LOCUS       AAV-GfaABC1D-Cre-4x6T   5893 bp ds-DNA     circular     09-DEC-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..629
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    673..802
                     /label="AAV2 ITR "
                     /ApEinfo_revcolor="#00c72e"
                     /ApEinfo_fwdcolor="#00c72e"
     misc_feature    817..1496
                     /label="GfaABC1D"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1582..2613
                     /label="Cre"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2620..2639
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    2620..3148
                     /label="4x6T miR cassette"
                     /ApEinfo_revcolor="#00c800"
                     /ApEinfo_fwdcolor="#00c800"
     misc_feature    2640..2662
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    2663..2684
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2685..2706
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    2707..2728
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    2729..2750
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    2751..2772
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    2773..2792
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    2793..2815
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    2816..2837
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2838..2859
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    2860..2881
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    2882..2901
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    2902..2924
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    2925..2946
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2947..2968
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    2969..2990
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    2996..3017
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3018..3039
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3040..3059
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    3060..3082
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    3083..3104
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3105..3126
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3127..3148
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     polyA_signal    3305..3785
                     /label="hGH polyA signal"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3828..3969
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#00c636"
                     /ApEinfo_fwdcolor="#00c636"
     CDS             5083..5742
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt
      721 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      781 aactccatca ctaggggttc ctgcggccgc acgcgtacat atcctggtgt ggagtagggg
      841 acgctgctct gacagaggct cgggggcctg agctggctct gtgagctggg gaggaggcag
      901 acagccaggc cttgtctgca agcagacctg gcagcattgg gctggccgcc ccccagggcc
      961 tcctcttcat gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac
     1021 agtgcctgct tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag
     1081 cagggggctt gcattgcacc ccagcctgac agcctggcat cttgggataa aagcagcaca
     1141 gccccctagg ggctgccctt gctgtgtggc gccaccggcg gtggagaaca aggctctatt
     1201 cagcctgtgc ccaggaaagg ggatcagggg atgcccaggc atggacagtg ggtggcaggg
     1261 ggggagagga gggctgtctg cttcccagaa gtccaaggac acaaatgggt gaggggagag
     1321 ctctccccat agctgggctg cggcccaacc ccaccccctc aggctatgcc agggggtgtt
     1381 gccaggggca cccgggcatc gccagtctag cccactcctt cataaagccc tcgcatccca
     1441 ggagcgagca gagccagagc aggttggaga ggagacgcat cacctccgct gctcgcttaa
     1501 ttaatacctg aattctgcag attcgagcgg ccgccagtgt gatggatatc tgcagaattc
     1561 aggtggccaa gatctgccac catggccaat ttactgaccg tacaccaaaa tttgcctgca
     1621 ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat gttcagggat
     1681 cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg
     1741 gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat
     1801 tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca acatttgggc
     1861 cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag caatgctgtt
     1921 tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag
     1981 gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga aaatagcgat
     2041 cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac cctgttacgt
     2101 atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg tgggagaatg
     2161 ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga gaaggcactt
     2221 agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt agctgatgat
     2281 ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc
     2341 agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg attgatttac
     2401 ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc
     2461 ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt
     2521 ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg
     2581 gcaatggtgc gcctgctgga agatggcgat tagcatatgg gcattcaccg cgtgccttac
     2641 tacgcgtatt cttaagcaat aagcgaatat aacacggtcg atctaaaaag gttagctggg
     2701 tgtgttagaa gccctgcaag acgacctgcg cattattact cacggtacga cgcattatta
     2761 ctcacggtac gaggcattca ccgcgtgcct tactacgcgt attcttaagc aataagcgaa
     2821 tataacacgg tcgatctaaa aaggttagct gggtgtgtta gaagccctgc aagacgacct
     2881 gggcattcac cgcgtgcctt actacgcgta ttcttaagca ataagcgaat ataacacggt
     2941 cgatctaaaa aggttagctg ggtgtgttag aagccctgca agacgacctg ggtaccgcat
     3001 tattactcac ggtacgacgc attattactc acggtacgag gcattcaccg cgtgccttac
     3061 tacgcgtatt cttaagcaat aagcgaatat aacacggtcg atctaaaaag gttagctggg
     3121 tgtgttagaa gccctgcaag acgacctggg taccaggtaa agcggccgcg actctagatc
     3181 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     3241 cccctgaacc tgaaacataa aatgaatgca attctgcctc gagcagcgct gctcgagaga
     3301 tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     3361 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     3421 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     3481 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     3541 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     3601 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     3661 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     3721 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     3781 ttctgatttt gtaggtaacc acgtggtggt gcggaccgag cggccgcagg aacccctagt
     3841 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     3901 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     3961 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     4021 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4081 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
     4141 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4201 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     4261 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4321 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
     4381 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
     4441 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     4501 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     4561 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4621 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4681 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4741 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4981 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5041 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5101 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5161 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5221 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5281 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5341 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5401 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5461 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5521 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5581 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5641 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5701 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5761 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5821 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5881 gcgtcagacc ccg
//