LOCUS       pEPYCdKN0010            8257 bp ds-DNA     circular     09-DEC-2022
DEFINITION  .
COMMENT     pH9GW_9xHis_AtNLP7_HiBit GateWay(tm) LR reaction between
            pENTR_AtNLP7_HiBit.xdna as an Entry vector and ph9gw.gb.xdna as a
            Destination vector.
FEATURES             Location/Qualifiers
     promoter        23..39
                     /label="T7\Promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          23..42
                     /label="T7\Primer"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     misc_feature    96..102
                     /label="RBS"
                     /ApEinfo_revcolor="#dc143c"
                     /ApEinfo_fwdcolor="#dc143c"
     CDS             110..3103
                     /label="Translation 110-3103"
                     /translation="MKHHHHHHHHHGGLESTSLYKKAGFMCEPDDNSARNGVTTQPSRSRELLMDVDDLDLDGSWPLDQIPYLSSSNRMISPIFVSSSSEQPCSPLWAFSDGGGNGFHHATSGGDDEKISSVSGVPSFRLAEYPLFLPYSSPSAAENTTEKHNSFQFPSPLMSLVPPENTDNYCVIKERMTQALRYFKESTEQHVLAQVWAPVRKNGRDLLTTLGQPFVLNPNGNGLNQYRMISLTYMFSVDSESDVELGLPGRVFRQKLPEWTPNVQYYSSKEFSRLDHALHYNVRGTLALPVFNPSGQSCIGVVELIMTSEKIHYAPEVDKVCKALEAVNLKSSEILDHQTTQICNESRQNALAEILEVLTVVCETHNLPLAQTWVPCQHGSVLANGGGLKKNCTSFDGSCMGQICMSTTDMACYVVDAHVWGFRDACLEHHLQKGQGVAGRAFLNGGSCFCRDITKFCKTQYPLVHYALMFKLTTCFAISLQSSYTGDDSYILEFFLPSSITDDQEQDLLLGSILVTMKEHFQSLRVASGVDFGEDDDKLSFEIIQALPDKKVHSKIESIRVPFSGFKSNATETMLIPQPVVQSSDPVNEKINVATVNGVVKEKKKTEKKRGKTEKTISLDVLQQYFTGSLKDAAKSLGVCPTTMKRICRQHGISRWPSRKIKKVNRSITKLKRVIESVQGTDGGLDLTSMAVSSIPWTHGQTSAQPLNSPNGSKPPELPNTNNSPNHWSSDHSPNEPNGSPELPPSNGHKRSRTVDESAGTPTSHGSCDGNQLDEPKVPNQDPLFTVGGSPGLLFPPYSRDHDVSAASFAMPNRLLGSIDHFRGMLIEDAGSSKDLRNLCPTAAFDDKFQDTNWMNNDNNSNNNLYAPPKEEAIANVACEPSGSEMRTVTIKASYKDDIIRFRISSGSGIMELKDEVAKRLKVDAGTFDIKYLDDDNEWVLIACDADLQECLEIPRSSRTKIVRLLVHDVTTNLGSSCESTGELGSVSGWRLFKKIS*"
     CDS             116..142
                     /label="9xHis"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    185..3061
                     /label="AtNLP7 CDS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          608..637
                     /label="YMC152"
                     /note="sequence: GACAACTACTGTGTGATCAAAGAGAGGATG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2405..2430)
                     /label="YMC153"
                     /note="sequence: TCTAATTGGTTACCGTCACATGAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     tag             3068..3100
                     /label="HiBit"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(3108..3128)
                     /label="Gateway Recomb2"
                     /ApEinfo_revcolor="#dc143c"
                     /ApEinfo_fwdcolor="#dc143c"
     misc_recomb     complement(3108..3128)
                     /label="attB2"
                     /ApEinfo_revcolor="#2f4f4f"
                     /ApEinfo_fwdcolor="#2f4f4f"
     primer          complement(3171..3195)
                     /label="YMC155"
                     /note="sequence: ctcagcttcctttcgggctttgtta"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(3856..4671)
                     /label="Kan(R)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             4777..5396
                     /label="pBR322\origin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          5795..5817
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     misc_feature    complement(5811..6002)
                     /label="ROP"
                     /ApEinfo_revcolor="#dc143c"
                     /ApEinfo_fwdcolor="#dc143c"
     CDS             complement(6811..7902)
                     /label="lacI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 cgagatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
       61 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacca tgaaacacca
      121 ccaccaccac caccaccacc acggtggtct cgaatcaaca agtttGTACA AAAAAGCAGG
      181 CTTCATGTGC GAGCCCGATG ATAATTCCGC TAGAAACGGC GTCACTACTC AACCTTCGAG
      241 GTCAAGGGAG CTTCTAATGG ATGTTGACGA CTTAGATCTT GACGGTTCAT GGCCACTAGA
      301 TCAAATCCCT TACTTATCCT CATCGAATCG CATGATTTCT CCGATTTTTG TCTCCTCTTC
      361 CTCTGAGCAG CCTTGCTCGC CTCTCTGGGC TTTCTCCGAC GGTGGAGGAA ATGGTTTTCA
      421 CCACGCAACC TCCGGTGGCG ATGATGAGAA GATCAGCTCT GTCTCCGGTG TTCCTTCTTT
      481 CCGTCTCGCC GAGTATCCTC TCTTCCTCCC TTACTCTTCT CCATCAGCAG CTGAGAACAC
