LOCUS       Exported               13154 bp ds-DNA     circular SYN 05-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pM-Cas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Administrator
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 9, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13154
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..767
                     /label=2x35S
                     /note="2x35S"
     CDS             858..5129
                     /codon_start=1
                     /label=3xFlag-Cas9
                     /note="3xFlag-Cas9"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAADKKYS
                     IGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLK
                     RTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD
                     EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDK
                     LFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI
                     ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
                     LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYA
                     GYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGEL
                     HAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNF
                     EEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKP
                     AFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHD
                     LLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRR
                     YTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVS
                     GQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
                     QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINR
                     LSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKL
                     ITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI
                     REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFV
                     YGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGE
                     TGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWD
                     PKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKG
                     YKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKL
                     KGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
                     QAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLS
                     QLGGDKRPAATKKAGQAKKKK"
     misc_feature    861..926
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="3xFLAG"
     misc_feature    933..953
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="SV40 NLS"
     CDS             978..5078
                     /codon_start=1
                     /label=SpCas9
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    3506..3511
                     /label=HindIII
                     /note="HindIII"
     misc_feature    5079..5126
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="nucleoplasmin NLS"
     CDS             complement(5408..6316)
                     /codon_start=1
                     /label=NSm
                     /note="NSm"
                     /translation="MLTFFGNKGSSKSARKDEGPLVSLAKHNGNVEVSKPWSSSDEKLA
                     LTKAMDTSKGKILLNTEGTSSFGTYESDSITESEGYDLSARMIVDTNHHISNWKNDLFV
                     GNGKQNANKVIKICPTWDSRKQYMMISRIVIWVCPTIPNPTGKLVVALVDPNMPSEKQI
                     ILKGQGTITDPICFVFYLNWSIPKMNNTPENCCQLHLMCSQEYKKGVSFGSVMYSWTKE
                     FCDSPRADKDKSCMVIPLNRAIRARSQAFIEACKLIIPKGNSEKQIKKQLKELSSNLER
                     SVEEEEEGVSDNVAQLSFDEI"
     terminator      6506..6774
                     /label=Nos
                     /label=NOS terminator
                     /note="NOS terminator"
     misc_feature    6815..6839
                     /label=RB T-DNA
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6967..8309
                     /label=2u ori
                     /note="2u ori"
     protein_bind    complement(7930..7977)
                     /note="FRT"
     promoter        8568..8849
                     /label=S. cerevisiae TRP1 promoter
                     /note="TRP1 promoter"
     CDS             8850..9524
                     /codon_start=1
                     /label=TRP1
                     /note="TRP1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        9860..9865
                     /note="ADH1 promoter"
     CDS             complement(9905..11053)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to
                     and activates oriV"
                     /label=trfA
                     /note="trfA"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    complement(11352..12146)
                     /label=Km
                     /note="Km"
     CDS             complement(11352..12146)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA-3
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12358..12989
                     /label=oriV
                     /note="oriV"
                     /note="incP origin of replication"
     misc_feature    complement(12988..13144)
                     /label=LB
                     /note="LB"
     misc_feature    13067..13091
                     
