LOCUS       pGA-red-mini        3534 bp DNA     circular SYN 02-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Pietro Butti
  TITLE     Direct Submission
  JOURNAL   Exported Jan 2, 2023 from SnapGene 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             153..1013
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1184..1772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2060..2081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2096..2126
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2134..2150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2158..2174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2187..2217
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     protein_bind    2246..2267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2282..2312
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2320..2336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2354..2365
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2372..3049
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3094..3165
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3181..3208
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    3239..3245
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    3243..3398
                     /label=lacZ_a
     primer_bind     complement(3246..3262)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaaaaatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
       61 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      121 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      181 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      241 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      301 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      361 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      421 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      481 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      541 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      601 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      661 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
      721 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
      781 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
      841 ctgataaatc tggagccggt gagcgtggat ctcgcggtat cattgcagca ctggggccag
      901 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
      961 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1021 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1081 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1141 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1201 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1261 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1321 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1381 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1441 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1501 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1561 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1621 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1681 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1741 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1801 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1861 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     1921 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     1981 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2041 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2101 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2161 gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat
     2221 ccgctagctg ccagagaccc gcaattaatg tgagttagct cactcattag gcaccccagg
     2281 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2341 acacatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga
     2401 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga
     2461 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa
     2521 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa
     2581 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg
     2641 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga
     2701 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc
     2761 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat
     2821 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg
     2881 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc
     2941 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat
     3001 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata
     3061 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     3121 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     3181 ggctcacctt cgggtgggcc tttctgcgtt tatataggtc tctgcaagct agcgagctcg
     3241 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     3301 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     3361 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     3421 ctccttacgc atctgtgcgg tatttcacac cgcatatatc gctgggccat tctcatgaag
     3481 aatatcttga atttattgtc atattactag ttggtgtgga agtccatata tcgg
//
