LOCUS       pEPYCdKN0022            7186 bp ds-DNA     circular     09-DEC-2022
DEFINITION  .
COMMENT     pH9GW_9xHis_AtARF18_HiBit GateWay(tm) LR reaction between
            pENTR_AtARF18_HiBit.xdna as an Entry vector and ph9gw.gb.xdna as a
            Destination vector.
FEATURES             Location/Qualifiers
     promoter        23..39
                     /label="T7\Promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          23..42
                     /label="T7\Primer"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     misc_feature    96..102
                     /label="RBS"
                     /ApEinfo_revcolor="#dc143c"
                     /ApEinfo_fwdcolor="#dc143c"
     CDS             116..142
                     /label="9xHis"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             185..1990
                     /label="ARF18_CDS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     tag             1997..2029
                     /label="HiBit"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_recomb     complement(2037..2057)
                     /label="attB2"
                     /ApEinfo_revcolor="#2f4f4f"
                     /ApEinfo_fwdcolor="#2f4f4f"
     misc_feature    complement(2037..2057)
                     /label="Gateway Recomb2"
                     /ApEinfo_revcolor="#dc143c"
                     /ApEinfo_fwdcolor="#dc143c"
     CDS             complement(2785..3600)
                     /label="Kan(R)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3706..4325
                     /label="pBR322\origin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          4724..4746
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_fwdcolor="#808000"
     misc_feature    complement(4740..4931)
                     /label="ROP"
                     /ApEinfo_revcolor="#dc143c"
                     /ApEinfo_fwdcolor="#dc143c"
     CDS             complement(5740..6831)
                     /label="lacI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 cgagatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
       61 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacca tgaaacacca
      121 ccaccaccac caccaccacc acggtggtct cgaatcaaca agtttGTACA AAAAAGCAGG
      181 CTTCATGGCG AGTGTTGAAG GTGATGATGA TTTCGGAAGT TCTTCGTCAA GGTCTTATCA
      241 AGATCAACTA TACACAGAGC TATGGAAAGT TTGTGCAGGT CCATTAGTGG AAGTTCCTCG
      301 TGCTCAAGAG AGAGTTTTCT ACTTCCCTCA GGGTCACATG GAACAACTTG TGGCGTCAAC
      361 TAATCAAGGA ATCAATTCAG AAGAAATACC TGTTTTTGAT CTTCCTCCAA AGATACTTTG
      421 TCGAGTTCTT GATGTCACTT TAAAGGCGGA GCATGAAACA GATGAGGTTT ACGCTCAGAT
      481 CACATTACAA CCAGAGGAAG ATCAAAGTGA ACCAACAAGT CTTGATCCAC CTATTGTTGG
      541 ACCAACTAAG CAAGAGTTTC ATTCGTTTGT TAAGATTTTA ACGGCTTCAG ATACAAGCAC
      601 TCATGGTGGA TTCTCTGTTC TTCGTAAACA CGCCACTGAA TGCTTGCCTT CTTTGGATAT
      661 GACACAAGCT ACTCCTACTC AAGAACTTGT GACTAGAGAT CTTCATGGCT TTGAATGGAG
      721 GTTTAAGCAT ATATTCAGAG GACAACCACG GAGGCATTTG CTTACTACGG GTTGGAGTAC
      781 ATTTGTATCC TCGAAAAGAC TTGTAGCTGG AGATGCTTTT GTGTTCTTGA GGGGTGAGAA
      841 TGGGGATTTA CGGGTTGGAG TGAGACGATT AGCTCGGCAT CAAAGCACAA TGCCTACTTC
      901 GGTTATTTCA AGTCAGAGCA TGCATTTGGG AGTTCTTGCT ACAGCTTCTC ATGCTGTGCG
      961 TACAACAACA ATCTTTGTTG TCTTTTACAA GCCTAGGATA AGCCAATTCA TAGTTGGGGT
     1021 GAACAAGTAT ATGGAAGCTA TAAAGCATGG ATTTTCTCTC GGTACCCGAT TCAGAATGAG
     1081 GTTTGAAGGA GAAGAGTCTC CTGAGAGAAT ATTTACTGGT ACGATTGTGG GAAGTGGAGA
     1141 TCTATCTTCA CAATGGCCAG CTTCTAAATG GAGGTCATTG CAGGTACAAT GGGATGAGCC
     1201 AACAACAGTT CAaagaCCAG ATAAAGTCTC ACCATGGGAG ATAGAGCCTT TCTTGGCAAC
     1261 TTCCCCAATT TCAACTCCTG CTCAACAACC ACAATCGAAA TGCAAGCGGT CAAGACCCAT
     1321 CGAGCCATCA GTTAAAACAC CAGCCCCACC TAGTTTCTTG TACAGCCTCC CTCAGAGCCA
     1381 AGATTCCATT AATGCATCCC TTAAACTGTT TCAAGATCCA TCACTTGAGA GAATTTCAGG
     1441 TGGATACTCC TCAAACAACA GCTTCAAACC CGAGACTCCT CCTCCTCCAA CGAATTGTAG
     1501 CTATAGGTTG TTTGGATTTG ATCTCACAAG CAATTCTCCT GCTCCAATCC CTCAAGACAA
     1561 GCAACCGATG GATACTTGTG GAGCTGCCAA GTGTCAAGAA CCCATCACTC CAACCTCAAT
     1621 GAGTGAGCAG AAGAAGCAAC AAACATCAAG AAGTCGAACT AAAGTGCAAA TGCAAGGCAT
     1681 TGCGGTTGGT CGTGCGGTTG ATTTAACACT GTTGAAATCT TACGATGAAC TGATTGATGA
     1741 GCTTGAGGAG ATGTTTGAGA TTCAAGGACA GCTTCTTGCC CGAGACAAAT GGATCGTTGT
     1801 CTTCACTGAT GATGAAGGAG ATATGATGCT TGCTGGTGAT GATCCGTGGA ATGAGTTTTG
     1861 CAAGATGGCA AAGAAGATAT TTATATATTC GAGCGATGAG GTTAAGAAAA TGACAACGAA
     1921 ACTGAAGATT TCTTCGTCGT TAGAGAATGA GGAATATGGT AATGAATCAT TCGAAAATCG
     1981 TAGTAGGGGG ggttcgGTGA GCGGCTGGCG GCTGTTCAAG AAGATTAGCT GAAACCCAGC
     2041 TTTCTTGTAC aaagtggttg attcgagcac caccaccacc accactgaga tccggctgct
     2101 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     2161 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     2221 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2281 cgcgcagcgt gaccgctaca ccttgccagc gccctagcgc ccgctccttt cgctttcttc
     2341 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2401 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     2461 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2521 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     2581 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     2641 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc
     2701 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2761 atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa
     2821 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     2881 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     2941 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     3001 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
     3061 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     3121 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
     3181 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     3241 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     3301 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     3361 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     3421 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     3481 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     3541 atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat
     3601 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc
     3661 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3841 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     4501 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct
     4561 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     4621 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4681 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4741 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     4801 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     4861 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     4921 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     4981 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     5041 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     5101 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
     5161 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     5221 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     5281 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     5341 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggg
     5401 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg
     5461 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
     5521 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
     5581 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc
     5641 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     5701 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5761 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5821 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     5881 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     5941 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     6001 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
     6061 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     6121 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     6181 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     6241 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     6301 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     6361 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     6421 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     6481 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     6541 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     6601 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     6661 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     6721 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     6781 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     6841 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
     6901 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     6961 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     7021 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     7081 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     7141 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggat
//