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcagtc aacatggtgg agcacgacac tctcgtctac tccaagaata
       61 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
      121 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      181 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaaa
      241 gaatgcctct accgacagtg gtcccaaaga tggacccccc acccacgagg aacatcgtgg
      301 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg
      361 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccagaggg
      421 ctattgagac tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
      481 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
      541 ttgcgataaa ggaaaggcta tcgttcaaga atgcctctac cgacagtggt cccaaagatg
      601 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      661 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
      721 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaga gcaatcagtg
      781 caaacaaaaa ccttaatcca gacatcttga aattaatcat ataaccatta taatctgagc
      841 agacgtataa gactagtatg gattacaagg accacgacgg tgattataag gatcacgaca
      901 tcgactacaa ggacgatgat gacaagatgg ctccgaagaa gaaaaggaaa gtgggtatac
      961 acggtgttcc agcagccgat aagaagtaca gtatcggact tgacataggg actaactctg
     1021 tcgggtgggc agtaatcaca gatgagtaca aagtcccatc taagaagttc aaggtgctcg
     1081 gtaacaccga tagacacagc atcaagaaga acctcatcgg tgctctcctg ttcgattccg
     1141 gcgaaacggc cgaagccact aggttgaaga ggacggcccg acgacgttat acccgtagaa
     1201 agaataggat atgctatctg caggagatat tctccaacga gatggctaag gtagacgata
     1261 gcttcttcca tcgtctcgag gagtccttcc tggtggaaga ggacaagaaa catgaaagac
     1321 acccaatctt tgggaacatt gttgacgagg tcgcatatca tgagaagtac ccaacaatct
     1381 accacttgag gaagaaactc gtcgacagta ctgacaaggc tgatctgcgt ttaatctact
     1441 tagcactggc tcatatgatc aagttcagag gtcacttctt gatcgagggt gacttgaacc
     1501 ctgacaattc agacgtagac aagctgttca tccagttagt ccagacgtat aatcagttgt
     1561 tcgaggaaaa cccaattaac gctagtggag tcgacgccaa ggcaatattg agtgcaagac
     1621 tctccaagtc ccgaaggttg gaaaacctca ttgctcaatt gcctggagag aagaagaacg
     1681 ggctcttcgg aaatcttata gctctgtcct tagggttaac gccgaatttc aagtccaact
     1741 tcgacctcgc cgaggacgcc aagttgcaat tgtccaagga tacctacgac gatgacctcg
     1801 acaacttact ggctcagatc ggcgaccagt acgctgacct cttcttggcc gctaagaact
     1861 tgtcagacgc aatcctctta agtgatatct tacgtgtgaa cacagagatt acgaaagctc
     1921 ctttgagcgc atccatgatc aagagatacg atgagcacca tcaggatttg acgttgttga
     1981 aggccttagt tcgtcagcaa ttgcccgaga agtacaagga gatcttcttc gatcagtcca
     2041 agaacggata cgcagggtac atcgatggtg gtgctagtca agaggagttt tacaagttca
     2101 tcaagcccat actcgagaag atggatggaa ctgaggaact gttagtgaag ctgaatcgtg
     2161 aggacctgtt aaggaagcag cgaacgttcg acaatggcag catcccgcac cagatccacc
     2221 ttggcgagct gcatgcaatc ttgaggagac aggaagactt ctatccattc ttgaaggaca
     2281 acagagagaa gatcgagaag atcttgacgt tcagaatccc gtattacgtt ggtccactcg
     2341 ccaggggtaa ctcccgtttc gcttggatga cgaggaaatc agaggagaca atcactcctt
     2401 ggaacttcga ggaggtggtt gacaaaggag ccagcgctca atccttcata gagcgtatga
     2461 ccaatttcga caagaatctc ccaaacgaga aggtcctgcc gaaacactca ttgttgtacg
     2521 agtacttcac tgtgtataac gagctcacga aggtcaaata cgtaactgag ggtatgcgaa
     2581 agcccgcctt cctctccggt gaacagaaga aggcaatcgt cgatttgttg ttcaagacca
     2641 acaggaaggt cactgtgaag caactgaaag aggactactt caagaagata gagtgcttcg
     2701 attctgtgga gatcagtggt gttgaggatc gtttcaacgc atccttgggc acataccatg
     2761 atttgttgaa gatcatcaag gacaaggact tcctcgacaa cgaggagaac gaggacatcc
     2821 tcgaggatat cgttctcaca ttgacgctct tcgaggatcg tgagatgatc gaggagagac
     2881 tgaagaccta cgctcatttg ttcgatgata aggtgatgaa gcaactcaag aggcgtcgat
     2941 acacgggatg gggaaggttg tctcggaagc tgatcaacgg tattagggat aaacagtctg
     3001 gcaagacaat cttggacttc ttgaagagtg atggattcgc aaataggaat ttcatgcagt
     3061 taatccacga tgatagtctg acgttcaagg aggacatcca aaaggcccaa gtgagtggtc
     3121 agggagacag cctccatgaa catatagcaa acttggctgg atccccagca attaagaaag
     3181 gaatcttgca gacggtcaaa gttgtggatg aacttgttaa ggtcatgggt cgtcacaaac
     3241 ctgagaatat cgttatcgaa atggcccgag agaatcagac gacccagaaa ggacagaaga
     3301 actctcgtga acgtatgaaa cgaatagaag aagggatcaa agagctcggt agccagatcc
     3361 tgaaggagca cccagtagag aacactcaac tgcagaacga gaagctgtat ctgtactact
     3421 tgcaaaacgg gcgagatatg tatgttgacc aggagctcga catcaataga ctttctgact
     3481 acgatgttga ccatattgtg ccgcaaagct tcttgaagga tgatagcata gataacaaag
     3541 ttctcacacg ttccgacaag aacagaggta agagcgacaa cgtgccgagt gaagaagtcg
     3601 ttaagaagat gaagaactac tggaggcagt tgctcaacgc taagttgatc acccagagga
     3661 agttcgacaa cctcacaaag gctgagagag gaggcctgtc agagttggat aaggctgggt
     3721 tcatcaagag gcagcttgtc gagacacgac agatcaccaa gcacgtagcc cagatcctcg
     3781 attcaaggat gaacaccaag tacgatgaga acgacaagct catcagggaa gtgaaggtaa
     3841 tcacgctgaa gagcaagctc gtatccgact tccgaaagga ctttcagttc tacaaggtgc
     3901 gtgagatcaa caactaccac catgctcatg atgcatatct taatgcagtg gttgggacgg
     3961 ctctcattaa gaagtaccca aagctcgaat ctgaatttgt gtatggcgat tacaaggttt
     4021 atgatgtccg taagatgatt gctaagagcg agcaggagat tggtaaggct accgcgaagt
     4081 acttcttcta ctctaacatc atgaacttct tcaagacgga gatcaccctg gccaacggag
     4141 agatcaggaa gcgtccgctc atcgagacca acggtgagac cggtgagatc gtgtgggaca
