LOCUS       pNIC28-BIRC3_FL         7171 bp ds-DNA     linear       10-DEC-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    45..62
                     /label="N-His"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(85..110)
                     /label="pNIC_lin_rv"
                     /note="sequence: catggattggaagtacaggttctcgg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          91..155
                     /label="pNIC28_BIRC3_FL_fw"
                     /note="sequence: acctgtacttccaatccatgaacatagtagaaaacagcatattcttatcaaatttgatgaaaagc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    108..110
                     /label="start"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             108..1922
                     /label="Translation 108-1922"
     CDS             111..1919
                     /label="BIRC3 sequence"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    192..395
                     /label="bir1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    612..812
                     /label="bir2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    837..1118
                     /label="Xray (2uvl)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    870..1073
                     /label="bir3"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1887..1942)
                     /label="pNIC28_BIRC3_FL_rv"
                     /note="sequence: atccgtatccacctttactgTTAtgaaagaaatgtacgaactgtacccttgattgt"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          1923..1951
                     /label="pNIC_lin_fw"
                     /note="sequence: cagtaaaggtggatacggatccgaattcg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1985..2002
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      2153..2608
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      2170..2476
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(2704..3516)
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      3562..4244
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     7125..7144
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    7144..7166
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc
       61 attcttctgg tgtagatctg ggtaccgaga acctgtactt ccaatccatg aacatagtag
      121 aaaacagcat attcttatca aatttgatga aaagcgccaa cacgtttgaa ctgaaatacg
      181 acttgtcatg tgaactgtac cgaatgtcta cgtattccac ttttcctgct ggggttcctg
      241 tctcagaaag gagtcttgct cgtgctggtt tctattacac tggtgtgaat gacaaggtca
      301 aatgcttctg ttgtggcctg atgctggata actggaaaag aggagacagt cctactgaaa
      361 agcataaaaa gttgtatcct agctgcagat tcgttcagag tctaaattcc gttaacaact
      421 tggaagctac ctctcagcct acttttcctt cttcagtaac aaattccaca cactcattac
      481 ttccgggtac agaaaacagt ggatatttcc gtggctctta ttcaaactct ccatcaaatc
      541 ctgtaaactc cagagcaaat caagattttt ctgccttgat gagaagttcc taccactgtg
      601 caatgaataa cgaaaatgcc agattactta cttttcagac atggccattg acttttctgt
      661 cgccaacaga tctggcaaaa gcaggctttt actacatagg acctggagac agagtggctt
      721 gctttgcctg tggtggaaaa ttgagcaatt gggaaccgaa ggataatgct atgtcagaac
      781 acctgagaca ttttcccaaa tgcccattta tagaaaatca gcttcaagac acttcaagat
      841 acacagtttc taatctgagc atgcagacac atgcagcccg ctttaaaaca ttctttaact
      901 ggccctctag tgttctagtt aatcctgagc agcttgcaag tgcgggtttt tattatgtgg
      961 gtaacagtga tgatgtcaaa tgcttttgct gtgatggtgg actcaggtgt tgggaatctg
     1021 gagatgatcc atgggttcaa catgccaagt ggtttccaag gtgtgagtac ttgataagaa
     1081 ttaaaggaca ggagttcatc cgtcaagttc aagccagtta ccctcatcta cttgaacagc
     1141 tgctatccac atcagacagc ccaggagatg aaaatgcaga gtcatcaatt atccattttg
     1201 aacctggaga agaccattca gaagatgcaa tcatgatgaa tactcctgtg attaatgctg
     1261 ccgtggaaat gggctttagt agaagcctgg taaaacagac agttcagaga aaaatcctag
     1321 caactggaga gaattataga ctagtcaatg atcttgtgtt agacttactc aatgcagaag
     1381 atgaaataag ggaagaggag agagaaagag caactgagga aaaagaatca aatgatttat
     1441 tattaatccg gaagaataga atggcacttt ttcaacattt gacttgtgta attccaatcc
     1501 tggatagtct actaactgcc ggaattatta atgaacaaga acatgatgtt attaaacaga
     1561 agacacagac gtctttacaa gcaagagaac tgattgatac gattttagta aaaggaaata
     1621 ttgcagccac tgtattcaga aactctctgc aagaagctga agctgtgtta tatgagcatt
     1681 tatttgtgca acaggacata aaatatattc ccacagaaga tgtttcagat ctaccagtgg
     1741 aagaacaatt gcggagacta caagaagaaa gaacatgtaa agtgtgtatg gacaaagaag
     1801 tgtccatagt gtttattcct tgtggtcatc tagtagtatg caaagattgt gctccttctt
     1861 taagaaagtg tcctatttgt aggagtacaa tcaagggtac agttcgtaca tttctttcaT
     1921 AAcagtaaag gtggatacgg atccgaattc gagctccgtc gacaagcttg cggccgcact
     1981 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
     2041 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     2101 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
     2161 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2221 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2281 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2341 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2401 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2461 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     2521 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2581 tttaacaaaa tattaacgtt tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga
     2641 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
     2701 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     2761 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     2821 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     2881 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     2941 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     3001 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     3061 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     3121 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     3181 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     3241 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     3301 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     3361 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     3421 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     3481 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     3541 gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact
     3601 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3661 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3721 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3781 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3841 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3901 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3961 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4021 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4081 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4141 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4201 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4261 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4321 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4381 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     4441 tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc
     4501 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     4561 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4621 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4681 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     4741 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     4801 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     4861 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     4921 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     4981 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     5041 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     5101 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     5161 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     5221 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     5281 ctcaacgaca ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc
     5341 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc
     5401 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
     5461 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
     5521 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
     5581 ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca
     5641 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5701 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
     5761 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
     5821 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
     5881 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc
     5941 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
     6001 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg
     6061 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
     6121 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
     6181 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
     6241 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
     6301 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
     6361 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
     6421 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
     6481 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
     6541 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
     6601 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     6661 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     6721 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     6781 cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat
     6841 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
     6901 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     6961 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     7021 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     7081 cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat
     7141 aggggaattg tgagcggata acaattcccc t
//