LOCUS       Exported               13802 bp ds-DNA     circular SYN 05-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pM-ABE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13802)
  AUTHORS   Administrator
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 9, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13802
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..767
                     /label=2x35S
                     /note="2x35S"
     CDS             858..5771
                     /codon_start=1
                     /label=ABE
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVSEVEFSHEYWMRH
                     ALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNY
                     RLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGWRNSKRGAAGSLMNVLNYPGMNHRVEI
                     TEGILADECAALLCDFYRMPRQVFNAQKKAQSSINSGGSSGGSSGSETPGTSESATPES
                     SGGSSGGSIHGVPAADKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDSGGSPKKKRKVSGGSPKKKRKVSGGSPKKKRKV"
     CDS             861..926
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="3xFLAG"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             933..953
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             954..1451
                     /codon_start=1
                     /label=TadA8e V106W
                     /note="TadA8e V106W"
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     WRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQS
                     SIN"
     misc_feature    1452..1547
                     /label=linker
                     /note="linker"
     CDS             1569..5668
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /label=nCas9
                     /note="Cas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGG"
     misc_feature    1593..1595
                     /label=D10A
                     /note="D10A"
     CDS             5682..5768
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=3xSV40 NLS
                     /note="SV40 NLS"
                     /translation="PKKKRKVSGGSPKKKRKVSGGSPKKKRKV"
     CDS             complement(6056..6964)
                     /codon_start=1
                     /label=NSm
                     /note="NSm"
                     /translation="MLTFFGNKGSSKSARKDEGPLVSLAKHNGNVEVSKPWSSSDEKLA
                     LTKAMDTSKGKILLNTEGTSSFGTYESDSITESEGYDLSARMIVDTNHHISNWKNDLFV
                     GNGKQNANKVIKICPTWDSRKQYMMISRIVIWVCPTIPNPTGKLVVALVDPNMPSEKQI
                     ILKGQGTITDPICFVFYLNWSIPKMNNTPENCCQLHLMCSQEYKKGVSFGSVMYSWTKE
                     FCDSPRADKDKSCMVIPLNRAIRARSQAFIEACKLIIPKGNSEKQIKKQLKELSSNLER
                     SVEEEEEGVSDNVAQLSFDEI"
     terminator      7154..7422
                     /label=Nos
                     /label=NOS terminator
                     /note="NOS terminator"
     misc_feature    7463..7487
                     /label=RB T-DNA
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7615..8957
                     /label=2u ori
                     /note="2u ori"
     protein_bind    complement(8578..8625)
                     /note="FRT"
     promoter        9216..9497
                     /label=S. cerevisiae TRP1 promoter
                     /note="TRP1 promoter"
     CDS             9498..10172
                     /codon_start=1
                     /label=TRP1
                     /note="TRP1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        10508..10513
                     /note="ADH1 promoter"
     CDS             complement(10553..11701)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to
                     and activates oriV"
                     /label=trfA
                     /note="trfA"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    complement(12000..12794)
                     /label=Km
                     /note="Km"
     CDS             complement(12000..12794)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA-3
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13006..13637
                     /label=oriV
                     /note="oriV"
                     /note="incP origin of replication"
     misc_feature    complement(13636..13792)
                     /label=LB
                     /note="LB"
     misc_feature    13715..13739
                     /label=LB T-DNA
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcagtc aacatggtgg agcacgacac tctcgtctac tccaagaata
       61 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
      121 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      181 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaaa
      241 gaatgcctct accgacagtg gtcccaaaga tggacccccc acccacgagg aacatcgtgg
      301 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg
      361 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg
      421 ctattgagac tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
      481 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
      541 ttgcgataaa ggaaaggcta tcgttcaaga atgcctctac cgacagtggt cccaaagatg
      601 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      661 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
