LOCUS       Exported               14057 bp ds-DNA     circular SYN 05-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pM-CBE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14057)
  AUTHORS   Administrator
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 9, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14057
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..767
                     /label=2x35S
                     /note="2x35S"
     CDS             858..6032
                     /codon_start=1
                     /label=CBE
                     /note="CBE"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIREASPASGPRH
                     LMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSVKMDQHRGFLHNQAKNLLCGFYGRH
                     AELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWGCAGEVRAFLQENTHVRLRIFAARI
                     YDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDTFVDHQGCPFQPWDGLDEHSQALSG
                     RLRAILQNQGNSGSETPGTSESATPESIHGVPAADKKYSIGLAIGTNSVGWAVITDEYK
                     VPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEI
                     FSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVD
                     STDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINA
                     SGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDA
                     KLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASM
                     IKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPIL
                     EKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREK
                     IEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNF
                     DKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
                     KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDIL
                     EDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQS
                     GKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIK
                     KGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGS
                     QILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSI
                     DNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSEL
                     DKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDF
                     QFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEI
                     GKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVA
                     KVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFEL
                     ENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHY
                     LDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAF
                     KYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDTRDSGGSTNLSDIIE
                     KETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPW
                     ALVIQDSNGENKIKMLSGGSPKKKRKV"
     CDS             861..926
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="3xFLAG"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             933..953
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             963..1555
                     /codon_start=1
                     /label=A3A
                     /note="A3A"
                     /translation="EASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSV
                     KMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWG
                     CAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDT
                     FVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQG"
     CDS             1557..1604
                     /codon_start=1
                     /label=XTEN linker
                     /note="XTEN linker"
                     /translation="SGSETPGTSESATPES"
     CDS             1626..5725
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /label=nCas9
                     /note="Cas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGG"
     misc_feature    1650..1652
                     /label=D10A
                     /note="D10A"
     misc_feature    5736..5747
                     /label=SGGS
                     /note="SGGS"
     CDS             5748..5995
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /note="UGI"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKM"
     misc_feature    5997..6008
                     /label=SGGS
     CDS             6009..6029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             complement(6311..7219)
                     /codon_start=1
                     /label=NSm
                     /note="NSm"
                     /translation="MLTFFGNKGSSKSARKDEGPLVSLAKHNGNVEVSKPWSSSDEKLA
