LOCUS       Exported               12764 bp ds-DNA     circular SYN 05-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pM-Cas12a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12764)
  AUTHORS   Administrator
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 9, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12764
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..767
                     /label=2x35S
                     /note="2x35S"
     CDS             852..4739
                     /codon_start=1
                     /label=3xFlag-Cas12a
                     /translation="MDYKDDDDKMDYKDDDDKMDYKDDDDKPGGSSRVDMAPKKKRKVG
                     IHGVPAASKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDYKGVKKL
                     LDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEIAKAFKGN
                     EGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSEEAKSTSI
                     AFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGEFFNFVLT
                     QEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLSDRESLSF
                     YGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNGPAISTIS
                     KDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQLQEYADAD
                     LSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDLLDSVKSF
                     ENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSKDKFKLYF
                     QNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGNYEKINYK
                     LLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLIDFFKDSI
                     SRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVEEGKLYMF
                     QIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKEELVVHPA
                     NSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKINTEVRVLL
                     KHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDYHSLLDKK
                     EKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGFKNSRVKV
                     EKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQNGFIFYIP
                     AWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYKNFSRTD
                     ADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQGDIRALL
                     CEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNYEAQENAI
                     LPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSVKHKRPAA
                     TKKAGQAKKKK"
     CDS             852..932
                     /codon_start=1
                     /label=3xFlag
                     /note="3xFlag"
                     /translation="MDYKDDDDKMDYKDDDDKMDYKDDDDK"
     CDS             957..1007
                     /codon_start=1
                     /label=N-terminal NLS
                     /note="N-terminal NLS"
                     /translation="MAPKKKRKVGIHGVPAA"
     CDS             1008..4688
                     /codon_start=1
                     /label=LbCas12a
                     /note="Cas12a"
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     misc_feature    4689..4739
                     /label=Nucleoplasmin NLS
                     /note="Nucleoplasmin NLS"
     CDS             complement(5018..5926)
                     /codon_start=1
                     /label=NSm
                     /note="NSm"
                     /translation="MLTFFGNKGSSKSARKDEGPLVSLAKHNGNVEVSKPWSSSDEKLA
                     LTKAMDTSKGKILLNTEGTSSFGTYESDSITESEGYDLSARMIVDTNHHISNWKNDLFV
                     GNGKQNANKVIKICPTWDSRKQYMMISRIVIWVCPTIPNPTGKLVVALVDPNMPSEKQI
                     ILKGQGTITDPICFVFYLNWSIPKMNNTPENCCQLHLMCSQEYKKGVSFGSVMYSWTKE
                     FCDSPRADKDKSCMVIPLNRAIRARSQAFIEACKLIIPKGNSEKQIKKQLKELSSNLER
                     SVEEEEEGVSDNVAQLSFDEI"
     terminator      6125..6377
                     /label=Nos
                     /note="NOS T"
     misc_feature    6425..6449
                     /label=RB
                     /note="RB"
     rep_origin      6577..7919
                     /label=2u ori
                     /note="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     promoter        8178..8459
                     /gene="S. cerevisiae TRP1"
                     /label=S. cerevisiae TRP1 promoter
                     /note="TRP1 promoter"
     CDS             8460..9134
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required
                     for tryptophan biosynthesis"
                     /label=S. cerevisiae TRP1
                     /note="TRP1"
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     CDS             complement(9515..10663)
                     /codon_start=1
                     /label=trfA
                     /note="trfA"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10962..11756)
                     /codon_start=1
                     /label=KanR
                     /note="KanR"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11968..12599
                     /label=oriV
                     /note="oriV"