      721 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaga gcaatcagtg
      781 caaacaaaaa ccttaatcca gacatcttga aattaatcat ataaccatta taatctgagc
      841 agacgtataa gactagtatg gattacaagg accacgacgg tgattataag gatcacgaca
      901 tcgactacaa ggacgatgat gacaagatgg ctccgaagaa gaaaaggaaa gtgtctgaag
      961 tagagttctc ccacgagtac tggatgaggc acgctcttac attggccaag cgtgctaggg
     1021 acgagagaga ggtgcctgtc ggagctgtgc ttgtactgaa caaccgagta atcggcgaag
     1081 gctggaaccg tgccattggt ctgcatgatc ccaccgcaca cgcagagatc atggcacttc
     1141 gtcaaggagg tcttgtaatg cagaactacc gtctgataga tgcaactctc tacgtgacct
     1201 tcgagccctg tgtcatgtgt gctggagcaa tgatccactc ccgtatcggt cgagtggtat
     1261 tcggctggcg taacagcaag agaggtgctg ctggtagcct catgaacgtg ctgaactacc
     1321 ctggtatgaa ccatcgtgta gagatcactg agggcattct tgcagacgag tgtgctgcac
     1381 tgctctgcga cttctacagg atgcctaggc aggtgttcaa tgctcagaag aaggctcaga
     1441 gctccatcaa tagcggtgga tcctcaggtg gatcttcagg cagtgagaca cctggaacca
     1501 gtgagtcagc tactccagag tcaagtggtg gatcttcagg aggctcaata cacggtgttc
     1561 cagcagccga taagaagtac agtatcggac ttgccatagg gactaactct gtcgggtggg
     1621 cagtaatcac agatgagtac aaagtcccat ctaagaagtt caaggtgctc ggtaacaccg
     1681 atagacacag catcaagaag aacctcatcg gtgctctcct gttcgattcc ggcgaaacgg
     1741 ccgaagccac taggttgaag aggacggccc gacgacgtta tacccgtaga aagaatagga
     1801 tatgctatct gcaggagata ttctccaacg agatggctaa ggtagacgat agcttcttcc
     1861 atcgtctcga ggagtccttc ctggtggaag aggacaagaa acatgaaaga cacccaatct
     1921 ttgggaacat tgttgacgag gtcgcatatc atgagaagta cccaacaatc taccacttga
     1981 ggaagaaact cgtcgacagt actgacaagg ctgatctgcg tttaatctac ttagcactgg
     2041 ctcatatgat caagttcaga ggtcacttct tgatcgaggg tgacttgaac cctgacaatt
     2101 cagacgtaga caagctgttc atccagttag tccagacgta taatcagttg ttcgaggaaa
     2161 acccaattaa cgctagtgga gtcgacgcca aggcaatatt gagtgcaaga ctctccaagt
     2221 cccgaaggtt ggaaaacctc attgctcaat tgcctggaga gaagaagaac gggctcttcg
     2281 gaaatcttat agctctgtcc ttagggttaa cgccgaattt caagtccaac ttcgacctcg
     2341 ccgaggacgc caagttgcaa ttgtccaagg atacctacga cgatgacctc gacaacttac
     2401 tggctcagat cggcgaccag tacgctgacc tcttcttggc cgctaagaac ttgtcagacg
     2461 caatcctctt aagtgatatc ttacgtgtga acacagagat tacgaaagct cctttgagcg
     2521 catccatgat caagagatac gatgagcacc atcaggattt gacgttgttg aaggccttag
     2581 ttcgtcagca attgcccgag aagtacaagg agatcttctt cgatcagtcc aagaacggat
     2641 acgcagggta catcgatggt ggtgctagtc aagaggagtt ttacaagttc atcaagccca
     2701 tactcgagaa gatggatgga actgaggaac tgttagtgaa gctgaatcgt gaggacctgt
     2761 taaggaagca gcgaacgttc gacaatggca gcatcccgca ccagatccac cttggcgagc
     2821 tgcatgcaat cttgaggaga caggaagact tctatccatt cttgaaggac aacagagaga
     2881 agatcgagaa gatcttgacg ttcagaatcc cgtattacgt tggtccactc gccaggggta
     2941 actcccgttt cgcttggatg acgaggaaat cagaggagac aatcactcct tggaacttcg
     3001 aggaggtggt tgacaaagga gccagcgctc aatccttcat agagcgtatg accaatttcg
     3061 acaagaatct cccaaacgag aaggtcctgc cgaaacactc attgttgtac gagtacttca
     3121 ctgtgtataa cgagctcacg aaggtcaaat acgtaactga gggtatgcga aagcccgcct
     3181 tcctctccgg tgaacagaag aaggcaatcg tcgatttgtt gttcaagacc aacaggaagg
     3241 tcactgtgaa gcaactgaaa gaggactact tcaagaagat agagtgcttc gattctgtgg
     3301 agatcagtgg tgttgaggat cgtttcaacg catccttggg cacataccat gatttgttga
     3361 agatcatcaa ggacaaggac ttcctcgaca acgaggagaa cgaggacatc ctcgaggata
     3421 tcgttctcac attgacgctc ttcgaggatc gtgagatgat cgaggagaga ctgaagacct
     3481 acgctcattt gttcgatgat aaggtgatga agcaactcaa gaggcgtcga tacacgggat
     3541 ggggaaggtt gtctcggaag ctgatcaacg gtattaggga taaacagtct ggcaagacaa
     3601 tcttggactt cttgaagagt gatggattcg caaataggaa tttcatgcag ttaatccacg
     3661 atgatagtct gacgttcaag gaggacatcc aaaaggccca agtgagtggt cagggagaca
     3721 gcctccatga acatatagca aacttggctg gatccccagc aattaagaaa ggaatcttgc
     3781 agacggtcaa agttgtggat gaacttgtta aggtcatggg tcgtcacaaa cctgagaata
     3841 tcgttatcga aatggcccga gagaatcaga cgacccagaa aggacagaag aactctcgtg
     3901 aacgtatgaa acgaatagaa gaagggatca aagagctcgg tagccagatc ctgaaggagc
     3961 acccagtaga gaacactcaa ctgcagaacg agaagctgta tctgtactac ttgcaaaacg
     4021 ggcgagatat gtatgttgac caggagctcg acatcaatag actttctgac tacgatgttg
     4081 accatattgt gccgcaaagc ttcttgaagg atgatagcat agataacaaa gttctcacac
     4141 gttccgacaa gaacagaggt aagagcgaca acgtgccgag tgaagaagtc gttaagaaga
     4201 tgaagaacta ctggaggcag ttgctcaacg ctaagttgat cacccagagg aagttcgaca
     4261 acctcacaaa ggctgagaga ggaggcctgt cagagttgga taaggctggg ttcatcaaga
     4321 ggcagcttgt cgagacacga cagatcacca agcacgtagc ccagatcctc gattcaagga
     4381 tgaacaccaa gtacgatgag aacgacaagc tcatcaggga agtgaaggta atcacgctga
     4441 agagcaagct cgtatccgac ttccgaaagg actttcagtt ctacaaggtg cgtgagatca
     4501 acaactacca ccatgctcat gatgcatatc ttaatgcagt ggttgggacg gctctcatta
     4561 agaagtaccc aaagctcgaa tctgaatttg tgtatggcga ttacaaggtt tatgatgtcc
     4621 gtaagatgat tgctaagagc gagcaggaga ttggtaaggc taccgcgaag tacttcttct
     4681 actctaacat catgaacttc ttcaagacgg agatcaccct ggccaacgga gagatcagga
     4741 agcgtccgct catcgagacc aacggtgaga ccggtgagat cgtgtgggac aagggacgtg
     4801 acttcgccac tgtgaggaag gtgttgagca tgccgcaggt gaacatcgtg aagaagacgg
