LOCUS       Exported                8553 bp DNA     circular SYN 03-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Biomedical Sciences
  TITLE     Direct Submission
  JOURNAL   Exported Dec 22, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8553
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    1..515
                     /label=5'LTR
                     /note="this is apparently the promoter"
     primer_bind     293..312
                     /label=MMLV-Fwd seq primer
                     /note="seq primer"
     misc_feature    579..920
                     /label=MESV psi
     misc_feature    654..1403
                     /label=Psi Plus Pack
     misc_feature    990..1397
                     /label=Gag
     primer_bind     1333..1355
                     /label=MSCV Seq Primer QB0085
                     /note="MSCV Fwd sequencing primer"
     primer_bind     1371..1387
                     /label=pBABE 5' Fwd seq primer
                     /note="Psi packaging signal, 5' of MCS in pBABE vectors, 
                     forward primer"
     misc_feature    1410..1428
                     /label=MCS
     misc_feature    1416..1421
                     /label=XhoI site
     primer_bind     complement(1421..1440)
                     /label=MSCV Amp Rev1
     misc_feature    1421..1427
                     /label=HpaI site
     primer_bind     1476..1520
                     /label=AttB1_CD16
     protein_bind    1476..1500
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1509..2273
                     /codon_start=1
                     /label=CD16
                     /translation="MWQLLLPTALLLLVSAGMRTEDLPKAVVFLEPQWYRVLEKDSVTL
                     KCQGAYSPEDNSTQWFHNESLISSQASSYFIDAATVDDSGEYRCQTNLSTLSDPVQLEV
                     HIGWLLLQAPRWVFKEEDPIHLRCHSWKNTALHKVTYLQNGKGRKYFHHNSDFYIPKAT
                     LKDSGSYFCRGLVGSKNVSSETVNITITQGLAVSTISSFFPPGYQVSFCLVMVLLFAVD
                     TGLYFSVKTNIRSSTRDWKDHKFKWRKDPQDK"
     misc_feature    2034..2036
                     /label=high affinity variant 158V
     primer_bind     complement(2248..2299)
                     /label=Attb2_CD16
     primer_bind     2271..2295
                     /label=Gibson pDONRbackbone fwd
     protein_bind    complement(2275..2299)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2310..2333
                     /label=Flag Tag
     misc_feature    2337..2360
                     /label=Flag Tag
     misc_feature    2445..2843
                     /label=mPGK Promoter
     primer_bind     2766..2785
                     /label=mPGK-F Seq Primer
                     /note="seq primer"
     misc_feature    2854..3453
                     /label=PuroR
     misc_feature    3519..4022
                     /label=IRES
     primer_bind     complement(3542..3561)
                     
                     /note="seq primer for OptiVECT Topo Vector"
     primer_bind     complement(3603..3622)
                     
                     /note="seq primer"
     primer_bind     3902..3921
                     /label=IRES-F 3' Fwd seq primer
                     /note="seq primer"
     misc_feature    4022..4081
                     /label=Extended IRES according to Clontech
     misc_feature    4094..4810
                     /label=eGFP
     primer_bind     complement(4139..4160)
                     /label=EGFP-N 5' Rev Seq primer
                     /note="seq primer"
     primer_bind     complement(4400..4419)
                     /label=EXFP-R Rev seq primer
                     /note="distinguishing seq primer"
     primer_bind     4747..4768
                     /label=EGFP-C 3' Fwd seq primer
                     /note="seq primer"
     misc_feature    4915..5428
                     /label=3'LTR
     primer_bind     5208..5227
                     /label=MMLV-Fwd seq primer
                     /note="seq primer"
     primer_bind     complement(5597..5614)
                     /label=M13/pUC rev Primer QB0048
                     /note="seq primer"
     primer_bind     complement(5611..5633)
                     /label=M13 Reverse (-48) / M13 pUC rev
     misc_feature    complement(6759..7619)
                     /label=AmpR
     primer_bind     7382..7401
                     