                     LTKAMDTSKGKILLNTEGTSSFGTYESDSITESEGYDLSARMIVDTNHHISNWKNDLFV
                     GNGKQNANKVIKICPTWDSRKQYMMISRIVIWVCPTIPNPTGKLVVALVDPNMPSEKQI
                     ILKGQGTITDPICFVFYLNWSIPKMNNTPENCCQLHLMCSQEYKKGVSFGSVMYSWTKE
                     FCDSPRADKDKSCMVIPLNRAIRARSQAFIEACKLIIPKGNSEKQIKKQLKELSSNLER
                     SVEEEEEGVSDNVAQLSFDEI"
     terminator      7409..7677
                     /label=Nos
                     /label=NOS terminator
                     /note="NOS terminator"
     misc_feature    7718..7742
                     /label=RB T-DNA
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7870..9212
                     /label=2u ori
                     /note="2u ori"
     protein_bind    complement(8833..8880)
                     /note="FRT"
     promoter        9471..9752
                     /label=S. cerevisiae TRP1 promoter
                     /note="TRP1 promoter"
     CDS             9753..10427
                     /codon_start=1
                     /label=TRP1
                     /note="TRP1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        10763..10768
                     /note="ADH1 promoter"
     CDS             complement(10808..11956)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to
                     and activates oriV"
                     /label=trfA
                     /note="trfA"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12255..13049)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA-3
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    complement(12255..13049)
                     /label=Km
                     /note="Km"
     rep_origin      13261..13892
                     /label=oriV
                     /note="oriV"
                     /note="incP origin of replication"
     misc_feature    complement(13891..14047)
                     /label=LB
                     /note="LB"
     misc_feature    13970..13994
                     /label=LB T-DNA
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcagtc aacatggtgg agcacgacac tctcgtctac tccaagaata
       61 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
      121 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      181 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaaa
      241 gaatgcctct accgacagtg gtcccaaaga tggacccccc acccacgagg aacatcgtgg
      301 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg
      361 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg
      421 ctattgagac tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
      481 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
      541 ttgcgataaa ggaaaggcta tcgttcaaga atgcctctac cgacagtggt cccaaagatg
      601 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      661 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
      721 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaga gcaatcagtg
      781 caaacaaaaa ccttaatcca gacatcttga aattaatcat ataaccatta taatctgagc
      841 agacgtataa gactagtatg gattacaagg accacgacgg tgattataag gatcacgaca
      901 tcgactacaa ggacgatgat gacaagatgg ctccgaagaa gaaaaggaaa gtgggtatac
      961 gagaagcctc tccagcatct ggtcccaggc atctgatgga tccacacatc ttcacatcca
     1021 acttcaacaa cggcatcgga cgacacaaga cgtatctgtg ctacgaggta gagaggttgg
     1081 acaacgggac ttcagtgaag atggaccagc acagaggctt cttgcacaac caagccaaga
     1141 acttgctgtg cggattctac gggaggcatg ccgagttaag attcctcgac ttggttccaa
     1201 gcttgcagct cgacccggca cagatctatc gagtcacgtg gttcatcagt tggtccccgt
     1261 gcttcagttg gggatgtgca ggtgaggtga gagcattcct gcaggagaac actcatgtcc
     1321 gattgcgtat cttcgcagcc cgaatctacg actacgaccc attgtacaaa gaggcactgc
     1381 agatgctcag ggacgccggt gcacaggtct ccatcatgac atacgacgag ttcaagcact
     1441 gttgggacac attcgtcgat catcaaggtt gcccattcca gccttgggac ggtttggacg
     1501 aacacagcca ggctctctct ggaaggctga gggccattct tcagaaccag ggcaactcag
     1561 gatctgaaac gccgggtacc agtgaaagtg ccacacccga gagtatacac ggtgttccag
     1621 cagccgataa gaagtacagt atcggacttg ccatagggac taactctgtc gggtgggcag
     1681 taatcacaga tgagtacaaa gtcccatcta agaagttcaa ggtgctcggt aacaccgata
     1741 gacacagcat caagaagaac ctcatcggtg ctctcctgtt cgattccggc gaaacggccg
     1801 aagccactag gttgaagagg acggcccgac gacgttatac ccgtagaaag aataggatat
     1861 gctatctgca ggagatattc tccaacgaga tggctaaggt agacgatagc ttcttccatc
     1921 gtctcgagga gtccttcctg gtggaagagg acaagaaaca tgaaagacac ccaatctttg
     1981 ggaacattgt tgacgaggtc gcatatcatg agaagtaccc aacaatctac cacttgagga
     2041 agaaactcgt cgacagtact gacaaggctg atctgcgttt aatctactta gcactggctc
     2101 atatgatcaa gttcagaggt cacttcttga tcgagggtga cttgaaccct gacaattcag
     2161 acgtagacaa gctgttcatc cagttagtcc agacgtataa tcagttgttc gaggaaaacc