     4861 aggtgcagac cggagggttc tccaaggaat caatcttgcc aaagaggaac tccgataaac
     4921 tcatagcaag gaagaaggat tgggatccca agaagtatgg agggttcgac tccccgaccg
     4981 tcgcctacag cgtcttggtt gtggcaaagg tggagaaggg aaagagcaag aagttgaaat
     5041 cagtcaagga gctccttgga atcacaatca tggagcgaag ttctttcgag aagaacccca
     5101 tagacttctt ggaggccaaa ggttacaaag aagtgaagaa agacttgata atcaagttgc
     5161 ctaagtactc cttgttcgag ctcgagaacg ggaggaagcg aatgttggcc agcgccggag
     5221 aacttcagaa aggcaacgag ctcgctctcc caagcaagta cgtgaatttc ttgtacctcg
     5281 catcacacta cgagaagctg aagggcagcc cggaggataa cgaacagaag cagctcttcg
     5341 tggagcaaca caagcactac ttggatgaga tcatcgagca gattagcgag ttcagcaaga
     5401 gggtaatctt ggctgacgct aatctggaca aggttctcag cgcatacaac aagcatcgtg
     5461 acaaacccat ccgagagcag gcagagaaca tcatccactt gttcaccctg actaatttgg
     5521 gagcacccgc agccttcaag tacttcgata ctaccatcga ccggaagaga tacacaagta
     5581 ccaaggaggt cctggatgca accttgatcc atcagtccat caccggcttg tacgagacta
     5641 ggatcgacct ctctcagttg ggtggagact ccggcggcag cccaaagaag aagcggaagg
     5701 tgtctggagg ttctcctaag aaaaagagaa aagtgtccgg cggctccccg aagaagaagc
     5761 gcaaggtgta ggagctacgc gtcgacggct gcttgtcaca agctaaattt gatcctattt
     5821 cctacattaa tcgaattata tatatataaa ataaacaaaa aacaaacaaa aatacaaata
     5881 aacaaaaaac aacaaaaaga gtcttcggac caaagtttgg cttttcagcc ttattttgtt
     5941 tttgtttttt gtttttgatt ttttgtttgt tttttatttt tgtttttgtt tggtttttga
     6001 tttaaaaatg caaaataaac agagatttaa gcttaaataa gtggtattaa aagtactata
     6061 tttcatcaaa agataactga gcaacattat cagaaacccc ttcctcctct tcttcaactg
     6121 atctctcaag atttgagctc agttctttaa gctgtttttt aatctgcttc tcactgtttc
     6181 ctttaggaat tatcagcttg caagcctcaa tgaatgcttg agatctagct ctaatagccc
     6241 tgtttagagg tatgaccatg caacttttgt ctttatcagc tctgggtgaa tcacaaaact
     6301 cctttgtcca agaatacatg acactaccaa aagaaacccc cttcttgtat tcttgactgc
     6361 acatcaagtg cagctgacag cagttttctg gagtgttatt cattttcgga atagaccagt
     6421 tcagataaaa aacaaaacag ataggatcag ttattgtccc ctgacccttc agaatgattt
     6481 gcttttcaga tggcatgttg ggatcgacca gagcaaccac aagtttccct gtagggtttg
     6541 gtatagtggg gcagacccat atcacaatcc tggaaatcat catgtattgt tttctgctgt
     6601 cccaagttgg acagatcttg atgaccttat tagcgttttg cttcccgttg ccgacaaaaa
     6661 gatcattttt ccagtttgag atatggtggt ttgtatctac tatcattctt gcagaaagat
     6721 cataaccctc tgattctgtg atagaatcag attcatatgt tccaaaggaa gatgttccct
     6781 ctgtgttcaa cagtatcttt cctttggatg tatccatagc tttggttaaa gcaagctttt
     6841 catcagaaga agaccatggt tttgagactt caacattacc gttatgttta gcaagtgaaa
     6901 ctaaaggccc ttcatccttt ctggcagact tagaagaccc cttattacca aaaaaagtca
     6961 acattttgag ttcaacagcc tgaggtagag gagcttgtgt aatgtaattg attgattctt
     7021 agcaaagtgt ataataggta tatttctgat gcactgattg ctctgggtcg gcatggcatc
     7081 tccacctcct cgcggtccga cctgggcatc cgaaggagga cgtcgtccac tcggatggct
     7141 aagggagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     7201 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     7261 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     7321 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     7381 attatcgcgc gcggtgtcat ctatgttact agatcggaat tcagattgtc gtttcccgcc
     7441 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     7501 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     7561 ttgtatgtgc atgccaacca caggagatct cagtaaagcg aaaagtgcca cctgaacgaa
     7621 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     7681 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
     7741 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
     7801 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
     7861 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
     7921 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
     7981 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
     8041 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
     8101 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
     8161 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
     8221 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
     8281 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
     8341 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
     8401 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
     8461 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
     8521 gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa
     8581 gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa
     8641 acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca
     8701 cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt
     8761 tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc
     8821 tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt
     8881 agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt
     8941 tcctttgata ttggatcata ttaagaaacc attattatca tgacattaac ctataaaaat
     9001 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     9061 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     9121 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     9181 tcagagcaga ttgtactgag agtgcaccat agatcaacga cattactata tatataatat
     9241 aggaagcatt taatagaaca gcatcgtaat atatgtgtac tttgcagtta tgacgccaga
