LOCUS       Exported               10529 bp ds-DNA     circular SYN 15-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Kazuhiko
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 20, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..19)
                     /direction=LEFT
                     /label=Deleated f1 ori
                     /note="f1 ori;f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis;Geneious name: 
                     f1 ori rep origin"
     misc_feature    30..180
                     /label=Tolmini-L
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    319..453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        458..475
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(496..1362)
                     /codon_start=1
                     /product="zebrafish codon optimized human HO1"
                     /label=zHO1
                     /translation="MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRD
                     GFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEV
                     IPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEG
                     LAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLT
                     HDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATV
                     AVGLYAM"
     misc_feature    complement(1396..1448)
                     /label=E1b
     misc_feature    1464..1480
                     /label=UAS
     misc_feature    1483..1499
                     /label=UAS
     misc_feature    1502..1518
                     /label=UAS
     misc_feature    1521..1537
                     /label=UAS
     misc_feature    1540..1556
                     /label=UAS
     misc_feature    1569..1664
                     /label=Spacer
     misc_feature    1690..1706
                     /label=UAS
     misc_feature    1709..1725
                     /label=UAS
     misc_feature    1728..1744
                     /label=UAS
     misc_feature    1747..1763
                     /label=UAS
     misc_feature    1766..1782
                     /label=UAS
     misc_feature    1798..1850
                     /label=E1b
     CDS             1864..2583
                     /codon_start=1
                     /label=zmTurquoise2
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(2592..2609)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2616..3037
                     /label=AFPpA
                     /note="3'UTR containing PolyA signal of the ocean pout 
                     antifreeze protein gene   "
     polyA_signal    3116..3250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3255..3272
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3293..3661)
                     /codon_start=1
                     /label=zf LC3B
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPNKIPVIIERYKGEKQLP
                     ILDKTKFLVPDHVNMSELIKIIRRRLQLNSNQAFFLLVNGHSMVSVSTAIFEVYERERD
                     EDGFLYMVYASQETFGFQ"
     CDS             complement(3710..4426)
                     /codon_start=1
                     /product="zebrafish codon optimized mClover3"
                     /label=zmClover3
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             complement(4428..4444)
                     /codon_start=1
                     /label=zLinker
                     /translation="GGSGG"
     CDS             complement(4445..5176)
                     /codon_start=1
                     /label=zTagRFP-T
                     /note="zebrafish codon optimized TagRFP-T"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     misc_feature    complement(5209..5261)
                     /label=E1b
     misc_feature    5277..5293
                     /label=UAS
     misc_feature    5296..5312
                     /label=UAS
     misc_feature    5315..5331
                     /label=UAS
     misc_feature    5334..5350
                     /label=UAS
     misc_feature    5353..5369
                     /label=UAS
     misc_feature    5398..5493
                     /label=Spacer no CpG
     misc_feature    5519..5535
                     /label=UAS
     misc_feature    5538..5554
                     /label=UAS
     misc_feature    5557..5573
                     /label=UAS
     misc_feature    5576..5592
                     /label=UAS
     misc_feature    5595..5611
                     /label=UAS
     misc_feature    5627..5679
                     /label=E1b
     misc_feature    5686..5690
                     /label=unclear sequence
     CDS             5696..6916
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=RAT LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             6926..7855