     misc_feature    12677..12701
                     /label=LB
                     /note="LB"
ORIGIN
        1 aagcttgcat gcctgcagtc aacatggtgg agcacgacac tctcgtctac tccaagaata
       61 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
      121 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      181 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaaa
      241 gaatgcctct accgacagtg gtcccaaaga tggacccccc acccacgagg aacatcgtgg
      301 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg
      361 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg
      421 ctattgagac tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
      481 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
      541 ttgcgataaa ggaaaggcta tcgttcaaga atgcctctac cgacagtggt cccaaagatg
      601 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      661 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
      721 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaga gcaatcagtg
      781 caaacaaaaa ccttaatcca gacatcttga aattaatcat ataaccatta taatctgagc
      841 agacgtataa gatggattac aaggatgacg acgataagat ggattataaa gacgatgacg
      901 ataagatgga ttacaaggat gacgacgata agcccggggg atcctctaga gtcgacatgg
      961 ctcctaagaa gaagcggaag gttggtattc acggggtgcc tgcggcttca aagctcgaga
     1021 aattcaccaa ctgttattcg ttgagcaaaa cactgcggtt taaagcgatt ccagtcggca
     1081 agactcaaga gaatatagac aataagcggc tgttggtgga agatgaaaag cgcgcggaag
     1141 actacaaagg ggtgaagaag ttgttggaca gatactacct ctcttttatc aatgatgtct
     1201 tgcactcaat caaattgaag aatctgaaca actacatctc cctcttcaga aagaaaacaa
     1261 ggacagaaaa ggagaataag gaacttgaaa atttggagat caatctgagg aaagagatcg
     1321 cgaaagcctt taaaggcaac gaaggataca aaagtctgtt caagaaggat ataattgaga
     1381 caattttgcc agagttcctc gatgacaagg acgagattgc gctggtcaat tcgttcaacg
     1441 gattcacaac agcattcaca ggcttctttg ataatcggga aaatatgttc tctgaggagg
     1501 caaagtccac ttctattgcg ttcaggtgta tcaatgagaa tctcactagg tacatttcca
     1561 acatggatat ctttgagaag gttgacgcaa tttttgacaa gcacgaagtt caggagatta
     1621 aggagaagat cctcaattcc gattatgacg ttgaggactt cttcgaaggt gagtttttta
     1681 atttcgtgct cactcaagag ggtatcgacg tgtataatgc gatcatcggt gggttcgtga
     1741 ctgagtccgg tgaaaagatt aagggattga acgagtatat caacctttac aaccaaaaga
     1801 cgaaacagaa gctgccaaag ttcaagcctc tttacaaaca ggttctttca gaccgcgagt
     1861 cactctcgtt ctatggggag ggctacactt cggatgagga agtcctggag gtgttcagga
     1921 atactctcaa taagaattcg gagattttct cttctataaa aaaactggaa aagttgttta
     1981 agaattttga cgaatactct agcgccggca tatttgtgaa aaacggcccg gccatatcaa
     2041 cgataagtaa agatatcttc ggcgaatgga acgtgatcag agacaaatgg aacgcggagt
     2101 atgacgatat tcacctgaag aagaaggctg tcgtaacgga gaagtacgag gatgatcgca
     2161 ggaaaagctt caaaaagatc ggaagtttca gcctggaaca gttgcaggag tatgctgacg
     2221 ccgatcttag cgtcgtcgag aagttgaagg agataatcat ccaaaaggtc gacgagatat
     2281 ataaagtcta tggatcaagt gaaaaactgt tcgacgccga cttcgttttg gagaagtccc
     2341 tgaagaagaa cgacgctgtt gttgccatta tgaaggatct gctcgacagc gtgaagagtt
     2401 tcgagaacta tattaaggct tttttcgggg aggggaagga gactaacaga gatgagtcct
     2461 tctacggaga cttcgtcctc gcgtacgata tactccttaa ggtagaccac atctacgacg
     2521 caatcagaaa ttacgtgaca caaaagccgt acagcaagga caagttcaaa ctctacttcc
     2581 agaaccccca gttcatgggc ggctgggaca aggacaagga aacggattac agggctacga
     2641 tcctgaggta tggttcaaaa tactacttgg cgattatgga caagaagtac gccaagtgtc
     2701 tccagaagat tgacaaagac gatgtcaatg gcaattatga gaagatcaac tacaagctgc
     2761 ttccgggtcc gaacaagatg ctcccaaagg ttttcttcag caagaaatgg atggcctact
     2821 ataacccaag cgaggacatc cagaagattt ataagaacgg tacgttcaag aagggcgaca
     2881 tgttcaatct taacgactgt cacaagctga tcgacttctt caaagactca attagccggt
     2941 acccaaagtg gtctaacgcc tatgacttca acttttcgga aaccgagaag tacaaggata
     3001 tagccggatt ttatagagag gtggaagagc agggctacaa ggtgtcattc gagtccgcca
     3061 gcaagaagga agtggacaag ctcgtggaag agggtaagct ctacatgttc cagatttata
     3121 ataaagactt tagcgataag agccacggga cacctaatct ccacacaatg tatttcaagc
     3181 tgctcttcga cgagaataac cacggccaaa tcaggttgtc aggaggggct gaactcttca
     3241 tgcggcgcgc tagccttaag aaggaggagc ttgtagtcca ccctgcgaat agtccaattg
     3301 cgaataagaa cccggacaat cctaaaaaga ctacaacatt gagctacgac gtgtacaagg
     3361 ataagaggtt ttccgaggat cagtacgagc tccacatccc gattgcgatc aacaagtgcc
     3421 caaagaatat tttcaagata aacacagagg tgcgtgtact cctgaagcat gacgacaatc
     3481 cttacgtcat tgggattgat cggggcgaga ggaacctcct ctatattgtg gtggtggacg
     3541 ggaaggggaa catagtcgaa cagtactccc ttaacgaaat aattaacaat ttcaacggca
     3601 tccgtatcaa gaccgactac cattcgttgc tggacaagaa ggagaaggag agatttgagg
     3661 cgcggcaaaa ttggacaagt atcgagaaca tcaaggaact caaagcaggt tatatctctc
     3721 aagttgtgca taagatatgc gagctggttg agaagtatga cgcagtgatc gctcttgagg
     3781 acctcaactc gggctttaag aattctagag ttaaagtgga gaagcaggtc tatcaaaagt
     3841 tcgagaagat gcttatagat aagctcaact acatggtcga taagaaatcg aacccatgtg
     3901 ccaccggcgg cgcactcaaa ggttaccaaa taacaaacaa attcgagtcc ttcaaatcga
     3961 tgagtactca gaatgggttc atattttata taccggcgtg gcttacgtct aagatcgacc