     9301 tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg tacaatcttg
     9361 atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag
     9421 agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca
     9481 caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga
     9541 aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact
     9601 tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga
     9661 aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg
     9721 gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg
     9781 atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc
     9841 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac
     9901 agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt
     9961 tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt
    10021 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg
    10081 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa
    10141 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt
    10201 attgtttgtg cacttgccga tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10261 aataccgcat caggaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg
    10321 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
    10381 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
    10441 gaacgtggac tccaacgtca aagggcgaaa aaccgtctcg gtgcgggcct cttcgctatt
    10501 acgccagatc cttgctggct gaacccccag ccggaactga ccccacaagg ccctagcgtt
    10561 tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg cctcgcaact
    10621 cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc
    10681 acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt
    10741 cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg aatttcgtgt
    10801 agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg caacgggacg
    10861 ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc
    10921 caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg
    10981 ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg
    11041 tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc tgctgccaca
    11101 ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc acgtccttgt
    11161 tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg
    11221 tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg
    11281 caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg
    11341 cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt tttcgcttct
    11401 tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct
    11461 gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca gggggagcca
    11521 gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag ccgacggact
    11581 ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg
    11641 cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac gtccgattca
    11701 ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct tattcctgat
    11761 ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg aagtcggtcc
    11821 cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc tgcacgaata
    11881 ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc ctgagagcca aaacacttga
    11941 tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac atctaggtac
    12001 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
    12061 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
    12121 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
    12181 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
    12241 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
    12301 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
    12361 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
    12421 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
    12481 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
    12541 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
    12601 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
    12661 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
    12721 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
    12781 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
    12841 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
    12901 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
    12961 agccgatttt gaaaccacaa ttatgggtga tgctgccaac tcgagagcgg gccgggaggg
    13021 ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct
    13081 ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt
    13141 ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct
    13201 caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc
    13261 gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc
    13321 ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt
    13381 gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg
    13441 ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg
    13501 ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg
    13561 acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa
    13621 ccagcccggt gagcgtctag tggactgatg ggctgcctgt atcgagtggt gattttgtgc
    13681 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
    13741 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggtt
    13801 tt
//
