LOCUS       pMK33-GW-turboID       10983 bp DNA     linear   SYN 31-DEC-2022
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 31, 2022 from SnapGene 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      6385..6419
                     /label=SV40 early polyA
                     /note="SV40 early polyA"
     misc_feature    complement(6459..6488)
                     /label=Gibson Reverse
                     /note="Gibson Reverse"
     misc_feature    6489..6915
                     /label=complete MT promoter
                     /note="complete MT promoter"
     promoter        6493..6838
                     /label=MT promoter
                     /note="MT promoter"
     misc_feature    6735..6754
                     /label=MTseq2F
                     /note="MTseq2F"
     misc_feature    6839..6856
                     /label=Gibson Forward
                     /note="Gibson Forward"
     misc_feature    6896..6913
                     /label=MTseq1F
                     /note="MTseq1F"
     misc_feature    6930..8649
                     /label=GW
                     /note="GW"
     misc_signal     6943..7065
                     /label=attR1
                     /note="attR1"
     misc_feature    6949..8637
                     /label=paste entry here
                     /note="paste entry here"
     misc_feature    complement(7097..7116)
                     /label=GWseq_1R
                     /note="GWseq_1R"
     misc_feature    8508..8527
                     /label=GWseq1F
                     /note="GWseq1F"
     misc_feature    8626..8652
                     /label=misc_feature 2
     CDS             8680..9636
                     /codon_start=1
                     /label=1
                     /note="BirA (humanized)"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    8680..8717
                     /label=misc_feature 3
     misc_feature    8680..8702
                     /label=misc_feature 1
     misc_feature    8869..8871
                     /label=Q65P
                     /note="Q65P"
     misc_feature    8935..8937
                     /label=I87V
                     /note="I87V"
     misc_feature    9019..9021
                     /label=G115G
                     /note="G115G"
     misc_feature    9028..9030
                     /label=R118S
                     /note="R118S"
     misc_feature    9094..9096
                     /label=E141K
                     /note="E141K"
     misc_difference 9097..9099
                     /label=Q142R
                     /note="Q142R"
     misc_feature    9121..9123
                     /label=S150G
                     /note="S150G"
     misc_feature    9124..9126
                     /label=L151P
                     /note="L151P"
     misc_feature    9151..9153
                     /label=V160A
                     /note="V160A"
     misc_feature    9247..9249
                     /label=T192A
                     /note="T192A"
     misc_difference 9253..9255
                     /label=K194I
                     /note="K194I"
     misc_feature    9298..9300
                     /label=M209V
                     /note="M209V"
     misc_feature    9394..9396
                     /label=M241T
                     /note="M241T"
     misc_feature    9460..9462
                     /label=S263P
                     /note="S263P"
     misc_structure  9544..9546
                     /label=G291G
                     /note="G291G"
     misc_feature    9586..9588
                     /label=I305V
                     /note="I305V"
     misc_feature    9643..9684
                     /label=V5 tag
                     /note="V5 tag"
     misc_feature    complement(9924..9943)
                     /label=pAct_seq1R
                     /note="pAct_seq1R"
ORIGIN
        1 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
       61 ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg
      121 atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta
      181 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
      241 atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
      301 tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg
      361 ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc
      421 gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt
      481 agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg
      541 cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc
      601 ggaagtcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      661 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      721 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      781 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      841 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      901 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      961 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1021 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1081 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1141 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1201 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     1261 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1321 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1381 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1441 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1501 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1561 