LOCUS       pRM055_03              9111 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3127..7229
                     /note="pLannotate"
                     /label="Cas9"
                     /database="addgene"
                     /identity="99.927"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             8081..8941
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1846..2746)
                     /note="pLannotate"
                     /label="TcR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="75.65071368597816"
                     /fragment="True"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1016..1207)
                     /note="pLannotate"
                     /label="rop"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    775..914
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.592"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     promoter        7976..8080
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(7495..7787)
                     /note="pLannotate"
                     /label="TcR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="24.601175482787575"
                     /fragment="True"
                     /other="CDS"
     terminator      7422..7469
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        complement(7835..7863)
                     /note="pLannotate"
                     /label="tet promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     RBS             2875..2897
                     /note="pLannotate"
                     /label="RBS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     CDS             3049..3069
                     /note="pLannotate"
                     /label="SV40 NLS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2989..3009
                     /note="pLannotate"
                     /label="SV40 NLS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3079..3099
                     /note="pLannotate"
                     /label="SV40 NLS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3019..3039
                     /note="pLannotate"
                     /label="SV40 NLS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2935..2955
                     /note="pLannotate"
                     /label="TEV site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2959..2979
                     /note="pLannotate"
                     /label="TEV site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7365..7414
                     /note="pLannotate"
                     /label="SET1"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="39.682539682539684"
                     /fragment="True"
                     /other="CDS"
     promoter        2768..2786
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             2916..2934
                     /note="pLannotate"
                     /label="7xHis"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="90.47619047619048"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     1681 acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     1741 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     1801 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     1861 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     1921 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     1981 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     2041 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     2101 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     2161 gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     2221 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     2281 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     2341 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     2401 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     2461 catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     2521 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     2581 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     2641 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     2701 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
     2761 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag tgccggctcc
     2821 ggagagctct ttaattaagc ggccgccctg caggactcga gttctagaaa taattttgtt
     2881 taactttaag aaggagatat acatatgaaa tcttctcacc atcaccatca ccatgaaaac
     2941 ctgtacttcc aatccaatga aaacctgtac ttccaatcca atgccactcc caagaagaag
     3001 cgaaaggtgg gtgggtcccc aaagaagaaa cggaaagtag gcggctcccc caaaaagaag
     3061 cgaaaagtag ggggtagccc caagaagaag cggaaggtag gtatccacgg agtcccagca
     3121 gctaccatgg ataagaaata ctcaataggc ttagatatcg gcacaaatag cgtcggatgg
     3181 gcggtgatca ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca
     3241 gaccgccaca gtatcaaaaa aatcttatag gggctctttt atttgacagt ggagagacag
     3301 cggaagcgac tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg aagaatcgta
     3361 ttagttatct acaggagatt ttttcaaatg agatggcgaa agtagatgat agtttctttc
     3421 atcgacttga agagtctttt ttggtggaag aagacaagaa gcatgaacgt catcctattt
     3481 ttggaaatat agtagatgaa gttgcttatc atgagaaata tccaactatc tatcatctgc
     3541 gaaaaaaatt ggtagattct actgataaag cggatttgcg cttaatctat ttggccttag
     3601 cgcatatgat taagtttcgt ggtcattttt tgattgaggg agatttaaat cctgataata
     3661 gtgatgtgga caaactattt atccagttgg tacaaaccta caatcaatta tttgaagaaa
     3721 accctattaa cgcaagtgga gtagatgcta aagcgattct ttctgcacga ttgagtaaat
     3781 caagacgatt agaaaatctc attgctcagc tccccggtga gaagaaaaat ggcttatttg
     3841 ggaatctcat tgctttgtca ttgggtttga cccctaattt taaatcaaat tttgatttgg
     3901 cagaagatgc taaattacag ctttcaaaag atacttacga tgatgattta gataatttat
     3961 tggcgcaaat tggagatcaa tatgctgatt tgtttttggc agctaagaat ttatcagatg
     4021 ctattttact ttcagatatc ctaagagtaa atactgaaat aactaaggct cccctatcag
     4081 cttcaatgat taaacgctac gatgaacatc atcaagactt gactctttta aaagctttag