     4021 cgtcaactgg ttttgtcaac ctgttgaaga cgaaatacac gtccattgcc gattcgaaaa
     4081 agttcatatc tagttttgat cgtattatgt acgtcccaga ggaagatctt ttcgagtttg
     4141 ctctcgacta caaaaacttt tcgcggaccg atgcggatta cattaaaaaa tggaaactct
     4201 attcgtacgg caacagaatc aggatttttc gcaaccctaa gaagaataac gtctttgatt
     4261 gggaggaagt ttgcttgact agcgcgtaca aggagctctt taataagtat ggcattaact
     4321 accaacaggg tgatatcaga gcactgcttt gcgaacaatc tgacaaggct ttctactcat
     4381 ccttcatggc tttgatgagc ctgatgctcc agatgagaaa ttcaattaca ggcagaaccg
     4441 acgtggattt cttgatctcc ccggttaaaa attctgatgg catcttttac gatagcagga
     4501 actatgaagc gcaagagaat gcgattctgc caaaaaatgc agacgccaac ggtgcctata
     4561 acatcgccag gaaagtcctg tgggcgatcg gccagttcaa aaaggccgaa gacgaaaaat
     4621 tggacaaggt caaaatcgct atcagcaaca aagagtggct ggagtatgct cagacatccg
     4681 taaagcataa gcgtcctgct gccaccaaaa aggccggaca ggctaagaaa aagaagtgac
     4741 gcgtcgacgg ctgcttgtca caagctaaat ttgatcctat ttcctacatt aatcgaatta
     4801 tatatatata aaataaacaa aaaacaaaca aaaatacaaa taaacaaaaa acaacaaaaa
     4861 gagtcttcgg accaaagttt ggcttttcag ccttattttg tttttgtttt ttgtttttga
     4921 ttttttgttt gttttttatt tttgtttttg tttggttttt gatttaaaaa tgcaaaataa
     4981 acagagattt aagcttaaat aagtggtatt aaaagtacta tatttcatca aaagataact
     5041 gagcaacatt atcagaaacc ccttcctcct cttcttcaac tgatctctca agatttgagc
     5101 tcagttcttt aagctgtttt ttaatctgct tctcactgtt tcctttagga attatcagct
     5161 tgcaagcctc aatgaatgct tgagatctag ctctaatagc cctgtttaga ggtatgacca
     5221 tgcaactttt gtctttatca gctctgggtg aatcacaaaa ctcctttgtc caagaataca
     5281 tgacactacc aaaagaaacc cccttcttgt attcttgact gcacatcaag tgcagctgac
     5341 agcagttttc tggagtgtta ttcattttcg gaatagacca gttcagataa aaaacaaaac
     5401 agataggatc agttattgtc ccctgaccct tcagaatgat ttgcttttca gatggcatgt
     5461 tgggatcgac cagagcaacc acaagtttcc ctgtagggtt tggtatagtg gggcagaccc
     5521 atatcacaat cctggaaatc atcatgtatt gttttctgct gtcccaagtt ggacagatct
     5581 tgatgacctt attagcgttt tgcttcccgt tgccgacaaa aagatcattt ttccagtttg
     5641 agatatggtg gtttgtatct actatcattc ttgcagaaag atcataaccc tctgattctg
     5701 tgatagaatc agattcatat gttccaaagg aagatgttcc ctctgtgttc aacagtatct
     5761 ttcctttgga tgtatccata gctttggtta aagcaagctt ttcatcagaa gaagaccatg
     5821 gttttgagac ttcaacatta ccgttatgtt tagcaagtga aactaaaggc ccttcatcct
     5881 ttctggcaga cttagaagac cccttattac caaaaaaagt caacattttg agttcaacag
     5941 cctgaggtag aggagcttgt gtaatgtaat tgattgattc ttagcaaagt gtataatagg
     6001 tatatttctg atgcactgat tgctctgggt cggcatggca tctccacctc ctcgcggtcc
     6061 gacctgggca tccgaaggag gacgtcgtcc actcggatgg ctaagggaga gctcgaattt
     6121 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     6181 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     6241 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     6301 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     6361 atctatgtta ctagatcgga attcagattg tcgtttcccg ccttcagttt aaactatcag
     6421 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     6481 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     6541 cacaggagat ctcagtaaag cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
     6601 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     6661 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     6721 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     6781 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     6841 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     6901 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     6961 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     7021 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     7081 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     7141 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
     7201 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
     7261 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
     7321 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
     7381 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
     7441 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
     7501 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
     7561 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
     7621 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
     7681 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
     7741 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
     7801 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
     7861 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
     7921 tattaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     7981 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8041 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     8101 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     8161 agagtgcacc atagatcaac gacattacta tatatataat ataggaagca tttaatagaa
     8221 cagcatcgta atatatgtgt actttgcagt tatgacgcca gatggcagta gtggaagata