                     /codon_start=1
                     /label=emiRFP670(Zebrafish)
                     /translation="MAEGSVARQPDLLTCEHEEIHLAGSIQPHGALLVVSEHDHRVIQA
                     SANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLM
                     HRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVM
                     VYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVP
                     LEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHY
                     LPRFIRFELRAICKRLAERIATRITALES"
     polyA_signal    7889..8023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(8126..8144)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    8151..8351
                     /label=Tol2miniR
     primer_bind     complement(8380..8396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8404..8420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8428..8458)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(8782..9370)
                     /direction=LEFT
                     /label=ori
                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication;Geneious name: ori rep origin"
     CDS             complement(9541..10401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;Geneious name: bla 
                     CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10402..10506)
                     /gene="bla"
                     /label=bla AmpR promoter
                     /note="AmpR promoter;Geneious name: bla promoter"
ORIGIN
        1 ctaaattgta agcgttaatc ccatatgggc agaggtgtaa aaagtactca aaaattttac
       61 tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg
      121 ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta
      181 gatctgtaat acgactcact atagggacat agaatgcagc gggcccaatg cattggcgcc
      241 gcggccggcc gcgaattaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
      301 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      361 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      421 aaactcatca atgtatctta tcatgtctgg atctacgtaa tacgactcac tatagttcta
      481 gcctcgagac tagttttaca tggcgtagag ccctaccgcc acagtagcga cgaggaaact
      541 aagagtcaga acccatctca ggagcggagc ctgactacga gtgttcaaag gaggctttcc
      601 gcgcggcgtt tcgacaggtg cgctgtcctg caccttatta gaagccctct ggcgcaagcc
      661 cggagcccta gaaggggact gatccttcgt atcgtgcgtc aacagttctt ggagctcctc
      721 gaacaactgg atgttgagga gaaaggccgt ttttgcttct tctataacac gttgccgaac
      781 tgccggcgtc atctcgagac tgttcatgcg gcttcggtag agctgtttaa attttgtagc
      841 acttgcaatg tttggaaacg taaaaaaagc cagcccctct ccactggagg gcagatcgag
      901 tgccttctgc gcgatctttt tgagaacttg cccacctgac agatcgccga ggtatcgtgt
      961 ataggcgtgg gcgaccagca attccggttc agtgcggcca acctcatgca atcttttcac
     1021 gtaacgctgc attgctggtg tatagggaat cacctcctgc catctgggcc cgtaccaaaa
     1081 agccaaatct tgttcgagcg ccgctttgcg gtgaagctcc tctggaaaat acacgggggc
     1141 aaatacggga ctttccttat tcctctctat ttcttcttcc agggcaacgt agatgtggta
     1201 aaggctcgcc atgaccaact tgaagccgtc acgcgtcact tgtcccttct ggaaatttct
     1261 cataaattct gcattctcgg cttgggtatg gacttcttta gtcgcttcct tcaaagcctc
     1321 tgacaaatcc tggggcatgg agtcaggttg tggtcgttcc atgtttgcga tatcgtttaa
     1381 acagatcgtt taaacgtgtg gaggagctca aagtgaggct gagacgcgat gggatccatt
     1441 atataccctc tagagtctcc gctcggagga cagtactccg ctcggaggac agtactccgc
     1501 tcggaggaca gtactccgct cggaggacag tactccgctc ggaggacagt actccgacct
     1561 gcagcccgtc ctagctaggg ctgctctcca atctctgtca tggcagggcc actggcatcc
     1621 ctgaagttgg tggagaccac ctcactccac tcagcataca gctcatccag tcctcttagg
     1681 gctgcaggtc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     1741 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggaga ctctagaggg
     1801 tatataatgg atcccatcgc gtctcagcct cactttgagc tcctccacac gaattctgca
     1861 aacatggtgt caaaaggaga ggaactgttt actggagtcg tgcctatcct ggtggagctg
     1921 gatggagatg tgaatggaca caagttctct gtgtctggag agggagaggg agatgctaca
     1981 tatggaaagc tgacactgaa gttcatctgc acaacaggaa agctgcctgt gccttggcct
     2041 acactggtga caacactgtc ttggggagtg cagtgttttg ctagataccc tgaccacatg
     2101 aagcagcatg acttcttcaa gtctgctatg cctgagggat atgtgcagga gagaacaatc
     2161 ttcttcaagg atgatggaaa ctacaagaca agagctgagg tgaagtttga gggagacaca
     2221 ctggtgaaca gaattgagct gaagggaatt gacttcaagg aggatggaaa catcctggga
     2281 cacaagctgg agtacaatta tttctctgac aatgtctaca tcacagctga caagcagaag
     2341 aatggaatca aggctaactt caagatcaga cacaacattg aggatggagg agtgcagctg
     2401 gctgaccact accagcagaa cacacctatt ggagatggac ctgtgctgct gcctgacaac
     2461 cactacctga gcacacagtc taagctgagc aaggacccta atgagaagag agaccacatg
