LOCUS       AAV-GfaABC1D-iCreV-4x6T 6855 bp ds-DNA     circular     09-DEC-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..629
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    673..802
                     /label="AAV2 ITR "
                     /ApEinfo_revcolor="#00c72e"
                     /ApEinfo_fwdcolor="#00c72e"
     misc_feature    817..1496
                     /label="GfaABC1D"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1533..3572
                     /label="iCreV"
                     /ApEinfo_revcolor="#a400c5"
                     /ApEinfo_fwdcolor="#a400c5"
     misc_feature    2136..2156
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2136..2156
                     /label="SV40 NLS all"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2136..2159
                     /label="SV40 NLS1"
                     /ApEinfo_revcolor="#d3000b"
                     /ApEinfo_fwdcolor="#d3000b"
     misc_feature    2172..2228
                     /label="P2A min"
                     /ApEinfo_revcolor="#00c700"
                     /ApEinfo_fwdcolor="#00c700"
     misc_feature    2238..2258
                     /label="SV40 NLS all"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2238..2258
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2238..2261
                     /label="SV40 NLS1"
                     /ApEinfo_revcolor="#d3000b"
                     /ApEinfo_fwdcolor="#d3000b"
     misc_feature    3582..3601
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    3582..4110
                     /label="4x6T miR cassette"
                     /ApEinfo_revcolor="#0045c6"
                     /ApEinfo_fwdcolor="#0045c6"
     misc_feature    3602..3624
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    3625..3646
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3647..3668
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3669..3690
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    3691..3712
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3713..3734
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3735..3754
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    3755..3777
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    3778..3799
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3800..3821
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3822..3843
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    3844..3863
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    3864..3886
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    3887..3908
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3909..3930
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3931..3952
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    3958..3979
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3980..4001
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    4002..4021
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    4022..4044
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    4045..4066
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4067..4088
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    4089..4110
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     polyA_signal    4267..4747
                     /label="hGH polyA signal"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4790..4931
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#00c636"
                     /ApEinfo_fwdcolor="#00c636"
     Promoter        5777..5805
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             6045..6704
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt
      721 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      781 aactccatca ctaggggttc ctgcggccgc acgcgtacat atcctggtgt ggagtagggg
      841 acgctgctct gacagaggct cgggggcctg agctggctct gtgagctggg gaggaggcag
      901 acagccaggc cttgtctgca agcagacctg gcagcattgg gctggccgcc ccccagggcc
      961 tcctcttcat gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac
     1021 agtgcctgct tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag
     1081 cagggggctt gcattgcacc ccagcctgac agcctggcat cttgggataa aagcagcaca
     1141 gccccctagg ggctgccctt gctgtgtggc gccaccggcg gtggagaaca aggctctatt
     1201 cagcctgtgc ccaggaaagg ggatcagggg atgcccaggc atggacagtg ggtggcaggg
     1261 ggggagagga gggctgtctg cttcccagaa gtccaaggac acaaatgggt gaggggagag
     1321 ctctccccat agctgggctg cggcccaacc ccaccccctc aggctatgcc agggggtgtt
     1381 gccaggggca cccgggcatc gccagtctag cccactcctt cataaagccc tcgcatccca
     1441 ggagcgagca gagccagagc aggttggaga ggagacgcat cacctccgct gctcgcttaa
     1501 ttaatacctg aattcgatct taagccgcca ccatggccac ctctgatgaa gtcaggaaga
     1561 acctgatgga catgttcagg gacaggcagg ccttctctga acacacctgg aagatgctcc
     1621 tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa cggtacccat acactgtatg
     1681 cccctggagg atatgatatt atgggatatc tgattcagat tatgaaaaga cctaatcctc
     1741 aggtggaact gggacctgtg gatacaagcg tggccctgat tctgtgtgat ctgaaacaga
     1801 aagatacacc tattgtgtat gccagcgaag cctttctgta tatgacagga tatagcaatg
     1861 ccgaagtgct gggaagaaat tgtagatttc tgcagagccc tgatggaatg gtgaaaccta
     1921 aaagcacaag aaaatatgtg gatagcaata caattaatac aatgagaaaa gccattgata
     1981 gaaatgccga agtgcaggtg gaagtggtga attttaaaaa aaatggacag agatttgtga