     4201 agggacgtga cttcgccact gtgaggaagg tgttgagcat gccgcaggtg aacatcgtga
     4261 agaagacgga ggtgcagacc ggagggttct ccaaggaatc aatcttgcca aagaggaact
     4321 ccgataaact catagcaagg aagaaggatt gggatcccaa gaagtatgga gggttcgact
     4381 ccccgaccgt cgcctacagc gtcttggttg tggcaaaggt ggagaaggga aagagcaaga
     4441 agttgaaatc agtcaaggag ctccttggaa tcacaatcat ggagcgaagt tctttcgaga
     4501 agaaccccat agacttcttg gaggccaaag gttacaaaga agtgaagaaa gacttgataa
     4561 tcaagttgcc taagtactcc ttgttcgagc tcgagaacgg gaggaagcga atgttggcca
     4621 gcgccggaga acttcagaaa ggcaacgagc tcgctctccc aagcaagtac gtgaatttct
     4681 tgtacctcgc atcacactac gagaagctga agggcagccc ggaggataac gaacagaagc
     4741 agctcttcgt ggagcaacac aagcactact tggatgagat catcgagcag attagcgagt
     4801 tcagcaagag ggtaatcttg gctgacgcta atctggacaa ggttctcagc gcatacaaca
     4861 agcatcgtga caaacccatc cgagagcagg cagagaacat catccacttg ttcaccctga
     4921 ctaatttggg agcacccgca gccttcaagt acttcgatac taccatcgac cggaagagat
     4981 acacaagtac caaggaggtc ctggatgcaa ccttgatcca tcagtccatc accggcttgt
     5041 acgagactag gatcgacctc tctcagttgg gtggagacaa gcgaccggca gccacgaaga
     5101 aagcaggcca ggccaagaag aagaagtaac gcgtcgacgg ctgcttgtca caagctaaat
     5161 ttgatcctat ttcctacatt aatcgaatta tatatatata aaataaacaa aaaacaaaca
     5221 aaaatacaaa taaacaaaaa acaacaaaaa gagtcttcgg accaaagttt ggcttttcag
     5281 ccttattttg tttttgtttt ttgtttttga ttttttgttt gttttttatt tttgtttttg
     5341 tttggttttt gatttaaaaa tgcaaaataa acagagattt aagcttaaat aagtggtatt
     5401 aaaagtacta tatttcatca aaagataact gagcaacatt atcagaaacc ccttcctcct
     5461 cttcttcaac tgatctctca agatttgagc tcagttcttt aagctgtttt ttaatctgct
     5521 tctcactgtt tcctttagga attatcagct tgcaagcctc aatgaatgct tgagatctag
     5581 ctctaatagc cctgtttaga ggtatgacca tgcaactttt gtctttatca gctctgggtg
     5641 aatcacaaaa ctcctttgtc caagaataca tgacactacc aaaagaaacc cccttcttgt
     5701 attcttgact gcacatcaag tgcagctgac agcagttttc tggagtgtta ttcattttcg
     5761 gaatagacca gttcagataa aaaacaaaac agataggatc agttattgtc ccctgaccct
     5821 tcagaatgat ttgcttttca gatggcatgt tgggatcgac cagagcaacc acaagtttcc
     5881 ctgtagggtt tggtatagtg gggcagaccc atatcacaat cctggaaatc atcatgtatt
     5941 gttttctgct gtcccaagtt ggacagatct tgatgacctt attagcgttt tgcttcccgt
     6001 tgccgacaaa aagatcattt ttccagtttg agatatggtg gtttgtatct actatcattc
     6061 ttgcagaaag atcataaccc tctgattctg tgatagaatc agattcatat gttccaaagg
     6121 aagatgttcc ctctgtgttc aacagtatct ttcctttgga tgtatccata gctttggtta
     6181 aagcaagctt ttcatcagaa gaagaccatg gttttgagac ttcaacatta ccgttatgtt
     6241 tagcaagtga aactaaaggc ccttcatcct ttctggcaga cttagaagac cccttattac
     6301 caaaaaaagt caacattttg agttcaacag cctgaggtag aggagcttgt gtaatgtaat
     6361 tgattgattc ttagcaaagt gtataatagg tatatttctg atgcactgat tgctctgggt
     6421 cggcatggca tctccacctc ctcgcggtcc gacctgggca tccgaaggag gacgtcgtcc
     6481 actcggatgg ctaagggaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
     6541 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     6601 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     6661 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     6721 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgga attcagattg
     6781 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     6841 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     6901 ccgttcgtcc atttgtatgt gcatgccaac cacaggagat ctcagtaaag cgaaaagtgc
     6961 cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta
     7021 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg
     7081 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag
     7141 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     7201 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc
     7261 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct
     7321 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta
     7381 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga
     7441 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat
     7501 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt
     7561 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg
     7621 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
     7681 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
     7741 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
     7801 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt
     7861 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa
     7921 aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa
     7981 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact
     8041 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc
     8101 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg
     8161 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca
     8221 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc
     8281 aatgctatca tttcctttga tattggatca tattaagaaa ccattattat catgacatta
     8341 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     8401 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     8461 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     8521 aactatgcgg catcagagca gattgtactg agagtgcacc atagatcaac gacattacta
     8581 tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt actttgcagt
