LOCUS       AAV-GfaABC1D-lck-smV5-m 6866 bp ds-DNA     circular     09-DEC-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..629
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    673..802
                     /label="AAV2 ITR "
                     /ApEinfo_revcolor="#00c72e"
                     /ApEinfo_fwdcolor="#00c72e"
     misc_feature    817..1496
                     /label="GfaABC1D"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1528..1605
                     /label="Lck"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1672..1713
                     /label="V5 tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     misc_feature    1672..2907
                     /label="smV5"
                     /ApEinfo_revcolor="#ce20bd"
                     /ApEinfo_fwdcolor="#ce20bd"
     CDS             1723..1764
                     /label="V5 tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             1774..1815
                     /label="V5 tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2347..2388
                     /label="V5 tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2398..2439
                     /label="V5 tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2458..2499
                     /label="V5 tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2509..2550
                     /label="V5 tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2764..2805
                     /label="V5 tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2815..2856
                     /label="V5 tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     CDS             2866..2907
                     /label="V5 tag"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     misc_feature    2945..2964
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    2945..3473
                     /label="4x6T miR cassette"
                     /ApEinfo_revcolor="#00c800"
                     /ApEinfo_fwdcolor="#00c800"
     misc_feature    2965..2987
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    2988..3009
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3010..3031
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3032..3053
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    3054..3075
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3076..3097
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3098..3117
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    3118..3140
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    3141..3162
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3163..3184
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3185..3206
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    3207..3226
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    3227..3249
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    3250..3271
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3272..3293
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3294..3315
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    3321..3342
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3343..3364
                     /label="miR126-3p T"
                     /ApEinfo_revcolor="#d00000"
                     /ApEinfo_fwdcolor="#d00000"
     misc_feature    3365..3384
                     /label="miR124 T"
                     /ApEinfo_revcolor="#1c3cfc"
                     /ApEinfo_fwdcolor="#1c3cfc"
     misc_feature    3385..3407
                     /label="miR137-3p T"
                     /ApEinfo_revcolor="#c13ac3"
                     /ApEinfo_fwdcolor="#c13ac3"
     misc_feature    3408..3429
                     /label="miR369-5p T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3430..3451
                     /label="miR329-3p"
                     /ApEinfo_revcolor="#ff1e09"
                     /ApEinfo_fwdcolor="#ff1e09"
     misc_feature    3452..3473
                     /label="miR431-3p T"
                     /ApEinfo_revcolor="#1bc554"
                     /ApEinfo_fwdcolor="#1bc554"
     misc_feature    3625..4221
                     /label="WPRE"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(4101..4112)
                     /label="FactorXa site"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     polyA_signal    4275..4755
                     /label="hGH polyA signal"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4801..4942
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#00c636"
                     /ApEinfo_fwdcolor="#00c636"
     Promoter        5788..5816
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             6056..6715
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt
      721 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      781 aactccatca ctaggggttc ctgcggccgc acgcgtacat atcctggtgt ggagtagggg
      841 acgctgctct gacagaggct cgggggcctg agctggctct gtgagctggg gaggaggcag
      901 acagccaggc cttgtctgca agcagacctg gcagcattgg gctggccgcc ccccagggcc
      961 tcctcttcat gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac
     1021 agtgcctgct tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag
     1081 cagggggctt gcattgcacc ccagcctgac agcctggcat cttgggataa aagcagcaca
     1141 gccccctagg ggctgccctt gctgtgtggc gccaccggcg gtggagaaca aggctctatt
     1201 cagcctgtgc ccaggaaagg ggatcagggg atgcccaggc atggacagtg ggtggcaggg
     1261 ggggagagga gggctgtctg cttcccagaa gtccaaggac acaaatgggt gaggggagag
     1321 ctctccccat agctgggctg cggcccaacc ccaccccctc aggctatgcc agggggtgtt
     1381 gccaggggca cccgggcatc gccagtctag cccactcctt cataaagccc tcgcatccca
     1441 ggagcgagca gagccagagc aggttggaga ggagacgcat cacctccgct gctcgcttaa
     1501 ttaactaccg gactcagatc tgccaccatg ggctgtggct gcagctcaaa ccctgaagat