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1621 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1681 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1741 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1801 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1861 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1921 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1981 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2041 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2101 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2161 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2221 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2281 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2341 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2401 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2461 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2521 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2581 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattcacatt tgtacgaatt
     2641 ttttttttat caaaagttcg agtttttcac caatttcctc atcaaccgag caaggcaaac
     2701 ggctttgaat aatatggtgt tatatataca tatatcaaat cgctgctgac tgcgtgattg
     2761 atggccccaa gattacatat tatcgaatca ggattcagaa ggagatcaat gtcaaatgcg
     2821 gacaggaaca tgaaagacgc ctgttatgcg caattaaaaa tttgggttta attgctgtgg
     2881 aaactgttgt tggcggcatc ttaagttcct gtttaacaac atcaactact tatgtacgta
     2941 gaagcgttta agccatttgc atacagatga gaactggctt ttgtgctaat cagtcaagat
     3001 gactccgatg atgatgactc attacctgac cagttttcgc tgctttcttt tcaacaacta
     3061 cttgtatatg tattgtatcc aatagcaata cattgaattt ccatggtcta gtcacgtatt
     3121 atcatttaat tgacaccaag tcgtgttatt gttgagctat cgagttcagc tcaaacattt
     3181 cttattccca tgaataagcc ggcaaaaata tgcaatctat gaaagttaat ataagcaaac
     3241 cttactttga ctcaatacca atgcactttg tgtcgatagg ttcacgcaat tgaggcgatt
     3301 attccgataa cccaagcgat tgactgttcc cgtttcgatt ccaattgaaa tttggaaatg
     3361 tacaatagtt ttgctatatg ctgtcaagta cgctcttatc ttctctgggt tttcttcaga
     3421 gtttcgaaac gcttcttctt ttttttgttt tttttttttt ggaatctcgt attttggaag
     3481 gggctcccct ctggaatttg ttacactgtc gttatcattg cgaacaagcg gcccgaagct
     3541 atcagcgact ttaacattta caatgcactt ttttacgacc aattaaatgt acattttcct
     3601 ttcttcgccc gttgataagc gaacgcgatg tggcgcaggc aatgtgttgc tcttgcgaca
     3661 caaacgcaat caaaatggat tcaatttcgc tttttcccag tgaaacgaag aacgaaccga
     3721 ccatcatgat atgctcctct gcatgttgcg tattgaatca atgacaattt caattaagcc
     3781 gcccgttcgt catgcgtttt cgtgcgcttc gaaatgctga taacgctgct gtcctccaac
     3841 tgctttgcat gtggacacaa ttccatttat ttaattcttt tatttggatc ggttaaatta
     3901 aaaagcgcct tgttacgcat ttaacgttgt ttccggtgcg tggtggtttc atgcttctgg
     3961 gaacggcaaa tgggtttagg attgggaacc cctcatcatc tgttggaata tactattcaa
     4021 cctacaaaag taacgttaaa caacactact ttatatttga tatgaatggc cacacctttt
     4081 atgccataaa acatattgta agagaatacc actcttttta ttccttcttt ccttcttgta
     4141 cgttttttgc tgtaagtagg tcgtggtgct ggtgttgcag ttgaaataac ttaaaatata
     4201 aatcataaaa ctcaaacata aacttgacta tttatttatt tattaagaaa ggaaatataa
     4261 attataaatt acaacaggtt atggacctgc agccaagctt ggcgctcgtc cgggggcaat
     4321 gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     4381 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     4441 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     4501 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     4561 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     4621 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     4681 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     4741 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     4801 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     4861 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     4921 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     4981 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     5041 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     5101 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     5161 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     5221 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     5281 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     5341 caaaggaata gagtagatgc cgaccgaaca agagctgatt tcgagaacgc ctcagccagc
     5401 aactcgcgcg agcctagcaa ggcaaatgcg agagaacggc cttacgcttg gtggcacagt
     5461 tctcgtccac agttcgctaa gctcgctcgg ctgggtcgcg ggagggccgg tcgcagtgat
     5521 tcaggccctt ctggattgtg ttggtcccca gggcacgatt gtcatgccca cgcactcggg
     5581 tgatctgact gatcccgcag attggagatc gccgcccgtg cctgccgatt gggtgcagat
     5641 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     5701 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     5761 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     5821 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     5881 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     5941 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
     6001 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
     6061 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
     6121 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
     6181 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
     6241 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     6301 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     6361 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6421 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6481 tctggatcaa ttcgttgcag gacaggatgt ggtgcccgat gtgactagct ctttgctgca