     2521 gtgctgctgg agtttgtgac agctgctgga atcacactgg gcatggacga gctgtacaag
     2581 taaatctaga actatagtga gtcgtattac ctagtaatta agtctcagcc accgttaact
     2641 gaacatgtca aaacctgtgg agactgttga gatttgatgt tctgaaaaga taaagcctat
     2701 aaataaaatg ttgcccaaat ttcctgcctg atgtttttct ttgtctttgc tacatggctt
     2761 tgctgctcgg atcggctcac tctgtgtatg ccacgttcac tttgtactct ccttctcacg
     2821 gtaggtttat tatttttaga tgtgcagtta gtttctgtga aataacacac cacacactga
     2881 tattgtctgt gcattgactt ggtgagtgca cattgttttt gatcttgaca tatttatatt
     2941 tgattgatca ggtgaactgt gtgaatctaa agtgctccat acagatgttc tgcattgaaa
     3001 atattctcat tttattagtg gaagtgagtg tatgccagcg caggtacccg aattaaaaaa
     3061 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3241 tgtctggatc tacgtaatac gactcactat agttctagcc tcgagactag atttactgaa
     3301 atccaaatgt ctcctgggaa gcgtagacca tatacagaaa gccgtcttcg tctctttccc
     3361 gttcatagac ctcaaaaatg gcagtggaca cagataccat gctgtgaccg ttgaccagca
     3421 ggaagaaagc ctggttggag ttgagttgga ggcgtcgcct gataatctta ataagttcgc
     3481 tcatgttaac gtggtcagga actagaaact tagttttgtc aagaatcggc agttgcttct
     3541 ctcccttgta tctctcgatg atgaccggga tcttgtttgg atgctgttcc cggatcagcc
     3601 gtacatcctc cacccgctgc tcaaatgtcc tcctttgttt aaatgtcttt tccgaaggca
     3661 tcgtcgaccc cccagagcca cctccgcctg atccgccacc tccgctagac ttgtacagct
     3721 cgtccatgcc gtgtgtgatt ccagcagctg tcacgaactc cagcagcacc atgtggtctc
     3781 tcttctcgtt agggtcttta gacagcttgc tctggtggct caggtagtgg ttgtcaggca
     3841 gcagcacagg tccgtctccg ataggtgtgt tctgctggta gtggtcagcc agctgcacgc
     3901 ttccgtcctc cacgttgtgt ctgatcttga agttagcctt gatacagttc ttctgcttgt
     3961 cagctgtgat atacacgtag tggctgttga agttgtactc cagcttgtgt cccaggatgt
     4021 ttccgtcctc cttgaagtcg attcccttca gctcgattct gttcaccagt gtgtctccct
     4081 cgaacttcac ctcagctctt gtcttgtatg ttccgtcgtc cttgaagctg attgttctct
     4141 cctgcacgta tccctcaggc atagcagact tgaagaagtc gtgctgcttc atgtggtcag
     4201 ggtatctgct gaaacaagcc actccgtatc cgaatgttgt caccagtgta ggccaaggca
     4261 caggcagctt tcctgttgtg cagatgaact tcagtgtcag ctttccgttt gtagcgtctc
     4321 cctctccctc tcctctcacg ctgaacttgt gtccgttcac gtctccgtcc agctccacca
     4381 ggataggcac cactcctgtg aacagctcct cgcccttgct caccattgtt cctccgcttc
     4441 ctcccttata cagctcgtcc atgccattca gcttgtgtcc cagcttgcta ggcaggtcac
     4501 agtatctagc cacagccacc tcatgctgct ccacatatgt ctccttgtca gcctccttga
     4561 ttctctccag tctgtggtcc acatagtaca ctccaggcat cttcaggttc ttagcaggct
     4621 tcttgcttct gtatgttgtc ttgaagttac agatcaggtg tcctcctccc accagcttca
     4681 gagccatgtc tgttcttccc tccagtcctc catcagcagg gtacagcatc tctgtgttag
     4741 cctcccatcc cagtgtcttc ttctgcatca caggtccatt gctagggaag ttcactcctc
     4801 tgatcttcac attgtagatc agacatccat cctggaggct tgtgtcctgt gtagctgtca
     4861 gcactcctcc atcctcatat gttgtcactc tctcccatgt gaatccctca gggaagctct
     4921 gcttgaagaa gtcagggatt ccctgtgtgt ggttgatgaa tgttctgctt ccatacatga
     4981 agctggtagc caggatgtca aaagcaaaag gcagaggtcc tccctccacc accttgattc
     5041 tcattgtctg tgttccctca taaggctttc cctctccctc agatgtgcac ttgaagtggt
     5101 ggttgttcac tgttccctcc atatacagct tcatgtgcat gttctccttg atcagctcct
     5161 ctcccttgct caccatggtg ggatatcgtt taaacagatc gtttaaacgt gtggaggagc
     5221 tcaaagtgag gctgagacgc gatgggatcc attatatacc ctctagagtc tccgctcgga
     5281 ggacagtact ccgctcggag gacagtactc cgctcggagg acagtactcc gctcggagga
     5341 cagtactccg ctcggaggac agtactccga cctgcagccc gggggatcgt cgacaggtcc
     5401 tagctagggc tgctctccaa tctctgtcat ggcagggcca ctggcatccc tgaagttggt
     5461 ggagaccacc tcactccact cagcatacag ctcatccagt cctcttaggg ctgcaggtcg
     5521 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     5581 tactgtcctc cgagcggagt actgtcctcc gagcggagac tctagagggt atataatgga
     5641 tcccatcgcg tctcagcctc actttgagct cctccacacg aattcgatat ccaccatggc
     5701 ggccccgggc gcccgtcggc cgctgctcct gttgctgctg gcaggccttg cacacagcgc
     5761 cccagcactg ttcgaggtga aagacaacaa cggcacagcg tgtataatgg ccagcttctc
     5821 tgcctccttt ctgaccacct atgaggctgg acatgtttct aaggtctcga atatgaccct
     5881 gccagcctct gcagaagtcc tgaagaatag cagctcttgt ggtgaaaaga atgcttctga
     5941 gcccaccctc gcaatcacct ttggagaagg atatttactg aaactcacct tcacaaaaaa
     6001 cacaacacgt tacagtgtcc agcacatgta tttcacatat aacctgtcag acacacaatt
     6061 ctttcccaat gccagctcca aagggcccga cactgtggat tccacaactg acatcaaggc
     6121 agacatcaac aaaacatacc gatgtgtcag cgacatcagg gtctacatga agaatgtgac
     6181 cattgtgctc tgggacgcta ctatccaggc ctacctgccg agtagcaact tcagcaagga
     6241 agagacacgc tgcccacagg atcaaccttc cccaactact gggccaccca gcccctcacc
