LOCUS       pEPYCeGM0011            4105 bp ds-DNA     circular     14-DEC-2022
DEFINITION  .
COMMENT     pENTR_AtDREB26_HiBit GateWay(tm) BP reaction between
            attB_DREB26_HiBit.gb.xdna as an Expression fragment and
            pdonr207.gb.xdna as a Donor vector.
FEATURES             Location/Qualifiers
     terminator      75..102
                     /label="rrnB\T2\transcription\terminator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     terminator      234..277
                     /label="rrnB\T1\transcription\terminator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          288..305
                     /label="SeqL-A"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     primer          302..326
                     /label="pDONRvÖE07\forward\primer"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     misc_feature    435..1124
                     /label="AtDREB26 CDS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     tag             1131..1163
                     /label="HiBit"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_recomb     complement(1171..1191)
                     /label="attB2"
                     /ApEinfo_revcolor="#2f4f4f"
                     /ApEinfo_fwdcolor="#2f4f4f"
     misc_feature    complement(1171..1191)
                     /label="Gateway Recomb2"
                     /ApEinfo_revcolor="#dc143c"
                     /ApEinfo_fwdcolor="#dc143c"
     primer          complement(1291..1310)
                     /label="SeqL-B"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     primer          complement(1291..1314)
                     /label="pDONRvÖE07\reverse\primer"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     CDS             complement(2050..2583)
                     /label="Gm(R)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(2772..2799)
                     /label="Pc\promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag ccaggaagag
       61 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
      121 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
      181 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      241 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
      301 ctcgcgttaa cgctagcatg gatctcgggc cccaaataat gattttattt tgactgatag
      361 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca aCtttGTACA
      421 AAAAAGCAGG CTTCATGGTG AAACAAGAAC GCAAGATCCA AACCAGCAGC ACAAAAAAGG
      481 AAATGCCTTT GTCATCATCA CCATCTTCTT CTTCTTCTTC ATCTTCTTCC TCGTCTTCGT
      541 CTTCGTGTAA GAACAAGAAC AAGAAGAGTA AGATTAAGAA GTACAAAGGA GTGAGGATGA
      601 GAAGTTGGGG ATCATGGGtt tcTGAGATTA GGGCACCAAA TCAAAAGACA AGGATTTGGT
      661 TAGGTTCTTA CTCAACAGCT GAAGCAGCTG CTAGAGCTTA CGATGTTGCA CTCTTATGTC
      721 TCAAAGGCCC TCAAGCCAAT CTCAACTTCC CTACTTCTTC TTCTTCTCAT CATCTTCTTG
      781 ATAATCTCTT AGATGAAAAT ACCCTTTTGT CCCCCAAATC CATCCAAAGA GTAGCTGCTC
      841 AAGCTGCCAA CTCATTTAAC CATTTTGCCC CTACTTCATC AGCCGTCTCG TCACCGTCCG
      901 ATCATGATCA TCACCATGAT GATGGGATGC AATCTTTGAT GGGATCTTTT GTGGACAATC
      961 ATGTGTCTTT GATGGATTCA ACATCTTCAT GGTATGATGA TCATAATGGG ATGTTCTTGT
     1021 TTGATAATGG AGCTCCATTC AATTACTCTC CTCAACTAAA CTCGACGACG ATGCTCGATG
     1081 AATACTTCTA CGAAGATGCT GACATTCCGC TTTGGAGTTT CAATggttcg GTGAGCGGCT
     1141 GGCGGCTGTT CAAGAAGATT AGCTGAAACC CAGCTTTCTT GTACaaagtg ggcattataa
     1201 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1261 ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1321 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1381 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1441 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1501 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1561 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     1621 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1681 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     1741 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     1801 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     1861 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1921 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     1981 tcacttgata accttatttt tgacgagggg aaattaggcc gggaagccga tctcggcttg
     2041 aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat
     2101 gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat
     2161 cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat
     2221 gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg
     2281 ctgctcaaac ctgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa
     2341 ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg
     2401 ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc
     2461 ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga
     2521 gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa
     2581 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     2641 atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg
     2701 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
     2761 acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc
     2821 gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt
     2881 cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct
     2941 tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag gagatcggaa
     3001 gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc
     3061 tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat agttctagtg
     3121 gttggctact aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     3181 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     3241 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     3301 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     3361 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3421 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3481 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3541 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3601 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3661 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3721 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3781 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3841 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3901 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3961 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4021 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4081 cttttgctgg ccttttgctc acatg
//