     2221 caattaacgc tagtggagtc gacgccaagg caatattgag tgcaagactc tccaagtccc
     2281 gaaggttgga aaacctcatt gctcaattgc ctggagagaa gaagaacggg ctcttcggaa
     2341 atcttatagc tctgtcctta gggttaacgc cgaatttcaa gtccaacttc gacctcgccg
     2401 aggacgccaa gttgcaattg tccaaggata cctacgacga tgacctcgac aacttactgg
     2461 ctcagatcgg cgaccagtac gctgacctct tcttggccgc taagaacttg tcagacgcaa
     2521 tcctcttaag tgatatctta cgtgtgaaca cagagattac gaaagctcct ttgagcgcat
     2581 ccatgatcaa gagatacgat gagcaccatc aggatttgac gttgttgaag gccttagttc
     2641 gtcagcaatt gcccgagaag tacaaggaga tcttcttcga tcagtccaag aacggatacg
     2701 cagggtacat cgatggtggt gctagtcaag aggagtttta caagttcatc aagcccatac
     2761 tcgagaagat ggatggaact gaggaactgt tagtgaagct gaatcgtgag gacctgttaa
     2821 ggaagcagcg aacgttcgac aatggcagca tcccgcacca gatccacctt ggcgagctgc
     2881 atgcaatctt gaggagacag gaagacttct atccattctt gaaggacaac agagagaaga
     2941 tcgagaagat cttgacgttc agaatcccgt attacgttgg tccactcgcc aggggtaact
     3001 cccgtttcgc ttggatgacg aggaaatcag aggagacaat cactccttgg aacttcgagg
     3061 aggtggttga caaaggagcc agcgctcaat ccttcataga gcgtatgacc aatttcgaca
     3121 agaatctccc aaacgagaag gtcctgccga aacactcatt gttgtacgag tacttcactg
     3181 tgtataacga gctcacgaag gtcaaatacg taactgaggg tatgcgaaag cccgccttcc
     3241 tctccggtga acagaagaag gcaatcgtcg atttgttgtt caagaccaac aggaaggtca
     3301 ctgtgaagca actgaaagag gactacttca agaagataga gtgcttcgat tctgtggaga
     3361 tcagtggtgt tgaggatcgt ttcaacgcat ccttgggcac ataccatgat ttgttgaaga
     3421 tcatcaagga caaggacttc ctcgacaacg aggagaacga ggacatcctc gaggatatcg
     3481 ttctcacatt gacgctcttc gaggatcgtg agatgatcga ggagagactg aagacctacg
     3541 ctcatttgtt cgatgataag gtgatgaagc aactcaagag gcgtcgatac acgggatggg
     3601 gaaggttgtc tcggaagctg atcaacggta ttagggataa acagtctggc aagacaatct
     3661 tggacttctt gaagagtgat ggattcgcaa ataggaattt catgcagtta atccacgatg
     3721 atagtctgac gttcaaggag gacatccaaa aggcccaagt gagtggtcag ggagacagcc
     3781 tccatgaaca tatagcaaac ttggctggat ccccagcaat taagaaagga atcttgcaga
     3841 cggtcaaagt tgtggatgaa cttgttaagg tcatgggtcg tcacaaacct gagaatatcg
     3901 ttatcgaaat ggcccgagag aatcagacga cccagaaagg acagaagaac tctcgtgaac
     3961 gtatgaaacg aatagaagaa gggatcaaag agctcggtag ccagatcctg aaggagcacc
     4021 cagtagagaa cactcaactg cagaacgaga agctgtatct gtactacttg caaaacgggc
     4081 gagatatgta tgttgaccag gagctcgaca tcaatagact ttctgactac gatgttgacc
     4141 atattgtgcc gcaaagcttc ttgaaggatg atagcataga taacaaagtt ctcacacgtt
     4201 ccgacaagaa cagaggtaag agcgacaacg tgccgagtga agaagtcgtt aagaagatga
     4261 agaactactg gaggcagttg ctcaacgcta agttgatcac ccagaggaag ttcgacaacc
     4321 tcacaaaggc tgagagagga ggcctgtcag agttggataa ggctgggttc atcaagaggc
     4381 agcttgtcga gacacgacag atcaccaagc acgtagccca gatcctcgat tcaaggatga
     4441 acaccaagta cgatgagaac gacaagctca tcagggaagt gaaggtaatc acgctgaaga
     4501 gcaagctcgt atccgacttc cgaaaggact ttcagttcta caaggtgcgt gagatcaaca
     4561 actaccacca tgctcatgat gcatatctta atgcagtggt tgggacggct ctcattaaga
     4621 agtacccaaa gctcgaatct gaatttgtgt atggcgatta caaggtttat gatgtccgta
     4681 agatgattgc taagagcgag caggagattg gtaaggctac cgcgaagtac ttcttctact
     4741 ctaacatcat gaacttcttc aagacggaga tcaccctggc caacggagag atcaggaagc
     4801 gtccgctcat cgagaccaac ggtgagaccg gtgagatcgt gtgggacaag ggacgtgact
     4861 tcgccactgt gaggaaggtg ttgagcatgc cgcaggtgaa catcgtgaag aagacggagg
     4921 tgcagaccgg agggttctcc aaggaatcaa tcttgccaaa gaggaactcc gataaactca
     4981 tagcaaggaa gaaggattgg gatcccaaga agtatggagg gttcgactcc ccgaccgtcg
     5041 cctacagcgt cttggttgtg gcaaaggtgg agaagggaaa gagcaagaag ttgaaatcag
     5101 tcaaggagct ccttggaatc acaatcatgg agcgaagttc tttcgagaag aaccccatag
     5161 acttcttgga ggccaaaggt tacaaagaag tgaagaaaga cttgataatc aagttgccta
     5221 agtactcctt gttcgagctc gagaacggga ggaagcgaat gttggccagc gccggagaac
     5281 ttcagaaagg caacgagctc gctctcccaa gcaagtacgt gaatttcttg tacctcgcat
     5341 cacactacga gaagctgaag ggcagcccgg aggataacga acagaagcag ctcttcgtgg
     5401 agcaacacaa gcactacttg gatgagatca tcgagcagat tagcgagttc agcaagaggg
     5461 taatcttggc tgacgctaat ctggacaagg ttctcagcgc atacaacaag catcgtgaca
     5521 aacccatccg agagcaggca gagaacatca tccacttgtt caccctgact aatttgggag
     5581 cacccgcagc cttcaagtac ttcgatacta ccatcgaccg gaagagatac acaagtacca
     5641 aggaggtcct ggatgcaacc ttgatccatc agtccatcac cggcttgtac gagactagga
     5701 tcgacctctc tcagttgggt ggagacacta gagactccgg aggcagcaca aacctcagcg
     5761 acatcatcga gaaggagaca ggcaagcagt tggtcatcca ggagtctatc ctcatgctgc
     5821 ccgaggaggt tgaggaggtc attggcaata agcctgagag cgacatactt gtgcacaccg
     5881 catatgacga atccacagat gagaatgtga tgcttctgac atcagatgca cctgagtaca
