LOCUS       pGTVL2-SENP6-AF1        7357 bp ds-DNA     circular     06-AUG-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     2526..2545
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2526..2545
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    2545..2567
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_binding    2545..2567
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2575..2640
                     /label="Translation 2575-2640"
     misc_feature    2641..3294
                     /label="GST"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             2641..3294
                     /label="Translation 2641-3294"
     CDS             3295..4760
                     /label="Translation 3295-4760"
     primer          complement(3317..3345)
                     /label="pGTVL2_lin_rv"
                     /note="sequence: catggattggaagtacaggttctcggtac"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          3327..3370
                     /label="hSENP6-AF1_fw"
                     /note="sequence: cctgtacttccaatccatgGAAAAACTGATTGTGTATCCGCCGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3346..4683
                     /label="AlphaFold-domain-long"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3820..4080
                     /label="Loop deletion"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(4652..4703)
                     /label="hSENP6-AF1_rv"
                     /note="sequence: cggatccgtatccacctttaAGCTTCGGTTGAATACGTATCTTTGTGTTTAC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          4684..4711
                     /label="pGTVL2_lin_fw2"
                     /note="sequence: taaaggtggatacggatccgaattcgag"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             4684..4760
                     /label="Translation 4684-4760"
     CDS             4743..4760
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             4743..4760
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      4911..5366
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      4911..5366
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      4928..5234
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      4928..5234
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(5462..6274)
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(5462..6274)
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      6320..7002
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      6320..7002
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
       61 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
      121 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
      181 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
      241 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
      301 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
      361 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
      421 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
      481 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
      541 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
      601 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
      661 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc
      721 gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg
      781 aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc
      841 gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct
      901 acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc
      961 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
     1021 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1081 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1141 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     1201 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     1261 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     1321 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     1381 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     1441 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     1501 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
     1561 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
     1621 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
     1681 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
     1741 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
     1801 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
     1861 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     1921 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
     1981 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     2041 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     2101 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     2161 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
     2221 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     2281 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     2341 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     2401 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     2461 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc
     2521 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat
     2581 aattttgttt aactttaaga aggagatata catatgcacc atcatcatca tcattcttct
     2641 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
     2701 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
     2761 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
     2821 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
     2881 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
     2941 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
     3001 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
     3061 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
     3121 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
     3181 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
     3241 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcgagc
     3301 tcaggtgtag atctgggtac cgagaacctg tacttccaat ccatgGAAAA ACTGATTGTG
     3361 TATCCGCCGC CGCCGGCCAA AGGCGGTATC AGCGTGACCA ATGAAGATCT GCACTGCCTG
     3421 AACGAGGGTG AATTTCTGAA TGACGTGATT ATCGATTTCT ATCTGAAATA CCTGGTTCTG
     3481 GAAAAACTGA AAAAAGAAGA CGCCGATCGC ATCCATATCT TTTCATCGTT TTTCTATAAA
     3541 CGCCTGAACC AGCGTGAACG TCGCAACCAC GAAACCACGA ATCTGAGCAT TCAGCAAAAA
     3601 CGCCATGGCC GTGTGAAAAC CTGGACGCGT CACGTTGACA TCTTCGAAAA AGATTTCATC
     3661 TTCGTTCCGC TGAATGAAGC GGCCCATTGG TTTCTGGCAG TGGTTTGCTT CCCGGGTCTG
     3721 GAAAAACCGA AATATGAACC GAACCCGCAT TACCACGAAA ATGCTGTCAT TCAGAAATGC
     3781 TCAACCGTGG AAGATTCGTG TATCAGCTCT AGTGCGAGCG AAATGGAATC TTGCAGTCAG
     3841 AACTCCTCAG CCAAACCGGT TATTAAGAAA ATGCTGAACA AAAAACATTG TATTGCGGTC
     3901 ATCGACTCTA ATCCGGGCCA GGAAGAAAGT GATCCGCGCT ACAAACGTAA CATTTGTTCC
     3961 GTTAAATACT CAGTGAAGAA AATTAACCAC ACCGCGAGCG AAAACGAAGA ATTCAACAAG
     4021 GGTGAATCCA CCTCACAGAA AGTGGCCGAT CGCACGAAAT CGGAAAACGG CCTGCAAAAT
     4081 GAAAGCCTGT CGAGCACCCA TCACACGGAC GGTCTGTCGA AAATTCGTCT GAACTACTCG
     4141 GATGAAAGCC CGGAAGCCGG CAAAATGCTG GAAGACGAAC TGGTTGATTT TTCAGAAGAC
     4201 CAGGATAACC AAGACGATTC TAGTGACGAT GGCTTCCTGG CAGACGATAA TTGCTCCTCA
     4261 GAAATTGGCC AGTGGCATCT GAAACCGACC ATTTGCAAAC AACCGTGTAT CCTGCTGATG
     4321 GATTCTCTGC GCGGCCCGTC GCGTAGCAAT GTCGTGAAAA TCCTGCGCGA ATATCTGGAA
     4381 GTGGAATGGG AAGTTAAAAA AGGCAGTAAA CGTTCTTTCA GTAAAGATGT CATGAAAGGT
     4441 TCCAACCCGA AAGTGCCGCA GCAAAACAAT TTCTCAGATT GTGGTGTTTA TGTCCTGCAG
     4501 TACGTGGAAA GCTTTTTCGA AAATCCGATT CTGAGTTTTG AACTGCCGAT GAACCTGGCG
     4561 AATTGGTTTC CGCCGCCGCG TATGCGCACC AAACGCGAAG AAATCCGTAA TATTATCCTG
     4621 AAACTGCAGG AAGACCAAAG CAAAGAAAAA CGTAAACACA AAGATACGTA TTCAACCGAA
     4681 GCTtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg
     4741 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
     4801 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     4861 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
     4921 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4981 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5041 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5101 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5161 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5221 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5281 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5341 taacaaaata ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac
     5401 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt
     5461 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     5521 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     5581 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     5641 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     5701 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     5761 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     5821 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     5881 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     5941 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
     6001 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     6061 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     6121 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     6181 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     6241 tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     6301 aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga
     6361 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6421 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6481 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6541 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6601 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6661 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6721 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6781 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6841 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6901 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6961 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7021 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7081 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7141 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     7201 tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat
     7261 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     7321 cccgccaaca cccgctgacg cgccctgacg ggcttgt
//