LOCUS       AAV-GfaABC1D-lck-smMyc- 6710 bp ds-DNA     circular     09-DEC-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..629
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    673..802
                     /label="AAV2 ITR "
                     /ApEinfo_revcolor="#00c72e"
                     /ApEinfo_fwdcolor="#00c72e"
     misc_feature    817..1496
                     /label="GfaABC1D"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1528..1605
                     /label="Lck"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1672..1701
                     /label="c-Myc tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     misc_feature    1672..2751
                     /label="smMyc"
                     /ApEinfo_revcolor="#4200c6"
                     /ApEinfo_fwdcolor="#4200c6"
     CDS             1705..1734
                     /label="c-Myc tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             1738..1767
                     /label="c-Myc tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2299..2328
                     /label="c-Myc tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2335..2364
                     /label="c-Myc tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2377..2406
                     /label="c-Myc tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2413..2442
                     /label="c-Myc tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2656..2685
                     /label="c-Myc tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2689..2718
                     /label="c-Myc tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2722..2751
                     /label="c-Myc tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     misc_feature    2789..2808
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    2789..3317
                     /label="4x6T miR cassette"
                     /ApEinfo_revcolor="#00c800"
                     /ApEinfo_fwdcolor="#00c800"
     misc_feature    2809..2831
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    2832..2853
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2854..2875
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    2876..2897
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    2898..2919
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    2920..2941
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    2942..2961
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    2962..2984
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    2985..3006
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3007..3028
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3029..3050
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    3051..3070
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    3071..3093
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    3094..3115
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3116..3137
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3138..3159
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    3165..3186
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3187..3208
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3209..3228
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    3229..3251
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    3252..3273
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3274..3295
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3296..3317
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    3469..4065
                     /label="WPRE"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(3945..3956)
                     /label="FactorXa site"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     polyA_signal    4119..4599
                     /label="hGH polyA signal"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4645..4786
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#00c636"
                     /ApEinfo_fwdcolor="#00c636"
     Promoter        5632..5660
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             5900..6559
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt
      721 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      781 aactccatca ctaggggttc ctgcggccgc acgcgtacat atcctggtgt ggagtagggg
      841 acgctgctct gacagaggct cgggggcctg agctggctct gtgagctggg gaggaggcag
      901 acagccaggc cttgtctgca agcagacctg gcagcattgg gctggccgcc ccccagggcc
      961 tcctcttcat gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac
     1021 agtgcctgct tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag
     1081 cagggggctt gcattgcacc ccagcctgac agcctggcat cttgggataa aagcagcaca
     1141 gccccctagg ggctgccctt gctgtgtggc gccaccggcg gtggagaaca aggctctatt
     1201 cagcctgtgc ccaggaaagg ggatcagggg atgcccaggc atggacagtg ggtggcaggg
     1261 ggggagagga gggctgtctg cttcccagaa gtccaaggac acaaatgggt gaggggagag
     1321 ctctccccat agctgggctg cggcccaacc ccaccccctc aggctatgcc agggggtgtt
     1381 gccaggggca cccgggcatc gccagtctag cccactcctt cataaagccc tcgcatccca
     1441 ggagcgagca gagccagagc aggttggaga ggagacgcat cacctccgct gctcgcttaa
     1501 ttaactaccg gactcagatc tgccaccatg ggctgtggct gcagctcaaa ccctgaagat
     1561 gactggatgg agaacattga cgtgtgtgag aactgccatt atcccccgcc gaagcttcga
     1621 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat gGAGCAAAAA
     1681 CTGATATCGG AGGAAGACTT GGCAGAGCAG AAACTGATTT CGGAAGAGGA TCTTGCCGAA
