LOCUS       pGTVL2-SENP5-AF1        6811 bp ds-DNA     circular     06-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     2526..2545
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2526..2545
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    2545..2567
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_binding    2545..2567
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2575..2640
                     /label="Translation 2575-2640"
     misc_feature    2641..3294
                     /label="GST"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             2641..3294
                     /label="Translation 2641-3294"
     CDS             3295..4214
                     /label="Translation 3295-4214"
     primer          complement(3317..3345)
                     /label="pGTVL2_lin_rv"
                     /note="sequence: catggattggaagtacaggttctcggtac"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          3327..3367
                     /label="hSENP5-AF1_fw"
                     /note="sequence: cctgtacttccaatccatgAGTCCGGTGGATGACGAACAGC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3346..4137
                     /label="AlphaFold"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3478..4137
                     /label="SENP5-catalytic domain"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(4114..4157)
                     /label="hSENP5-AF1_rv"
                     /note="sequence: cggatccgtatccacctttaGTCCATCAGGCGACATTCACACAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          4138..4165
                     /label="pGTVL2_lin_fw2"
                     /note="sequence: taaaggtggatacggatccgaattcgag"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             4138..4214
                     /label="Translation 4138-4214"
     CDS             4197..4214
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             4197..4214
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      4365..4820
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      4365..4820
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      4382..4688
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      4382..4688
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(4916..5728)
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(4916..5728)
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      5774..6456
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      5774..6456
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
       61 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
      121 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
      181 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
      241 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
      301 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
      361 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
      421 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
      481 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
      541 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
      601 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
      661 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc
      721 gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg
      781 aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc
      841 gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct
      901 acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc
      961 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
     1021 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1081 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1141 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     1201 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     1261 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     1321 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     1381 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     1441 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     1501 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
     1561 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
     1621 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
     1681 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
     1741 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
     1801 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
     1861 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     1921 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
     1981 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     2041 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     2101 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     2161 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
     2221 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     2281 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     2341 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     2401 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     2461 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc
     2521 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat
     2581 aattttgttt aactttaaga aggagatata catatgcacc atcatcatca tcattcttct
     2641 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
     2701 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
     2761 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
     2821 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
     2881 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
     2941 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
     3001 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
     3061 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
     3121 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
     3181 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
     3241 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcgagc
     3301 tcaggtgtag atctgggtac cgagaacctg tacttccaat ccatgAGTCC GGTGGATGAC
     3361 GAACAGCTGT CCGTTTGCCT GTCAGGCTTT CTGGATGAAG TGATGAAAAA ATATGGTTCT
     3421 CTGGTCCCGC TGAGTGAAAA AGAAGTGCTG GGTCGCCTGA AAGATGTTTT TAACGAAGAC
     3481 TTCAGCAATC GCAAACCGTT TATTAACCGT GAAATCACCA ATTATCGCGC GCGTCATCAG
     3541 AAATGTAACT TCCGTATTTT CTACAACAAA CACATGCTGG ATATGGATGA CCTGGCCACC
     3601 CTGGATGGCC AAAACTGGCT GAATGACCAG GTTATTAACA TGTATGGTGA ACTGATCATG
     3661 GATGCGGTGC CGGACAAAGT TCATTTCTTT AACAGCTTTT TCCACCGCCA GCTGGTCACG
     3721 AAAGGTTACA ATGGCGTGAA ACGTTGGACC AAAAAAGTTG ACCTGTTTAA AAAATCTCTG
     3781 CTGCTGATTC CGATCCATCT GGAAGTCCAC TGGTCCCTGA TTACCGTGAC GCTGTCAAAC
     3841 CGCATTATCT CGTTCTATGA TAGCCAGGGT ATCCATTTCA AATTCTGCGT GGAAAACATC
     3901 CGTAAATACC TGCTGACCGA AGCGCGTGAG AAAAACCGTC CGGAATTTCT GCAGGGCTGG
     3961 CAAACCGCTG TTACGAAATG CATCCCGCAG CAGAAAAACG ATAGCGACTG TGGTGTTTTC
     4021 GTCCTGCAGT ACTGCAAATG TCTGGCCCTG GAACAGCCGT TTCAATTCTC ACAGGAAGAT
     4081 ATGCCGCGTG TGCGTAAACG TATCTACAAA GAACTGTGTG AATGTCGCCT GATGGACtaa
     4141 aggtggatac ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc
     4201 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     4261 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     4321 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
     4381 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4441 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4501 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4561 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4621 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4681 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4741 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4801 aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4861 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa
     4921 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     4981 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     5041 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     5101 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     5161 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
     5221 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     5281 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     5341 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     5401 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     5461 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     5521 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     5581 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     5641 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc
     5701 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     5761 acagttttat tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5821 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5881 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5941 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     6001 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6061 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6121 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6181 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6241 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6301 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6361 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6421 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6481 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6541 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6601 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     6661 atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     6721 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     6781 aacacccgct gacgcgccct gacgggcttg t
//