     2041 attttctgac aatgattcct gtgagagatg aaacaggaga atatagatat agcatgggat
     2101 ttcagtgtga aacagaaggc ggaagcggtg gcgtgcccaa gaagaagagg aaagtcggat
     2161 ccggcagcgg cgccaccaac ttcagcctgc tgaagcaggc cggcgacgtg gaggagaacc
     2221 ccggccccct cgaggtgccc aagaagaaga ggaaagtcgg cggacatact ctgtacgctc
     2281 ccggagggta tgacataatg ggttacctaa tccagattat gaagcgtcct aatccccagg
     2341 ttgagttggg accagtggac accagcgtag ccttaattct ttgcgacctt aaacagaaag
     2401 atacaccaat agtatacgcg agtgaggctt ttttgtatat gacgggttat tccaacgcgg
     2461 aagtgttagg acgaaattgt cggtttctac aatcaccgga tgggatggtc aaaccgaaaa
     2521 gtactagaaa gtatgtggat tcgaatacaa ttaacaccat gagaaaggca atcgatagga
     2581 acgcagaagt acaagtcgag gttgtcaatt tcaaaaagaa tgggcaacgc ttcgtaaact
     2641 ttctcactat gatacctgtt agggacgaaa cgggcgagta tcgatactct atgggcttcc
     2701 aatgtgaaac agaaggtacc aacaggaaat ggttccctgc tgaacctgag gatgtgaggg
     2761 actacctcct gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa cagcacctgg
     2821 gccagctcaa catgctgcac aggagatccg gcctgcctcg cccttctgac tccaatgctg
     2881 tgtccctggt gatgaggaga atcagaaagg agaatgtgga tgctggggag agagccaagc
     2941 aggccctggc ctttgaacgc actgactttg accaagtcag atccctgatg gagaactctg
     3001 acagatgcca ggacatcagg aacctggcct tcctgggcat tgcctacaac accctgctgc
     3061 gcattgccga aattgccaga atcagagtga aggacatctc ccgcaccgat ggtgggagaa
     3121 tgctgatcca cattggcagg accaagaccc tggtgtccac agctggtgtg gagaaggccc
     3181 tgtccctggg ggttaccaag ctggtggaga gatggatctc tgtgtctggt gtggctgatg
     3241 accccaacaa ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca
     3301 cctcccaact gtccacccgg gccctggaag ggatctttga ggccacccac cgcctgatct
     3361 atggtgccaa ggatgactct gggcagagat acctggcctg gtctggccac tctgccagag
     3421 tgggtgctgc cagggacatg gccagggctg gtgtgtccat ccctgaaatc atgcaggctg
     3481 gtggctggac caatgtgaac attgtgatga actacatcag aaacctggac tctgagactg
     3541 gggccatggt gaggctgctc gaagatgggg actaacatat gggcattcac cgcgtgcctt
     3601 actacgcgta ttcttaagca ataagcgaat ataacacggt cgatctaaaa aggttagctg
     3661 ggtgtgttag aagccctgca agacgacctg cgcattatta ctcacggtac gacgcattat
     3721 tactcacggt acgaggcatt caccgcgtgc cttactacgc gtattcttaa gcaataagcg
     3781 aatataacac ggtcgatcta aaaaggttag ctgggtgtgt tagaagccct gcaagacgac
     3841 ctgggcattc accgcgtgcc ttactacgcg tattcttaag caataagcga atataacacg
     3901 gtcgatctaa aaaggttagc tgggtgtgtt agaagccctg caagacgacc tgggtaccgc
     3961 attattactc acggtacgac gcattattac tcacggtacg aggcattcac cgcgtgcctt
     4021 actacgcgta ttcttaagca ataagcgaat ataacacggt cgatctaaaa aggttagctg
     4081 ggtgtgttag aagccctgca agacgacctg ggtaccaggt aaagcggccg cgactctaga
     4141 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     4201 tccccctgaa cctgaaacat aaaatgaatg caattctgcc tcgagcagcg ctgctcgaga
     4261 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     4321 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     4381 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     4441 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     4501 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     4561 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     4621 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     4681 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     4741 ccttctgatt ttgtaggtaa ccacgtggtg gtgcggaccg agcggccgca ggaaccccta
     4801 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     4861 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4921 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4981 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5041 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
     5101 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5161 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5221 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5281 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
     5341 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
     5401 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     5461 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5521 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5581 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5641 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5701 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5761 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5821 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5881 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5941 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6001 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6061 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6121 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6181 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6241 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6301 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6361 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6421 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6481 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6541 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6601 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6661 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6781 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6841 gagcgtcaga ccccg
//