     6541 ggccgtccta tcctctggtt ccgataagag acccagaact ccggcccccc accgcccacc
     6601 gccaccccca tacatatgtg gtacgcaagt aagagtgcct gcgcatgccc catgtgcccc
     6661 accaagagct ttgcatccca tacaagtccc caaagtggag aaccgaacca attcttcgcg
     6721 ggcagaacaa aagcttctgc acacgtctcc actcgaattt ggagccggcc ggcgtgtgca
     6781 aaagaggtga atcgaacgaa agacccgtgt gtaaagccgc gtttccaaaa tgtataaaac
     6841 cgagagcatc tggccaatgt gcatcagttg tggtcagcag caaaatcaag tgaatcatct
     6901 cagtgcaact aaagggggga tctcgaggga tccgatatca acaagtttgt acaaaaaagc
     6961 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     7021 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
     7081 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     7141 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     7201 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     7261 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     7321 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     7381 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     7441 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     7501 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     7561 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     7621 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     7681 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     7741 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     7801 cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc
     7861 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     7921 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     7981 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     8041 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     8101 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     8161 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     8221 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     8281 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     8341 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     8401 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     8461 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     8521 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     8581 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     8641 gtggttgata tcggtggttc aagcggtggc agcggcggta aagacaatac tgtgcctctg
     8701 aagctgatcg ctctcctggc taatggcgag ttccatagtg gcgaacagct gggagaaacc
     8761 ctgggcatgt ccagggccgc tatcaacaag cacattcaga ctctgcgcga ctggggcgtg
     8821 gacgtgttca ccgtgcccgg aaagggctac tctctgcccg agcctatccc gctgctgaac
     8881 gctaaacaga ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac
     8941 tccaccaatc agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt
     9001 gcagaatatc agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga
     9061 gctaacctgt acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg
     9121 ggcccggtca tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg
     9181 cgagtcaaat ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg
     9241 gagctggccg gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg
     9301 gctatgaggc gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca
     9361 gggattaacc tggacaggaa tactctggcc gctacgctga tccgagagct gcgggcagcc
     9421 ctggaactgt tcgagcagga aggcctggct ccatatctgc cacggtggga gaagctggat
     9481 aacttcatca atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc
     9541 cgggggattg ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg
     9601 atgggcggag aaatcagtct gcggtctgcc gaaaaggcta gcggcaagcc catccccaac
     9661 cccctgctgg gcctggacag cacctaatga taatagtcta gtcgaccatg aagatcaaga
     9721 tcattgcccc gccagagcgc aagtactctg tctggatcgg tggctccatc ctggcttcgc
     9781 tgtccacctt ccagcagatg tggatctcca agcaggagta cgacgagtcc ggcccctcca
     9841 ttgtgcaccg caagtgcttc taagaaggat cgcttgtctg ggcaagagga tcaggatcgg
     9901 gatggtcttg attctgctgg aggaggagga ggagaagtcg aggaagcagc agcgaaagtg
     9961 caagtgcgag tggtggaagt ttggagtgca gcacaacaaa atcaacaaca acaccaacta
    10021 caagatgaaa agagcggaac cacctgcaca ccatcatcac tatcatcatc gttttgggcg
    10081 catgttgtgt ggttccagcg tattaatata attaatttat tccacatgag atatgatatg
    10141 atatactatg tattttttgt ttttttttta tttgtaaacc tttaatataa caagaactac
    10201 aaaaaatgaa aatgagcgaa aatgcatatt ctgccattcc acacacacac caacaacacc
    10261 caacacacgc acacccacaa gcttacacac acacattcgc ggcatgacaa ggacatcaag
    10321 ataaagaaga acttaaagaa gatatttccc aaagcgcaaa aagaacacac acacattgca
    10381 aaacacaaac aacacactag cgttttgtac aattcgtcag caaccttatg tattattttt
    10441 taattatgat gtaattataa acaaagtgaa acaaaaatat gaaaacaaaa aggaaaatca
    10501 aatctgtctt ctctttctcc cgctctcctc gctctctgct gctaacctcg ccctctcctc
    10561 tctcatcttt ttgtctgtct ctcttccaca tttttgccgg ccggcaaaat aataacccac
    10621 acacactcac acttggctgc agtttcgcgt gcgatattca cacacattca agcatacaag
    10681 catacataca tatgtatttt ttttttattt gtacactttt ctaattgcat gcgtatcgat
    10741 tgataagttt acgcctgaaa atgttaatta aaatgtgaaa atgcaactga aaaactgatg
    10801 aaatgaaaca acaacaagcg aacaatttgc tacatgtgta ttgtctaaca accgttactg
    10861 caacggttgc ttcggaaaag ggtgaagagg aagagggcac gaggtcgact agagcggccg
    10921 ccgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
    10981 atg
//