     6301 accacttgtg cccacaaacc ccagtgtgtc caagtacaat gtgactggtg acaatggaac
     6361 ctgcctgctg gcctctatgg cactgcaact caacatcacc tacatgaaga aggacaacac
     6421 gactgtgacc agagcattca acatcaaccc aagtgacaaa tatagtggga cttgcggtgc
     6481 ccagttggtg accctgaagg tggggaacaa gagcagagtc ctggagctgc agtttgggat
     6541 gaatgccact tctagcctgt ttttcctgca aggagttcag ttgaacatga ctcttcctga
     6601 tgccatagag cccacgttca gcacctccaa ctattccctg aaagctcttc aggccagtgt
     6661 cggcaactca tacaagtgca acagtgagga gcacatcttt gtcagcaagg cgctcgccct
     6721 caatgtcttc agcgtgcaag tccaggcttt cagggtagaa agtgacaggt ttgggtctgt
     6781 ggaagagtgt gtacaggacg gtaacaacat gctgatcccc attgctgtgg gcggggccct
     6841 ggcagggctg gtcctcatcg tcctcatcgc ctacctcatc ggcaggaaga ggagtcacgc
     6901 gggctatcag accatcgggt cgacgatggc tgagggaagc gtggctagac agcctgacct
     6961 gctgacatgc gagcacgagg agatccacct ggctggaagc atccagcctc acggagctct
     7021 gctggtggtg agcgagcacg accacagagt gatccaggct tctgctaacg ctgctgagtt
     7081 cctgaacctg ggaagcgtgc tgggagtgcc tctggctgag atcgacggag acctgctgat
     7141 caagatcctg cctcacctgg accctacagc tgagggaatg cctgtggctg tgagatgcag
     7201 aatcggaaac cctagcacag agtactgcgg actgatgcac agacctcctg agggaggact
     7261 gatcatcgag ctggagagag ctggacctag catcgacctg agcggaacac tggctcctgc
     7321 tctggagaga atcagaacag ctggaagcct gagagctctg tgcgacgaca cagtgctgct
     7381 gttccagcag tgcacaggat acgacagagt gatggtgtat agattcgacg agcagggaca
     7441 cggactggtg ttcagcgagt gccacgtgcc tggactggag agctacttcg gaaacagata
     7501 ccctagcagc acagtgcctc agatggctag acagctgtac gtgagacaga gagtgagagt
     7561 gctggtggac gtgacatacc agcctgtgcc tctggagcct agactgagcc ctctgacagg
     7621 aagagacctg gacatgagcg gatgcttcct gagaagcatg agcccttgcc acctgcagtt
     7681 cctgaaggac atgggagtga gagctacact ggctgtgagc ctggtggtgg gaggaaagct
     7741 gtggggactg gtggtgtgcc accactacct gcctagattc atcagattcg agctgagagc
     7801 tatctgcaag agactggctg agagaatcgc tacaagaatc acagctctgg agagctaaat
     7861 ctagaactat agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt
     7921 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     7981 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     8041 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccgccac
     8101 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgggccc aagtgatctc
     8161 caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt
     8221 tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa
     8281 gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt
     8341 tacacctctg ggtactgttt cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8401 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8461 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8521 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8581 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8641 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8701 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8761 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8821 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8881 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8941 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9001 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9061 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9121 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9181 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     9241 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9301 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9361 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9421 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9481 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9541 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9601 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9661 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9721 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9781 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9841 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9901 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9961 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10021 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10081 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10141 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10201 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10261 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10321 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10381 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10441 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10501 tccgcgcaca tttccccgaa aagtgccac
//