     1561 gactggatgg agaacattga cgtgtgtgag aactgccatt atcccccgcc gaagcttcga
     1621 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat gggaaaacct
     1681 ataccgaacc ccctccttgg acttgatagt actcagcagc agggtaagcc cattccaaac
     1741 ccacttctcg gtctggatag cacacagcaa caaggtaagc ctatccctaa ccctctgttg
     1801 ggcctggact ccacgggcca aggtgtgagt aagggcgaag aactgtttac cggcgtggtc
     1861 ccaattctgg ttgagctcga cggtgacgtt aacggacaca agtttagtgt gcgcggagaa
     1921 ggagagggag atgccaccaa cggaaaattg acgctgaagt ttatatgcac cacgggaaag
     1981 ctgccggtgc cgtggccgac cttggttaca accctgggtg gcggagtcca gtgtttcagc
     2041 cgttacccag accacatgaa gcagcatgac ttctttaaga gtgctatgcc ggagggatac
     2101 gtgcaggaac ggacgatcag tttcaaagat gacggtactt acaagacgcg agccgaggtg
     2161 aagttcgagg gagacacctt ggtgaacagg atagaactta agggaattga tttcaaggaa
     2221 gacggtaata ttctgggtca caagctggaa tacaacttta acagccataa cgtctacatt
     2281 actgccgaca agcagaagaa cggcataaag gcgaatttca aaattcggca caacgtggaa
     2341 ggtggtggca agccgatccc gaatcccctg ctgggcttgg atagcacgca gcaacaggga
     2401 aagcccatcc ctaacccctt gctgggactc gacagcaccg gaggccagca aggcggtggc
     2461 aaaccgatac ccaatcccct gctcggcctg gatagcaccc agcagcaagg caagccaatc
     2521 cccaaccctc tgctgggcct cgactcgaca ggtggcgatg gaagtgttca actggcggac
     2581 cactatcagc agaatacgcc gatcggagat ggccccgtgc tgttgccgga taatcactat
     2641 ctgtcgactc agagcgttct gagtaaggac ccaaacgaaa aacgagatca catggtgttg
     2701 ttggagttcg tgaccgccgc cggcattacg ttgggaatgg acgagctgta caagggccaa
     2761 ggaggcaagc ccataccaaa tcccttgctg ggcctcgaca gcactcagca acaaggaaag
     2821 ccaataccaa atccactgtt gggcctcgac agtacgcaac agcagggcaa gcccatcccg
     2881 aacccgttgc tgggactgga tagtacctaa aataggtaaa gcggccgcga ctctagatca
     2941 tatgggcatt caccgcgtgc cttactacgc gtattcttaa gcaataagcg aatataacac
     3001 ggtcgatcta aaaaggttag ctgggtgtgt tagaagccct gcaagacgac ctgcgcatta
     3061 ttactcacgg tacgacgcat tattactcac ggtacgaggc attcaccgcg tgccttacta
     3121 cgcgtattct taagcaataa gcgaatataa cacggtcgat ctaaaaaggt tagctgggtg
     3181 tgttagaagc cctgcaagac gacctgggca ttcaccgcgt gccttactac gcgtattctt
     3241 aagcaataag cgaatataac acggtcgatc taaaaaggtt agctgggtgt gttagaagcc
     3301 ctgcaagacg acctgggtac cgcattatta ctcacggtac gacgcattat tactcacggt
     3361 acgaggcatt caccgcgtgc cttactacgc gtattcttaa gcaataagcg aatataacac
     3421 ggtcgatcta aaaaggttag ctgggtgtgt tagaagccct gcaagacgac ctgggtacca
     3481 ggtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     3541 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattct
     3601 gcctcgagca gcgatatcat accgtcgaca atcaacctct ggattacaaa atttgtgaaa
     3661 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3721 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3781 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3841 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3901 tttccgggac tttcgctttc cccctcccta ttgccacggt ggaactcatc gccgcctgtc
     3961 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4021 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4081 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4141 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4201 tttgggccgc ctccccgcct gtctagagtc gacctgcaga agcttgcctc gagcagcgct
     4261 gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     4321 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     4381 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     4441 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     4501 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     4561 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     4621 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     4681 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     4741 ttccctgtcc ttctgatttt gtaggtaacc acgtggtggt ggtgcggacc gagcggccgc
     4801 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     4861 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     4921 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     4981 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     5041 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
     5101 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5161 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5221 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5281 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5341 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
     5401 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5461 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5641 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5701 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5761 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5821 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5881 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5941 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6001 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6061 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6121 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6181 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6241 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6301 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6361 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6421 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6481 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6541 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6601 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6661 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6721 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6781 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6841 ttcgttccac tgagcgtcag accccg
//