     5941 agccatgggc cctcgtgatc caggactcaa acggtgagaa caagatcaag atgttgagcg
     6001 gcgggtcgcc taagaagaag cgtaaagtgt aacgcgtcga cggctgcttg tcacaagcta
     6061 aatttgatcc tatttcctac attaatcgaa ttatatatat ataaaataaa caaaaaacaa
     6121 acaaaaatac aaataaacaa aaaacaacaa aaagagtctt cggaccaaag tttggctttt
     6181 cagccttatt ttgtttttgt tttttgtttt tgattttttg tttgtttttt atttttgttt
     6241 ttgtttggtt tttgatttaa aaatgcaaaa taaacagaga tttaagctta aataagtggt
     6301 attaaaagta ctatatttca tcaaaagata actgagcaac attatcagaa accccttcct
     6361 cctcttcttc aactgatctc tcaagatttg agctcagttc tttaagctgt tttttaatct
     6421 gcttctcact gtttccttta ggaattatca gcttgcaagc ctcaatgaat gcttgagatc
     6481 tagctctaat agccctgttt agaggtatga ccatgcaact tttgtcttta tcagctctgg
     6541 gtgaatcaca aaactccttt gtccaagaat acatgacact accaaaagaa acccccttct
     6601 tgtattcttg actgcacatc aagtgcagct gacagcagtt ttctggagtg ttattcattt
     6661 tcggaataga ccagttcaga taaaaaacaa aacagatagg atcagttatt gtcccctgac
     6721 ccttcagaat gatttgcttt tcagatggca tgttgggatc gaccagagca accacaagtt
     6781 tccctgtagg gtttggtata gtggggcaga cccatatcac aatcctggaa atcatcatgt
     6841 attgttttct gctgtcccaa gttggacaga tcttgatgac cttattagcg ttttgcttcc
     6901 cgttgccgac aaaaagatca tttttccagt ttgagatatg gtggtttgta tctactatca
     6961 ttcttgcaga aagatcataa ccctctgatt ctgtgataga atcagattca tatgttccaa
     7021 aggaagatgt tccctctgtg ttcaacagta tctttccttt ggatgtatcc atagctttgg
     7081 ttaaagcaag cttttcatca gaagaagacc atggttttga gacttcaaca ttaccgttat
     7141 gtttagcaag tgaaactaaa ggcccttcat cctttctggc agacttagaa gaccccttat
     7201 taccaaaaaa agtcaacatt ttgagttcaa cagcctgagg tagaggagct tgtgtaatgt
     7261 aattgattga ttcttagcaa agtgtataat aggtatattt ctgatgcact gattgctctg
     7321 ggtcggcatg gcatctccac ctcctcgcgg tccgacctgg gcatccgaag gaggacgtcg
     7381 tccactcgga tggctaaggg agagctcgaa tttccccgat cgttcaaaca tttggcaata
     7441 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     7501 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     7561 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     7621 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc ggaattcaga
     7681 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
     7741 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     7801 tatccgttcg tccatttgta tgtgcatgcc aaccacagga gatctcagta aagcgaaaag
     7861 tgccacctga acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg
     7921 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa
     7981 gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga
     8041 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     8101 cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat
     8161 cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc
     8221 tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg
     8281 ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac
     8341 tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc
     8401 tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc
     8461 attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa
     8521 atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt
     8581 ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca
     8641 agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata
     8701 gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc
     8761 gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt
     8821 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc
     8881 aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc
     8941 actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac
     9001 ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa
     9061 aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct
     9121 tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc
     9181 ttcaatgcta tcatttcctt tgatattgga tcatattaag aaaccattat tatcatgaca
     9241 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     9301 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     9361 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     9421 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatagatc aacgacatta
     9481 ctatatatat aatataggaa gcatttaata gaacagcatc gtaatatatg tgtactttgc
     9541 agttatgacg ccagatggca gtagtggaag atattcttta ttgaaaaata gcttgtcacc
     9601 ttacgtacaa tcttgatccg gagcttttct ttttttgccg attaagaatt aattcggtcg
     9661 aaaaaagaaa aggagagggc caagagggag ggcattggtg actattgagc acgtgagtat
     9721 acgtgattaa gcacacaaag gcagcttgga gtatgtctgt tattaatttc acaggtagtt
     9781 ctggtccatt ggtgaaagtt tgcggcttgc agagcacaga ggccgcagaa tgtgctctag
     9841 attccgatgc tgacttgctg ggtattatat gtgtgcccaa tagaaagaga acaattgacc