     1741 CAAAAACTGA TCAGTGAGGA AGACCTTGCC GGAGTGTCGA AGGGTGAAGA GCTGTTCACC
     1801 GGCGTGGTGC CCATCCTCGT CGAGCTGGAC GGAGATGTCA ATGGACATAA ATTCTCCGTA
     1861 CGGGGAGAGG GCGAGGGCGA CGCAACCAAC GGTAAACTGA CCCTGAAGTT CATTTGCACC
     1921 ACGGGTAAAC TGCCCGTACC CTGGCCCACC CTGGTCACAA CCTTGGGTGG CGGAGTTCAG
     1981 TGTTTTTCGC GGTATCCAGA TCACATGAAG CAGCATGACT TTTTCAAGAG CGCTATGCCC
     2041 GAGGGTTACG TTCAAGAGAG GACCATCAGT TTCAAGGACG ACGGCACCTA CAAGACACGG
     2101 GCTGAGGTGA AATTCGAGGG TGATACACTC GTGAATCGTA TCGAGCTGAA GGGCATAGAT
     2161 TTTAAGGAGG ATGGCAATAT CCTGGGCCAC AAGTTGGAGT ATAACTTCAA CAGTCACAAT
     2221 GTCTATATAA CTGCCGATAA GCAGAAGAAT GGCATTAAAG CCAATTTCAA AATTCGTCAC
     2281 AATGTCGAAG GCGGCGCAGA ACAGAAGCTC ATCAGTGAAG AGGATCTGGC CGCAGAGCAG
     2341 AAACTCATTA GCGAGGAGGA CTTGGGCGGA GGCGGCGAGC AGAAGCTGAT ATCGGAGGAA
     2401 GACCTGGCGG CCGAGCAAAA GCTCATCTCG GAGGAGGATC TCGCGGGAGG AGATGGAAGT
     2461 GTCCAGTTGG CGGATCACTA CCAGCAGAAT ACGCCAATTG GTGACGGCCC CGTCCTTCTG
     2521 CCGGATAACC ACTACCTCTC CACCCAGTCG GTGCTGAGCA AGGACCCGAA TGAAAAACGT
     2581 GACCACATGG TTCTTCTTGA GTTCGTCACG GCTGCGGGCA TCACGTTGGG CATGGACGAA
     2641 CTGTACAAGG GCGCTGAACA GAAACTTATC TCCGAGGAGG ATCTGGCGGA ACAGAAGCTG
     2701 ATCAGCGAAG AGGACCTGGC GGAGCAGAAA CTTATCAGCG AAGAGGATCT Gtaaaatagg
     2761 taaagcggcc gcgactctag atcatatggg cattcaccgc gtgccttact acgcgtattc
     2821 ttaagcaata agcgaatata acacggtcga tctaaaaagg ttagctgggt gtgttagaag
     2881 ccctgcaaga cgacctgcgc attattactc acggtacgac gcattattac tcacggtacg
     2941 aggcattcac cgcgtgcctt actacgcgta ttcttaagca ataagcgaat ataacacggt
     3001 cgatctaaaa aggttagctg ggtgtgttag aagccctgca agacgacctg ggcattcacc
     3061 gcgtgcctta ctacgcgtat tcttaagcaa taagcgaata taacacggtc gatctaaaaa
     3121 ggttagctgg gtgtgttaga agccctgcaa gacgacctgg gtaccgcatt attactcacg
     3181 gtacgacgca ttattactca cggtacgagg cattcaccgc gtgccttact acgcgtattc
     3241 ttaagcaata agcgaatata acacggtcga tctaaaaagg ttagctgggt gtgttagaag
     3301 ccctgcaaga cgacctgggt accaggtaaa gcggccgcga ctctagatca taatcagcca
     3361 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     3421 gaaacataaa atgaatgcaa ttctgcctcg agcagcgata tcataccgtc gacaatcaac
     3481 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3541 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3601 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3661 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3721 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3781 cggtggaact catcgccgcc tgtcttgccc gctgctggac aggggctcgg ctgttgggca
     3841 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     3901 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3961 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4021 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtctag agtcgacctg
     4081 cagaagcttg cctcgagcag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc
     4141 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     4201 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     4261 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     4321 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     4381 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     4441 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     4501 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     4561 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgg
     4621 tggtggtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     4681 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4741 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     4801 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     4861 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4921 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4981 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5041 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     5101 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5161 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     5221 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5281 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     5341 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     5401 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5461 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     5521 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5581 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5641 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5701 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5761 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5821 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5881 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5941 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6001 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6061 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6121 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6181 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6241 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6301 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6361 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6421 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6481 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6541 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6601 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6661 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
//