                     /label=UAS
     misc_feature    1577..1672
                     /label=Spacer
     misc_feature    1698..1714
                     /label=UAS
     misc_feature    1717..1733
                     /label=UAS
     misc_feature    1736..1752
                     /label=UAS
     misc_feature    1755..1771
                     /label=UAS
     misc_feature    1774..1790
                     /label=UAS
     misc_feature    1806..1858
                     /label=E1b
     CDS             1875..1919
                     /codon_start=1
                     /product="Fyn kinase membrane targeting signal"
                     /label=Fyn
                     /translation="MGCVQCKDKEATKLT"
     CDS             1926..2621
                     /codon_start=1
                     /label=zmScarlet-I
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     CDS             2637..2690
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2691..3071
                     /codon_start=1
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAKD"
     CDS             3087..3806
                     /codon_start=1
                     /label=zmTurquoise2
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(3823..3840)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3847..4268
                     /label=AFPpA
                     /note="3'UTR containing PolyA signal of the ocean pout 
                     antifreeze protein gene   "
     polyA_signal    4347..4481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4486..4503
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(4527..5456)
                     /codon_start=1
                     /label=emiRFP670(Zebrafish)
                     /translation="MAEGSVARQPDLLTCEHEEIHLAGSIQPHGALLVVSEHDHRVIQA
                     SANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLM
                     HRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVM
                     VYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVP
                     LEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHY
                     LPRFIRFELRAICKRLAERIATRITALES"
     CDS             complement(5478..6320)
                     /codon_start=1
                     /label=EB3
                     /translation="MAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYC
                     QFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDN
                     FEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSP
                     TGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHETDAQILELNQQLVDLKLTVDGLEKE
                     RDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY
                     "
     misc_feature    complement(6352..6404)
                     /label=E1b
     misc_feature    6420..6436
                     /label=UAS
     misc_feature    6439..6455
                     /label=UAS
     misc_feature    6458..6474
                     /label=UAS
     misc_feature    6477..6493
                     /label=UAS
     misc_feature    6496..6512
                     /label=UAS
     misc_feature    6541..6636
                     /label=Spacer
     misc_feature    6662..6678
                     /label=UAS
     misc_feature    6681..6697
                     /label=UAS
     misc_feature    6700..6716
                     /label=UAS
     misc_feature    6719..6735
                     /label=UAS
     misc_feature    6738..6754
                     /label=UAS
     misc_feature    6770..6822
                     /label=E1b
     CDS             6833..6883
                     /codon_start=1
                     /label=Leifeact
                     /translation="MGVADLIKKFESISKEE"
     CDS             6908..7627
                     /codon_start=1
                     /label=mClover3
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     polyA_signal    7658..7792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(7895..7913)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    7920..8120
                     /label=Tol2miniR
     primer_bind     complement(8149..8165)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8173..8189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8197..8227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(8551..9139)