     8641 tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct tgtcacctta
     8701 cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat tcggtcgaaa
     8761 aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg tgagtatacg
     8821 tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca ggtagttctg
     8881 gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt gctctagatt
     8941 ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca attgacccgg
     9001 ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca ggcactccga
     9061 aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct ctggtcaatg
     9121 attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa taccaagagt
     9181 tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac atactactca
     9241 gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa gcaggtggga
     9301 caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa gagagccccg
     9361 aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt gatgcgctta
     9421 gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag
     9481 actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt actgagtagt
     9541 atttatttaa gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata ccgcacagat
     9601 gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt taaaattcgc
     9661 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     9721 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     9781 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct cggtgcgggc
     9841 ctcttcgcta ttacgccaga tccttgctgg ctgaaccccc agccggaact gaccccacaa
     9901 ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc
     9961 tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg
    10021 aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg
    10081 agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata
    10141 tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct
    10201 ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg
    10261 attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca
    10321 ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa
    10381 gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca
    10441 cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct
    10501 tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct
    10561 tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt
    10621 ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt
    10681 gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg
    10741 tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac
    10801 ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg
    10861 cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg
    10921 agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt
    10981 cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa
    11041 acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc
    11101 cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa
    11161 tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc
    11221 cctgcacgaa taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc
    11281 caaaacactt gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc
    11341 acatctaggt actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca
    11401 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc
    11461 ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca
    11521 agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg
    11581 ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg
    11641 cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg
    11701 ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa
    11761 tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt
    11821 tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg
    11881 agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt
    11941 ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac
    12001 cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta
    12061 gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat
    12121 tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa
    12181 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
    12241 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
    12301 tagtatcgac ggagccgatt ttgaaaccac aattatgggt gatgctgcca actcgagagc
    12361 gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca
    12421 gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga
    12481 caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg
    12541 tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa
    12601 gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca
    12661 gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt
    12721 tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct
    12781 catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc
    12841 tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg
    12901 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag
    12961 cggcgagggc aaccagcccg gtgagcgtct agtggactga tgggctgcct gtatcgagtg
    13021 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    13081 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    13141 actggggtgg tttt
//