     8281 ttctttattg aaaaatagct tgtcacctta cgtacaatct tgatccggag cttttctttt
     8341 tttgccgatt aagaattaat tcggtcgaaa aaagaaaagg agagggccaa gagggagggc
     8401 attggtgact attgagcacg tgagtatacg tgattaagca cacaaaggca gcttggagta
     8461 tgtctgttat taatttcaca ggtagttctg gtccattggt gaaagtttgc ggcttgcaga
     8521 gcacagaggc cgcagaatgt gctctagatt ccgatgctga cttgctgggt attatatgtg
     8581 tgcccaatag aaagagaaca attgacccgg ttattgcaag gaaaatttca agtcttgtaa
     8641 aagcatataa aaatagttca ggcactccga aatacttggt tggcgtgttt cgtaatcaac
     8701 ctaaggagga tgttttggct ctggtcaatg attacggcat tgatatcgtc caactgcatg
     8761 gagatgagtc gtggcaagaa taccaagagt tcctcggttt gccagttatt aaaagactcg
     8821 tatttccaaa agactgcaac atactactca gtgcagcttc acagaaacct cattcgttta
     8881 ttcccttgtt tgattcagaa gcaggtggga caggtgaact tttggattgg aactcgattt
     8941 ctgactgggt tggaaggcaa gagagccccg aaagcttaca ttttatgtta gctggtggac
     9001 tgacgccaga aaatgttggt gatgcgctta gattaaatgg cgttattggt gttgatgtaa
     9061 gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa
     9121 atgctaagaa ataggttatt actgagtagt atttatttaa gtattgtttg tgcacttgcc
     9181 gatctatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     9241 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     9301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     9361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     9421 caaagggcga aaaaccgtct cggtgcgggc ctcttcgcta ttacgccaga tccttgctgg
     9481 ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat
     9541 cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga
     9601 cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt
     9661 ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg
     9721 tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca
     9781 gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca
     9841 ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga
     9901 agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc
     9961 cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc
    10021 cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg
    10081 cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct
    10141 tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg
    10201 ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa
    10261 gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc
    10321 tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc
    10381 gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac
    10441 gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct
    10501 cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg
    10561 cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga
    10621 tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga
    10681 ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct
    10741 tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt
    10801 ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca
    10861 aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc
    10921 gctcctgctt gtcgccggca tcgttgcgcc acatctaggt actaaaacaa ttcatccagt
    10981 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
    11041 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
    11101 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
    11161 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
    11221 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
    11281 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
    11341 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
    11401 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
    11461 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
    11521 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
    11581 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
    11641 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
    11701 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
    11761 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
    11821 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
    11881 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac
    11941 aattatgggt gatgctgcca actcgagagc gggccgggag ggttcgagaa gggggggcac
    12001 cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta
    12061 taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga
    12121 aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg
    12181 cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag
    12241 tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca
    12301 tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc
    12361 acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg
    12421 cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt
    12481 atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt
    12541 ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtct
    12601 agtggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    12661 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    12721 ttaataacac attgcggacg tttttaatgt actggggtgg tttt
//