                     /note="sequencing primer for amp resistance gene chk"
     primer_bind     complement(7848..7870)
                     
                     /note="3' of MCS in pGEX vectors, reverse primer"
     misc_feature    complement(8069..8200)
                     /label=LacZ_A
     primer_bind     8183..8205
                     /label=M13/pUC Fwd
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaacgaa ttctaccggg
     1441 tatagggaga cccaagctgg ctagttaagc tatcaacaag tttgtacaaa aaagcaggct
     1501 tcgccaccat gtggcagctg ctcctcccaa ctgctctgct acttctagtt tcagctggca
     1561 tgcggactga agatctccca aaggctgtgg tgttcctgga gcctcaatgg tacagggtgc
     1621 tcgagaagga cagtgtgact ctgaagtgcc agggagccta ctcccctgag gacaattcca
     1681 cacagtggtt tcacaatgag agcctcatct caagccaggc ctcgagctac ttcattgacg
     1741 ctgccacagt cgacgacagt ggagagtaca ggtgccagac aaacctctcc accctcagtg
     1801 acccggtgca gctagaagtc catatcggct ggctgttgct ccaggcccct cggtgggtgt
     1861 tcaaggagga agaccctatt cacctgaggt gtcacagctg gaagaacact gctctgcata
     1921 aggtcacata tttacagaat ggcaaaggca ggaagtattt tcatcataat tctgacttct
     1981 acattccaaa agccacactc aaagacagcg gctcctactt ctgcaggggg cttgtgggga
     2041 gtaaaaatgt gtcttcagag actgtgaaca tcaccatcac tcaaggtttg gcagtgtcaa
     2101 ccatctcatc attctttcca cctgggtacc aagtctcttt ctgcttggtg atggtactcc
     2161 tttttgcagt ggacacagga ctatatttct ctgtgaagac aaacattcga agctcaacaa
     2221 gagactggaa ggaccataaa tttaaatgga gaaaggaccc tcaagacaaa tgagacccag
     2281 ctttcttgta caaagtggtt gatctagagg attacaagga tgacgacgat aagggagatt
     2341 acaaggatga cgacgataag tgatctagag ggcccgcggt tcgaaggtaa gcctatcttg
     2401 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     2461 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     2521 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     2581 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     2641 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     2701 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     2761 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     2821 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     2881 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     2941 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     3001 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     3061 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     3121 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     3181 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     3241 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     3301 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     3361 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     3421 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     3481 accgaaagga gcgcacgacc ccatgcatcc aattccgccc ccccccccta acgttactgg
     3541 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     3601 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     3661 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     3721 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     3781 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     3841 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     3901 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     3961 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     4021 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     4081 tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4141 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4201 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4261 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4321 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4381 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4441 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4501 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4561 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     4621 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4681 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4741 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4801 gctgtacaag taatgaatta attaagaatt atcaagctta tcgataccgt cgacctgcag
     4861 ccaagcttat cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     4921 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
     4981 atacataact gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata
     5041 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     5101 tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg
     5161 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
     5221 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
     5281 tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtgtatc caataaaccc
     5341 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     5401 ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt ggagaacaac
     5461 attctgaggg taggagtcga atattaagta atcctgactc aattagccac tgttttgaat
     5521 ccacatactc caatactcct gaaatagttc attatggaca gcgcagaaga gctggggaga
     5581 attaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5641 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5701 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5761 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5821 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5881 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5941 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6001 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6061 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6181 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6301 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6361 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6421 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6481 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6541 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6601 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6661 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6721 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6781 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6841 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6901 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6961 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7021 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7081 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7141 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7201 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7261 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7321 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7381 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7441 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7501 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7561 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7621 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7681 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7741 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7801 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7861 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7921 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     7981 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     8041 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     8101 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     8161 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc gcaaggaatg
     8221 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     8281 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     8341 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     8401 cggcgtagag gcgattagtc caatttgtta aagacaggat atcagtggtc caggctctag
     8461 ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa
     8521 agattttatt tagtctccag aaaaaggggg gaa
//