                     /direction=LEFT
                     /label=ori
                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication;Geneious name: ori rep origin"
     CDS             complement(9310..10170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;Geneious name: bla 
                     CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10171..10275)
                     /gene="bla"
                     /label=bla AmpR promoter
                     /note="AmpR promoter;Geneious name: bla promoter"
ORIGIN
        1 ctaaattgta agcgttaatc ccatatgggc agaggtgtaa aaagtactca aaaattttac
       61 tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg
      121 ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta
      181 gatctgtaat acgactcact atagggacat agaatgcagg tacgtaccgg gcccaatgca
      241 ttggcgccgc ggccggccgc gaattaaaaa acctcccaca cctccccctg aacctgaaac
      301 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
      361 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      421 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta
      481 tagttctagc ctcgagacta gttttacatg gcgtagagcc ctaccgccac agtagcgacg
      541 aggaaactaa gagtcagaac ccatctcagg agcggagcct gactacgagt gttcaaagga
      601 ggctttccgc gcggcgtttc gacaggtgcg ctgtcctgca ccttattaga agccctctgg
      661 cgcaagcccg gagccctaga aggggactga tccttcgtat cgtgcgtcaa cagttcttgg
      721 agctcctcga acaactggat gttgaggaga aaggccgttt ttgcttcttc tataacacgt
      781 tgccgaactg ccggcgtcat ctcgagactg ttcatgcggc ttcggtagag ctgtttaaat
      841 tttgtagcac ttgcaatgtt tggaaacgta aaaaaagcca gcccctctcc actggagggc
      901 agatcgagtg ccttctgcgc gatctttttg agaacttgcc cacctgacag atcgccgagg
      961 tatcgtgtat aggcgtgggc gaccagcaat tccggttcag tgcggccaac ctcatgcaat
     1021 cttttcacgt aacgctgcat tgctggtgta tagggaatca cctcctgcca tctgggcccg
     1081 taccaaaaag ccaaatcttg ttcgagcgcc gctttgcggt gaagctcctc tggaaaatac
     1141 acgggggcaa atacgggact ttccttattc ctctctattt cttcttccag ggcaacgtag
     1201 atgtggtaaa ggctcgccat gaccaacttg aagccgtcac gcgtcacttg tcccttctgg
     1261 aaatttctca taaattctgc attctcggct tgggtatgga cttctttagt cgcttccttc
     1321 aaagcctctg acaaatcctg gggcatggag tcaggttgtg gtcgttccat gtttgcgata
     1381 tcgtttaaac agatcgttta aacgtgtgga ggagctcaaa gtgaggctga gacgcgatgg
     1441 gatccattat ataccctcta gagtctccgc tcggaggaca gtactccgct cggaggacag
     1501 tactccgctc ggaggacagt actccgctcg gaggacagta ctccgctcgg aggacagtac
     1561 tccgacctgc agcccgtcct agctagggct gctctccaat ctctgtcatg gcagggccac
     1621 tggcatccct gaagttggtg gagaccacct cactccactc agcatacagc tcatccagtc
     1681 ctcttagggc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     1741 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact
     1801 ctagagggta tataatggat cccatcgcgt ctcagcctca ctttgagctc ctccacacga
     1861 attcgatatc caccatgggc tgtgtgcaat gtaaggataa agaagcaaca aaactgacgt
     1921 cgacgatggt gagcaagggc gaggcagtga tcaaggagtt catgagattc aaggtgcaca
     1981 tggagggaag catgaacggc cacgagttcg agatcgaggg agagggagag ggaagacctt
     2041 acgagggaac acagacagct aagctgaagg tgacaaaggg aggaccactg cctttctctt
     2101 gggacatcct gagccctcag ttcatgtacg gaagcagagc tttcatcaag caccctgctg
     2161 acatccctga ctactacaag cagagcttcc ctgagggatt caagtgggag agagtgatga
     2221 acttcgagga cggaggagct gtgacagtga cacaggacac aagcctggag gacggaacac
     2281 tgatctacaa ggtgaagctg agaggaacaa acttccctcc tgacggacct gtgatgcaga
     2341 agaagacaat gggatgggag gcttctacag agagactgta ccctgaggac ggagtgctga
     2401 agggagacat caagatggct ctgagactga aggacggagg aagatacctg gctgacttca
     2461 agacaacata caaggctaag aagcctgtgc agatgcctgg agcttacaac gtggacagaa
     2521 agctggacat cacaagccac aacgaggact acacagtggt ggagcagtac gagagaagcg
     2581 agggaagaca cagcaccggc ggcatggacg agctgtacaa gactagtgga tccggcgagg
     2641 gaagaggaag tctgctgacc tgcggagacg tggaggagaa ccctggacct atgccagagc
     2701 cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga
     2761 agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtaca
     2821 aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggctatg ggcatcatga
     2881 actcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt
     2941 acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc
     3001 ctggggagtt ggccaagcat gccgtgtccg agggtactaa ggccatcacc aagtacacca
     3061 gcgctaagga cccaccggtc gccaccatgg tgtcaaaagg agaggaactg tttactggag
     3121 tcgtgcctat cctggtggag ctggatggag atgtgaatgg acacaagttc tctgtgtctg
     3181 gagagggaga gggagatgct acatatggaa agctgacact gaagttcatc tgtacaacag
     3241 gaaagctgcc tgtgccttgg cctacactgg tgacaacact gtcttgggga gtgcagtgtt
     3301 ttgctagata ccctgaccac atgaagcagc atgacttctt caagtctgct atgcctgagg
     3361 gatatgtgca ggagagaaca atcttcttca aggatgatgg aaactacaag acaagagctg
     3421 aggtgaagtt tgagggagac acactggtga acagaattga gctgaaggga attgacttca
     3481 aggaggatgg aaacatcctg ggacacaagc tggagtacaa ttatttctct gacaatgtct
     3541 acatcacagc tgacaagcag aagaatggaa tcaaggctaa cttcaagatc agacacaaca
     3601 ttgaggatgg aggagtgcag ctggctgacc actaccagca gaacacacct attggagatg
     3661 gacctgtgct gctgcctgac aaccactacc tgagcacaca gtctaagctg agcaaggacc
     3721 ctaatgagaa gagagaccac atggtgctgc tggagtttgt gacagctgct ggaatcacac
     3781 tgggcatgga cgagctgtac aagtaaactc gagcctctag aactatagtg agtcgtatta
     3841 cctagtaatt aagtctcagc caccgttaac tgaacatgtc aaaacctgtg gagactgttg
     3901 agatttgatg ttctgaaaag ataaagccta taaataaaat gttgcccaaa tttcctgcct
     3961 gatgtttttc tttgtctttg ctacatggct ttgctgctcg gatcggctca ctctgtgtat
     4021 gccacgttca ctttgtactc tccttctcac ggtaggttta ttatttttag atgtgcagtt
     4081 agtttctgtg aaataacaca ccacacactg atattgtctg tgcattgact tggtgagtgc
     4141 acattgtttt tgatcttgac atatttatat ttgattgatc aggtgaactg tgtgaatcta
     4201 aagtgctcca tacagatgtt ctgcattgaa aatattctca ttttattagt ggaagtgagt
     4261 gtatgccagc gcaggtaccc gaattaaaaa acctcccaca cctccccctg aacctgaaac
     4321 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     4381 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4441 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta
     4501 tagttctagc ctcgagacta gatttagctc tccagagctg tgattcttgt agcgattctc
     4561 tcagccagtc tcttgcagat agctctcagc tcgaatctga tgaatctagg caggtagtgg
     4621 tggcacacca ccagtcccca cagctttcct cccaccacca ggctcacagc cagtgtagct
     4681 ctcactccca tgtccttcag gaactgcagg tggcaagggc tcatgcttct caggaagcat
     4741 ccgctcatgt ccaggtctct tcctgtcaga gggctcagtc taggctccag aggcacaggc
     4801 tggtatgtca cgtccaccag cactctcact ctctgtctca cgtacagctg tctagccatc
     4861 tgaggcactg tgctgctagg gtatctgttt ccgaagtagc tctccagtcc aggcacgtgg
     4921 cactcgctga acaccagtcc gtgtccctgc tcgtcgaatc tatacaccat cactctgtcg
     4981 tatcctgtgc actgctggaa cagcagcact gtgtcgtcgc acagagctct caggcttcca
     5041 gctgttctga ttctctccag agcaggagcc agtgttccgc tcaggtcgat gctaggtcca
     5101 gctctctcca gctcgatgat cagtcctccc tcaggaggtc tgtgcatcag tccgcagtac
     5161 tctgtgctag ggtttccgat tctgcatctc acagccacag gcattccctc agctgtaggg
     5221 tccaggtgag gcaggatctt gatcagcagg tctccgtcga tctcagccag aggcactccc
     5281 agcacgcttc ccaggttcag gaactcagca gcgttagcag aagcctggat cactctgtgg
     5341 tcgtgctcgc tcaccaccag cagagctccg tgaggctgga tgcttccagc caggtggatc
     5401 tcctcgtgct cgcatgtcag caggtcaggc tgtctagcca cgcttccctc agccatggtg
     5461 gcgaccggtg gatccaggta ctcgtcctgg tcttcttgtt gatgctcttc aatctcatcg
     5521 tcctcagggg gtgcgaatcc ttcctctgtg gcatagagga tgccaatgat gcctgagata
     5581 acagggctgt tttcactttc atgctcctgg cagatgagct cgatgtcacg aagtttgctg
     5641 aagtagaagt cacgttcctt ctccagccca tccactgtca gcttcaagtc caccagctgt
     5701 tggttgagtt caagaatttg ggcatcagtc tcatggccgc catttcgggc tgatggagga
     5761 ttcttccgga gaatgcaggg gggggccaca ttgctcagcc ggccagaggt ctgcatgttt
     5821 tttgggcctg tgggggacgt cctctgtgga actgctgtgc caatgagttt cttggatttg
     5881 ttgaagatct gatcacctgg gttaggaggt ggcgctacgt cctggccctg ccgcgccagc
     5941 agagggttgt aatcctttcc atcatagttt gcgtcaaaga atttcttaaa ccactgaata
     6001 aactcaaaat tatcttggaa ttttcctttc actaatttct ctacaggaat gattttgtca
     6061 acacccatct tcttgaaagc tgcttgcagc accttgaagt tgtggatgta ttcatgctct
     6121 agtttggcct ggaacttcac tttcctcaag tgcacacagc cggggaagag catgtccatg
     6181 aactggcagt aggctgcccc tgaacaaagc tgttctatct tggtatagtt gaggtgcagg
     6241 gagtcgttga cccatgcaag catatcatgg cgactcagat tttcactggt cacagatgtg
     6301 gagtacacat tgacggccat ggtggatatc gtttaaacag atcgtttaaa cgtgtggagg
     6361 agctcaaagt gaggctgaga cgcgatggga tccattatat accctctaga gtctccgctc