      541 AACAGAGAAG CATAACAGTT TCCAGTTTCC GTCTCCATTG ATGAGCCTAG TCCCACCAGA
      601 GAACACAGAC AACTACTGTG TGATCAAAGA GAGGATGACT CAGGCGCTTC GATACTTCAA
      661 AGAATCAACC GAACAACACG TTTTGGCTCA GGTCTGGGCT CCTGTGAGAA AGAATGGTCG
      721 TGATTTGCTG ACGACTTTGG GTCAACCTTT TGTTCTTAAT CCTAATGGTA ATGGGCTTAA
      781 TCAATACAGG ATGATCTCTC TCACATATAT GTTTTCTGTG GATAGTGAAA GTGACGTAGA
      841 GCTCGGACTC CCGGGTCGAG TTTTCCGTCA GAAATTGCCT GAATGGACTC CAAATGTTCA
      901 GTACTATTCC AGCAAAGAAT TCTCGCGGCT TGATCACGCC TTGCACTACA ACGTGCGTGG
      961 TACACTGGCC TTGCCTGTCT TTAATCCCTC TGGTCAGTCC TGCATAGGTG TTGTGGAACT
     1021 TATAATGACC TCAGAGAAGA TTCACTATGC ACCCGAAGTG GACAAAGTTT GCAAAGCCCT
     1081 TGAGGCGGTA AATCTGAAAA GCTCGGAAAT ACTTGATCAC CAAACAACAC AGATATGCAA
     1141 TGAGAGTCGC CAAAACGCGC TTGCTGAGAT TCTCGAAGTG CTGACAGTTG TATGTGAaAC
     1201 CCATAACTTG CCTCTCGCTC AGACTTGGGT TCCATGTCAG CATGGGAGCG TTCTTGCCAA
     1261 TGGTGGCGGT CTAAAGAAAA ACTGCACCAG CTTTGACGGT AGCTGCATGG GTCAAATCTG
     1321 CATGTCTACA ACCGACATGG CCTGCTATGT CGTGGATGCT CATGTCTGGG GCTTTAGAGA
     1381 TGCCTGTCTT GAACACCATC TCCAGAAAGG CCAGGGAGTC GCTGGACGAG CTTTTCTCAA
     1441 TGGTGGCTCA TGTTTCTGCA GAGACATCAC CAAGTTCTGC AAAACGCAGT ACCCACTAGT
     1501 CCATTATGCG CTCATGTTCA AGTTGACCAC TTGTTTTGCA ATATCTCTCC AGAGCTCTTA
     1561 CACGGGCGAC GACAGTTACA TTCTTGAATT TTTTCTTCCT TCGAGTATAA CAGACGACCA
     1621 AGAGCAAGAT TTGCTGTTGG GTTCTATTTT GGTGACAATG AAAGAACATT TTCAGAGTCT
     1681 GAGGGTTGCA TCTGGGGTTG ACTTTGGTGA AGATGACGAC AAATTGTCTT TCGAGATCAT
     1741 CCAAGCATTA CCGGACAAGA AGGTTCATTC AAAAATAGAA TCCATTCGAG TTCCCTTTTC
     1801 TGGTTTTAAG TCAAATGCAA CAGAGACGAT GTTGATTCCT CAGCCTGTGG TTCAGTCTTC
     1861 TGATCCAGTA AATGAGAAAA TCAACGTGGC CACTGTTAAC GGTGTGGTTA AGGAGAAGAA
     1921 GAAAACAGAG AAAAAGCGTG GGAAGACTGA GAAAACAATC AGTCTAGATG TACTTCAGCA
     1981 GTATTTCACT GGAAGTCTCA AAGACGCTGC AAAGAGCCTA GGAGTTTGCC CGACGACAAT
     2041 GAAGCGAATT TGCAGGCAAC ACGGAATCTC GCGGTGGCCA TCGAGGAAGA TCAAGAAAGT
     2101 GAATCGTTCA ATCACAAAGC TGAAACGAGT CATCGAATCT GTTCAAGGTA CTGATGGAGG
     2161 CCTCGACCTG ACTTCCATGG CCGTTAGTTC CATCCCTTGG ACACACGGTC AAACATCAGC
     2221 ACAGCCACTA AACTCACCCA ATGGTTCCAA ACCACCTGAG CTACCAAACA CCAATAATTC
     2281 ACCTAACCAT TGGTCAAGTG ATCACAGTCC GAACGAGCCA AATGGTTCGC CTGAGTTACC
     2341 ACCAAGCAAT GGTCACAAGC GATCACGAAC GGTGGATGAG AGCGCTGGGA CTCCAACCTC
     2401 TCATGGCTCA TGTGACGGTA ACCAATTAGA TGAACCGAAA GTCCCAAATC AAGATCCGCT
     2461 tTTCACGGTT GGTGGATCAC CCGGGCTCCT TTTTCCACCT TATTCTAGAG ATCATGATGT
     2521 ATCTGCAGCT TCCTTCGCAA TGCCGAACAG GCTTCTTGGT TCTATAGACC ATTTCCGAGG
     2581 AATGCTCATT GAAGACGCTG GAAGTTCAAA AGATCTGAGA AATCTCTGCC CCACTGCAGC
     2641 ATTTGACGAT AAGTTTCAAG ACACAAACTG GATGAACAAT GATAATAATA GCAACAACAA
     2701 CTTATACGCT CCCCCAAAGG AAGAGGCCAT TGCAAATGTT GCATGCGAAC CATCAGGCTC
     2761 AGAAATGAGA ACGGTAACAA TCAAAGCAAG TTACAAAGAC GACATAATAC GGTTCAGAAT
     2821 ATCCTCGGGT TCAGGTATAA TGGAATTGAA GGATGAAGTG GCTAAGAGGC TGAAAGTTGA
     2881 TGCAGGAACG TTCGATATCA AGTATCTTGA CGATGATAAC GAATGGGTTT TAATAGCTTG
     2941 TGATGCTGAT CTTCAAGAAT GTCTCGAGAT CCCTAGATCC TCCCGCACGA AAATCGTAAG
     3001 GCTCTTAGTT CATGATGTAA CGACAAATCT AGGGAGCTCC TGCGAGAGCA CTGGAGAATT
     3061 GggttcgGTG AGCGGCTGGC GGCTGTTCAA GAAGATTAGC TGAAACCCAG CTTTCTTGTA
     3121 Caaagtggtt gattcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
     3181 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     3241 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
     3301 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3361 tgaccgctac accttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3421 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3481 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3541 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3601 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3661 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3721 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcaggtgg cacttttcgg
     3781 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3841 ctcatgaatt aattcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     3901 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     3961 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     4021 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     4081 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     4141 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     4201 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
     4261 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     4321 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
     4381 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     4441 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     4501 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
     4561 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     4621 ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca taacacccct
     4681 tgtattactg tttatgtaag cagacagttt tattgttcat gaccaaaatc ccttaacgtg
     4741 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4801 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4861 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4921 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4981 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5041 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5101 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5161 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5221 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5281 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5341 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5401 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5461 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5521 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     5581 ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat
     5641 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     5701 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5761 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5821 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     5881 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     5941 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     6001 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     6061 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     6121 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     6181 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     6241 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     6301 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     6361 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     6421 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     6481 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     6541 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     6601 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     6661 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     6721 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     6781 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6841 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6901 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     6961 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     7021 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     7081 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     7141 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     7201 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     7261 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     7321 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     7381 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     7441 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     7501 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     7561 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     7621 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     7681 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     7741 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     7801 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     7861 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     7921 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     7981 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     8041 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     8101 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     8161 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     8221 cggtgatgcc ggccacgatg cgtccggcgt agaggat
//