     4141 ttcgacaaca acttccagaa aagtataaag aaatcttttt tgatcaatca aaaaacggat
     4201 atgcaggtta tattgatggg ggagctagcc aagaagaatt ttataaattt atcaaaccaa
     4261 ttttagaaaa aatggatggt actgaggaat tattggtgaa actaaatcgt gaagatttgc
     4321 tgcgcaagca acggaccttt gacaacggct ctattcccca tcaaattcac ttgggtgagc
     4381 tgcatgctat tttgagaaga caagaagact tttatccatt tttaaaagac aatcgtgaga
     4441 agattgaaaa aatcttgact tttcgaattc cttattatgt tggtccattg gcgcgtggca
     4501 atagtcgttt tgcatggatg actcggaagt ctgaagaaac aattacccca tggaattttg
     4561 aagaagttgt cgataaaggt gcttcagctc aatcatttat tgaacgcatg acaaactttg
     4621 ataaaaatct tccaaatgaa aaagtactac caaaacatag tttgctttat gagtatttta
     4681 cggtttataa cgaattgaca aaggtcaaat atgttactga aggaatgcga aaaccagcat
     4741 ttctttcagg tgaacagaag aaagccattg ttgatttact cttcaaaaca aatcgaaaag
     4801 taaccgttaa gcaattaaaa gaagattatt tcaaaaaaat agaaagtttt gatagtgttg
     4861 aaatttcagg agttgaagat agatttaatg cttcattagg tacctaccat gatttgctaa
     4921 aaattattaa agataaagat tttttggata atgaagaaaa tgaagatatc ttagaggata
     4981 ttgttttaac attgacctta tttgaagata gggagatgat tgaggaaaga cttaaaacat
     5041 atgctcacct ctttgatgat aaggtgatga aacagcttaa acgtcgccgt tatactggtt
     5101 ggggacgttt gtctcgaaaa ttgattaatg gtattaggga taagcaatct ggcaaaacaa
     5161 tattagattt tttgaaatca gatggttttg ccaatcgcaa ttttatgcag ctgatccatg
     5221 atgatagttt gacatttaaa gaagacattc aaaaagcaca agtgtctgga caaggcgata
     5281 gtttacatga acatattgca aatttagctg gtagccctgc tattaaaaaa ggtattttac
     5341 agactgtaaa agttgttgat gaattggtca aagtaatggg gcggcataag ccagaaaata
     5401 tcgttattga aatggcacgt gaaaatcaga caactcaaaa gggccagaaa aattcgcgag
     5461 agcgtatgaa acgaatcgaa gaaggtatca aagaattagg aagtcagatt cttaaagagc
     5521 atcctgttga aaatactcaa ttgcaaaatg aaaagctcta tctctattat ctccaaaatg
     5581 gaagagacat gtatgtggac caagaattag atattaatcg tttaagtgat tatgatgtcg
     5641 atcacattgt tccacaaagt ttccttaaag acgattcaat agacaataag gtcttaacgc
     5701 gttctgataa aaatcgtggt aaatcggata acgttccaag tgaagaagta gtcaaaaaga
     5761 tgaaaaacta ttggagacaa cttctaaacg ccaagttaat cactcaacgt aagtttgata
     5821 atttaacgaa agctgaacgt ggaggtttga gtgaacttga taaagctggt tttatcaaac
     5881 gccaattggt tgaaactcgc caaatcacta agcatgtggc acaaattttg gatagtcgca
     5941 tgaatactaa atacgatgaa aatgataaac ttattcgaga ggttaaagtg attaccttaa
     6001 aatctaaatt agtttctgac ttccgaaaag atttccaatt ctataaagta cgtgagatta
     6061 acaattacca tcatgcccat gatgcgtatc taaatgccgt cgttggaact gctttgatta
     6121 agaaatatcc aaaacttgaa tcggagtttg tctatggtga ttataaagtt tatgatgttc
     6181 gtaaaatgat tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt
     6241 actctaatat catgaacttc ttcaaaacag aaattacact tgcaaatgga gagattcgca
     6301 aacgccctct aatcgaaact aatggggaaa ctggagaaat tgtctgggat aaagggcgag
     6361 attttgccac agtgcgcaaa gtattgtcca tgccccaagt caatattgtc aagaaaacag
     6421 aagtacagac aggcggattc tccaaggagt caattttacc aaaaagaaat tcggacaagc
     6481 ttattgctcg taaaaaagac tgggatccaa aaaaatatgg tggttttgat agtccaacgg
     6541 tagcttattc agtcctagtg gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat
     6601 ccgttaaaga gttactaggg atcacaatta tggaaagaag ttcctttgaa aaaaatccga
     6661 ttgacttttt agaagctaaa ggatataagg aagttaaaaa agacttaatc attaaactac
     6721 ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg gatgctggct agtgccggag
     6781 aattacaaaa aggaaatgag ctggctctgc caagcaaata tgtgaatttt ttatatttag
     6841 ctagtcatta tgaaaagttg aagggtagtc cagaagataa cgaacaaaaa caattgtttg
     6901 tggagcagca taagcattat ttagatgaga ttattgagca aatcagtgaa ttttctaagc
     6961 gtgttatttt agcagatgcc aatttagata aagttcttag tgcatataac aaacatagag
     7021 acaaaccaat acgtgaacaa gcagaaaata ttattcattt atttacgttg acgaatcttg
     7081 gagctcccgc tgcttttaaa tattttgata caacaattga tcgtaaacga tatacgtcta
     7141 caaaagaagt tttagatgcc actcttatcc atcaatccat cactggtctt tatgaaacac
     7201 gcattgattt gagtcagcta ggaggtgacg ggtcacctaa gaaaaaacga aaagttgagg
     7261 atcctaaaaa gaaacgaaaa gtttaaattg gaagtggata acggatccgc gatcgcggcg
     7321 cgccacctgg tggccggccg gtaccacgcg tgcgcgctga tccggctgct aacaaagccc
     7381 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     7441 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac
     7501 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc
     7561 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat
     7621 tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg
     7681 acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
     7741 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
     7801 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt
     7861 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7921 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7981 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8041 accctgataa atgcttcaat aacattgaaa aaggaagagt atgagtattc aacatttccg
     8101 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8161 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8221 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8281 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     8341 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8401 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8461 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8521 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8581 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     8641 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8701 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8761 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8821 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8881 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8941 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9001 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9061 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