     6421 ggaggacagt actccgctcg gaggacagta ctccgctcgg aggacagtac tccgctcgga
     6481 ggacagtact ccgctcggag gacagtactc cgacctgcag cccgggggat cgtcgacagg
     6541 tcctagctag ggctgctctc caatctctgt catggcaggg ccactggcat ccctgaagtt
     6601 ggtggagacc acctcactcc actcagcata cagctcatcc agtcctctta gggctgcagg
     6661 tcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     6721 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactctagag ggtatataat
     6781 ggatcccatc gcgtctcagc ctcactttga gctcctccac acgaattcca ccatgggagt
     6841 ggctgacctg atcaagaaat tcgagagcat ctctaaggag gaaggagacc ctccagtggc
     6901 gtcgacgatg gtgagcaagg gcgaggagct gttcacagga gtggtgccta tcctggtgga
     6961 gctggacgga gacgtgaacg gacacaagtt cagcgtgaga ggagagggag agggagacgc
     7021 tacaaacgga aagctgacac tgaagttcat ctgcacaaca ggaaagctgc ctgtgccttg
     7081 gcctacactg gtgacaacat tcggatacgg agtggcttgt ttcagcagat accctgacca
     7141 catgaagcag cacgacttct tcaagtctgc tatgcctgag ggatacgtgc aggagagaac
     7201 aatcagcttc aaggacgacg gaacatacaa gacaagagct gaggtgaagt tcgagggaga
     7261 cacactggtg aacagaatcg agctgaaggg aatcgacttc aaggaggacg gaaacatcct
     7321 gggacacaag ctggagtaca acttcaacag ccactacgtg tatatcacag ctgacaagca
     7381 gaagaactgt atcaaggcta acttcaagat cagacacaac gtggaggacg gaagcgtgca
     7441 gctggctgac cactaccagc agaacacacc tatcggagac ggacctgtgc tgctgcctga
     7501 caaccactac ctgagccacc agagcaagct gtctaaagac cctaacgaga agagagacca
     7561 catggtgctg ctggagttcg tgacagctgc tggaatcaca cacggcatgg acgagctgta
     7621 caagtaaatc tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat
     7681 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     7741 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     7801 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc
     7861 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgggccca
     7921 agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg agtaaaaagt
     7981 actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt ttaattgtac
     8041 tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag taaaattact
     8101 caagtacttt acacctctgg gtactgtttc gcgcttggcg taatcatggt catagctgtt
     8161 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8221 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8281 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8341 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8401 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8461 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8521 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8581 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8641 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8701 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8761 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8821 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8881 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8941 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9001 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9061 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9121 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9181 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9241 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9301 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9361 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9421 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9481 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9541 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9601 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9661 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9721 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9781 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9841 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9901 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9961 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10021 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10081 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10141 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10201 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10261 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