     9901 cggttattgc aaggaaaatt tcaagtcttg taaaagcata taaaaatagt tcaggcactc
     9961 cgaaatactt ggttggcgtg tttcgtaatc aacctaagga ggatgttttg gctctggtca
    10021 atgattacgg cattgatatc gtccaactgc atggagatga gtcgtggcaa gaataccaag
    10081 agttcctcgg tttgccagtt attaaaagac tcgtatttcc aaaagactgc aacatactac
    10141 tcagtgcagc ttcacagaaa cctcattcgt ttattccctt gtttgattca gaagcaggtg
    10201 ggacaggtga acttttggat tggaactcga tttctgactg ggttggaagg caagagagcc
    10261 ccgaaagctt acattttatg ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc
    10321 ttagattaaa tggcgttatt ggtgttgatg taagcggagg tgtggagaca aatggtgtaa
    10381 aagactctaa caaaatagca aatttcgtca aaaatgctaa gaaataggtt attactgagt
    10441 agtatttatt taagtattgt ttgtgcactt gccgatctat gcggtgtgaa ataccgcaca
    10501 gatgcgtaag gagaaaatac cgcatcagga aattgtaaac gttaatattt tgttaaaatt
    10561 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
    10621 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
    10681 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctcggtgcg
    10741 ggcctcttcg ctattacgcc agatccttgc tggctgaacc cccagccgga actgacccca
    10801 caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc
    10861 cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg
    10921 tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt
    10981 gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc
    11041 atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga
    11101 cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca
    11161 ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg
    11221 acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc
    11281 aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca
    11341 acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga
    11401 tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt
    11461 tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg
    11521 tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg
    11581 tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg
    11641 cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca
    11701 aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca
    11761 gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca
    11821 tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg
    11881 tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt
    11941 caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt
    12001 gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg
    12061 caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct
    12121 tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag
    12181 agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc
    12241 gccacatcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
    12301 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
    12361 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
    12421 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
    12481 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
    12541 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
    12601 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
    12661 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
    12721 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
    12781 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
    12841 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
    12901 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
    12961 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
    13021 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
    13081 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
    13141 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
    13201 acatagtatc gacggagccg attttgaaac cacaattatg ggtgatgctg ccaactcgag
    13261 agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc
    13321 acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa
    13381 agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc
    13441 ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct
    13501 caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc
    13561 gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg
    13621 cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc
    13681 cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc
    13741 atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc
    13801 tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc
    13861 cagcggcgag ggcaaccagc ccggtgagcg tctagtggac tgatgggctg cctgtatcga
    13921 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    13981 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
    14041 tgtactgggg tggtttt
//
