LOCUS       pEPYCdKN0012            6139 bp ds-DNA     circular     09-DEC-2022
DEFINITION  .
COMMENT     pH9GW_9xHis_AtNAC032_HiBit GateWay(tm) LR reaction between
            pENTR_AtNAC032_HiBit.xdna as an Entry vector and ph9gw.gb.xdna as a
            Destination vector.
FEATURES             Location/Qualifiers
     CDS             complement(593..1684)
                     /label="lacI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        2062..2078
                     /label="T7\Promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2062..2081
                     /label="T7\Primer"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     misc_feature    2135..2141
                     /label="RBS"
                     /ApEinfo_revcolor="#dc143c"
                     /ApEinfo_fwdcolor="#dc143c"
     CDS             2149..3024
                     /label="Translation 2149-3024"
                     /translation="MKHHHHHHHHHGGLESTSLYKKAGFMMKSGADLQFPPGFRFHPTDEELVLMYLCRKCASQPIPAPIITELDLYRYDPWDLPDMALYGEKEWYFFSPRDRKYPNGSRPNRAAGTGYWKATGADKPIGRPKPVGIKKALVFYSGKPPNGEKTNWIMHEYRLADVDRSVRKKNSLRLDDWVLCRIYNKKGVIEKRRSDIEDGLKPVTDTCPPESVARLISGSEQAVSPEFTCSNGRLSNALDFPFNYVDAIADNEIVSRLLGGNQMWSTTLDPLVVRQGTFGSVSGWRLFKKIS*"
     CDS             2155..2181
                     /label="9xHis"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2224..2982
                     /label="ANAC032 CDS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     tag             2989..3021
                     /label="HiBit"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_recomb     complement(3029..3049)
                     /label="attB2"
                     /ApEinfo_revcolor="#2f4f4f"
                     /ApEinfo_fwdcolor="#2f4f4f"
     misc_feature    complement(3029..3049)
                     /label="Gateway Recomb2"
                     /ApEinfo_revcolor="#dc143c"
                     /ApEinfo_fwdcolor="#dc143c"
     CDS             complement(3777..4592)
                     /label="Kan(R)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             4698..5317
                     /label="pBR322\origin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          5716..5738
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     misc_feature    complement(5732..5923)
                     /label="ROP"
                     /ApEinfo_revcolor="#dc143c"
                     /ApEinfo_fwdcolor="#dc143c"
ORIGIN
        1 gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt
       61 ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt
      121 cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg
      181 ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat
      241 gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt
      301 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
      361 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc
      421 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat
      481 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
      541 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc
      601 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      661 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca
      721 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
      781 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
      841 tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact
      901 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
      961 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
     1021 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
     1081 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
     1141 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
     1201 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
     1261 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
     1321 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta
     1381 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
     1441 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
     1501 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
     1561 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
     1621 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
     1681 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
     1741 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag
     1801 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
     1861 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
     1921 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
     1981 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
     2041 gagatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
     2101 attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat gaaacaccac
     2161 caccaccacc accaccacca cggtggtctc gaatcaacaa gtttGTACAA AAAAGCAGGC
     2221 TTCATGATGA AATCTGGGGC TGATTTGCAA TTTCCACCAG GATTTAGATT TCATCCTACG
     2281 GATGAGGAGC TAGTCCTCAT GTATCTCTGT CGTAAATGCG CGTCGCAGCC GATCCCTGCT
     2341 CCGATTATCA CCGAACTCGA TTTGTACCGA TATGATCCTT GGGACCTTCC CGACATGGCT
     2401 TTGTACGGTG AAAAGGAGTG GTATTTTTTC TCACCAAGAG ATCGAAAGTA TCCAAACGGT
     2461 TCAAGACCCA ACCGTGCAGC TGGTACTGGA TATTGGAAAG CTACCGGAGC TGATAAACCA
     2521 ATAGGTCGTC CTAAACCGGT TGGTATTAAG AAGGCTCTAG TGTTTTACTC GGGAAAACCT
     2581 CCAAATGGAG AGAAAACCAA TTGGATTATG CACGAATACC GGCTCGCTGA CGTTGACCGG
     2641 TCGGTTCGTA AGAAAAACAG TCTAAGATTG GACGATTGGG TATTGTGTCG TATATATAAC
     2701 AAGAAAGGTG TCATCGAGAA GCGACGAAGC GATATCGAGG ACGGGTTAAA GCCTGTGACT
     2761 GACACGTGTC CACCGGAATC TGTGGCGAGA TTGATCTCCG GCTCGGAGCA AGCGGTGTCA
     2821 CCGGAATTCA CGTGTAGCAA CGGTCGGTTG AGTAATGCCC TTGATTTTCC GTTTAATTAC
     2881 GTAGATGCCA TCGCCGATAA CGAGATTGTG TCACGGCTAT TGGGCGGGAA TCAGATGTGG
     2941 TCGACGACGC TTGATCCACT TGTGGTTAGG CAGGGAACTT TCggttcgGT GAGCGGCTGG
     3001 CGGCTGTTCA AGAAGATTAG CTGAAACCCA GCTTTCTTGT ACaaagtggt tgattcgagc
     3061 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     3121 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     3181 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
     3241 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta caccttgcca
     3301 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3361 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3421 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3481 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3541 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3601 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3661 acaaaatatt aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3721 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag
     3781 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     3841 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     3901 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     3961 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     4021 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     4081 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     4141 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     4201 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     4261 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     4321 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     4381 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     4441 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     4501 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
     4561 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     4621 gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4861 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5341 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5401 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5461 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     5521 cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5581 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     5641 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5701 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5761 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg
     5821 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     5881 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     5941 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     6001 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
     6061 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
     6121 cagatgtagg tgttccaca
//